Improve potato seeds in potato seed farming: practical account

2019 ◽  
pp. 10-17
Author(s):  
Natalia Gaitova ◽  
Boris Anisimox ◽  
Sergey Zhevora ◽  
Valentina Boyko ◽  
Natalia Gaitova ◽  
...  
Keyword(s):  
Viral Infection ◽  
Source Material ◽  
Potato Seed ◽  
High Quality ◽  
Distinctive Features ◽  
Main Variety ◽  
New Perspective ◽  
Basic Seed

The technological process of growing potato seed material begins with the acquisition of the source material and its accelerated reproduction to the required volumes. To include new perspective varieties and hybrids in seed programs, they must be released from a viral infection. Exemption from viruses is a responsible, time-consuming and costly process that requires professionalism, but in the absence of this stage, promising varieties cannot participate in the implementation of seed programs. Only in vitro, a material thoroughly assessed with regard to the presence of pathogens, the varietal typicality and severity of the main variety distinctive features can ensure the high quality of seed potatoes in basic seed production.

scholarly journals Production of pre-basic potato seed by polyvinyl chloride PVC: articulate gutters hydroponic system

2009 ◽  
Vol 52 (5) ◽  
pp. 1107-1114 ◽  
Author(s):  
Jonny Everson Scherwinski-Pereira ◽  
Carlos Alberto Barbosa Medeiros ◽  
Gerson Renan de Luces Fortes ◽  
Arione da Silva Pereira
Keyword(s):  
Crop Yields ◽  
Potato Production ◽  
Potato Seed ◽  
Seed Potato Production ◽  
Hydroponic System ◽  
Hydroponic Systems ◽  
Productive Systems ◽  
Basic Seed

The development of more efficient and productive systems for pre-basic seed potato production would improve the quality of the propagative material used by the potato growers, directly affecting the crop yields. A two-year experiment was carried out to evaluate the potato pre-basic seed production by two types of hydroponic systems (fibrocement tiles and articulated PVC gutters), two cultivars (`Baronesa` and `Eliza`) and two types of propagative material (plants coming from in vitro culture and minitubers). The PVC gutters system was highly efficient. When using minitubers, this system reached multiplication rates up to 74 tubers per plant. Minitubers were more productive than in vitro plants, independent of cultivar and hydroponic system utilized.

Properties of 4H-SiC by Sublimation Close Space Technique

1999 ◽  
Vol 572 ◽  
Author(s):  
S. Nishino ◽  
K. Matsumoto ◽  
Y. Chen ◽  
Y. Nishio
Keyword(s):  
Schottky Diode ◽  
Source Material ◽  
Epitaxial Layers ◽  
Power Devices ◽  
High Quality ◽  
Substrate Quality ◽  
Space Technique ◽  
P Type ◽  
Type Conduction

ABSTRACTSiC is suitable for power devices but high quality SiC epitaxial layers having a high breakdown voltage are needed and thick epilayer is indispensable. In this study, CST method (Close Space Technique) was used to rapidly grow thick epitaxial layers. Source material used was 3C-SiC polycrystalline plate of high purity while 4H-SiC(0001) crystals inclined 8° off toward <1120> was used for the substrate. Quality of the epilayer was influenced significantly by pressure during growth and polarity of the substrate. A p-type conduction was obtained by changing the size of p-type source material. The carrier concentration of epilayer decreased when a lower pressure was employed. Schottky diode was also fabricated.

scholarly journals Percepatan Penyediaan Benih Sumber Kedelai Unggul Secara In Vitro

Agrikultura ◽  
2017 ◽  
Vol 28 (3) ◽  
Author(s):  
Erni Suminar ◽  
Sumadi Sumadi ◽  
Syariful Mubarok ◽  
Toto Sunarto ◽  
Nita Suswati Endah Rini
Keyword(s):  
Growth Failure ◽  
Culture Method ◽  
Coconut Water ◽  
High Quality ◽  
Seed Technology ◽  
Randomized Design ◽  
Vitamin B5 ◽  
New Varieties

