scholarly journals Formation and survival of in vitro protocormos of Cerrado Orchid of Goiás

2020 ◽  
Vol 13 (6) ◽  
pp. 1
Author(s):  
D. C. S. Lemos ◽  
G. R. Sousa ◽  
A. D. V. Souza ◽  
J. B. A. França ◽  
M. R. Rosa ◽  
...  
Keyword(s):  
Bacterial Contamination ◽  
Sodium Hypochlorite Solution ◽  
Ms Medium ◽  
In Vitro Development ◽  
Federal Institute ◽  
In The Wild ◽  
Vitro Development ◽  
Mature Seeds ◽  
Pot Plants

The Cerrado has wide plant diversity, including orchids. These plants need symbiosis with fungi for their germination to occur in the wild. In vitro or asbiotic cultivation is an alternative for the production of seedlings of this species. This diversity of orchids makes it much appreciated as pot plants, landscaping, with high commercial value. The objective of this work was to carry out the study for the development of a protocol for the germination and in vitro development of orchids. The experiment was carried out at the LABIOTEC Biotechnology Laboratory of the Federal Institute Goiano Campus Urutaí-GO. Catasetum sp native capsules from Goiás State, containing mature seeds were collected and disinfected in 50% sodium hypochlorite solution for 30 minutes and in laminar flow were washed four times in distilled and autoclaved water. They were then seeded in vitro in 258 mL glass vials containing 30 mL of MS medium enriched with vitamins and 0.0 Tryptone; 1.0; 2.0 and 3.0 g L-1. The pH of the medium was adjusted to 5.2 ± 0.1. The average time for germination and development of the protocorms varied from 3 to 7 days after sowing for the species under study. The dosage of 2.0 g L-1 is efficient for the development and maintenance of in vitro Catasetum protocorms up to 333 days. There was no development of shoot of Catasetum seedlings in any of the evaluated treatments. It was observed that the 3.0 g L-1 Tryptone dosage presented higher rates of fungal and bacterial contamination and oxidation

scholarly journals Potássio, cálcio e magnésio na micropropagação de plantas tropicais ornamentais

2016 ◽  
Vol 22 (1) ◽  
pp. 74
Author(s):  
Viviane Amaral Toledo Coelho ◽  
Gabrielen De Maria Gomes Dias ◽  
Cleber Lázaro Rodas ◽  
Moacir Pasqual ◽  
Mozart Martins Ferreira ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
In Vitro Cultivation ◽  
Ms Medium ◽  
Original Formulation ◽  
In Vitro Development ◽  
Number Of Leaves ◽  
Alpinia Purpurata ◽  
Vitro Development ◽  
Etlingera Elatior

Considering the increase in economical importance of species belong to Zingiberaceae family for floriculture and the advantages of techniques applied to micropropagation of these plants, it is necessary to carry out studies about growth of species in these conditions. The aim of this study was to evaluate the in vitro development of Etlingera elatior, Zingiber spectabile and Alpinia purpurata under different ratios of K, Ca and Mg. The treatments were based on MS medium with K: Mg ratios (mmol L-1) of: 22K:1Mg, 20K:3Mg, 16K:7Mg, 12K:11Mg, 8K:15Mg 4K:19Mg; K:Ca ratios (mmol L-1) of: 22K:4Ca, 20K:6Ca, 16K:10Ca, 12K:14Ca, 8K:16Ca and 4K:22Ca; with five replications, and two seedlings per pot. Explants used were obtained from in vitro rhizomes, kept in a room at a temperature of 25 ± 2°C under irradiance 52W m-2 s-1 and 16 hours photoperiod. After 80 days, seedlings were harvest and have the about number of leaves, height and length of root measured. After, seedlings were separated in shoots and roots, dried and carried out chemical analysis of plant tissue. It is possible to improve the Zingiberaceae plants growth during the micropropagation, changing the concentrations of original formulation MS medium. For K are recommended concentrations of 22 mmol L-1 to Etlingera elatior and Zingiber spectabile cultivation and 16 mmol L-1 for Alpinia purpurata. For Ca, we recommend the concentrations of 4 mmol L-1 for Etlingera elatior and Zingiber spectabile and from 8 to 10 mmol L-1 for Alpinia purpurata. A higher concentration of Mg for in vitro cultivation Etlingera elatior is required. For Zingiber spectabile and Alpinia purpurata it was recommended the proposed merger among MS original concentration of 3 mmol L-1 of this macronutrient.

In vitro development of human triploid zygotes reconstructed by pronuclear transfer

2002 ◽  
Vol 78 ◽  
pp. S180-S181
Author(s):  
John Zhang ◽  
Yi Ming Shu ◽  
Lewis C Krey ◽  
Hui Liu ◽  
Guang Lun Zhuang ◽  
...  
Keyword(s):  
In Vitro Development ◽  
Pronuclear Transfer ◽  
Vitro Development

Gallic acid promotes the in vitro development of sheep secondary isolated follicles involving the phosphatidylinositol 3-kinase pathway

2021 ◽  
pp. 106767
Author(s):  
Gizele A.L. Silva ◽  
Luana B. Araújo ◽  
Larissa C.R. Silva ◽  
Bruna B. Gouveia ◽  
Ricássio S. Barberino ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Phosphatidylinositol 3 Kinase ◽  
In Vitro Development ◽  
Vitro Development ◽  
Kinase Pathway ◽  
Phosphatidylinositol 3

scholarly journals Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets

2021 ◽  
Vol 22 (16) ◽  
pp. 8367
Author(s):  
Hien Lau ◽  
Shiri Li ◽  
Nicole Corrales ◽  
Samuel Rodriguez ◽  
Mohammadreza Mohammadi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oxygen Consumption ◽  
Insulin Secretion ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
In Vitro Development ◽  
Porcine Islets ◽  
Vitro Development

Pre-weaned porcine islets (PPIs) represent an unlimited source for islet transplantation but are functionally immature. We previously showed that necrostatin-1 (Nec-1) immediately after islet isolation enhanced the in vitro development of PPIs. Here, we examined the impact of Nec-1 on the in vivo function of PPIs after transplantation in diabetic mice. PPIs were isolated from pancreata of 8–15-day-old, pre-weaned pigs and cultured in media alone, or supplemented with Nec-1 (100 µM) on day 0 or on day 3 of culture (n = 5 for each group). On day 7, islet recovery, viability, oxygen consumption rate, insulin content, cellular composition, insulin secretion capacity, and transplant outcomes were evaluated. While islet viability and oxygen consumption rate remained high throughout 7-day tissue culture, Nec-1 supplementation on day 3 significantly improved islet recovery, insulin content, endocrine composition, GLUT2 expression, differentiation potential, proliferation capacity of endocrine cells, and insulin secretion. Adding Nec-1 on day 3 of tissue culture enhanced the islet recovery, proportion of delta cells, beta-cell differentiation and proliferation, and stimulation index. In vivo, this leads to shorter times to normoglycemia, better glycemic control, and higher circulating insulin. Our findings identify the novel time-dependent effects of Nec-1 supplementation on porcine islet quantity and quality prior to transplantation.

In vitro development of plants from microspores of rice

In Vitro ◽  
1977 ◽  
Vol 13 (8) ◽  
pp. 484-489 ◽  
Author(s):  
Chi-Chang Chen
Keyword(s):  
In Vitro Development ◽  
Vitro Development
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