scholarly journals UJI DAYA HAMBAT EKSTRAK ETANOL DAUN PANGI (Pangium edule Reinw. ex Blume) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Pseudomonas aeruginosa

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 275
Author(s):  
Glorya Sakul ◽  
Herny E. I. Simbala ◽  
Gerald Rundengan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
North Sulawesi ◽  
Staphyloccocus Aureus ◽  
Inhibitory Strength

ABSTRACTOne of the natural ingredients that are often used as a medicinal plants is Pangi (Pangium edule Reinw. Ex Blume) plant. The part of pangi that are often used are leaves, which is known have antibacterial activity. North Sulawesi people empirically use this plant as food and to cure various diseases such as treating itching on the skin caused by bacteria found on the skin. The aim of this research was to determine and study the strength of antibacterial inhibition based on the category of inhibition by Davis and Stout of ethanol extract from pangi (Pangium edule Reinw. ex Blume) leaf performed by disc diffusion Kirby and Bauer method againts Staphylococcus aureus, Escherichia coli and Pseudomonos aeruginosa bacterias. The result showed that the ethanol extract from pangi (Pangium edule Reinw. Ex Blume) leaf has medium inhibitory strength at concentrations of 4%, 6% and 8%.Keywords: Pangium edule Reinw. ex Blume leaf, Antibacterial, Disc diffusion method  ABSTRAKSalah satu bahan alam yang sering digunakan sebagai tumbuhan obat adalah tumbuhan pangi (Pangium edule Reinw. ex Blume). Bagian dari tumbuhan pangi yang sering digunakan adalah bagian daun, dimana telah diketahui mempunyai aktivitas antibakteri. Masyarakat Sulawesi Utara secara empiris menggunakan tumbuhan ini sebagai bahan makanan serta untuk menyembuhkan berbagai penyakit seperti mengobati penyakit gatal-gatal pada kulit yang disebabkan oleh bakteri yang terdapat pada kulit. Penelitian ini bertujuan untuk mengetahui kekuatan daya hambat antibakteri berdasarkan kategori penggolongan daya hambat oleh Davis dan Stout dari ekstrak etanol daun Pangium edule Reinw. ex. Blume dengan metode difusi agar (difusi disk Kirby dan Bauer) terhadap bakteri uji Staphyloccocus aureus, Escherichia coli dan Pseudomonas aeruginosa. Hasil penelitian didapati bahwa ekstrak etanol daun Pangium edule Reinw ex. Blume mempunyai kekuatan daya hambat kategori sedang pada konsentrasi 4%, 6% dan 8%.Kata kunci: Daun Pangium edule Reinw. ex Blume, Antibakteri, Metode difusi disk

scholarly journals UJI AKTIVITAS ANTIMIKROBA EKSTRAK ETANOL ORGANISME LAUT SPONS Ianthella basta TERHADAP BEBERAPA MIKROBA PATOGEN

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 100
Author(s):  
Syifa Sari Katili ◽  
Defny S Wewengkang ◽  
Henki Rotinsulu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Marine Organism ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Paper Disc

