scholarly journals Analysis of antioxidant enzymes and total antioxidant capacity in the midgut of Africanized Apis mellifera selected for tolerance to the neonicotinoid thiamethoxam

2020 ◽  
Vol 13 (10) ◽  
pp. 50
Author(s):  
W. C. S. Pizzaia ◽  
T. O. Diniz ◽  
N. C. Pereira ◽  
B. G. Silva ◽  
A. S. Khatlab ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Apis Mellifera ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Total Antioxidant

scholarly journals Effect of the French Oak Wood Extract Robuvit on Markers of Oxidative Stress and Activity of Antioxidant Enzymes in Healthy Volunteers: A Pilot Study

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Martina Horvathova ◽  
Zuzana Orszaghova ◽  
Lucia Laubertova ◽  
Magdalena Vavakova ◽  
Peter Sabaka ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Antioxidant Capacity ◽  
Healthy Volunteers ◽  
Total Antioxidant Capacity ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Markers Of Oxidative Stress

We examinedin vitroantioxidant capacity of polyphenolic extract obtained from the wood of oakQuercus robur(QR), Robuvit, using TEAC (Trolox equivalent antioxidant capacity) method and the effect of its intake on markers of oxidative stress, activity of antioxidant enzymes, and total antioxidant capacity in plasma of 20 healthy volunteers. Markers of oxidative damage to proteins, DNA, and lipids and activities of Cu/Zn-superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined in the erythrocytes. We have found anin vitroantioxidant capacity of Robuvit of 6.37 micromole Trolox equivalent/mg of Robuvit. One month intake of Robuvit in daily dose of 300 mg has significantly decreased the serum level of advanced oxidation protein products (AOPP) and lipid peroxides (LP). Significantly increased activities of SOD and CAT as well as total antioxidant capacity of plasma after one month intake of Robuvit have been shown. In conclusion, we have demonstrated for the first time that the intake of Robuvit is associated with decrease of markers of oxidative stress and increase of activity of antioxidant enzymes and total antioxidant capacity of plasmain vivo.

scholarly journals White Tea Intake Abrogates Markers of Streptozotocin-Induced Prediabetes Oxidative Stress in Rat Lungs’

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3894
Author(s):  
Ana C. Silveira ◽  
Luís Rato ◽  
Pedro Fontes Oliveira ◽  
Marco G. Alves ◽  
Branca M. Silva
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Chemical Properties ◽  
Histone H2a ◽  
Protein Nitration ◽  
White Tea ◽  
Lung Dysfunction ◽  
Total Antioxidant

Prediabetes (PrDM) is a prodromal stage of diabetes mellitus (DM) with an increasing prevalence worldwide. During DM progression, individuals gradually develop complications in various organs. However, lungs are suggested to be affected later than other organs, such as the eyes, heart or brain. In this work, we studied the effects of PrDM on male Wistar rats’ lungs and whether the regular consumption of white tea (WTEA) for 2 months contributes to the improvement of the antioxidant profile of this tissue, namely through improved activity of the first line defense antioxidant enzymes, the total antioxidant capacity and the damages caused in proteins, lipids and histone H2A. Our data shows that PrDM induced a decrease in lung superoxide dismutase and glutathione peroxidase activities and histone H2A levels and an increase in protein nitration and lipid peroxidation. Remarkably, the regular WTEA intake improved lung antioxidant enzymes activity and total antioxidant capacity and re-established the values of protein nitration, lipid peroxidation and histone H2A. Overall, this is the first time that lung is reported as a major target for PrDM. Moreover, it is also the first report showing that WTEA possesses relevant chemical properties against PrDM-induced lung dysfunction.

scholarly journals Comparison of the effects of three different Baccaurea angulata whole fruit juice doses on plasma, aorta and liver MDA levels, antioxidant enzymes and total antioxidant capacity

