scholarly journals UJI AKTIVITAS ANTIMIKROBA DARI JAMUR LAUT YANG BERASOSIASI DENGAN ORGANISME LAUT SPONS Callyspongia aerizusa

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 1007
Author(s):  
Fransisca Macpal ◽  
Adithya Yudistira ◽  
Defny S. Wewengkang
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Coral Reef ◽  
Ethyl Acetate ◽  
Marine Fungi ◽  
North Sulawesi ◽  
Liquid Fractionation ◽  
Bioactive Potential

ABSTRACTSponge is the lowest level hollow animal without a spine. Sponges are one of the components of coral reef biota that have bioactive potential that has not been widely used. This study aims to determine whether there is antimicrobial activity of marine fungi associated with sponge Callyspongia aerizusa obtained from Southeast Minahasa Regency, North Sulawesi, against the growth of microbes such as Staphylococcus aureus, Escherichia coli and Candida albicans. Samples were obtained then extracted by maceration using acetone and fractionated using liquid-liquid fractionation with ethyl acetate solvents. The results of this study indicate that the extract from the fungus associated with the Callyspongia aerizusa sponge has activity against Escherichia coli and Candida albicans. Keywords: Antimicrobials, Callyspongia aerizusa, Candida albicans, Escherichia coli, Marine fungi, Staphylococcus aureus.  ABSTRAKSponge (spons laut) adalah hewan berongga tanpa tulang belakang yang paling rendah tingkatannya. Spons merupakan salah satu komponen biota penyusun terumbu karang yang mempunyai potensi bioaktif yang belum banyak dimanfaatkan. Penelitian ini bertujuan untuk mengetahui apakah terdapat aktivitas antimikroba dari jamur laut yang berasosiasi dengan spons Callyspongia aerizusa yang di peroleh dari Kabupaten Minahasa Tenggara, Sulawesi Utara, terhadap pertumbuhan mikroba seperti Staphylococcus aureus, Escherichia coli dan Candida albicans. Sampel diperoleh kemudian diekstraksi secara maserasi dengan aseton dan difraksinasi dengan menggunakan fraksinasi cair-cair menggunakan pelarut etil asetat. Hasil penelitian ini menunjukkan bahwa ekstrak dari jamur yang berasosiasi dengan spons Callyspongia aerizusa memiliki aktivitas terhadap bakteri Escherichia coli dan bakteri Candida albicans. Kata kunci:  Antimikroba, Callyspongia aerizusa, Candida albicans Escherichia  coli,.Jamur laut,  Staphylococcus aureus

scholarly journals AKTIVITAS ANTIMIKROBA EKSTRAK ETIL ASETAT JAMUR LAUT YANG DIISOLASI DARI ORGANISME LAUT SPONS Phylospongioa lamellosa YANG DIAMBIL DARI PERAIRAN DESA TUMBAK, KECAMATAN PUSOMAEN, KABUPATEN MINAHASA TENGGARA TERHADAP MIKROBA Staphylococcus aureus, Escherichia coli, dan Candida albicans

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 851
Author(s):  
Priscila Irene Tumiwa ◽  
Adithya Yudistira ◽  
Defny S. Wewengkang
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Coral Reef ◽  
Marine Invertebrates ◽  
Symbiotic Relationships ◽  
Paper Disk ◽  
Coral Reef Ecosystems ◽  
Fungal Inoculation

