scholarly journals Box-Behnken Design Assisted Protein Precipitation Optimization for Simultaneous Determination of Metformin Hydrochloride and Alogliptin Benzoate in Plasma along with Pharmacokinetic Application

2021 ◽  
Vol 83 (4) ◽  
Author(s):  
H. P. Patel ◽  
D. P. Patel ◽  
J. R. Christian ◽  
P. A. Shah ◽  
K. G. Patel ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Protein Precipitation ◽  
Metformin Hydrochloride ◽  
Box Behnken Design

Simultaneous Determination of Ritonavir and Lopinavir in Human Plasma after Protein Precipitation and LC-MS-MS

2010 ◽  
Vol 71 (9-10) ◽  
pp. 815-824 ◽  
Author(s):  
Rajasekhar Damaramadugu ◽  
Jaswanthkumar Inamadugu ◽  
Ravi Kanneti ◽  
Srinivasulu Polagani ◽  
Venkateswarlu Ponneri
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Protein Precipitation

scholarly journals Column High-Performance Liquid Chromatographic Method for the Simultaneous Determination of Rosiglitazone and Metformin in a Pharmaceutical Preparation

2009 ◽  
Vol 92 (1) ◽  
pp. 119-124 ◽  
Author(s):  
Azzam R Ali ◽  
Ibrahim I Duraidi ◽  
Munib M Saket ◽  
Eyad S M Abu-Nameh
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Pharmaceutical Preparation ◽  
High Performance Liquid Chromatographic ◽  
Metformin Hydrochloride ◽  
Dihydrogen Phosphate ◽  
Simple Method ◽  
Combination Tablet ◽  
Tablet Dosage

Abstract An efficient, sensitive, and simple method was developed for the simultaneous determination of rosiglitazone and metformin hydrochloride in a combination tablet dosage form by column high-performance liquid chromatography. The mobile phase used was ammonium dihydrogen phosphate adjusted to pH 5.25 with sodium hydroxide. The limits of detection and quantitation were in the range of 0.51.6 g/mL, respectively, for metformin hydrochloride, and 0.002010.0067 g/mL, respectively, for rosiglitazone. The linearity was studied in the concentration range of 0.120.31 g/mL for rosiglitazone and 30.676.7 g/mL for metformin hydrochloride. The recovered amounts of metformin hydrochloride and rosiglitazone were 100103.8 and 101103.7, respectively.

scholarly journals A VALIDATED STABILITY INDICATING ULTRA-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS DETERMINATION OF METFORMIN HYDROCHLORIDE AND EMPAGLIFLOZIN IN BULK DRUG AND TABLET DOSAGE FORM

2017 ◽  
Vol 9 (3) ◽  
pp. 45 ◽  
Author(s):  
N. Madana Gopal ◽  
C. Sridhar
Keyword(s):  
Simultaneous Determination ◽  
Dosage Form ◽  
Accurate Method ◽  
Array Detector ◽  
Metformin Hydrochloride ◽  
Control Analysis ◽  
International Conference ◽  
Stability Indicating ◽  
Tablet Dosage

Objective: To develop a simple, precise, accurate, method was developed and validated for analysis of metformin hydrochloride (MET) and empagliflozin in (EMPA) in bulk and tablet dosage form.Methods: The method used a reverse phase column, dikma C18 (50×2.1 mm, 1.8 μ), a mobile phase comprising of phosphate buffer (pH-3): methanol (30:70 v/v) flow rate of 1.0 ml/min and a detection wavelength of 240 nm using a photodiode array detector. The proposed method was validated for various parameters like linearity, precision, accuracy, robustness, ruggedness, detection, quantification limits, stability studies, formulation analysis as per International Conference on Harmonization (ICH) guidelines.Results: The retention time was found to be 1.189 min and 1.712 min for MET and EMPA respectively. The proposed method was found to be having linearity in the concentration range of 500-2500 μg/ml for MET (r2=0.989) and 5-25 μg/ml for EMPA (r2=0.994), respectively. The mean % recoveries obtained were found to be 100.35-100.48% for MET and 99.80-101.30% for EMPA respectively. Stress testing which covered acid, base, peroxide, photolytic and thermal degradation was performed on under test to prove the specificity of the method and the degradation was achieved. The developed method has been statistically validated according to International Conference on Harmonization (ICH) guidelines.Conclusion: Thus, the proposed method can be successfully applied for the stability indicating the simultaneous determination of MET and EMPA in bulk and combined tablet dosage form and in the routine quality control analysis.

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