scholarly journals THE CLINICAL DISPLAYS AND THE FUNCTIONAL ACTIVITYOF RIBOSOMAL GENES IN ASTHMATIC CHILDREN

2010 ◽  
Vol 7 (1) ◽  
pp. 53-56
Author(s):  
V P Ivanov ◽  
N V Stabrovskaya ◽  
A D Bogomazov ◽  
E V Trubnikova ◽  
V P Ivanov ◽  
...  
Keyword(s):  
Clinical Features ◽  
Functional Activity ◽  
Immunoglobulin E ◽  
Severity Index ◽  
Ribosomal Genes ◽  
Point System ◽  
Disease Manifestation ◽  
Asthmatic Children ◽  
Asthmatic Patients ◽  
Blood Sedimentation Rate

Background. To analyze the interrelations between the parameters of the functional activity of ribosomal genes and some clinical features in asthmatic children. Material and methods. 78 asthmatic patients were included in the study. Standard methods of production and colouring of cytogenetic preparations were used. The functional activity of ribosomal genes was defined on five-point system in arbitrary units. Statistical data processing was spent with the help of applied programs Statistica 6.0. Results. The data about the level of the functional activity of ribosomal genes of asthmatic children (18,57 arbitrary units) were obtained. In all patients the general functional activity of ribosomal genes parameters were correlated with the number of leukocytes and the functional activity of ribosomal genes localized in GHchromosomes parameter -with the level of immunoglobulin E. We constructed the discrimination model according to the disease severity index which included 4 significant parameters: bands number, disease manifestation age, blood sedimentation rate and active ribosomal genes copies number. Conclusion. Our data give evidence about the interrelations between the functional activity of ribosomal genes parameters and some clinical features in asthmatic children. The number of active ribosomal genes copies is considered to be one of the significant features of disease activity.

Role of Th2 type Cytokines and IgE in Asthmatic Children

2020 ◽  
Vol 13 (4) ◽  
pp. 1765-1772
Author(s):  
Assem Metwally Abo-Shanab ◽  
Hala Elnady ◽  
Iman Helwa ◽  
Rania Fawzy Mahmoud Abdelkawy ◽  
Hanan Atta ◽  
...  
Keyword(s):  
Family History ◽  
Bronchial Asthma ◽  
Immunoglobulin E ◽  
Statistical Significance ◽  
Allergic Sensitization ◽  
Th2 Cells ◽  
Asthmatic Children ◽  
Asthmatic Patients ◽  
History Of

Bronchial asthma (BA) is usually persistent through allergic sensitization, which is likely to result in bronchial hyper-responsiveness and acute bronchoconstriction due to reactions to specific and non-specific trigger stimuli. Many research focused on the role of T cells; particularly, T helper 2 (Th2) cells which linked to controlling immunoglobulin E (IgE) production due to their role in producing different cytokines, like Interleukin-13 (IL-13), in addition to influencing the function of eosinophils through the actions of IL-5. From this perspective, we decided to study the role of IgE, IL-13, and IL-5 in asthmatic children. IL-5, IL-13, and total IgE have been measured by ELISA technique in the serum of 57 children with bronchial asthma and compared to that of 20 healthy controls. Our results reported that 38/57 (66.67%) of the patient group had a family history for allergy, and parental consanguinity was found in 4/57 families (0.07%). IgE levels showed high statistical significance in asthmatic patients in comparison to controls (p = 0.00001), while IL-13 and IL-5 levels were not significantly different in patients versus control groups (P= 0.96, P = 0.81). Moreover, IgE was found significantly increased in both groups with/without family history for allergy (p = 0.009), whereas IL-13 was significantly elevated only in the group of patients with family history for allergy (0.01). This study demonstrates that asthma is strongly related to the family history of allergy, where IgE, as well as IL-13 levels, were found high in these asthmatic patients suggesting their association with underlying symptoms. Unexpectedly IL-5 was found insignificantly decreased.

scholarly journals Effects of Obesity on Airway and Systemic Inflammation in Asthmatic Children

