scholarly journals Marker Assisted Gene Pyramiding for Bacterial Blight Resistance in Some Improved Rice Genotypes

2021 ◽  
Vol 3 (1) ◽  
pp. 10-21
Author(s):  
Ahmed A. ◽  
Ramalingam J. ◽  
Isong A. ◽  
Mala K. K. ◽  
Mbe J. O.
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Gene Pyramiding ◽  
F1 Hybrids ◽  
Blight Resistance ◽  
Polymorphic Markers ◽  
Parental Genome ◽  
Bacterial Blight Resistance ◽  
Foreground Selection ◽  
Rice Genotypes

Bacterial blight is one of the major diseases of rice, causing huge economic loss to the rice farmers around the world. In the present study, marker assisted selection (MAS) was used to pyramid broad spectrum resistance genes (Xa33 and Xa38) into two improved rice genotypes (ADT 47 and ASD 16) which already have some bacterial blight resistance genes (xa5, xa13 and Xa21). The rice genotypes FBRI-15 and PR114 were used as donors of Xa33 and Xa38 genes respectively. The marker RMWR7.1 linked to Xa33 and the sequenced-tagged site marker Os04g53050-1 specific to Xa38 were used for foreground selection. True F1 hybrids were selected using the polymorphic markers. Out of one hundred and thirty-seven SSR markers, forty-two were polymorphic for improved ADT 47 x FBRI-16 and forty-six were polymorphic for Improved ASD 16 x PR114. The polymorphic markers were used for background selection. Foreground selection revealed that a single F1 plant was heterozygote in Improved ADT 47 X FBRI-15 whereas two plants were heterozygotes in Improved ASD 16 x PR114. At BC1F1, one and three plants were found to be heterozygous respectively for Improved ADT 47 x FBRI-15 and Improved ASD 16 x PR114. At BC2F1, single plant each was found heterozygous from the two crosses. In the final BC3F1 population, one heterozygote each was obtained from the two crosses. In BC3F1, plant number 11 had 92.80% parental genome recovery in the Improved ADT 47 x FBRI-15, whereas plant no 16 had 91.33% parental genome recovery in the Improved ASD 16 x PR114

scholarly journals Screening of Restorable WA-CMS Rice Genotypes for Bacterial Blight (BB) Resistance

2020 ◽  
pp. 45-52
Author(s):  
Surabhi Sinha ◽  
Sweta Sinha ◽  
Mankesh Kumar ◽  
Suresh Prasad Singh ◽  
Anand Kumar ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Hybrid Breeding ◽  
Blight Resistance ◽  
Bacterial Blight Resistance ◽  
Local Varieties ◽  
Resistant Genes ◽  
Rice Yields ◽  
Parental Material ◽  
Rice Genotypes

Aim: To screen and evaluate rice genotypes which were found to be having restorability for WA-CMS cytoplasm for their further use in the hybrid breeding program. Study Design: Laboratory-experimental design was used in the study. Place and Duration of the Study: The genotypes used in the study were comprised of promising lines from various experiments, local varieties and landraces, etc. The study was conducted during June 2017 to May 2018. Methodology: In the present study, 55 rice lines which were found to have restorable capacity for WA-CMS system were screened for 4 Bacterial Blight resistance genes viz. Xa4, xa5, xa13, Xa21, against most prevalent races of the pathogen throughout the country, using PCR based molecular markers namely, MP1+MP2, xa5 multiplex, xa13 prom and pTA248, respectively. Results: Bacterial Blight (BB) is considered to be one of the most important diseases of the rice crop. As hybrids are one of the most viable options to increase rice yields, the parental material used for the development of hybrids, must be having genes which show resistance against BB. Out of 55 restorer lines, 43 genotypes amplified for Xa4, 7 genotypes namely R. Bhagwati, IRBB5-9, Narendra Usar Dhan 3, Pratikhya, IR 88964-24-2-1-4, IR 94314-20-2-1B, HHZ 5-DT8-DT1-Y1 showed positive bands for xa5, 3 genotypes namely PAU 3220, N. Usar Dhan 3 and Pratikhya showed positive bands for xa13. However, only one genotype namely Pratikhya amplified for Xa21. In combinations, Narendra Usar Dhan 3 amplified for xa5 and xa13. Pratikhya was the only genotype found to have all 4 BB (Xa4, xa5, xa13, Xa21) resistance genes under consideration.  Conclusion: Genotypes having different BB resistant genes in combinations along with good capability of restoration for prevalent WA-CMS system can further be used as male parent in the hybrid breeding programme for development of BB resistant hybrids.