ABSTRACTSponges are multicellular metazoa animals belonging to the Porifera phylum, which has a different structure from other metazoans. The purpose of this study was to determine whether ethanol extracts from the marine organism sponge Ianthella basta have antimicrobial activity against several pathogenic microbes Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. The extraction method used is maceration with 96% ethanol solvent. The method used is the Diffusion Method (Disc Diffusion Kirby and Bauer). The antimicrobial activity test uses a 6 mm paper disc with 50 µL absorption per disc. The results of crude ethanol extract of Ianthella basta sponge from all test microbes, namely Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans, were seen to provide the greatest inhibitory activity against Staphylococcus aureus bacteria with an average of inhibitory zone of 7.00 mm categorize as intermediate The results obtained showed that the crude extract of the sponge Ianthella basta has antimicrobial activity because it can inhibit the growth of Staphylococcus aureus, Escherichia coli and Candida albicans microbes even though the inhibition zone is categorized as intermediate. Keywords: Ianthella basta, antimicrobial, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans. ABSTRAKSpons adalah hewan metazoa multiseluler tergolong ke dalam filum Porifera, yang memiliki perbedaan struktur dengan metazoan lainnya. Tujuan penelitian ini ialah untuk mengetahui apakah ekstrak etanol dari organisme laut spons Ianthella basta memiliki aktivitas antimikroba terhadap beberapa mikroba patogen Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa dan Candida albicans. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol 96%. Metode yang digunakan yaitu Metode Difusi (Disc Diffusion Kirby and Bauer).  Pengujian aktivitas antimikroba ini menggunakan kertas cakram (paper disc) berukuran 6 mm dengan daya serap 50 µL tiap cakram. Hasil ekstrak kasar etanol Spons Ianthella basta dari semua mikroba uji yaitu Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa dan Candida albicans, terlihat yang memberikan daya hambat paling besar terdapat pada bakteri Staphylococcus aureus dengan jumlah rata – rata zona hambat yaitu 7,00 mm dengan kategori sedang. Hasil penelitian yang diperoleh menunjukkan bahwa ekstrak kasar dari Spons Ianthella basta memiliki aktivitas antimikroba karena mampu menghambat pertumbuhan mikroba uji Staphylococcus aureus, Escherichia coli dan Candida albicans walaupun dengan zona hambat yang  dikategorikan sedang. Kata Kunci : Ianthella basta, antimikroba, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans.

scholarly journals IN VITRO ANTIBACTERIAL EFFECT OF AQUEOUS EXTRACT OF SIDDHARTAKA SNANA USED IN TREATMENT OF VICARCHIKA (ECZEMA)

2021 ◽  
pp. 94-98
Author(s):  
E.R.H.S.S. Ediriweera ◽  
A.M.H.Y Perera ◽  
R. Senavirathne ◽  
R. Rajapaksha
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Skin Diseases ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Cyperus Rotundus ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Vicarchika is a type of Kushta Roga (skin disease). It can be correlated with eczema. Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa are some of the bacteria which cause skin diseases. Siddhartaka Snana is a herbal bath that is described in Charaka Samhita as a treatment for Kushta Roga. Siddhartaka Snana is a herbal preparation which consists ten herbs namely; Cyperus rotundus, Catunaregum spinosa, Phyllanthus emblica, Terminalia chebula, Terminalia bellarica, Cassia fistula, Pongamia pinnata, Holarrhena antidysentrica, Alastonia scholaris and Coscinum fenestratum. Antibacterial effect of Siddhartaka Snana against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa that are responsible for skin diseases was studied using disc diffusion method and well diffusion methods. In disc diffusion method, no inhibition zone was observed with any of the tested bacteria. However, an inhibition zone of 9.67mm was observed against Staphylococcus aureus in well diffusion method with Siddhartaka Snana. Minimum Inhibitory Concentration (MIC) of Siddhartaka Snana against Staphylococcus aureus was 0.9225 mg/1ml. Ingredients of Siddhartaka Snana contain tannins, phenols and flavonoids, all of which possess antibacterial properties. Antibacterial effect of Siddhartaka Snana may have been exhibited due to actions of these phytochemicals. It was concluded that Siddhartaka Snana has an antibacterial effect against Staphylococcus aureus.

scholarly journals Uji Aktivitas Antimikroba Ekstrak Jamur Lingzhi (Ganoderma lucidum) Menggunakan Pelarut Etanol 96% Terhadap Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 41-44
Author(s):  
Prasetyo Handrianto
Keyword(s):  
Staphylococcus Aureus ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Traditional Medicines ◽  
Paper Disc