2021 ◽  
pp. 1-11
Author(s):  
Emine Vezir ◽  
Ersoy Civelek ◽  
Emine Dibek Misirlioglu ◽  
Muge Toyran ◽  
Murat Capanoglu ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Systemic Inflammation ◽  
Adiponectin Level ◽  
Inflammatory Cells ◽  
Prick Test ◽  
Asthmatic Children ◽  
Asthmatic Patients ◽  
Reactive Protein ◽  
Type 2 Cytokines ◽  
Asthma In Children

Background: Obese asthma is a complex syndrome with certain phenotypes that differ in children and adults. There is no clear evidence regarding the presence of additive or synergistic pathological interaction between obesity and asthma in children. Objectives: Our aim was to demonstrate the interaction of obesity and asthma in children in terms of airway and systemic inflammation by a controlled observational study. Methods: Four groups were formed: asthma obese (AO), asthma nonobese (ANO), non-AO (NAO), nonasthma nonobese (NANO). Spirometry test, fractional exhaled nitric oxide (FeNO) test, skin prick test, serum inflammatory biomarkers (C-reactive protein, C3, C4, adiponectin, leptin, resistin, periostin, YKL-40, Type 1, and Type 2 cytokines) were conducted and evaluated in all participants. Sputum inflammatory cells (sputum eosinophils and neutrophils) were evaluated in patients who could produce induced sputum and obesity-asthma interactions were determined. Results: A total of 153 participants aged 6–18 years were included in the study, including the AO group (n = 46), the ANO group (n = 45), the NAO group (n = 30), and the NANO group (n = 32). IL-4 (p < 0.001), IL-5 (p < 0.001), IL-13 (p < 0.001), resistin (p < 0.001), and YKL-40 (p < 0.001) levels were higher in patients with asthma independent of obesity. The lowest adiponectin level was found in the AO group and obesity-asthma interaction was detected (p < 0.001). Sputum eosinophilia (p < 0.01), sputum neutrophilia (p < 0.01), and FeNO levels (p = 0.07) were higher in asthmatic patients independent of obesity. In the group with paucigranulocytic inflammation, resistin and YKL-40 levels were significantly lower than in the group without paucigranulocytic inflammation (p < 0.01). Conclusion: No interaction was found between obesity and asthma in terms of airway inflammation. Interaction between obesity and asthma was shown in terms of adiponectin level and resistin/adiponectin and leptin/adiponectin ratios. It was found that serum YKL-40 and resistin levels could be associated with airway inflammation.

scholarly journals Relationship of Oxidant and Antioxidant Markers to Asthma Severity in Egyptian Asthmatic Children

2017 ◽  
Vol 5 (5) ◽  
pp. 645-650 ◽  
Author(s):  
Inas R. EL-Alameey ◽  
Gihan A. Fathy ◽  
Mones M. Abu Shady ◽  
Alaa Ali ◽  
Hanan A. Fathy ◽  
...  
Keyword(s):  
Asymmetric Dimethylarginine ◽  
Strong Predictor ◽  
Predictive Biomarker ◽  
Asthma Severity ◽  
Healthy Children ◽  
Serum Concentrations ◽  
Asthmatic Children ◽  
Paraoxonase Activity ◽  
Asthmatic Patients ◽  
Anti Oxidant

BACKGROUND: Asthma is a chronic airway disease which is characterized by oxidant antioxidant imbalance with the generation of oxidative stress related mediators.AIM: The study aimed to evaluate the role of asymmetric dimethylarginine, and malondialdehyde as oxidant markers and serum paraoxonase activity as an antioxidant marker in asthma, and to determine their relationship to the asthma severity and lung function among asthmatic children in Egypt.PATIENTS AND METHODS: This case control study was conducted on sixty patients with asthma compared with sixty apparently healthy children of matched age and sex.RESULTS: Serum concentrations of oxidant markers as asymmetric dimethylarginine and malondialdehyde were significantly increased in asthmatic patients while anti-oxidant marker as paraoxonase activity was significantly decreased compared to healthy controls (P < 0.05). ANOVA test revealed highly significant elevation of the serum concentrations of oxidant markers while anti-oxidant marker was significantly decreased in severe asthmatic patients (P < 0.001) compared to the patients with moderate and mild asthma respectively. Serum malondialdehyde concentration was a strong predictor of asthma severity by multiple regression analysis (P < 0.05).CONCLUSION: The study revealed an imbalance between oxidative and antioxidant defence systems in asthmatic children. Serum concentration of malondialdehyde was the most predictive biomarker having a significant association with asthma severity.