ABSTRACT Sponges are multi-cell marine invertebrates whose tissue and organ functions are very simple which live in coral reef ecosystems. Sponges are known to produce bioactive compounds because of their symbiotic relationships with microorganisms so that they have the potential to be developed in the field of medicine including as an antimicrobial. This study aims to determine whether the fungus associated with Phyllospongia lamellose sponge taken from the waters of, Southeast Minahasa Regency has antimicrobial activity. This research includes sampling, isolation and fungal inoculation, fermentation, extraction with acetone and then fractionated with ethyl acetate solvent,dried until it gets crude extracts and is carried out as well as antimicrobial testing against Staphylococcus aureus, Escherichia coli, and Candida albicans. Antimicrobial activity was obtained from inhibitory zones formed around the paper disk against the test microbes. From the results of the study concluded that the fungus associated with the Phyllospongia lamellose sponge has antimicrobial activity against the bacteria Staphylococcus aureus, Escherichia coli, and Candida albicans. Keywords: Phyllospongia lamellose sponge, Antimicrobial, Stapylococcus aureu, Escherichia coli, Candida albicans. ABSTRAK Spons merupakan invertebrata laut multi sel yang fungsi jaringan dan organnya sangat sederhana yang hidup pada ekosistem terumbu karang. Spons diketahui menghasilkan senyawa bioaktif karena adanya hubungan simbiotik dengan mikro organisme sehingga berpotensi untuk dikembangkan dalam bidang pengobatan diantaranya sebagi antimikroba. Penelitian ini bertujuan untuk mengetahui apakah jamur yang berasosiasi dengan spons Phyllospongia lamellose yang diambil dari perairan Desa Tumbak, Kecamatan Posumaen, Kabupaten Minahasa Tenggara memiliki aktivitas antimikroba. Penelitian ini meliputi kegiatan pengambilan sampel spons Phyllospongia lamellose, isolasi dan inokulasi jamur yang berasosiasi dengan spons, fermentasi, ektraksi dengan aseton kemudian difraksinasi dengan pelarut etil asetat, dikeringkan hingga mendapat ekstrak kasar dan dilakukan serta pengujian antimikroba terhadap bakteri Staphylococcus aureus, Escherichia coli, dan jamur Candida albicans. Aktivitas antimikroba didapatkan dari zona hambat yang terbentuk disekitaran cakram kertas terhadap mikroba uji. Dari hasil penelitian disimpulkan bahwa jamur yang berasosiasi dengan spons Phyllospongia lamellose memiliki aktivitas antimikroba terhadap bakteri Staphylococcus aureus, Escherichia coli, dan jamur Candida albicans. Kata Kunci : Spons Phyllospongia lamellose, Antimikroba, Stapylococcus aureu, Escherichia coli, Candida albicans.

UJI AKTIVITAS ANTIMIKROBA DARI JAMUR LAUT YANG BERASOSIASI DENGAN ALGA Halimeda opuntia

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 41
Author(s):  
Ridwan Sukardi Djakatara ◽  
Defny S Wewengkang ◽  
Henki Rotinsulu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Marine Fungi ◽  
Marine Ecosystem ◽  
Antimicrobial Compounds ◽  
E Coli ◽  
The World

ABSTRACT Algae is a place of life for various microorganisms symbiosis with it, one of which is fungi. Marine fungi are known to have an important contribution to the marine ecosystem. Many types of marine fungi that have been isolated and are known to produce a number of antimicrobial compounds were become a new choice in the world of health. This study aims to determine the antimicrobial activity of isolates fungi which associated with Halimeda opuntia algae collected from the Bay of Manado against Staphylococcus aureus, Escherichia coli, and Candida albicans. The results showed extracts of fungi that were associated with algae Halimeda opuntia did not possesed antimicrobial activity against S. aureus and E.coli and C. albicans. Keywords: Antimicrobials, marine fungi, Halimeda opuntia ABSTRAK Alga merupakan tempat hidup berbagai mikroorganisme yang bersimbiosis dengannya, salah satunya ialah jamur. Jamur laut diketahui memiliki kontribusi yang penting bagi ekosistem laut. Banyak jenis jamur laut yang telah diisolasi dan diketahui menghasilkan sejumlah senyawa antimikroba yang kini menjadi pilihan baru di dunia kesehatan. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antimikroba dari isolat jamur yang berasosiasi dengan alga Halimeda opuntia yang diperoleh dari Teluk Manado terhadap bakteri  Staphylococcus aureus, Escherichia coli, dan jamur Candida albicans. Hasil penelitian menunjukkan ekstrak dari jamur yang berasosiasi dengan alga Halimeda opuntia tidak memiliki aktivitas antimikroba terhadap bakteri S. aureus dan E. coli dan jamur C. albicans. Kata kunci : Antimikroba , jamur laut , Halimeda opuntia

scholarly journals AKTIVITAS ANTIMIKROBA EKSTRAK ETIL ASETAT JAMUR LAUT YANG DIISOLASI DARI KARANG LUNAK Sarcophyton sp. DARI PERAIRAN DESA TUMBAK KECAMATAN PUSOMAEN

