scholarly journals IDENTIFIKASI FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN DENGAN METODE 1.1-diphenyl-2-picrylhydrazyl (DPPH), DAN TOKSISITAS DENGAN METODE Brine Shrimp Lethality Test (BSLT) DARI EKSTRAK ETANOL TANGKAI BUAH PINANG YAKI (Areca vestiaria Giseke)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 619
Author(s):  
Glorya Karundeng ◽  
Herny E. I. Simbala ◽  
Imam Jayanto
Keyword(s):  
Brine Shrimp ◽  
Ethanol Extract ◽  
Areca Nut ◽  
Brine Shrimp Lethality ◽  
Plant Toxicity ◽  
Phytochemical Content ◽  
North Sulawesi ◽  
The People ◽  
Ic50 Value ◽  
Very High

ABSTRACT Pinang Yaki (Areca vestiaria Giseke) which is a kind of wild palm, is a multi-functional plant. The people of North Sulawesi empirically use this plant to cure various diseases. In a previous study that said leaves, seeds and even the seed skin of areca nut have very high antioxidant content. Because of the large amount of antioxidants contained in Pinang Yaki plants, the study wanted to examine the compound content of the stalks of this plant. This study aims to determine the phytochemical content, ability of antioxidant activity, and plant toxicity, so that later it can be used as medicine. The results of this study indicate that the ethanol extract of areca fruit stalk has flavonoids, saponins, triterpenoids which have the potential as antioxidants and have a strong Inhibition Concetration 50 (IC50) value, ie, 16.52 μg / mL based on the 1.1-diphenyl-2-picrylhydrazyl method (DPPH), and the level of toxicity, namely the value of Lethality Concetration 50 (LC50) of 602 μg / mL which was carried out by the Brine Shrimp Lethality Test (BSLT) method. Keywords: Areca vestiaria Giseke, Antioxidants, Toxicity, DPPH, BSLT ABSTRAK Pinang Yaki (Areca vestiaria Giseke) yang merupakan sejenis palem liar, merupakan tanaman yang multi fungsi. Masyarakat Sulawesi Utara secara empiris menggunakan tanaman ini untuk menyembuhkan berbagai macam penyakit. Pada penelitian yang sebelumnya mengatakan bahwa daun, biji bahkan kulit biji dari Pinang Yaki mempunyai kandungan antioksidan yang sangat tinggi. Oleh karena banyaknya kandungan antioksidan yang terkandung dalam tanaman Pinang Yaki maka peneliti ingin meneliti kandungan senyawa dari tangkai buah tanaman ini. Penelitian ini bertujuan untuk mengetahui kandungan fitokimia, kemampuan aktivitas antioksidan, dan toksisitas tanaman, agar nantinya bisa digunakan sebagai obat. Hasil penelitian ini menunjukkan bahwa ekstrak etanol tangkai buah Pinang Yaki memiliki kandungan flavonoid, saponin, triterpenoid yang berpotensi sebagai antioksidan dan memiliki nilai Inhibisi Concetration 50 (IC50) yang sangat kuat yakni, 16.52 μg/mL berdasarkan pengujian dengan metode 1.1-diphenyl-2-picrylhydrazyl (DPPH), dan  kadar toksisitas yakni nilai Lethality Concetration 50 (LC50) sebesar 602 μg/mL yang dilakukan dengan metode pengujian Brine Shrimp Lethality Test (BSLT). Kata kunci: Pinang Yaki, Antioksidan, Toksisitas, DPPH, BSLT

scholarly journals SKRINING FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN, DAN TOKSISITAS DARI EKSTRAK DAUN KERSEN (Muntingia calabura L.) DENGAN METODE 1.1-diphenyl-2-picrylhydrazyl (DPPH) dan Brine Shrimp Lethality Test (BSLT)

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 315
Author(s):  
Selin Widjaya ◽  
Widdhi Bodhi ◽  
Adithya Yudistira
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Brine Shrimp Lethality ◽  
Phytochemical Content ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Dpph Method ◽  
Muntingia Calabura

