scholarly journals Molecular detection and identification of a ‘Candidatus Phytoplasma solani’-related strain associated with pumpkin witches’ broom in Xinjiang, China

2021 ◽  
Vol 60 (1) ◽  
pp. 63-68
Author(s):  
Xu WANG ◽  
Chun-Guang WANG ◽  
Xiao-Yan LI ◽  
Zheng-Nan LI
Keyword(s):  
Molecular Detection ◽  
Fragment Length Polymorphism ◽  
First Record ◽  
Related Strain ◽  
Autonomous Region ◽  
Detection And Identification ◽  
The World ◽  
Xinjiang Uyghur Autonomous Region ◽  
Restriction Fragment Length

Pumpkin plants showing symptoms of witches’ broom (PuWB) were observed in Xinjiang Uyghur autonomous region, China, in September 2018. A phytoplasma was detected in symptomatic plants by PCR amplifying portions of the 16S ribosomal and tuf genes. In addition, the phylogeny based on these genes sequencing indicated that the PuWB strain clusters with ‘Candidatus Phytoplasma solani’ (subgroup 16SrXII-A). Furthermore, based on in silico and in vitro restriction fragment length polymorphism analyses, the PuWB phytoplasma was confirmed as a ‘Ca. P. solani’-related strain. This was the first record of the occurrence of phytoplasma presence in pumpkins in China, and the first record of 16SrXII phytoplasma infecting pumpkins in the world.

First record of the genus Microstenus Szépligeti (Hymenoptera: Ichneumonidae: Cryptinae) from China, with description of one new species and a key to world species

Zootaxa ◽  
2020 ◽  
Vol 4838 (4) ◽  
pp. 594-600
Author(s):  
TAO LI ◽  
SHU-PING SUN ◽  
MAO-LING SHENG
Keyword(s):  
New Species ◽  
First Record ◽  
World Species ◽  
Autonomous Region ◽  
Guangxi Zhuang Autonomous Region ◽  
The World ◽  
One New Species ◽  
A New Species

The genus Microstenus Szépligeti, 1916 (Hymenoptera: Ichneumonidae: Cryptinae) is newly recorded from China based on a new species, Microstenus rufithorax Sheng, Li & Sun, sp. nov., collected from Guangxi Zhuang Autonomous Region, Oriental part of China. Illustrations of the new species are provided. A key to the world species of this genus is also provided. 

Settler Colonialism and the Path toward Cultural Genocide in Xinjiang

2021 ◽  
Vol 13 (1) ◽  
pp. 9-19
Author(s):  
Michael Clarke
Keyword(s):  
Ethnic Identity ◽  
Cultural Identity ◽  
Education System ◽  
Settler Colonialism ◽  
Religious Minority ◽  
Autonomous Region ◽  
Counter Terrorism ◽  
Cultural Genocide ◽  
The World ◽  
Xinjiang Uyghur Autonomous Region

Abstract The Xinjiang Uyghur Autonomous Region (xuar) is the site of the largest mass repression of an ethnic and/or religious minority in the world today characterised by extra-judicial detention of Uyghurs in ‘re-education’ centres, pervasive surveillance, and repression of Uyghur ethnic identity. While Beijing frames such draconian measures as necessary ‘counter-terrorism’ measures, the intersection between concern for the ‘welfare’ of subject populations and the desire to eradicate ‘defective’ elements of cultural identity central to the ‘re-education’ system in Xinjiang betray the fundamentally colonial nature of the Chinese Communist Party’s (ccp) endeavours in the region.