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 142
Author(s):  
Englin Meiva Paat ◽  
Defny S Wewengkang ◽  
Henki Rotinsulu
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Coral Reef ◽  
Inhibitory Activity ◽  
Soft Coral ◽  
Gram Negative Bacteria ◽  
Gram Positive Bacteria ◽  
North Sulawesi ◽  
Coral Reef Ecosystems

ABSTRACT Soft coral is one type of Coelenterata that lives at sea, namely coral reef waters. Soft Coral acts as one of the constituent animals of coral reef ecosystems and is the largest supplier of growth compounds, such as carbonate compounds in which 50% of the bioactive compounds found in these invertebrates are toxic. This study aims to determine the antimicrobial activity of marine fungi associated with soft coral Sarcophyton sp., which was obtained from Tumbak Village, Posumaen Sub-district, Southeast Minahasa Regency, North Sulawesi. The antimicrobial testing uses diffusion methods to determine the inhibitory activity against Staphylococcus aureus (Gram positive bacteria), Escherichia coli (Gram negative bacteria) and Candida albicans (fungi). The results showed that the antimicrobial activity of fungal extracts isolated from soft coral Sarcophyton sp., against bacteria, Escherechia coli, and Candida albicans by measuring the inhibitory activity is the formation of clear zones which categorize as medium, whereas for Staphylococcus aureus bacteria do not have antimicrobial activity. Keywords: Antimicrobial, Soft Coral (Sarcophyton sp.), Staphylococcus aureus, Eschrichia coli,                      Candida albicans ABSTRAK Karang Lunak merupakan salah satu jenis Coelenterata yang hidupnya dilaut yaitu perairan terumbu karang. Karang Lunak berperan sebagai salah satu hewan penyusun ekosistem terumbu karang dan pemasok senyawa pertumbuhan terbesar yaitu senyawa karbonat yang dimana sebanyak 50 % senyawa bioaktif yang terdapat pada invetebrata ini bersifat toksik. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antimikroba dari jamur laut yang berasosisasi dengan karang lunak Sarcophyton sp., yang diperoleh dari desa Tumbak Kecamatan Posumaen Minahasa Tenggara Sulawesi Utara. Pengujian daya antimikroba menggunakan metode difusi untuk mengetahui aktivitas penghambatan yang diuji terhadap Staphylococcus aureus (bakteri Gram positif), Eschrichia coli (bakteri Gram negatif) dan Candida albicans (jamur). Hasil penelitian menunjukkan bahwa adanya aktivitas antimikroba ekstrak jamur yang diisolasi dari karang lunak Sarcophyton sp., terhadap bakteri, Escherechia coli, dan jamur Candida albicans dengan pengukuran daya hambat yaitu terbentuknya zona bening yang ada dalam kategori sedang, sedangkan untuk bakteri Staphylococcus aureus tidak memiliki aktivitas antimikroba. Kata Kunci: Antimikroba, Karang Lunak (Sarcophyton sp.), Staphylococcus aureus, Eschrichia                          coli, Candida albicans

Screening and antimicrobial activity of actinomycetes isolated from the rhizosphere of Clitoria ternatea

2016 ◽  
Vol 1 (01) ◽  
Author(s):  
Vemavarapu Bhaskara Rao ◽  
Kandlagunta Guru Prasad ◽  
Krishna Naragani ◽  
Vijayalakshmi Muvva
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Calcium Carbonate ◽  
Rhizosphere Soil ◽  
Soil Samples ◽  
Antimicrobial Spectrum ◽  
Clitoria Ternatea ◽  
Primary Screening

The air dried rhizosphere soil samples pretreated with calcium carbonate was employed for the isolation of actinomycete strains. Serial dilution plate technique was used for the isolation of actinomycetes. A total of 20 actinomycete strains designated as BS1-BS20 were isolated from the rhizosphere of medicinal plant Clitoria ternatea. All the 20 strains were subjected to primary screening for antimicrobial activity. Among the 20 strains screened, 10 strains exhibited high antimicrobial spectrum against Staphylococcus aureus, Escherichia coli and Candida albicans.