ABSTRACTKersen (Muntingia calabura L.) is a plant that has begun to be eliminated and was rarely used because it is often considered to have no economic value and lack of knowledge about its utilization, whereas kersen plants contain flavonoids, saponins, and tannins which were have high benefit for health. The content of metabolites is affected by soil nutrient elements and difference place of growth. This study aims to determine the potential of kersen leaves grown in North Minahasa based on phytochemical content, ability of antioxidant activity, and toxicity. Kersen leaves were extracted using sequential maceration method with n-hexane, ethyl acetate, and ethanol as solvents. Phytochemical Screening using several reagents which tailored to the type of phytochemical test. 1.1-diphenyl-2-picrylhydrazyl (DPPH) method is used to evaluate antioxidant activity, and Brine Shrimp Lethality Test (BSLT) method is used to evaluate toxicity. The result of this study indicate that the n-hexane extract contains phenols, flavonoids, and tannins, with IC50 value 12.54 μg/mL, and LC50 value 881 μg/mL. Ethyl acetate extract contains phenols, flavonoids, tannins, and saponins, with IC50 value 61.3 μg/mL, and LC50 value 1758 μg/mL. Ethanol extract has phenol, flavonoid, tannin, saponin, and terpenoid content, with IC50 value 9.01 μg/mL, and LC50 value 106 μg/mL. Keywords : Kersen leaves, Antioxidant, Toxicity, IC50, LC50 ABSTRAKKersen (Muntingia calabura L.) merupakan tanaman yang sudah mulai tersingkirkan dan jarang dimanfaatkan karena sering dianggap tidak punya nilai ekonomis dan kurangnya pengetahuan tentang pemanfaatannya, padahal tanaman kersen memiliki kandungan flavonoid, saponin, dan tanin yang bermanfaat tinggi untuk kesehatan. Kandungan senyawa metabolit dipengaruhi oleh unsur hara tanah dan perbedaan tempat tumbuh. Penelitian ini bertujuan untuk mengetahui potensi dari daun kersen yang tumbuh di Minahasa Utara berdasarkan kandungan fitokimia, kemampuan aktivitas antioksidan, dan toksisitasnya. Ekstrak daun kersen diekstraksi dengan metode maserasi sekuensial menggunakan pelarut n-heksan, etil asetat, dan etanol.  Skrining fitokimia menggunakan beberapa reagen yang disesuaikan dengan jenis uji fitokimia. Metode 1.1-diphenyl-2-picrylhydrazyl (DPPH) digunakan untuk mengevaluasi aktivitas antioksidan, dan metode Brine Shrimp Lethality Test (BSLT) digunakan untuk mengevaluasi toksisitas. Hasil penelitian ini menunjukkan bahwa ekstrak n-heksan memiliki kandungan fenol, flavonoid, dan tanin, nilai IC50 12,54 μg/mL, dan nilai LC50 881 μg/mL. Ekstrak etil asetat memiliki kandungan fenol, flavonoid, tanin, dan saponin, nilai IC50 61,3 μg/mL, dan nilai LC50 1758 μg/mL. Ekstrak etanol memiliki kandungan fenol, flavonoid, tanin, saponin, dan terpenoid, nilai IC50 9,01 μg/mL, dan nilai LC50 106 μg/mL. Kata kunci : Daun Kersen, Antioksidan, Toksisitas, IC50, LC50

scholarly journals Uji Toksisitas Ekstrak Daun Benalu Langsat (Dendrophthoe petandra (L) Miq) dengan Metode Brine Shrimp Lethality Test (BSLT) LC50

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 157
Author(s):  
Jhon Smith Wongkar ◽  
Max R. J. Runtuwene ◽  
Jemmy Abidjulu
Keyword(s):  
Toxic Effect ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
North Sulawesi ◽  
Experimental Organism

