Multiplex PCR genotyping for five bacterial blight resistance genes applied to marker-assisted selection in rice (Oryza sativa)

2016 ◽  
Vol 135 (3) ◽  
pp. 309-317 ◽  
Author(s):  
Ruishen Yap ◽  
Yu-Chia Hsu ◽  
Yong-Pei Wu ◽  
Yann-Rong Lin ◽  
Chei-Wei Kuo
Keyword(s):  
Oryza Sativa ◽  
Multiplex Pcr ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Blight Resistance ◽  
Bacterial Blight Resistance ◽  
Pcr Genotyping

Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR

1997 ◽  
Vol 95 (3) ◽  
pp. 313-320 ◽  
Author(s):  
N. Huang ◽  
E. R. Angeles ◽  
J. Domingo ◽  
G. Magpantay ◽  
S. Singh ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Blight Resistance ◽  
Bacterial Blight Resistance

scholarly journals Pyramiding Bacterial Blight Resistance Genes in Tainung82 for Broad-Spectrum Resistance Using Marker-Assisted Selection

2020 ◽  
Vol 21 (4) ◽  
pp. 1281 ◽  
Author(s):  
Yu-Chia Hsu ◽  
Chih-Hao Chiu ◽  
Ruishen Yap ◽  
Yu-Chien Tseng ◽  
Yong-Pei Wu
Keyword(s):  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Bacterial Blight ◽  
Grain Quality ◽  
Gene Pyramiding ◽  
High Yield ◽  
Blight Resistance ◽  
Recurrent Parent ◽  
Bacterial Blight Resistance ◽  
Resistant Genes

Tainung82 (TNG82) is one of the most popular japonica varieties in Taiwan due to its relatively high yield and grain quality, however, TNG82 is susceptible to bacterial blight (BB) disease. The most economical and eco-friendly way to control BB disease in japonica is through the utilization of varieties that are resistant to the disease. In order to improve TNG82’s resistance to BB disease, five bacterial blight resistance genes (Xa4, xa5, Xa7, xa13 and Xa21) were derived from a donor parent, IRBB66 and transferred into TNG82 via marker-assisted backcrossing breeding. Five BB-resistant gene-linked markers were integrated into the backcross breeding program in order to identify individuals possessing the five identified BB-resistant genes (Xa4, xa5, Xa7, xa13 and Xa21). The polymorphic markers between the donor and recurrent parent were used for background selection. Plants having maximum contribution from the recurrent parent genome were selected in each generation and crossed with the recipient parent. Selected BC3F1 plants were selfed in order to generate homozygous BC3F2 plants. Nine pyramided plants, possessing all five BB-resistant genes, were obtained. These individuals displayed a high level of resistance against the BB strain, XF89-b. Different BB gene pyramiding lines were also inoculated against the BB pathogen, resulting in more than three gene pyramided lines that exhibited high levels of resistance. The five identified BB gene pyramided lines exhibited yield levels and other desirable agronomic traits, including grain quality and palatability, consistent with TNG82. Bacterial blight-resistant lines possessing the five identified BB genes exhibited not only higher levels of resistance to the disease, but also greater yield levels and grain quality. Pyramiding multiple genes with potential characteristics into a single genotype through marker-assisted selection can improve the efficiency of generating new crop varieties exhibiting disease resistance, as well as other desirable traits.

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