Increasing Expression of an Anti-Picloram Single-Chain Variable Fragment (ScFv) Antibody and Resistance to Picloram in Transgenic Tobacco (Nicotiana tabacum)

2005 ◽  
Vol 53 (17) ◽  
pp. 6683-6690 ◽  
Author(s):  
Fernando Olea-Popelka ◽  
Michael D. McLean ◽  
Jonathan Horsman ◽  
Kurt Almquist ◽  
Jim E. Brandle ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Transgenic Tobacco ◽  
Scfv Antibody ◽  
Single Chain Variable Fragment ◽  
Single Chain

Transgenic Tobacco Plants Expressing a Dimeric Single-chain Variable Fragment (scFv) Antibody Against Salmonella enterica Serotype Paratyphi B

2005 ◽  
Vol 14 (5) ◽  
pp. 785-792 ◽  
Author(s):  
Shokouh Makvandi-Nejad ◽  
Michael D. McLean ◽  
Tomoko Hirama ◽  
Kurt C. Almquist ◽  
C. Roger MacKenzie ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Salmonella Enterica ◽  
Scfv Antibody ◽  
Transgenic Tobacco Plants ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Tobacco Plants ◽  
Paratyphi B

scholarly journals NICOTIANA TABACUM AS A POTENTIAL PLATFORM FOR THE PRODUCTION OF RECOMBINANT ANTI-TOXOPLASMA SINGLE-CHAIN VARIABLE FRAGMENT (scFv) ANTIBODY

2020 ◽  
Vol 39 (2) ◽  
pp. 1-26
Author(s):  
Fatin Iffah Rasyiqah Mohamad Zoolkefli ◽  
Pei See Go ◽  
Boon Chin Tan ◽  
Nur Ardiyana Rejab ◽  
Rofina Yasmin Othman ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Scfv Antibody ◽  
Single Chain Variable Fragment ◽  
Single Chain

scholarly journals Engineering of a Single-Chain Variable-Fragment (scFv) Antibody Specific for the Stolbur Phytoplasma (Mollicute) and Its Expression in Escherichia coli and Tobacco Plants

1998 ◽  
Vol 64 (11) ◽  
pp. 4566-4572 ◽  
Author(s):  
Fabrice Le Gall ◽  
Joseph-Marie Bové ◽  
Monique Garnier
Keyword(s):  
Escherichia Coli ◽  
Transgenic Tobacco ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Cells ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Tobacco Plants ◽  
Challenge Inoculation ◽  
Scfv Gene ◽  
Stolbur Phytoplasma

From a hybridoma cell line (2A10) producing an immunoglobulin G1 directed against the major membrane protein of the stolbur phytoplasma, we have engineered scFv (single-chain variable-fragment) antibodies from the variable heavy (VH) and light (VL) domains of the immunoglobulin. The scFv gene was cloned and expressed inEscherichia coli. The expressed protein of 30 kDa could be recovered from the periplasmic fraction of the bacterial cells and was shown to be fully functional toward its phytoplasmal antigen, since enzyme-linked immunosorbent assay or immunofluorescence (IF) detection of the stolbur phytoplasma antigen by the scFv was identical to that of the native immunoglobulin. The scFv gene was then cloned in plasmid pBG-dAb-BIN of Agrobacterium tumefaciens to transform tobacco plants. The transformed plants were screened by PCR and Northern blotting for the presence and expression of the transgene, respectively, and by IF for expression of the scFv. One transgenic tobacco line, 1A6, was selected for challenge inoculation with the stolbur phytoplasma. When grafted on a stolbur phytoplasma-infected tobacco rootstock, the transgenic tobacco shoots grew free of symptoms and flowered after 2 months, while normal tobacco shoots showed severe stolbur symptoms during the same period and eventually died.

Characterization of a single-chain variable fragment (scFv) antibody directed against the human asialoglycoprotein receptor

2006 ◽  
Vol 44 (2) ◽  
pp. 65 ◽  
Author(s):  
Guanxin Shen ◽  
Limin Cao ◽  
Yinchang Zhu ◽  
Weiyu Wang ◽  
Xiaorong Zhao ◽  
...  
Keyword(s):  
Asialoglycoprotein Receptor ◽  
Scfv Antibody ◽  
Single Chain Variable Fragment ◽  
Single Chain

scholarly journals Developing and characterization of single chain variable fragment (scFv) antibody against frizzled 7 (Fzd7) receptor

Bioengineered ◽  
2016 ◽  
Vol 8 (5) ◽  
pp. 501-510 ◽  
Author(s):  
Hamid Nickho ◽  
Vahid Younesi ◽  
Leili Aghebati-Maleki ◽  
Morteza Motallebnezhad ◽  
Jafar Majidi Zolbanin ◽  
...  
Keyword(s):  
Scfv Antibody ◽  
Single Chain Variable Fragment ◽  
Single Chain
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