Optimization and validation of a MEKC method assisted by Box–Behnken Design for fast and simultaneous determination of nitrendipine and atenolol in new antihypertensive combination tablets

2013 ◽  
Vol 5 (13) ◽  
pp. 3353 ◽  
Author(s):  
You Li ◽  
Ji Li ◽  
Shanqiao Chen ◽  
Dan Yang ◽  
Chengchu Liu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Box Behnken Design

scholarly journals Eco-friendly extraction and simultaneous determination of two coumarins in Justicia pectoralis (Acanthaceae)

Rodriguésia ◽  
2020 ◽  
Vol 71 ◽  
Author(s):  
Alessandra Ribeiro Sartor Lima ◽  
Luan Augusto Cândido Lôbo ◽  
Thaísa Lorrana da Cruz Costa ◽  
Josana de Castro Peixoto ◽  
Leonardo Luiz Borges ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Ethanol Concentration ◽  
Folk Medicine ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Herbal Products ◽  
Box Behnken Design ◽  
Sedative Effects ◽  
Predicted Values

Abstract Justicia pectoralis (Acanthaceae) is employed in folk medicine for its analgesic, anti-inflammatory and sedative effects and to treat respiratory diseases. It is known for properties of its coumarins, 1,2-benzopyrone and umbelliferone. A green, simple, fast, and inexpensive ultrasound-assisted extractive (UAE) method for extracting umbelliferone and 1,2-benzopyrone from Justicia pectoralis was optimized. Additionally, a HPLC analytical method was developed and validated for the simultaneous determination of both coumarins. The Box-Behnken design and response surface methodology were used to evaluate the UAE process. Ethanol concentration, extraction time, plant-to-solvent ratio were the independent variables studied and the coumarin content was the dependent one. The HPLC-UV/VIS method was validated in terms of recovery, linearity, accuracy, precision and robustness, proving to be valuable for the quality control of Justicia pectoralis extract and in the development of its herbal products. Results show that the optimal UAE conditions were: ethanol concentration of 15% (w/w), extraction time of 34 min and plant-to-solvent ratio of 0.1 g/mL. The predicted values of coumarin contents (22.16 µg/mL - umbelliferone and 163.86 µg/mL - benzopyrone) were determined under the optimal UAE conditions and proved that UAE is an efficient and eco-friendly extractive process for the production of aerial part extracts from Justicia pectoralis.

A general computer program for the extended plate overlap algorithm

1978 ◽  
Vol 48 ◽  
pp. 287-293 ◽  
Author(s):  
Chr. de Vegt ◽  
E. Ebner ◽  
K. von der Heide
Keyword(s):  
Computer Program ◽  
Simultaneous Determination ◽  
General Computer Program ◽  
General Computer

In contrast to the adjustment of single plates a block adjustment is a simultaneous determination of all unknowns associated with many overlapping plates (star positions and plate constants etc. ) by one large adjustment. This plate overlap technique was introduced by Eichhorn and reviewed by Googe et. al. The author now has developed a set of computer programmes which allows the adjustment of any set of contemporaneous overlapping plates. There is in principle no limit for the number of plates, the number of stars, the number of individual plate constants for each plate, and for the overlapping factor.

Sign in / Sign up

Export Citation Format

Share Document