2017 ◽  
Vol 57 (5) ◽  
pp. 1817-1828 ◽  
Author(s):  
Muhammad Ibrahim ◽  
Maryam Abimbola Mikail ◽  
Idris Adewale Ahmed ◽  
Norazlanshah Hazali ◽  
Mohammad Syaiful Bahari Abdul Rasad ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Fruit Juice ◽  
Total Antioxidant

scholarly journals P378 Oxidative status and antioxidant capacity in children and adults with IBD: preliminary results of the OxIBDiet trial

2021 ◽  
Vol 15 (Supplement_1) ◽  
pp. S392-S393
Author(s):  
S Gatti ◽  
L Cianfruglia ◽  
T Galeazzi ◽  
A Palpacelli ◽  
S Quattrini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Antioxidant Capacity ◽  
Protein Oxidation ◽  
Total Antioxidant Capacity ◽  
Oxidative Status ◽  
Healthy Controls ◽  
Preliminary Results ◽  
Total Antioxidant

Abstract Background Redox imbalance is involved in several aspects of inflammatory bowel disease (IBD). The OxIBDiet (NCT04513015) is a multicentre, 2-phases project involving IBD children and adults with the following aims: 1. To evaluate oxidative status of IBD subjects. 2. To estimate the effects of an antioxidant diet in IBD patients. Preliminary results are shown in this abstract. Methods The total antioxidant capacity, lipid peroxidation and the degree of protein oxidation were measured respectively through the ferric reducing ability of plasma (FRAP, µmol/equivalent FeSO4), serum levels of the thiobarbituric acid reactive substances (TBARs, µmol MDA) and advanced Oxidation Protein Products (AOPP, µmol/g protein). Reactive oxygen species (ROS, Arbitrary Units) and activities (nmol/min/mg of protein) of the main antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione S-Transferase (GST) and catalases (CAT) were evaluated in PMNs cells. Oxidative stress markers were correlated with demographic variables and clinical parameters. Results Fifteen adults (mean age: 36.2 ±11.4 years, 47% female, 67% in remission) and thirty five children (mean age: 14.13 ±2.2 years, 60% female, 65% in remission) with IBD have been enrolled so far. FRAP was significantly reduced in IBD children compared to healthy controls (median values: 212.1 vs. 248.3, p=0.0001) and to IBD adults (median value: 281, p<0.001), while no difference was observed between adults with IBD and the control group (p= 0.1). ROS levels did not differ in IBD children compared to adults and controls. The activity of GPX and CAT enzymes was increased in children with IBD in comparison to controls (p:0.02 and 0.001, respectively) while the activity of the other enzymes (GST, GR and SOD) and levels of lipid peroxidation and protein oxidation was not different between the 2 groups. Overall in the IBD group (children plus adults) FRAP was positively correlated with age (r=0.40, p=0.006), male gender (r=0.33, p=0.03) and use of biologics (r=0.47, p=0.001) and inversely correlated to disease activity based on clinical scores (r= -0.38, p=0.009). No correlation was found between FRAP and serum C-reactive protein or calprotectin levels. Conclusion The total antioxidant capacity (FRAP) is significantly impaired in IBD children respect to IBD adults and to healthy controls, thus suggesting an early involvement of oxidative stress in IBD pathogenesis. Moreover, the activity of the main antioxidant enzymes (GPx and CAT) in IBD children is increased, as a possible compensation for redox imbalance. Final results will clarify the involvement of antioxidant cascade in IBD pathogenesis and in therapeutic approach.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

Summer Day/Night Gender Differences in Serum Total Antioxidant Capacity as a Methodological Pitfall in Human Antioxidant Research

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Armando L. Morera-Fumero ◽  
Pedro Abreu-Gonzalez ◽  
Lourdes Fernandez- Lopez ◽  
Estefanía Díaz-Mesa ◽  
Maria del Rosario Cejas-Mendez
Keyword(s):  
Gender Differences ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Total Antioxidant
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