Telah dilakukan penelitian tentang toksisitas daun benalu langsat (Dendrophthoe petandra) dengan metode Brine Shrimp Letality Test (BSLT). Metode ini menggunakan etanol sebagai pelarut untuk mengekstrak daun benalu langsat dan udang Artemia salina sebagai organisme uji serta dilakukan pada suhu 25-30 0C. Sampel benalu langsat yang di uji didapatkan dari Desa Kali Selatan Kecamatan Pineleng Kabupaten Minahasa Sulawesi utara. Penentuan toksisitas dari ekstrak etanol daun benalu langsat menggunakan konsentrasi 500, 100, 20, 10, dan 1 ppm. Hasil penelitian menunjukkan bahwa semakin besar konsentrasi yang diberikan maka sifat toksisitas ekstrak daun benalu langsat juga akan semakin besar dengan nilai LC50 sebesar 0,561.A research on toxicity of benalu langsat (Dendrophthoe petandra) leaf by using Brine Shrimp Letality Test (BSLT) method has been done. This method using ethanol as solvent to extract benalu langsat leaf and shrimp Artemia salina as experimental organism at 25-30 oC. Benalu langsat leaf was obtained from village South Kali district Pineleng, Minahasa North Sulawesi. Toxicity examination used 500, 100, 20, 10, dan 1 ppm ethanol extract of benalu langsat leaf. The results showed that the higher the concentration of the extract, the higher the toxic effect shown with LC50 value of 0.561.

Evaluation of α-amylase Inhibition and Cytotoxic Activities of the Arachis hypogaea and Cinnamomum tamala

2020 ◽  
Vol 16 ◽  
Author(s):  
Deedarul Hyder Sani ◽  
Ali Newaz Munna ◽  
Mohammad Salim ◽  
Md. Jahangir Alam ◽  
Md. Jahangir Alam
Keyword(s):  
Brine Shrimp ◽  
Ethanol Extract ◽  
Acetone Extract ◽  
Cytotoxic Activities ◽  
Inhibition Activity ◽  
Inhibition Assay ◽  
Peanut Seed ◽  
Lc50 Value ◽  
Cinnamomum Tamala ◽  
Ic50 Value

Background: Diabetes mellitus is the most occurring non-communicable disease resulting in a high blood glucose level. There has been an immense interest in the development of alternative medicines for diabetes treatment, specifically screening functional foods for phytochemicals with the capability of delaying or preventing glucose absorption through digestive enzymes (e.g. α-amylase) inhibition. So, the development of α-amylase inhibitors derived from natural food products is an alternative way to prevent diabetes mellitus Objective: In this study, organic solvent extracts of the Arachis hypogaea (Peanut) and Cinnamomum tamala (Indian bay leaf /Tejpata) were used to investigate their potential α-amylase inhibition and cytotoxic activities through α-amylase inhibition assay and brine shrimp lethality bioassay respectively Method: The α-amylase inhibition assay was performed using the 3,5-dinitrosalicylic acid method for different concentrations of plant extracts. The optical density (OD) of the solutions were measured to determine the inhibition activity at 540 nm using a spectrophotometer. The cytotoxicity of the plant extracts was measured using brine shrimp (Artemia salina) lethality bioassay Results: Among the different organic solvent extracts, peanut seed ethanol extract showed the highest α-amylase inhibition activity (67.68±8.67%) at 1.25 μg/mL concentration with an IC50 value of 0.61 μg/mL which is very close to standard α-amylase inhibitor Acarbose (72.34±4.23%) with an IC50 value of 0.32 μg/mL while acetone extract of Indian bay leaf exhibited the lowest inhibition activity (47.75±1.63%) with an IC50 value of 1.42 μg/mL at the same concentration. Besides, the maximum cytotoxic activity was found in acetone extract of peanut shell with an LC50 value of 57.87 μg/mL whereas ethanol extract of peanut seed showed the lowest cytotoxicity with an LC50 value of 413.90 μg/mL Conclusion: The result of the present work clearly indicates the potentiality of peanut seed ethanol extract to be used in the management of hyperglycemia as it significantly inhibits α-amylase activity while showing less cytotoxic activities

scholarly journals KAJIAN TOKSISITAS DARI TUBUH BUAH Ganoderma lucidum DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST)

2014 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Ross Nurul Rohmah ◽  
Nuniek Ina Ratnaningtyas ◽  
Ari Asnani
Keyword(s):  
Toxic Effect ◽  
Fruiting Body ◽  
Brine Shrimp ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
One Stage ◽  
Body Extract