Human in vitro cell lines verification by minisatellite DNA restriction fragment length polymorphism

1998 ◽  
Vol 128 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Maria Mańczak ◽  
Magdalena Zielińska-Dawidziak ◽  
Magdalena Prussak ◽  
Joanna Dubis ◽  
Anita Kość ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Cell Lines ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Minisatellite Dna ◽  
Dna Restriction ◽  
In Vitro Cell Lines ◽  
Restriction Fragment Length

Buffalopox: an emerging and re-emerging zoonosis

2007 ◽  
Vol 8 (1) ◽  
pp. 105-114 ◽  
Author(s):  
R. K. Singh ◽  
M. Hosamani ◽  
V. Balamurugan ◽  
V. Bhanuprakash ◽  
T. J. Rasool ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Molecular Biology ◽  
Length Polymorphism ◽  
Type Species ◽  
Fragment Length Polymorphism ◽  
Genetic Relationships ◽  
Viral Pathogenesis ◽  
Diagnostic Methods ◽  
The World ◽  
Restriction Fragment Length

AbstractOutbreaks of buffalopox or pox-like infections affecting buffaloes, cows and humans have been recorded in many parts of the world. Since the first outbreak in India, a large number of epidemics have occurred. Unlike in the previous years, generalized forms of the disease are now rare; however, there are severe local forms of the disease affecting the udder and teats, leading to mastitis thereby undermining the productivity of milk animals. The causative agent buffalopox virus (BPXV) is a member of theOrthopoxvirus, and is closely related to Vaccinia virus (VACV), the type-species of the genus. Earlier studies with restriction fragment length polymorphism and recent investigations involving sequencing of the genes that are essential in viral pathogenesis have shown that BPXV is phylogenetically very closely related to VACV and may be considered as a clade of the latter. The review discusses the epidemiology, novel diagnostic methods for the disease, and molecular biology of the virus, and infers genetic relationships of BPXV with other members of the genus.

scholarly journals Development of a 16S rRNA Gene Primer and PCR-Restriction Fragment Length Polymorphism Method for Rapid Detection of Members of the Genus Megasphaera and Species-Level Identification

2011 ◽  
Vol 77 (15) ◽  
pp. 5533-5535 ◽  
Author(s):  
Akihiro Ohnishi ◽  
Shinko Abe ◽  
Shiho Nashirozawa ◽  
Sayaka Shimada ◽  
Naoshi Fujimoto ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Direct Detection ◽  
Rrna Gene ◽  
Content Type ◽  
Detection And Identification ◽  
Restriction Fragment Length

ABSTRACTThe genusMegasphaerais relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism (RFLP) analyses to detect and identify the members ofMegasphaera. Direct detection and identification were achieved for environmental samples and isolates.

scholarly journals Identification of Polymorphisms of the CSN2 Gene Encoding β-Casein in Greek Local Breeds of Cattle

2021 ◽  
Vol 8 (11) ◽  
pp. 257
Author(s):  
Dionysios Antonopoulos ◽  
Despina Vougiouklaki ◽  
George P. Laliotis ◽  
Theofania Tsironi ◽  
Irene Valasi ◽  
...  
Keyword(s):  
Fragment Length Polymorphism ◽  
Restriction Enzymes ◽  
The Other ◽  
Holstein Cows ◽  
Gene Encoding ◽  
Blood Samples ◽  
Agarose Gels ◽  
Detection And Identification ◽  
Pcr Product ◽  
Restriction Fragment Length

This research focused on the detection and identification of genetic polymorphisms in exon 7 of the β-casein CSN2 gene in blood samples from Greek Holstein cows and from local breeds of cattle, such as Vrachykeratiki, Katerinis, and Sykias. For this purpose, DNA was isolated from 780 blood samples obtained from Greek Holstein cows, 86 from three local breeds of cattle, namely Brachyceros, Katerinis, and Sykias, and 14 from Greek buffalo. The desired region of exon 7 was amplified by PCR, resulting in 121 and 251 bp products in bovine and buffalo samples. The PCR product was digested with restriction fragment length polymorphism (RFLP) on agarose gels. The restriction enzymes DdeI and TaqI were used. All of the blood samples had the amplified size. The results showed that 74.4% of the Greek Holstein cows had the A2A2 β-casein genotype, the three native breads Vrachykeratiki had 57.7%, and the other two had 100% of the A2A2 β-casein. From the 14 Greek buffalo ,100% had the A2A2 β-casein.

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