scholarly journals Polymorphism of the cytokine genes (H4, ИМ) and peculiarities of immune response depending on the control over atopic bronchial asthma in children

2013 ◽  
Vol 10 (2) ◽  
pp. 23-28
Author(s):  
M V Smolnikova ◽  
S V Smirnova ◽  
O S Tyutina ◽  
S V Bychkovskaya
Keyword(s):  
Bronchial Asthma ◽  
Cellular Immunity ◽  
Control Group ◽  
Healthy Children ◽  
Nucleotide Polymorphisms ◽  
Promoter Regions ◽  
Cytokine Genes ◽  
Humoral And Cellular Immunity ◽  
Atopic Bronchial Asthma ◽  
Cell Counts

Background. The aim of this study was to investigate the main indices of humoral and cellular immunity, the levels of cytokines (IL-2, IL-4, IL-10, TNFa) and polymorphism of promoter regions of cytokine genes (IL4 and IL10) in children with atopic bronchial asthma (ABA) with different levels of disease control. Methods. We have analyzed 110 children with ABA - controlled (CABA, n=59) and uncontrolled (UABA, n=51) and healthy children (control group, n=52). Parameters of cellular immunity were determined by fluorescence microscopy using monoclonal antibodies to surface receptors. Parameters of humoral immunity and cytokine levels in the samples of serum were measured by ELISA. Genotyping of single-nucleotide polymorphisms in the IL4 (С-590Т) and IL10 (С597А) genes was performed by PCR. Results. The level of TNFa and the relative amount of CD8+ cells was increased, while the counts of CD3+ cells and the relative amount of CD4+ cells was decreased in UABA as compared to CABA. In CABA, lower concentration of the IL-10 in serum associated with the IL10 A-597 allele was observed. The IL4 T-590 allele tends to be associated with non-controlled ABA. Conclusion. The level of TNFa, and the CD3+, CD4+ and CD8+ cell counts were identified as markers of uncontrolled course of ABA in children. Polymorphic variants of IL4 and IL10 genes can be considered as candidate markers of uncontrolled ABA.

scholarly journals PHENOTYPES OF EOSINOPHILS IN THE DIAGNOSIS OF ATOPIC BRONCHIAL ASTHMA IN CHILDREN

2019 ◽  
Vol 29 (3) ◽  
pp. 79-83
Author(s):  
Elena Asiryn ◽  
Pavel Novikov ◽  
Volha Matsiushchanka ◽  
Laimutė Vaidelienė ◽  
Goda Misevičiūtė ◽  
...  
Keyword(s):  
Bronchial Asthma ◽  
Allergic Diseases ◽  
Control Group ◽  
Healthy Children ◽  
Absolute Level ◽  
Relative Level ◽  
Relative Index ◽  
Asthmatic Children ◽  
Atopic Bronchial Asthma ◽  
The Absolute

The aim of the research was to study the phenotype of eosinophils in children with atopic bronchial asthma and their role as diagnostic criteria of the disease. Materials and methods. We have examined 130 children aged from 6 to 18 years old with atopic bronchial asthma. The control group consisted of 40 healthy children from 6 to 18 years old, who didn’t have allergic diseases. During the research the relative and absolute levels of eosinophils, the level of eosinophils carrying FcεRI and FcεRII (CD23) – receptors were established. Results. The relative level of eosinophils in asthmatic children was 6.00% [3.00; 8.00], the absolute level – 417.00 cells/μL [232.00; 636,00], which is significantly higher compared to control group, where the relative level was 2.50% [1.00; 3.00%] and the absolute level was 166.50 cells/μl [86.00; 213.00] (p<0.001). The level of eosinophils carrying the FcεRI receptor was 420,00 cells/μl [250,00; 660,00], which is significantly higher than in the control group – 200,00 cells/μl [140,00; 240.00] (p<0.001). The relative level of eosinophils carrying CD23 receptor on their surface was 62.20% [35.40; 76.60] and the absolute level –223.37 cells/μl [105.30; 375.24]. The results are significantly higher than those of the control group: 25.45% [14.30; 30,60] (p<0,001), and 30,88 cells/μl [25,63; 42.84] (p <0.0001) respectively. It was found in the ROC-analysis that there is high evidence of presence of atopic bronchial asthma if absolute levels of eosinophils carrying CD23 receptor was 73.008 cells/μl or more or the relative index – 35.10% and more. Conclusions. The relative and absolute levels of eosinophils, as well as eosinophils carrying FcεRI and CD23 receptors are statistically significantly higher in atopic bronchial asthma than in control group patients. Increased number or relative index (73.008 cells/μl or ≥ 35.10% respectively) of eosinophils carrying CD 23 receptors can be considered as a diagnostic criterion of atopic bronchial asthma.