ABSTRAKStaphylococcus aureus merupakan bakteri yang relatif sering dijumpai pada manusia. Beberapa infeksi yang disebabkan oleh Staphylococcus aureus antara lain, bisul, infeksi luka dan pneumonia. Salah satu obattradisional yang dapat digunakan untuk mengobati infeksi yang disebabkan oleh Staphylococcus aureus adalah jamur lingzhi (Ganoderma lucidum). Penelitian dilakukan dengan 5 konsentrasi ekstrak etanol jamur lingzhi, yaitu kontrol negatif (0 µg/ml), 20 µg/ml, 40 µg/ml, 60 µg/ml, 80 µg/ml dan 100 µg/ml dan dilakukan 6 kali pengulangan. Metode yang digunakan dalam uji aktivitas antimikroba yaitu metode difusi kertas cakram. Data zona hambat dianalisis dengan uji Anova One-Way. Hasil penelitian menunjukkan bahwa ekstrak etanol jamurlingzhi (Ganoderma lucidum) dengan konsentrasi 100 µg/ml memiliki rata-rata daya hambat tertinggi yaitu sebesar 18,0 mm (sangat aktif). Dapat disimpulkan bahwa semakin besar konsentrasi ekstrak jamur lingzhi (Ganoderma lucidum), maka akan berpengaruh besar pula terhadap zona hambat bakteri Staphylococcus aureus.Keyword : Antibakteri, Ganoderma lucidum, Staphylococcus aureus, Etanol.ABSTRACTStaphylococcus aureus is a bacteria that can cause infections , such as boils , wound infections and pneumonia. One of the traditional medicines that can be used to treat these infections are fungal lingzhi (Ganodermalucidum). The study was conducted with five concentrations of negative control (0 µg/ml), 20 µg/ml, 40 µg/ml, 60 µg/ml, 80 µg/ml, and 100 µg/ml with six repeats. The method used in the test of antimicrobial activity is paper disc diffusion method. Data of inhibition zone were analyzed by Anova-One Way test. The results showed that the ethanol extract of lingzhi mushroom (Ganoderma lucidum) at concentration 100 µg/ml has highest average of inhibitory regions as big as 18,0 mm. It can be concluded that the greater the concentration of extract of lingzhi mushroom (Ganoderma lucidum) , it will affect the greater the inhibition zone against Staphylococcus aureus.Keyword : Antibakteri, Ganoderma lucidum, Staphylococcus aureus, Etanol.

scholarly journals Antimicrobial Properties of 6-Bromoeugenol and Eugenol

2016 ◽  
Vol 53 ◽  
pp. 57-64
Author(s):  
Radia Mahboub ◽  
Faiza Memmou
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Disc Diffusion Method ◽  
Modest Activity

We have studied the antimicrobial properties of 6-bromoeugenol and eugenol by three strains:Pseudomonas aeruginosa(S1),Escherichia coli(S2) andStaphylococcus aureus(S3). We have determined the minimum inhibitory concentration (MIC) for a range of concentrations using the disc diffusion method. We note that all samples present an antimicrobial activity toward the tested bacterial strains at different concentrations (1, 0.5 and 0.25 mg/ml). The 6-bromoeugenol gives modest activity with (S1) and (S3). Eugenol reacts positively with thePseudomonas aeruginosa(S1) at all concentrations and with theEscherichiacoli(S2) at 0.5 mg/ml. We remark that thePseudomonas aeruginosa(S1) is the more sensitive strain thanEscherichiacoli(S2) andStaphylococcus aureus(S3). We have estimated the activity coefficient which has confirmed the antimicrobial activity of the different samples. So, 6-bromoeugenol has shown his efficiency as antimicrobial agent.

scholarly journals EVALUATION OF EFFECTIVENESS OF MORINGA'S LEAVES AGAINST Escherichia coli USING DISC DIFFUSION METHOD

2021 ◽  
Vol 7 (1) ◽  
pp. 41-46
Author(s):  
Erin Dhillon ◽  
Margaretia Yenni Rotua ◽  
Adrian Khu ◽  
Ade Sinaga
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Properties ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Control Group ◽  
P Value ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Control Group Design ◽  
Significant Difference

The UPTD Puskesmas annual report book in Medan Tembung District reported that the number of diarrhea sufferers had increased every year. Moringa plants have many benefits, both leaves, flowers, stems, fruit blossoms, and seeds. The moringa leaves contain various phytochemicals, viz. alkaloids, flavonoids, phenols, steroids, triterpenoids, and tannins, which have antimicrobial properties. This study aimed to determine the antimicrobial effect of the ethanol extract of Moringa leaves against Escherichia coli. This study was an experimental study with a posttest only control group design model. The method used to evaluate ethanol extract's anti-bacterial effect from Moringa leaves was the disc diffusion method. Moringa leaf extract was obtained by maceration method with a ratio of Simplicia powder, and ethanol solvent was 1:10. The ethanol extract was then concentrated by rotary evaporator and diluted using DMSO into five different concentrations viz. 70%, 75%, 85%, 90%, and 95%. All data were analyzed by the One Way Anova test and expressed as Mean ± SD. This study showed that all treatment groups in this study had a significant difference in the zone of inhibition; it was shown by the P-value <0.05. Only 70% of concentrations from Serial concentration of extract were significant differences against the positive control. Overall, it can be concluded that the ethanol extract of moringa leaves is effective as an anti-bacteria against Escherichia coli, especially at concentrations > 75%.