scholarly journals Pyramiding Bacterial Blight Resistance Genes in Tainung82 for Broad-Spectrum Resistance Using Marker-Assisted Selection

2020 ◽  
Vol 21 (4) ◽  
pp. 1281 ◽  
Author(s):  
Yu-Chia Hsu ◽  
Chih-Hao Chiu ◽  
Ruishen Yap ◽  
Yu-Chien Tseng ◽  
Yong-Pei Wu
Keyword(s):  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Grain Quality ◽  
Gene Pyramiding ◽  
High Yield ◽  
Blight Resistance ◽  
Recurrent Parent ◽  
Bacterial Blight Resistance ◽  
Resistant Genes

Tainung82 (TNG82) is one of the most popular japonica varieties in Taiwan due to its relatively high yield and grain quality, however, TNG82 is susceptible to bacterial blight (BB) disease. The most economical and eco-friendly way to control BB disease in japonica is through the utilization of varieties that are resistant to the disease. In order to improve TNG82’s resistance to BB disease, five bacterial blight resistance genes (Xa4, xa5, Xa7, xa13 and Xa21) were derived from a donor parent, IRBB66 and transferred into TNG82 via marker-assisted backcrossing breeding. Five BB-resistant gene-linked markers were integrated into the backcross breeding program in order to identify individuals possessing the five identified BB-resistant genes (Xa4, xa5, Xa7, xa13 and Xa21). The polymorphic markers between the donor and recurrent parent were used for background selection. Plants having maximum contribution from the recurrent parent genome were selected in each generation and crossed with the recipient parent. Selected BC3F1 plants were selfed in order to generate homozygous BC3F2 plants. Nine pyramided plants, possessing all five BB-resistant genes, were obtained. These individuals displayed a high level of resistance against the BB strain, XF89-b. Different BB gene pyramiding lines were also inoculated against the BB pathogen, resulting in more than three gene pyramided lines that exhibited high levels of resistance. The five identified BB gene pyramided lines exhibited yield levels and other desirable agronomic traits, including grain quality and palatability, consistent with TNG82. Bacterial blight-resistant lines possessing the five identified BB genes exhibited not only higher levels of resistance to the disease, but also greater yield levels and grain quality. Pyramiding multiple genes with potential characteristics into a single genotype through marker-assisted selection can improve the efficiency of generating new crop varieties exhibiting disease resistance, as well as other desirable traits.

scholarly journals Epidemiological Effect of Gene Deployment Strategies on Bacterial Blight of Rice

1997 ◽  
Vol 87 (1) ◽  
pp. 66-70 ◽  
Author(s):  
Hafiz U. Ahmed ◽  
Maria R. Finckh ◽  
Rizal F. Alfonso ◽  
Christopher C. Mundt
Keyword(s):  
Resistance Genes ◽  
Partial Resistance ◽  
Bacterial Blight ◽  
Blight Resistance ◽  
Resistant Line ◽  
Bacterial Blight Resistance ◽  
Progress Curve ◽  
Two Component ◽  
The Mean ◽  
Bacterial Blight Of Rice

Experiments were conducted in farmers' fields at two locations of the irrigated lowlands of Laguna province in southern Luzon island, Philippines, during the wet seasons of 1993 and 1994. Nine rice populations were studied including pure stands, two-component mixtures, two-gene combinations of backcrossed lines containing varying combinations of the bacterial blight resistance genes Xa-4, xa-5, and Xa-10, and a non-isogenic cultivar containing Xa-4 and partial resistance to bacterial blight. The area under the disease progress curve (AUDPC) of both gene combinations studied was significantly less than the single most effective gene of each combination deployed singly. A mixture of a susceptible and a resistant line expressed an AUDPC significantly less than the mean of its component pure stands, but two other mixtures did not. The cultivar IR20, which contains both Xa-4 and partial resistance, reduced the AUDPC by about two-thirds as compared with IR-BB4, which contains Xa-4 and little or no partial resistance.

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