scholarly journals Initial vegetative tone in mild atopic asthma in children

2021 ◽  
Vol 12 (2) ◽  
pp. 13-18
Author(s):  
Vladimir N. Buryak ◽  
Tatyana I. Antonova ◽  
Maria V. Dudko ◽  
Inna V. Malysheva ◽  
Kirill K. Shepelenko
Keyword(s):  
Nervous System ◽  
Autonomic Nervous System ◽  
Bronchial Asthma ◽  
The Body ◽  
Atopic Asthma ◽  
World Health ◽  
Control Group ◽  
Healthy Children ◽  
Autonomic Nervous ◽  
Atopic Bronchial Asthma

Background. In most industrialized countries, allergic diseases affect up to 20% of the population. This pathology belongs to the most common in children: according to the World Health Organization, more than 15% of the world's child population suffers from it. In recent years, there has been a significant increase in the frequency and more severe course of these diseases, in connection with which they are considered in modern society as a major medical and social problem. Thus, the prevalence of bronchial asthma, according to domestic and foreign authors, ranges from 0.2 to 8.1%. Purpose. In order to clarify the role of the autonomic nervous system in the genesis of the mild course of atopic bronchial asthma in childhood, the features of the interaction of the sympathetic and parasympathetic divisions of the autonomic nervous system in the examined children were clarified. Materials and methods. 126 children aged 10 to 14 years were examined. Atopic bronchial asthma was diagnosed in 91 children. At the same time, 61 of them were diagnosed with an intermittent course, 30 a mild persistent course of the disease. The control group consisted of 35 healthy children also aged 10 to 14 years. Complaints, anamnesis data were studied in all children, an objective and generally accepted laboratory and instrumental examination was carried out. All examined children underwent daily monitoring of the electrocardiogram, according to the results of which, based on the analysis of time and frequency indicators of heart rate variability, a variant of the initial autonomic tone was established. Results. In children with atopic bronchial asthma, both with intermittent and mild persistent course, an absolute or relative dominance of sympathetic influences was revealed against the background of varying degrees of decrease in parasympathetic activity, which was interpreted as a compensatory reaction of the body in response to chronic allergic inflammation.

ОЦЕНКА ЦИТОКИНОВОГО ПРОФИЛЯ СЫВОРОТКИ КРОВИ И СУБПОПУЛЯЦИЙ Т-ЛИМФОЦИТОВ У ДЕТЕЙ С АЛЛЕРГИЧЕСКИМИ ЗАБОЛЕВАНИЯМИ ОРГАНОВ ДЫХАНИЯ

2019 ◽  
pp. 52-60
Author(s):  
E.V. Prosekova ◽  
A.I. Turyanskaya ◽  
N.G. Plekhova ◽  
M.S. Dolgopolov ◽  
V.A. Sabynych
Keyword(s):  
Allergic Rhinitis ◽  
Bronchial Asthma ◽  
Allergic Inflammation ◽  
Allergic Diseases ◽  
Control Group ◽  
Healthy Children ◽  
Serum Cytokine ◽  
Immunological Parameters ◽  
Low Level ◽  
Level Of Significance