scholarly journals Perbandingan Aktivitas Antibakteri Ekstrak Bunga dan Daun Beluntas (Pluchea indica (L.) less.) Terhadap Bakteri Staphylococcus aureus

2021 ◽  
Vol 1 ◽  
pp. 547-553
Author(s):  
Hida Ilyana ◽  
Dwi Bagus Pambudi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Extraction Method ◽  
Research Method ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Clear Zone ◽  
Disc Diffusion Method

AbstractChemical constituents contained in the beluntas plant, including alkaloids, essential oils, flavonoids, phenols and tannins have the ability to inhibit the growth of Staphylococcus aureus bacteria. This research uses maceration extraction method. Thick extracts of flowers and leaves were used with a series of extract concentrations. The purpose of this study was to determine differences in the effectiveness of the ethanol extract of the leaves and flowers of beluntas against the growth of Staphylococcus aureus bacteria. The research method used in this study is the disc diffusion method. Data was collected by measuring the diameter of the clear zone formed in each treatment. Extract concentrations used 5%, 7% and 9%. The extracts of flowers and leaves of beluntas (Pluchea indica (L.) Less) have antibacterial activity with a larger zone of inhibition in extracts of leaves of beluntas compared to extracts of leaves of beluntas.Keywords: beluntas : flowers; leaves; extract; antibacterial AbstrakKandungan kimia yang terdapat di dalam tanaman beluntas, diantaranya alkaloid, minyak atsiri, flavonoid, fenol dan tanin memiliki kemampuan untuk menghambat pertumbuhan bakteri Staphylococcus aureus.Penelitian ini menggunakan metode ekstraksi maserasi.Ekstrak kental bunga dan daundigunakan dengan seri konsentrasi ekstrak. Tujuan dari penelitian ini yaitu mengetahui perbedaan efektivitas aktivitas ekstrak etanol daun dan bunga beluntas terhadap pertumbuhan bakteri Staphylococcus aureus. Metode penelitian yang digunakan dalam penelitian ini yaitu metode difusi cakram disk. Pengumpulan data dilakukan dengan cara mengukur diameter zona bening yang terbentuk pada setiap perlakuan. Konsentrasi ekstrak digunakan 5%, 7% dan 9%.Ekstrak bunga dan daun beluntas (Pluchea indica (L.)Less) memiliki aktivitas sebagai anti bakteri dengan zona hambat yang lebih besar pada ekstrak daun beluntas dibandingkan dengan ekstrak daun beluntas.Kata kunci: Beluntas; bunga; daun; ekstrak; antibakteri

scholarly journals STUDI POTENSI TANAMAN TEBU IRENG (Saccharum officinarum L.) SEBAGAI ANTIOKSIDAN DAN ANTIMIKROBA

2021 ◽  
Vol 20 (1) ◽  
pp. 57-67
Author(s):  
Putri Sri Andila ◽  
I Putu Agus Hendra Wibawa ◽  
I Nyoman Lugrayasa ◽  
Wawan Sujarwo
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Free Radical ◽  
Salmonella Typhimurium ◽  
Saccharum Officinarum ◽  
Free Radical Scavenger ◽  
Diffusion Method ◽  
Radical Scavenger ◽  
Disc Diffusion ◽  
Disc Diffusion Method