Ganoderma lucidum is polyporus fungi from Basidiomycetes which can be used as traditional medicines. Toxicity test with Brine Shrimp Lethality Test (BST) method using Artemia salina was conducted to find out toxic effect of G.lucidum. An extract would have toxic effect if the LC50 < 1000 µg/ml. The aims of this experiment were to know about the toxicity level from G.lucidum with Brine Shrimp Lethality Test (BST) method and determined the concentration of fruiting body extract of G.lucidum which had the best toxicity effect in LC50. There were two extraction methods used in this experiment, first one stage extraction and then multilevel extraction with hexane, ethyl acetate, and ethanol. Each solvent was made in 1000 ppm, 500 ppm, 250 ppm, and 125 ppm. This process was repeated for three times. This experiment used G.lucidum extract which was tested to A.salina and secunder compound metabolit test from the most toxic G. lucidum was done with phytochemical analysis. The result indicated that fruiting body extract from G.lucidum could kill A.salina because all extract was positively contained alkaloid and terpenoid but negatively contained flavonoid. Fruiting body extract from G.lucidum which had lowest LC50 wass ethyl asetat extract with one stage extraction in concentration 53,70 ppm and highest LC50 was ethanol extract with multilevel extraction in concentration 501,18 ppm.

scholarly journals Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract

Elkawnie ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 67
Author(s):  
Robby Gus Mahardika ◽  
Occa Roanisca ◽  
Fajar Indah Puspita Sari
Keyword(s):  
Toxicity Test ◽  
Brine Shrimp ◽  
Leaf Extract ◽  
Positive Control ◽  
Acetone Extract ◽  
Antidiabetic Activity ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Ft Ir

Abstract : This study aims to determine the antidiabetic activity and toxicity of the acetone extract of Tristaniopsis merguensis Griff leaf. The antidiabetic test was the α-glucosidase inhibition method, while the toxicity test used the Brine Shrimp Lethality Test (BSLT) method. The acetone extract possessed antidiabetic activity with an IC50 value of 8.83 ± 0.31 (μg/mL). This value is not much different from the positive control of quercetin which has an IC50 value of 6.04 ± 0.14 (μg/mL). The characteristics of the FT-IR spectrum of acetone extract showed that Tristaniopsis merguensis leaf has the groups Ar-OH (phenolic), -OH (hydroxyl), C=O (ketone) and C=C (aromatic). Based on the toxicity test, the Tristaniopsis merguensis leaf acetone extract has an LC50 value of 959.25 ppm which means that the acetone extract is toxic. Therefore, the acetone extract of Tristaniopsis merguensis might be the potential agent of antidiabetic.Abstrak : Penelitian ini bertujuan untuk mengetahui aktivitas antidiabetes dan toksisitas dari ekstrak aseton daun Tristaniopsis merguensis Griff. Uji aktivitas antidiabetes ditentukan berdasarkan metode inhibisi enzim α-glucosidase, sedangkan toksisitas ditentukan berdasarkan metode Brine Shrimp Lethality Test (BSLT). Ekstrak aseton memiliki aktivitas antidiabetes dengan nilai IC50 8,83 ± 0,31 (μg/mL). Nilai ini tidak jauh berbeda dengan kontrol positif quersetin yang memiliki nilai IC50 6,04 ± 0,14 (μg/mL). Karakteristik spektrum FT-IR ekstrak aseton menunjukkan bahwa daun Tristaniopsis merguensis memiliki gugus fungsi Ar-OH (fenolik), -OH (hidroksil), C=O (keton) dan C=C (aromatik). Berdasarkan uji toksisitas, ekstrak aseton daun Tristaniopsis merguensis memiliki nilai LC50 sebesar 959,25 ppm yang berarti bahwa ekstrak aseton bersifat toksik. Oleh karena itu, ekstrak aseton dari Tristaniopsis merguensis berpotensi untuk dijadikan agen antidiabetes.