Расширение спектра изучаемых клонов Тхелперов определило более сложные иммунные механизмы реализации аллергического воспаления. Цель. Характеристика показателей и взаимосвязей цитокинового профиля сыворотки и субпопуляционного состава Тлимфоцитов периферической крови у детей с бронхиальной астмой и аллергическим ринитом. Материалы и методы. Проведено комплексное обследование 150 детей в возрасте 311 лет с верифицированным диагнозом бронхиальной астмы, аллергического ринита и 30 здоровых сверстников. Иммунологические параметры крови оценивали методом проточной цитометрии, концентрации интерлейкинов и IgE в сыворотке крови определяли методом твердофазного иммуноферментного анализа. При статистической обработке использовали программы Statistica 10 с критическим уровнем значимости р0,05. Результаты. У детей с аллергическими заболеваниями в сыворотке крови определены высокие уровни содержания интерлейкинов4, 8, 13, 17А, сопоставимый с показателями группы контроля уровень IL17F и низкое содержание IFNy. При бронхиальной астме и аллергическом рините у детей выявлено увеличение количества CD3CD8CD45RO, CD3CD8CD45RACD45RO Тлимфоцитов и CD3CD4 Тхелперов и повышение количество Th17 при снижении CD3CD4CD45RO клеток памяти. В группе здоровых детей популяция Th17 составляла 9,491,6, у детей с аллергическими заболеваниями количество данных клеток было значимо выше 14,50,77 (р0,001). Анализ сывороточного содержания цитокинов у детей с изолированным течением БА и в сочетании с аллергическим ринитом выявил разнонаправленные корреляции, отличающиеся по силе и направленности от таковых в группе здоровых детей. Заключение. У детей при изолированном течении бронхиальной астмы и в сочетании с аллергическим ринитом выявлены: сопоставимое с показателями здоровых детей количество CD3CD4 Тклеток, дисбаланс в субпопуляционном составе Тхелперов за счет преобладания Th2 и Th17, активация синтеза IL17A, IL4, IL8, IL13, низкий уровень сывороточного IFNy, изменения силы и направленности взаимосвязей цитокинового профиля и спектра субпопуляций Тлимфоцитов.Expansion of the range of examined Thelper clones has determined more complex immune mechanisms for the implementation of allergic inflammation. Objective. To characterize the parameters and relationships between the serum cytokine profile and Tlymphocyte subpopulation in peripheral blood of children with bronchial asthma and allergic rhinitis. Materials and methods. 150 children aged between 311 years old with bronchial asthma, and allergic rhinitis and 30 healthy volunteers were examined. Immunological parameters were assessed by flow cytometry, the concentration of serum interleukins and IgE were determined by means of enzymelinked immunosorbent assay. Statistical analysis was performed with Statistica 10 program with a critical level of significance p0.05. Results. High levels of interleukins 4, 8, 13, 17A were determined, IL7F level was not significantly different from that in control group and low level of IFNy was found in the serum of children with allergic diseases. The number of CD3CD8CD45RO, CD3CD8CD45RACD45RO Tlymphocytes, CD3CD4 Thelper cells and Th17 were increased and at the same time CD3CD4CD45RO memory cells were decreased In bronchial asthma and allergic rhinitis children. Number of Th17 cells in healthy children was 9.491.6, in allergic children it was significantly higher 14.50.77 (p0.001). Analyses of serum cytokine count in children with isolated BA and in association with allergic rhinitis revealed multidirectional correlations differing in strength and direction from those in the group of healthy children. Conclusion. In children with isolated bronchial asthma and associated with allergic rhinitis the following parameters were found: CD3CD4 Tcells count was comparable to that in healthy children, the imbalance of Thelper subpopulation: prevalence of Th2 and Th17, activation of IL17A, IL4, IL8, IL13 synthesis and low level of serum IFNy.

scholarly journals Characteristics of the eosinophilic fenotype of bronchial asthma in children

2017 ◽  
pp. 52-57
Author(s):  
E Asiryan ◽  
N Titova
Keyword(s):  
Bronchial Asthma ◽  
Skin Tests ◽  
Control Group ◽  
Ige Receptor ◽  
Positive Skin ◽  
Ige Antibodies ◽  
Total Ige ◽  
Atopic Bronchial Asthma ◽  
Ige Receptors ◽  
High Level