The In vitro Evaluation of the Antimicrobial Activity of Quercus robur L. Methanolic and Aqueous Leaves’ Extracts, from the Algerian High Plateaus Against some Uropathogenic Microbial Strains

2019 ◽  
Vol 18 (5) ◽  
pp. 262-274
Author(s):  
E. Benyagoub ◽  
N. Nabbou ◽  
S. Boukhalkhel ◽  
I. Dehini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Fusidic Acid ◽  
Quercus Robur ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Microbial Strains

The medicinal value of the plants is due to their chemical components that bring a definite physiological action on the human body to prevent the diseases. In this work, we investigated the antimicrobial activity of leaves’ extracts of Quercus robur L., collected from the Algerian upper highlands, on ten bacterial strains and one fungal strain known to be pathogenic. First, we performed a qualitative phytochemical analysis, and second, antimicrobial activity tests performed by agar diffusion method (disc and well) with the determination of MIC by broth macro-dilution method. Given the results, it appears that obtained macerates of Quercus robur L. were rich in bioactive phytoconstituents such as alkaloids, anthraquinones, saponins, tannins, and other components. The yield of aqueous and methanolic macerates of leaves was 8.5 ± 1.41 and 22.4 ± 4.36%, respectively. The bacterial resistance was relatively important to several antibiotics, namely, ampicillin, amoxicillin + clavulanic acid for strains of Escherichia coli and Salmonella sp. However, Staphylococcus aureus strains were resistant to fusidic acid, penicillin, and oxacillin; while Enterococcus faecalis was resistant to fusidic acid, penicillin, oxacillin, and ticarcillin. The antibacterial activity of the macerates toward tested microbial strains showed that the aqueous and methanolic macerates of the leaves were proportional to the tested concentration and active not only against Gram-positive and Gram-negative bacteria but also on the fungal species Candida albicans. The estimated MIC for Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus was in the order of 10 mg/mL, which seems more effective than toward Salmonella sp., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans which were in the order of 30 mg/mL. These preliminary results confirm that the part of the studied plant had a very good antimicrobial activity that was proportional to the serial concentrations of the tested extracts.

scholarly journals Antimicrobial Effect of Plasma-Activated Tap Water on Staphylococcus aureus, Escherichia coli, and Candida albicans

Water ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1480
Author(s):  
William Chiappim ◽  
Aline da Graça Sampaio ◽  
Felipe Miranda ◽  
Mariana Fraga ◽  
Gilberto Petraconi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Tap Water ◽  
Antimicrobial Effect ◽  
Gliding Arc ◽  
Oxidation Reduction ◽  
Treatment Conditions ◽  
Oxidation Reduction Potential

In this study, the potential antimicrobial activity of plasma-activated tap water (PAW) was evaluated against Staphylococcus aureus, Escherichia coli, and Candida albicans. For this, PAW was prepared in a gliding arc plasma system using two treatment conditions: stagnant water and water stirring by a magnetic stirrer, called moving water. Subsequently, their oxidation-reduction potential (ORP), pH, electrical conductivity (σ), and total dissolved solids (TDS) were monitored in different areas of the sample divided according to the depth of the beaker. It was observed that PAW obtained in dynamic conditions showed a more uniform acidity among the evaluated areas with pH 3.53 and ORP of 215 mV. Finally, standardized suspensions of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 10799), and Candida albicans (SC 5314) were treated with PAW, and the reduction of viable cells determined the antimicrobial effect. Our results indicate that the tap water, activated by plasma treatment using gliding arc, is an excellent inactivation agent in the case of Staphylococcus aureus and Escherichia coli. On the other hand, no significant antimicrobial activity was achieved for Candida albicans.

scholarly journals UJI DAYA HAMBAT EKSTRAK DAN FRAKSI SPONS Aaptos Aaptos TERHADAP PERTUMBUHAN Escherichia coli, Staphylococcus aureus, dan Candida albicans

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 168
Author(s):  
Sitti N Tunggali ◽  
Herny E. I. Simbala ◽  
Henki Rotinsulu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Microbial Growth ◽  
Ethanol Extract ◽  
Chloroform Fraction ◽  
Marine Biota ◽  
Disc Diffusion ◽  
Average Value