Tebu ireng (Saccharum officinarum L.) merupakan jenis tebu lokal yang memiliki ciri khusus yaitu warna batangnya yang hitam.Secara tradisional tebu ireng dimanfaatkan sebagai obat penyakit diabetes.Selain dapat dimanfaatkan secara tradisional sebagai obat diabetes, diyakini tebu ireng masih banyak menyimpan manfaat lain yang belum banyak diketahui. Studi ini dilakukan untuk mengetahui efektivitas ekstrak tebu ireng sebagai antioksidan dan antimikroba, ditinjau dari beberapa bagian tanamannya. Proses ekstraksi menggunakan metode maserasi dengan pelarut methanol, uji aktivitas antioksidan dilakukan dengan metode DPPH free radical scavenger menggunakan spektrofotometer UV-Vis. Pengujian aktivitas antimikroba dilakukan dengan menggunakan metode difusi agar (Kirby-Bauer disc diffusion method). Hasil studi menunjukkan bahwa tebu ireng memiliki kemampuan sebagai antiokidan.Tingkat kepekatan warna dari tebu ireng berkorelasi dengan aktifitas antiokidannya.Ekstrak dari keseluruhan bagian tanaman tebu ireng efektif untuk menghambat pertumbuhan bakteri Staphylococcus aureus, Salmonella typhimurium, Pseudomonas aeruginosa dan Streptococcus mutans, namun tidak efektif menghambat pertumbuhan jamur Candida albicans.Tebu ireng mengandung pigmen Antosianinserta kaya akan serat pangan .

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN DADAP SEREP (Erythrina lithosperma Miq.) TERHADAP PERTUMBUHAN Escherichia coli

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Asep Abdul Rahman ◽  
Ridwan Firmansyah ◽  
Lulu Setyabudi
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Abdominal Pain ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Activity Test ◽  
Paper Disc

Dadap Serep (Erythrina lithosperma Miq.) is one of the plants containing secondary metabolites in the form of alkaloids, flavonoids and tannins. Empirically dadap serep can be used to treat fever, abdominal pain, breast milk, prevent miscarriage, inflammation and cough. The aim of this research is to know the activity of ethanol extract in dadap serep (Erythrina lithosperma Miq.) in inhibiting growth of Escherichia coli ATCC 8939. The dadap serep leaves were extracted by maceration method using 96% ethanol solvent. The leaf thickness extract of dadap serep with concentration of 20%, 40%, 60% and 80% tested its antibacterial activity against growth of Escherichia coli ATCC 8939 using paper disc diffusion method. The result of concentration of leaf extract of dadap serep, obtained rendemen as much as 23,53%. Then the result of antibacterial activity test showed that leaf extract of dadap serep with concentration of 20% had no inhibition to growth of Escherichia coli ATCC 8939, while at concentration extract 40%, 60%, and 80% had inhibitory to growth of Escherichia coli ATCC 8939 with category weak resistor response.Keywords: Dadap serep (Erythrina lithosperma Miq.), Antibacterial activity, Escherichia coli ATCC 8939

scholarly journals UJI SITOTOKSIK DAN AKTIVITAS ANTIBAKTERI EKSTRAK DAUN PANDAN LAUT (Pandanus Odorifer) TERHADAP BAKTERI Staphylococcus aureus

Alotrop ◽  
2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Shinta Puspasari ◽  
Nurhamidah Nurhamidah ◽  
Hermansyah Amir
Keyword(s):  
Staphylococcus Aureus ◽  
Secondary Metabolite ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Toxic Level ◽  
Cytotoxic Test ◽  
Zone Diameter ◽  
Lc50 Value

This study aims to determine the activity of marine pandanus extract(Pandanus odorifer) as cytotoxic and antibacterial to Staphylococcus aureus bacteria. This research begins with phytochemical test conducted to find out the secondary metabolite compounds contained in the leaves of P. odorifer. Cytotoxic testing was performed using BSLT method. An antibacterial test was performed against the activity in inhibiting the growth of S. aureus bacteria using a modified disc diffusion method. The results of phytochemical tests showed that ethanol extract of pandanus leaves contain saponin, flavonoids, tannin and terpenoid compounds. The results of cytotoxic test of sea pandan have a very strong toxic level with LC50 value of 4.3557 ppm.The result of measurement of the highest inhibiting zone diameter was obtained at the concentration of 5x104 ppm is 6 mm with the power of inhibitory that is medium. Based on the results of this study can be concluded that the leaves of sea pandan are cytotoxic and have activity as antibacterial

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