scholarly journals Periodontal clinical features of bronchial asthma patient using inhalation drugs

2021 ◽  
Vol 33 (3) ◽  
pp. 217
Author(s):  
Maria Efrinta Ginting ◽  
Rosiliwati Wihardja ◽  
Dyah Nindita Carolina
Keyword(s):  
Periodontal Disease ◽  
Clinical Features ◽  
Bronchial Asthma ◽  
Periodontal Pocket ◽  
Periodontal Status ◽  
Asthmatic Patients ◽  
Gingival Bleeding ◽  
Periodontal Index ◽  
Asthma Patients ◽  
One Year

Introduction: Bronchial asthma patients use inhalation drugs as therapy to achieve a controlled asthma state. Inhaled corticosteroids and β2 agonists are the medication that is being used in this case. Prolonged use of inhalation drugs will affect the health of periodontal tissue. The effect of these drugs can reduce the periodontal defense mechanism and increase the risk of periodontal disease. This study aims to determine the periodontal clinical features of bronchial asthma patients using inhalation drugs. Method: This research was a descriptive study. The sample of the study were 22 females and 8 males bronchial asthma patients at the Community Lung Health Center of Bandung (Balai Besar Kesehatan Paru Masyarakat Bandung/BBKPM Bandung) who had been using inhalation drugs for at least one year. The periodontal clinical feature was assessed by employing a Community Periodontal Index (CPI) modified with probing the gingival sulcus to assess the presence of gingival bleeding, periodontal pocket, and loss of clinical attachment where then a periodontal status was obtained. The periodontal status data were then processed using the periodontitis case definitions from the Centers for Disease Control and Prevention/American Academy of Periodontology (CDC/AAP) to determine periodontal disease in asthmatics patients. Results: Clinical features of gingival bleeding were found in 26 asthmatic patients (86.7%), periodontal pockets in 18 asthmatic patients (60%), and loss of clinical attachment in 27 asthmatic patients (90%). Based on the CDC/AAP case definition, 23 asthmatic patients (76.7%) had periodontitis. Bronchial asthma patients who used inhalation drugs had a risk of periodontitis.Conclusion: Bronchial asthma patients who used inhalation drugs for at least one year had gingival bleeding and loss of clinical attachment and periodontal pocket.

THE RELATIONSHIP BETWEEN PARENTAL AND CHILDREN'S SERUM IMMUNOGLOBULIN E (IgE) AND ASTHMA

PEDIATRICS ◽  
1996 ◽  
Vol 98 (2) ◽  
pp. 329-329
Author(s):  
Stanley P. Galant
Keyword(s):  
Bronchial Asthma ◽  
Immunoglobulin E ◽  
Skin Tests ◽  
Z Score ◽  
Significant Determinant ◽  
Positive Skin ◽  
Asthmatic Children ◽  
Children With Asthma ◽  
Z Scores ◽  
The Relationship

Purpose of the Study. To examine parental factors that influence bronchial asthma and immunoglobulin E (IgE) levels in their children. Methods. The data in this study were derived from the Tucson Epidemiological Study of Airways Obstructive Diseases since 1972. Eleven evaluation periods have been performed since that time. Asthma histories were established by questionnaire. IgE levels were obtained in 738 children, 1043 fathers, and 1261 mothers. IgE levels were determined by paper radioimmunosorbent (PRIST) technique. IgE Z scores were established. An IgE Z score is the number of standard deviations IgE differs from matched age and sex-matched subjects and log corrected. Skin tests were performed by prick technique to house dust, mold, grass, tree and weed mix and appropriate controls. Results. The percentage of children with bronchial asthma increased with the presence of asthma in parents so that 11.5% of children with asthma occurred in families with no parental asthma compared with 48.6% when both parents had asthma. The rate of childhood bronchial asthma was significantly related to parental IgE levels only when the mother and father had bronchial asthma (43% vs 20%) and much less so (22% vs 10%), when there was no parental asthma. In addition, asthma in the child proved to be a highly significant determinant of the child's IgE Z score, even after correcting for parental IgE Z score. The presence of atopy (ie, positive skin tests) was also not a significant determinant of IgE levels in asthmatic children. Discussion. The authors conclude that the inheritance of IgE is only one factor related to the development of asthma, and is limited as a predictor.

