scholarly journals Development of Single-Chain Variable Fragment (ScFv) antibody against COVID-19 by phage display as a possible tool to diagnostic and treatment

2021 ◽  
Author(s):  
Alexandre Figueiredo ◽  
Thiago Chaves ◽  
Fernando Conte ◽  
Rodrigo Silva ◽  
Milena Carvalho ◽  
...  
Keyword(s):  
Phage Display ◽  
Scfv Antibody ◽  
Single Chain Variable Fragment ◽  
Single Chain

scholarly journals Isolation of a novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library

2019 ◽  
Author(s):  
Shirafkan Kordi ◽  
Mohammad Rahmati-Yamchi ◽  
Mehdi Asghari Vostakolaei ◽  
Ali Etemadie ◽  
Abolfazl Barzegari ◽  
...  
Keyword(s):  
Phage Display ◽  
Growth Factor Receptor ◽  
Phage Display Library ◽  
Vital Role ◽  
Scfv Antibody ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Phage Display Technology ◽  
Display Technology ◽  
Domain 3

Vascular endothelial growth factor receptor 2 (VEGFR-2) is known as one of the important antigens playing a vital role in angiogenesis. In this study, phage display technology (PDT) was used to produce a single-chain variable fragment (scFv) antibody against a region of the domain 3 in VEGFR-2 called kinase insert domain receptor 3 (KDR3). After designing the KDR3 peptide and biopanning, a colony was chosen for scFv antibody expression. Following expression and purification; western blotting, dot blotting and immunofluorescence (IF) were used to evaluate the antibody function. Surface plasmon resonance (SPR) was also employed to measure affinity of produced antibody. Once a colony was selected and transferred to the expression host, the scFv antibody was expressed in the expected range of 28 kDa. Using a designed chromatography column, antibody purification was found to be about 95%. In this study, a novel scFv with the capability of binding to KDR3 was isolated and purified and its intracellular function was investigated and verified.

scholarly journals Construction of a Single-Chain Variable-Fragment Antibody against the Superantigen Staphylococcal Enterotoxin B

2010 ◽  
Vol 76 (24) ◽  
pp. 8184-8191 ◽  
Author(s):  
Pawan Kumar Singh ◽  
Ranu Agrawal ◽  
Dev Vrat Kamboj ◽  
Garima Gupta ◽  
M. Boopathi ◽  
...  
Keyword(s):  
Phage Display ◽  
Staphylococcal Enterotoxin ◽  
Scfv Antibody ◽  
Affinity Constant ◽  
Enzyme Linked Immunosorbent Assay ◽  
Staphylococcal Enterotoxin B ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
High Affinity ◽  
Enterotoxin B

ABSTRACT Staphylococcal food poisoning (SFP) is one of the most prevalent causes of food-borne illness throughout the world. SFP is caused by 21 different types of staphylococcal enterotoxins produced by Staphylococcus aureus. Among these, staphylococcal enterotoxin B (SEB) is the most potent toxin and is a listed biological warfare (BW) agent. Therefore, development of immunological reagents for detection of SEB is of the utmost importance. High-affinity and specific monoclonal antibodies are being used for detection of SEB, but hybridoma clones tend to lose their antibody-secreting ability over time. This problem can be overcome by the use of recombinant antibodies produced in a bacterial system. In the present investigation, genes from a hybridoma clone encoding monoclonal antibody against SEB were immortalized using antibody phage display technology. A murine phage display library containing single-chain variable-fragment (ScFv) antibody genes was constructed in a pCANTAB 5E phagemid vector. Phage particles displaying ScFv were rescued by reinfection of helper phage followed by four rounds of biopanning for selection of SEB binding ScFv antibody fragments by using phage enzyme-linked immunosorbent assay (ELISA). Soluble SEB-ScFv antibodies were characterized from one of the clones showing high affinity for SEB. The anti-SEB ScFv antibody was highly specific, and its affinity constant was 3.16 nM as determined by surface plasmon resonance (SPR). These results demonstrate that the recombinant antibody constructed by immortalizing the antibody genes from a hybridoma clone is useful for immunodetection of SEB.

Increasing Expression of an Anti-Picloram Single-Chain Variable Fragment (ScFv) Antibody and Resistance to Picloram in Transgenic Tobacco (Nicotiana tabacum)

2005 ◽  
Vol 53 (17) ◽  
pp. 6683-6690 ◽  
Author(s):  
Fernando Olea-Popelka ◽  
Michael D. McLean ◽  
Jonathan Horsman ◽  
Kurt Almquist ◽  
Jim E. Brandle ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Transgenic Tobacco ◽  
Scfv Antibody ◽  
Single Chain Variable Fragment ◽  
Single Chain

Single chain variable fragment antibodies against Shiga toxins isolated from a human antibody phage display library

Vaccine ◽  
2011 ◽  
Vol 29 (33) ◽  
pp. 5340-5346 ◽  
Author(s):  
Paola Neri ◽  
Naoko Shigemori ◽  
Susumu Hamada-Tsutsumi ◽  
Kentaro Tsukamoto ◽  
Hideyuki Arimitsu ◽  
...  
Keyword(s):  
Phage Display ◽  
Phage Display Library ◽  
Human Antibody ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Shiga Toxins ◽  
Antibody Phage ◽  
Antibody Phage Display
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