ABSTRACT Sponge Aaptos aaptos is a marine biota that has great potential, which can be applied, in the pharmaceutical field because of the presence of large compounds in inhibiting microbial growth. This study aims to determine the inhibitory activity of extracts and fractions of sponge Aaptos aaptos on microbial growth of Escherichia coli, Staphylococcus aureus, and Candida albicans. The samples were extracted by maceration with 96 % ethanol and fractioned with n-hexane, choloroform and methanol. Testing is done using the Disc Diffusion Agar method. Crude ethanol extract and fraction of sponge Aaptos aaptos showed the greatest antimicrobial activity against Staphylococcus aureus and categorized as strong, with an average value of 20.32 mm for ethanol extract with strong categories, chloroform fraction 13,28 mm with medium category and methanol fractions 18,48 mm strong category. Keyword: Aaptos aaptos, antimicrobial activity, Escherichia coli, Staphylococcus aureus, Candida albicans.  ABSTRAK Spons Aaptos aaptos merupakan biota laut yang memiliki potensi sebagai antimikroba yang dapat diterapkan di bidang farmasi dengan kandungan senyawa yang besar dalam menghambat pertumbuhan mikroba. Penelitian ini bertujuan untuk mengetahui aktivitas daya hambat dari ekstrak dan fraksi spons Aaptos aaptos terhadap pertumbuhan mikroba Escherichia coli, Staphylococcus aureus, dan Candida albicans. Sampel diekstraksi secara maserasi dengan etanol dan difraksinasi dengan pelarut n–heksan, kloroform dan metanol. Pengujian dilakukan dengan menggunakan metode Disc Diffusion Agar. Ekstrak kasar etanol dan fraksi dari Spons Aaptos aaptos menunjukkan aktivitas antimikroba paling besar terhadap Staphylococcus aureus dan dikategorikan kuat, dengan nilai rata – rata 20,32 mm untuk ekstrak etanol dengan kategori kuat, fraksi kloroform 13,28 mm, kategori sedang dan fraksi metanol 18,48 mm kategori kuat.Kata Kunci : Aaptos aaptos, aktivitas antimikroba, Escherichia coli, Staphylococcus aureus, Candida albicans

scholarly journals BIOAKTIVITAS JAMUR Aspergillus flavus YANG BERSIMBION DENGAN ASCIDIAN Eudistoma sp.

2020 ◽  
Vol 8 (2) ◽  
pp. 11
Author(s):  
Alfa Mongi ◽  
Deiske A Sumilat ◽  
Fitje Losung ◽  
Remmy E P Mangindaan ◽  
Rosita A Lintang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Aspergillus Flavus ◽  
Marine Invertebrate ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Antibacterial And Antifungal ◽  
Ethyl Acetate Extracts

Ascidian is a marine invertebrate that produce bioactive compounds such as antibacterial and antifungal. The purpose of this study was to obtain symbiotic fungi isolates, ethyl acetate extracts from ascidian symbiotic isolates, and to determine the antibacterial and antifungal activities of ethyl acetate extracts of symbiotic isolates using the diffusion method (disc diffusion Kirby and Bauer) against Escherichia coli, Staphylococcus aureus bacteria and against the Candida albicans fungus. The results obtained 1 ascidian species which was cultured to obtain pure isolates, the pure isolates were tested molecularly and the results showed that the isolate was Aspergillus flavus, then the isolates were grown on rice medium and extracted. The results of the fungal extract showed the presence of antibacterial and antifungal activity against Escherichia coli by 10 mm, Staphylococcus aureus by 19 mm and against the Candida albicans by 13.5 mm. Keywords : Ascidian, Eudistoma sp., Aspergillus flavus, Antibacterial, Antifungal, Symbiotic.                                                  AbstrakAscidian merupakan avertebrata laut yang dapat menghasilkan senyawa bioaktif yang seperti antibakteri dan antijamur. Tujuan dari penelitian ini yaitu mendapatkan isolat jamur simbion dari ascidian, mendapatkan ekstrak etil asetat dari isolat jamur simbion ascidian, dan menguji aktivitas antibakteri dan antijamur ekstrak etil asetat isolat jamur simbion dengan metode difusi agar (disc diffusion Kirby and Bauer) terhadap bakteri Escherichia coli, Staphylococcus aureus dan terhadap jamur Candida albicsans. Hasil penelitian didapatkan 1 jenis ascidian yang kemudian dikultur untuk mendapatkan isolat murni, isolat murni diuji secara molekuler dan didapati hasil bahwa isolat tersebut merupakan jamur Aspergillus flavus. Isolat tersebut kemudian ditumbuhkan pada media nasi dan diekstrak. Hasil ekstrak jamur tersebut menunjukkan adanya aktivitas antibakteri dan antijamur terhadap bakteri Escherichia coli sebesar 10 mm, Staphylococcus aureus sebesar 19 mm dan terhadap jamur Candida albicsans 13,5 mm. Kata kunci : Ascidian, Eudistoma sp., Aspergillus flavus, Antibakteri, Antijamur, Simbion.