scholarly journals Serum vitamin D status and biochemical parameters of asthmatic children in Sokoto Metropolis, North-western Nigeria

2019 ◽  
Vol 11 (2) ◽  
pp. 64-70
Author(s):  
Mohammed Haruna Yeldu ◽  
Mohammed Danjuma ◽  
Mainasara Abdullahi Sulaiman ◽  
Jiya Nma Muhammad ◽  
Garba Balkisu Illah ◽  
...  
Keyword(s):  
Vitamin D ◽  
Biochemical Parameters ◽  
Immunoglobulin E ◽  
Airflow Obstruction ◽  
Serum Vitamin ◽  
Hypovitaminosis D ◽  
Asthma Severity ◽  
Vitamin D Status ◽  
Asthmatic Children ◽  
Serum Vitamin D

Background: Asthma is a common chronic disorder of the airways that is complex and characterized by variable and recurring symptoms. It is characterized by the occurrence of bronchial hyper responsiveness, airflow obstruction and an underlying inflammation. Several studies have proposed the association of vitamin D with increased incidence of asthma symptoms. Aims and Objectives: This study aimed at determining the serum vitamin D status and biochemical parameters among asthmatic children in Sokoto-Nigeria. Materials and Methods: The study population consisted of 120 subjects including 80 asthmatic children and 40 age-and sex-matched apparently healthy controls from two tertiary Hospitals in Sokoto Metropolis. Serum vitamin D, calcium, parathyroid hormone (PTH), immunoglobulin E (IgE) and magnesium were measured using standard techniques. Results: Mean serum vitamin D and magnesium were significantly (p ˂ 0.05) lower, while serum PTH and IgE were significantly higher (p˂0.001) in asthmatic children than in controls. Vitamin D insufficiency was found to be prevalent among the asthmatic children. There was no significant relationship between the low level of vitamin D and asthma severity. Conclusion: This study observed that, hypovitaminosis D and hypomagnesaemia occurred among asthmatic children, and asthma was more prone in the younger children than in their older counterpart. It is recommended that in order to avoid or limit the rate of children having asthmatic episodes, vitamin D and magnesium supplements should be used in the management of asthmatic children.

scholarly journals Nasal Cytokine Profiles of Patients Hospitalised with Respiratory Wheeze Associated with Rhinovirus C

Viruses ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1038 ◽  
Author(s):  
Chisha T. Sikazwe ◽  
Ingrid A. Laing ◽  
Allison Imrie ◽  
David W. Smith
Keyword(s):  
Therapeutic Interventions ◽  
Upper Respiratory Tract ◽  
Th17 Response ◽  
Asthmatic Children ◽  
Asthmatic Patients ◽  
School Aged Children ◽  
Cytokine Profiles ◽  
Nasal Swabs ◽  
Inflammatory Pattern ◽  
Rhinovirus C

Background: Rhinovirus C is an important pathogen of asthmatic and non-asthmatic children hospitalised with episodic wheeze. Previous studies on other respiratory viruses have shown that several host cytokines correlate with duration of hospitalisation, but this has yet to be investigated in children with RV-C infection. We determined the nasal cytokine profiles of these children and investigated their relationship with RV-C load and clinical outcome. Flocked nasal swabs were collected from children aged 24–72 months presenting to the Emergency Department at Princess Margaret Hospital with a clinical diagnosis of acute wheeze and an acute upper respiratory tract viral infection. RV-C load was determined by quantitative RT-PCR and cytokine profiles were characterised by a commercial human cytokine 34-plex panel. RV-C was the most commonly detected virus in pre-school-aged children hospitalised with an episodic wheeze. RV-C load did not significantly differ between asthmatic and non-asthmatic patients. Both groups showed a Th2-based cytokine profile. However, Th17 response cytokines IL-17 and IL-1β were only elevated in RV-C-infected children with pre-existing asthma. Neither RV-C load nor any specific cytokines were associated illness severity in this study. Medically attended RV-C-induced wheeze is characterised by a Th2 inflammatory pattern, independent of viral load. Any therapeutic interventions should be aimed at modulating the host response following infection.

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