Antioxidant, Antityrosinase Activity and Toxicity of Alpinia nigra Extracts

2019 ◽  
Vol 819 ◽  
pp. 111-117 ◽  
Author(s):  
Papatsiri Janyapanich ◽  
Chatchanok Kotipan ◽  
Kritsanat Teerachawalwong ◽  
Surawut Watana ◽  
Nopparat Nuntharatanapon
Keyword(s):  
Free Radical ◽  
Brine Shrimp ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Aqueous Extracts ◽  
Mushroom Tyrosinase ◽  
Melanin Production ◽  
Brine Shrimp Lethality ◽  
Alpinia Nigra

Excessive of ultraviolet light causes abnormality of melanin production. Antioxidants and antityrosinase agents are able to reduce hyperpigmentation by interrupting the process of melanin production. The purpose of this study is to examine the antioxidant and antityrosinase activities as well as toxicity of both 80% ethanol and aqueous extracts of Alpinia nigra by DPPH free radical scavenging assay, mushroom tyrosinase assay and brine shrimp lethality bioassay. Alpinia nigra extracts showed positive result on antioxidant and antityrosinase activities. We found that extract of A. nigra’s leaf has the most effective activity of antioxidant and antityrosinase among other parts of this plant. The ethanol and aqueous extracts from the leaf of A. nigra at the concentration of 125 μg/mL showed % inhibition for free radical scavenging as 94.97% and 93.35%, respectively. The IC50 values of antioxidant were 39.83±16.21 and 46.33±15.22 μg/mL, respectively. In addition, ethanol extract of the leaf from A. nigra at the concentration of 1,000 μg/mL produced 92.61% inhibition of mushroom tyrosinase activity, whereas aqueous extract of A. nigra’s leaf at the same concentration produced 74.47% inhibition. The IC50 of antityrosinase activities were 142.81±13.32 and 406.88±66.43 μg/mL for ethanol and aqueous extracts, respectively. Moreover, the brine shrimp lethality bioassay showed that all extracts were non-toxic (LC50 >1,000 μg/mL). In conclusion, the ethanol extract of A. nigra’s leaf may be beneficial and provide the novel and safe source for antioxidant and whitening agent.

scholarly journals Toxicity Test of Ethanol Extract Ant Plant Local Aceh (Mymercodia sp) Method of BSLTLarvae Shrimp Artemia salina Leach

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Frengki F ◽  
Roslizawaty R ◽  
Desi Pertiwi
Keyword(s):  
Crude Extract ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Rotary Evaporator

Toxicity test and phytochemical screening of ant plant ethanol extract were done. The toxicity test was done by employing the Brine Shrimp Lethality Test (BSLT) method. Sample of ant plant taken from the Aceh Besar forest have been extracted with ethanol. The sample was thenconcentrated using a rotary evaporator until a crude extract was obtained. The extract was tested by toxicity to larva Artemia salina L. Thetoxicity test for larvae Artemia salina Leach have been done by employing the Brine Shrimp Lethality Test (BSLT) method at four concentrationlevels, i.e: 250 ppm, 125 ppm, 50 ppm, and 25 ppm. The result of the research showed that ethanol extract of ant plant Aceh had acute toxicityby LC value 61.11 ppm. The extract had identified were fenolik, saponin, steroid and terpenoid.____________________________________________________________________________________________________________________Key words: rine Shrimp Lethality Test, LC5050, Ant Plant, Aceh, Bphytochemical Screening 

scholarly journals ANALISIS SENYAWA METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK ETANOL BATANG TANAMAN PATAH TULANG (Euphorbia tirucalli L.) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2014 ◽  
Vol 14 (2) ◽  
pp. 106 ◽  
Author(s):  
Grace S. Baud ◽  
Meiske S. Sangi ◽  
Harry S.J. Koleangan
Keyword(s):  
Secondary Metabolite ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Euphorbia Tirucalli ◽  
Ethanol Extracts