scholarly journals Evaluation du potentiel antimicrobien et de la toxicité des extraits de Jatropha multifida Linn, (Euphorbiaceae)

2020 ◽  
Vol 151 ◽  
pp. 15550-15558
Author(s):  
Amégninou Agban ◽  
Yao Hoekou ◽  
Passimna Pissang ◽  
Tchadjobo Tchacondo ◽  
Komlan Batawila
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aqueous Extract ◽  
E Coli ◽  
Toxicological Studies ◽  
Jatropha Multifida

Objectif : L’objectif de ce travail était d’évaluer in vitro l’activité antimicrobienne des extraits de feuilles et tige de Jatropha multifida sur la croissance de Candida albicans, Escherichia coli et Staphylococcus aureus, puis d’évaluer in vivo la toxicité de cette plante. Méthodologie et résultats : Les méthodes de diffusion en milieu gélosé et de microdilution en milieu liquide ont été utilisées pour évaluer l’effet antimicrobien. Une étude en subaigüe était réalisée afin d’explorer les effets toxiques de l’extrait aqueux des feuilles. Les résultats des tests antimicrobiens montrent une activité des extraits de feuilles et tige de J. multifida sur la croissance des souches utilisées avec des diamètres de zones d’inhibition allant de 8 à 25 mm et des concentrations minimales inhibitrices (CMI) variant de 0,039 mg/mL à 1,25 mg/mL à l’exception des souches de E. coli qui sont résistantes aux extraits de la tige. L’administration en subaigüe de l’extrait aqueux des feuilles de J. multifida à la dose de 600 mg/kg entraîne une perte significative de poids chez les souris. Conclusion et applications des résultats : Les extraits aqueux, éthanolique et hydroéthanolique des feuilles et tige de J. multifida possèdent d’activité antimicrobienne et pourraient être utilisés dans le traitement des Candidoses à C. albicans et des infections à S. aureus. Mais l’essai de toxicité subaigüe montre que l’extrait aqueux de la plante serait toxique. Des études toxicologiques approfondies restent donc nécessaires sur ces extraits afin de mieux élucider leur inocuité. Mots-clés : Jatropha multifida, extraits de feuilles et de tige, activités antifongique et antibactérienne, toxicité. Agban et al., J. Appl. Biosci. 2020 Evaluation du potentiel antimicrobien et de la toxicité des extraits de Jatropha multifida Linn, (Euphorbiaceae) 15551 Evaluation of antimicrobial potential and toxicity of Jatropha multifida Linn, (Euphorbiaceae) extracts ABSTRACT Objective: The objective of this study was to evaluate in vitro the antimicrobial activity of leaves and stem of Jatropha multifida extracts against Candida albicans, Escherichia coli and Staphylococcus aureus, and then to evaluate in vivo the toxicity of this plant. Methodology and Results: The agar well-diffusion and the NCCLS broth microdilution methods were used to assess the antimicrobial effect. A subacute study was carried out to explore the toxic effects of the aqueous extract of the leaves. The results of the antimicrobial tests show an activity of the extracts of leaves and stems of J. multifida on the growth of the strains used with diameters of inhibitory zones ranging from 8 to 25 mm and minimum inhibitory concentrations (MIC) varying from 0.039 mg/mL to 1.25 mg/mL exception E. coli strains which are resistant to extracts from the stem. Subacute administration of the aqueous extract of the leaves of J. multifida at a dose of 600 mg/kg leads to a significant loss of weight in the mice. Conclusion and application of findings : The aqueous, ethanolic and hydroethanolic extracts of the leaves and stem of J. multifida have antimicrobial activity and could be used in the treatment of Candidiasis and bacterial infections due respectively to C. albicans and S. aureus. But the subacute toxicity test shows that the aqueous extract of the plant would be toxic. Extensive toxicological studies therefore remain necessary on these extracts in order to better elucidate their safety. Keywords: Jatropha multifida extracts of leaves and stem, antifungal and antibacterial activities, toxicity

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