The purpose of the work is clinical and laboratory examination of children with an eosinophilic phenotype of bronchial asthma. Materials and methods. 160 children aged 5 to 18 years were examined: 130 patients with atopic bronchial asthma, 30 children of the control group. During the work, the level of eosinophils, the level of eosinophils carrying FcεRI+ and CD23+IgE+ receptor, the level of total IgE, IgE antibodies to domestic and epidermal allergens in the blood serum were determined, clinical and anamnestic data were studied. Results. In children with atopic bronchial asthma, the relative and absolute content of eosinophils, as well as eosinophils carrying FcεRI+ and CD23+IgE+ receptors, is statistically significantly higher than in the control group (p<0.001). In 71 (54.62%) children with bronchial asthma, eosinophilia was detected, with 58 (81.69%) patients having a high level of eosinophils bearing the FcεRI + receptor and 56 (78.87%) children with a high level of eosinophils carrying CD23+IgE+ receptor. In the group of children with bronchial asthma, a correlation was established between the level of total IgE and the level of eosinophils carrying FcεRI+ (r=+0.389; p<0.01) and CD23+IgE+ receptors (r=+ 0.677; p<0.0001). In children with eosinophilia, in most cases, the hereditary nature of the disease is observed, the first manifestations of allergy are already present in the first year of life, concomitant allergopathology has been established. The optimal effect in the treatment of patients with eosinophilia was obtained with the appointment, as a basic therapy, of inhaled glucocorticosteroids, as well as short-acting β2-agonists for arresting episodes of bronchial obstruction. In most patients with asthma who have eosinophilia, serum levels of IgE antibodies to Dermatophagoides pteronyssinus, to the coat of the cat and the dog's coat are revealed, and positive skin scarification tests with these allergens are detected. Conclusion. In children with atopic bronchial asthma, there are signs of an eosinophilic phenotype: eosinophilia, high expression of FcεRI+ and CD23+IgE+ receptors, increased levels of total IgE and IgE antibodies, positive skin tests with domestic and epidermal allergens, hereditary predisposition, effectiveness of inhaled glucocorticosteroids.

scholarly journals Humoral and Cellular Immunity to Candida albicans in Patients with Bronchial Asthma.

1992 ◽  
Vol 31 (6) ◽  
pp. 766-769 ◽  
Author(s):  
Yoshiro TANIZAKI ◽  
Hikaru KITANI ◽  
Morihiro OKAZAKI ◽  
Takashi MIFUNE ◽  
Fumihiro MITSUNOBU
Keyword(s):  
Candida Albicans ◽  
Bronchial Asthma ◽  
Cellular Immunity ◽  
Humoral And Cellular Immunity

scholarly journals Neuropsychiatric features of children with systemic somatovegetative disorders (by the example of atopic dermatitis, bronchial asthma and gastroesophageal reflux disease)

2019 ◽  
Vol 10 (3) ◽  
pp. 40-48
Author(s):  
T. S. Petrenko ◽  
K. Yu. Retyunskiy ◽  
M. D. Borovskikh ◽  
D. R. Devyatkina ◽  
A. V. Pereshitova ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Bronchial Asthma ◽  
Reflux Disease ◽  
Energy Functional ◽  
Control Group ◽  
Healthy Children ◽  
Central Nervous System Damage ◽  
Neuropsychological Disorders

Objective: to study the clinical neuropsychiatric and neuropsychological features of children suffering from systemic somatovegetative disorders.Materials and methods: the study involved children from 5 to 12 years old with an established diagnosis of bronchial asthma — 108 children; atopic dermatitis — 105 children; gastroesophageal reflux disease — 112 children; the control group consisted of 60 same age healthy children. All children underwent clinical-anamnestic, neurological, psychopathological and neuropsychological research.Results: the children with systemic somatovegetative disorders have a significantly high incidence of pathogenic factors of central nervous system damage in early stages of ontogenesis (pathology of pregnancy and childbirth). Early sensory and motor deprivation due to somatic suffering aggravates neuropsychiatric deficiency. Neuropsychological disorders were predominantly represented by a violation of the perception of their body, lack of kinesthetic and motor functions, spatial and quasi-spatial representations.Conclusion: the clinical dynamics of neuropsychiatric disorders in children with systemic somatovegetative disorders corresponds to the dynamics of residual cerebral organic impairment with a stage-age changes of syndromes. The revealed neuropsychological disorders correspond to preferential damage to the first (energy) functional block of the brain.

scholarly journals Analysis of associations of polymorphisms in the genes coding for L4, IL10, IL13 with the development of atopic bronchial asthma and its remission