ABSTRAK Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman Patah tulang (Euphorbia tirucalli L.). Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia yaitu senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak segar dan kering batang tanaman Patah tulang. Penentuan toksisitas ekstrak etanol batang tanaman Patah tulang menggunakan metode Brine Shrimp Lethality Test (BSLT). Uji toksisitas digunakan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh positif flavonoid, tanin dan steroid sedangkan alkaloid, saponin dan triterpenoid negatif. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan metode probit menggunakan perangkat lunak SPSS 20.0 (untuk sistem operasi Windows). Hasil uji toksisitas ekstrak etanol batang Patah tulang menunjukkan bahwa ekstrak segar maupun kering bersifat sitotoksik dengan nilai LC50 (7,994ppm) untuk ekstrak etanol batang segar  dan LC50 (9,940ppm) untuk ekstrak etanol batang kering. Kata kunci : Euphorbia tirucalli L., Senyawa metabolit sekunder, Toksisitas, Artemia salina Leach, LC50.   ANALYSIS OF SECONDARY METABOLITE COMPOUNDS AND TOXICITY TEST OF  STEM  PLANT ETHANOL EXTRACTS OF  PATAH TULANG (Euphorbia tirucalli L.) BY BRINE SHRIMP LETHALITY TEST’S METHOD (BSLT) ABSTRACT A research on the analysis of secondary metabolite compounds contained in the stem of  patah tulang (Euphorbia tirucalli L.) and its toxicity had been done. The analysis was performed through phytochemical screening of alkaloids, flavonoids, triterpenoids, steroids, saponins and tannins on extracts of fresh and dried stem of the plant. Toxicity of ethanol extract of the stem was determined using Brine Shrimp Lethality Test (BSLT) method with Artemia salina Leach as bioindicator. Phytochemical screening showed positive results for flavonoids, tannins, and steroids and negative one for alkaloids, saponins, and triterpenoids. Toxicity data were obtained from Lethality Concentration 50 (LC50) calculation trhrough probit analysis using SPSS 20.0 software (for Windows operating systems). Toxicity test showed that ethanol extracts of the fresh and dried stem were cytotoxic with LC50 value of 7.994 ppm and 9.940 ppm, respectively. Keywords: Euphorbia tirucalli L., Secondary metabolite compounds, Toxicity, Artemia salina Leach, LC50.

scholarly journals Isolation of Quercetin-3-O-beta-d-glucopyranoside from the Leaves of Azadirachta Indica and Antimicrobial and Cytotoxic screening of the Crude Extracts

2012 ◽  
Vol 60 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Monirul Islam ◽  
MD Al-Amin ◽  
M Mahboob Ali Siddiqi ◽  
Shakila Akter ◽  
Mohammad Majedul Haque ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Azadirachta Indica ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Soluble Fraction ◽  
Ethanol Extract ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Test Organisms ◽  
Soluble Fractions

Quercetin-3-O-beta-D-glucopyranoside was isolated from the ethylacetate soluble fraction of the ethanol extract of the fresh leaves of Azadirachta indica (Family: Meliaceae). The crude extract of hexane, ethylacetate and butanol soluble fractions of this ethanol extract were subjected to antimicrobial screening and brine shrimp lethality bioassay. The ethylacetate crude extract exhibited moderate antimicrobial activity against most of the test organisms and also showed significant cytotoxicity having LC50 0.61 µg/ml.DOI: http://dx.doi.org/10.3329/dujs.v60i1.10328Dhaka Univ. J. Sci. 60(1): 11-14, 2012 (January)

scholarly journals A Cytotoxic Activity of Tahongai (Kleinhovia hospita Linn.) Leaves Extracts Using Brine Shrimp Lethality Test

2019 ◽  
Vol 4 (3) ◽  
pp. 60
Author(s):  
Indah Solihah ◽  
Herlina Herlina ◽  
Riana Sari Puspita Rasyid ◽  
Tri Suciati ◽  
Khairunnisa Khairunnisa
Keyword(s):  
Statistical Analysis ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Ethanol Extracts

Tahongai (Kleinhovia hospita Linn.) leaves had been known contain alkaloids, flavonoids, saponins, steroids, and tannins. At Komering, South Sumatera tribe, tahongai leaves had been known to treat tumor, cancer, polyps, acne, and dysmenorrhea. The study of cytotoxic activity of tahongai bark and stem were done. This study aims to determine the cytotoxic activity of tahongai leaves extracts using BSLT method. Tahongai leaves were extracted using gradual maceration with n-hexane, ethyl acetate, and ethanol 96%. Each extract was tested cytotoxic activity towards Artemia salina L. larvae. The yield of n-hexane, ethyl acetate, and ethanol extracts are 2,686%, 7,033%, and 7,933% respectively. Ethanol extract of tahongai leaves had the best cytotoxic activity with lethality value 76,667% at 500ppm. Statistical analysis with two way ANOVA showed extract and concentration had a significant (p<0,05) effect on larvae lethality percentage.

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