2019 ◽  
pp. 87-91
Author(s):  
Yu.V. Zhorina ◽  
S.O. Abramovskikh ◽  
G.L. Ignatova ◽  
O.G. Ploshchanskay
Keyword(s):  
Bronchial Asthma ◽  
Molecular Mechanisms ◽  
Control Group ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Atopic Bronchial Asthma ◽  
Genotype Frequencies ◽  
Urban Sample ◽  
Allele Specific ◽  
Polymerase Chain

Bronchial asthma is a multifactorial disease underpinned by chronic inflammation. The atopic phenotype of BA implies the presence of similar molecular mechanisms of pathogenesis between the patients. The aim of this study was to analyze the associations between the development of atopic BA/its remission and the following polymorphisms of interleukin genes: IL4 (rs2243250; C-589T), IL10 (rs1800896; G-1082A; rs1800872; C-592A), and IL13 (rs20541; Arg130Gln). Using allele-specific polymerase chain reaction (PCR), we studied the listed SNPs in the mixed urban sample of patients with BA (n = 53) and the controls (n = 30) residing in South Ural. The analysis revealed that genotype АА of IL10 (rs1800872) occurred more frequently in the control group (23.3%) than in the patients with atopic BA (5.7%) (OR = 0.197; 95% CI [0.047–0.832]; р = 0.031). No differences in genotype frequencies were observed between the patients with atopic BA and the controls for other studied polymorphisms. Our study failed to demonstrate the association of the listed polymorphisms and BA remission.

scholarly journals Serum Lp-PLA2 Level Predicting Coronary Artery Lesions in Children with Kawasaki Disease

2021 ◽  
Vol 24 (4) ◽  
pp. E611-E618
Author(s):  
Xiong Zhang ◽  
Ya-Wang Shao ◽  
Ya-Lan Zhang ◽  
Yi Liu
Keyword(s):  
Coronary Artery ◽  
Kawasaki Disease ◽  
Color Doppler ◽  
Intima Media Thickness ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Children ◽  
Coronary Artery Lesions ◽  
Cell Counts ◽  
Ifn Γ

Background: Kawasaki disease (KD) is an inflammatory disease associated with coronary vasculitis in children. In this study, we explored the correlation between Lipoprotein associated phospholipase A2 (Lp-PLA2) and coronary artery lesions (CAL) in children with KD. Methods: Ninety-three children with KD were divided into a normal coronary artery (NCA, 54 cases) group and coronary artery lesions (CAL, 39 cases) group, according to the results of echocardiography. Another 42 healthy children were selected as the control group. The serumal levels of Lp-PLA2, Interferon-γ(IFN-γ) and Interleukin-6 (IL-6) were determined by using an enzyme-linked immunosorbent assay. In addition, erythrocyte sedimentation rate (ESR) and serum C-reactive protein (CRP) level were analyzed. The left main coronary artery (LMCA), diameters of left anterior descending coronary artery (LADC), right proximal coronary artery (PRCA), and carotid intima-media thickness (IMT) were obtained by color Doppler ultrasound. The correlation between the above indexes and KD was analyzed. Results: The levels of white blood cell counts (WBC), ESR, CRP, IFN-γ, IL-6 and Lp-PLA2 as well as IMT were significantly increased in KD children (P < 0.05), and the levels of CRP, IFN-γ, IL-6 and Lp-PLA2 as well as IMT in the CAL group increased more significantly (P < 0.05). An increasing trend also has been described in the diameters of LMCA, LADC and PRCA for KD children with CAL compared with with NCA. The results of logistic regression analysis showed that the elevated levels of CRP, IFN-γ, IL-6 and Lp-PLA2 were independent risk factors for KD with CAL. Correlation analysis showed that Lp-PLA2 level was positively correlated with the levels of IFN-γ, IL-6 and CRP in CAL group and NCA group (respectively, all P < 0.01). In addition, a similar correlation was also described between Lp-PLA2 level and the diameters of LMCA, LADC and PRCA in CAL group (respectively, all P < 0.01). Conclusion: Lp-PLA2 may participate in the pathological mechanism of KD. Detection of the serum Lp-PLA2 level can be used in the diagnosis of KD disease and the assessment of coronary artery lesions in KD children.

scholarly journals Clinical features of bronchial asthma course in children of Vinnytska oblast, depending on age and level of the disease control

2019 ◽  
Vol 23 (2) ◽  
pp. 300-303
Author(s):  
V. M. Zhmurchuk
Keyword(s):  
Disease Control ◽  
Clinical Features ◽  
Bronchial Asthma ◽  
Statistical Processing ◽  
Epidemiological Studies ◽  
Atopic Asthma ◽  
Control Group ◽  
Healthy Children ◽  
Nasal Breathing ◽  
Children With Asthma

According to various epidemiological studies, the prevalence of bronchial asthma (BA) in children in Ukraine ranges from 5 to 12%. Despite numerous studies of etiological factors, mechanisms of pathogenesis, the creation of international and national programs, until now it is not possible to take control of the morbidity and course of asthma. Therefore, the purpose of our study is to determine the clinical features of the course of bronchial asthma in children, depending on the age and level of disease control. We examined 227 children with asthma aged 6 to 17 years. In the objective examination, respiratory failure, shortness of breath, dry cough, nasal breathing difficulties, wheezy breathing, emphysema, bloating of the chest, were taken into account. The statistical processing of the results was carried out using the IBM SPSS Statistics, version 20 (2013), using parametric and non-parametric methods for evaluating the results. Under supervision were 181 boys (79.73±0.5%), from the total number of examined patients with asthma and 46 girls (20.27±1.98%). The control group included 40 practically healthy children aged 6 to 17 years. It was established that the persistent course of light and medium severity was noted 2.5 times more often than the severe course of asthma, and also 2.4 and 2 times more often (p≤0.01) in the age group of 6–11 years, unlike children 12–17 years old. Severe exacerbations were twice as likely to occur in patients with asthma-mediated etiology, and lungs — in patients with atopic asthma. Uncontrolled levels of the disease were noted 1.6–2 times more often in non-atopic form of asthma, than in its other variants. Thus, among children with asthma, mixed asthma and non-atopic asthma with an uncontrolled course, there is a clear need to find factors that are trigger in the development of exacerbations of the disease, determine their duration and severity, which helps to prevent the progression of asthma and avoid complications.

scholarly journals The efficiency of non-specific inhaled immunotherapy with ruzam in patients with atopic bronchialasthma

2011 ◽  
Vol 8 (4) ◽  
pp. 49-54
Author(s):  
D V Terekhov ◽  
Nataliya Mikhaylovna Nenasheva ◽  
D V Terekhov ◽  
N M Nenasheva
Keyword(s):  
Blood Serum ◽  
Bronchial Asthma ◽  
Bronchial Hyperresponsiveness ◽  
Allergic Inflammation ◽  
Induced Sputum ◽  
Control Group ◽  
Asthma Control Test ◽  
Open Label ◽  
Atopic Bronchial Asthma ◽  
Markers Of Inflammation

Background. To study the efficacy and safety of inhaled form of ruzam in adult patients with persistent mild and moderate atopic bronchial asthma (ba). Materials and methods. a placebo-controlled prospective, randomized, open label study of an efficacy of ruzam a solution for inhalations (in bottles on 2,5 or 5 ml) on 2,5 ml onсe a day through nebulizer during 2 weeks was conducted in adult asthmatic patients. The efficacy of therapy was evaluated by dynamics of symptoms and requirement in ƒ2-agonists, besides PEfr, spirometry, bronchial hyperresponsiveness (bhr), asthma control test (aCT ), level of the markers of the allergic inflammation were performed. Results. reduction of bа symptoms, increase morning PEfr, decrease of bhr and NОex level in patients who received ruzam compared with control group was observed. achievement of clinical control of bа in patients treated by ruzam was accompanied by statistically significant positive dynamics of markers of inflammation (ifNg, ECP) in blood serum and in supernatant of the induced sputum. it testifies about anti-inflammatory influence of ruzam. Conclusion. inhaled therapy with ruzam in a complex with iNCs basic therapy is an effective and safe method of treatment of persistent atopic bronchial asthma. This combination allows to reach clinical and functional control of ba faster and to reduce inflammation markers in blood serum and induced sputum.

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