ABSTRACTIn vitro method to fasten availability high quality soybean seedsSoybean is an important crop as a source of food and its demand has increased every year. Several new varieties of soybean have been generated, but the number is still limited. Furthermore, the infestation of pests and infection of diseases have also limited the new soybean varieties production as it increase the risk of growth failure. Therefore, methods to fasten the availability of high quality of soybean seeds need to be developed. One of which can be done through in vitro culture method. The objective of this study was to obtain the best type and the best concentration of cytokinin for the growth of soybean explants in vitro. The experiment was conducted in Seed Technology and Tissue Technology Laboratory, Faculty of Agriculture, Universitas Padjadjaran. The experimental design used was Completely Randomized Design with 13 treatments and four replications. The basic media used were Murashige and Skoog (MS) + Vitamin B5 with addition of BAP (1.0 mg/l, 1.5 mg/l, and 2.0 mg/l), Kinetin (0.5 mg/l, 1.0 mg/l, and 1.5 mg/l), TDZ (0.01 mg/l, 0.1 mg/l, and 1.0 mg/l), and coconut water (10%, 15%, and 20%). The result showed that cytokinin types and concentrations gave different effect to soybean explant growth. The best treatment was demonstrated by BAP at the concentration of 1.5 mg/l as shown by the highest percentage of leaves and the number of shoots. However, it did not affect the percentage of buds produced by explant and the percentage of callus produced by explant.Keyword : BAP, Kinetin, TDZ, Coconut water, Soybean.AbstrakKedelai merupakan komoditas yang memegang peranan penting. Permintaan akan kedelai meningkat setiap tahunnya. Varietas kedelai baru yang bersifat unggul sudah banyak dihasilkan, namun jumlahnya masih terbatas. Tingginya serangan hama dan penyakit saat perbanyakkan benih di lapangan menyebabkan tingginya resiko kegagalan dalam pertumbuhan varietas baru tersebut. Sehingga perlu dilakukan percepatan penyediaan benih sumber varietas unggul di laboratorium secara in vitro. Tujuan penelitian ini adalah untuk mendapatkan jenis dan konsentrasi sitokinin yang terbaik untuk pertumbuhan eksplan kedelai in vitro. Penelitian dilakukan di Laboratorium Kultur Jaringan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan tiga belas perlakuan dan empat ulangan. Media dasar yang digunakan adalah Murashige dan Skoog (MS) + Vitamin B5 dengan penambahan BAP (1,0 mg/l; 1,5 mg/l; 2,0 mg/l), Kinetin (0,5 mg/l; 1,0 mg/l; 1,5 mg/l), TDZ (0,01 mg/l; 0,1 mg/l; 1,0 mg/l), dan air kelapa (10%; 15%; 20%). Hasil percobaan menunjukkan bahwa pemberian jenis dan konsentrasi sitokinin memberikan pengaruh yang berbeda terhadap pertumbuhan eksplan. Perlakuan terbaik diperoleh pada BAP dengankonsentrasi 1,5 mg/l yang ditunjukkan dengan tingginya persentase jumlah daun dan tunas yang terbentuk. Akan tetapi, penambahan sitokinin tersebut tidak memberikan pengaruh terhadap persentase eksplan dalam membentuk tunas dan persentase eksplan dalam membentuk kalus.Kata Kunci : BAP, Kinetin, TDZ, Air Kelapa, Kedelai

scholarly journals Comparative Study of Growth Statistics of Two Species of Paulownia and Optimization of Rooting Methods

2019 ◽  
Vol 6 (1) ◽  
pp. 11-15
Author(s):  
Hari Krishna Saiju ◽  
Abhishesh Bajracharya ◽  
Brishav Rajbahak ◽  
Stuti Ghimire
Keyword(s):  
Growth Rate ◽  
Musical Instruments ◽  
Shoot Length ◽  
Ms Medium ◽  
High Quality ◽  
Paulownia Tomentosa ◽  
Fast Growing ◽  
The Family

Paulownia is a fast-growing woody tree, native to the forests of China. It belongs to the family Scrophulariaceae and is mainly used as a source of wood for furniture and musical instruments. Due to its fast-growing nature and high-quality of wood, there has been growing interest in cultivation and research of Paulownia in Nepal. Growth comparison was performed by measuring shoot length in in vitro condition. Among two species of Paulownia - Paulownia tomentosa (Thunb.) Steud and Paulownia fortuneii (Seem.) Hemsl., the growth rate of P. tomentosa was found to be 0.355 cm/week while that of P. fortuneii was found to be 0.637 cm/week in in-vitro conditions in MS medium supplemented with 0.1 mg/l NAA and 1mg/l BAP. Optimization of rooting methods was also performed, in which, sand rooting was found to be easier and more effective than in-vitro rooting. Dipping the plantlets in 1 mg/l of NAA was found to produce longer and denser roots than lower or higher concentrations during sand rooting.

scholarly journals The source material of soft spring wheat for improving the quality of grain and resistance to lodging

2021 ◽  
Vol 36 ◽  
pp. 01023
Author(s):  
V.V. Novokhatan ◽  
E.V. Zuev ◽  
T.V. Shelomentseva ◽  
T.A. Leonova
Keyword(s):  
Source Material ◽  
Intensive Agriculture ◽  
High Quality ◽  
Wheat Varieties ◽  
Important Indicator ◽  
Fine Grain ◽  
Arid Conditions ◽  
Local Selection ◽  
High Yielding Varieties

The Northern Trans-Urals is a region of intensive agriculture that needs high-yielding varieties that are resistant to lodging and a-biotic environmental factors, forming high-quality grain. In this respect, as a starting material for breeding, Canadian varieties with good technological indicators of grain and Norwegian varieties with high resistance to lodging and well-expressed economic and valuable characteristics are of interest. An important indicator when creating wheat varieties is the resistance to pre-harvest germination of grain on the root. The conjugacy of this feature with the yield is high and is expressed negatively - r = -0.922. Among the studied cultivars, Demonstrant (Norway) and 5603HR (Canada) stand out for their resistance to grain germination in the ear. Norwegian varieties are characterized by multi-grain ear, fine grain and compacted ear. Marker traits of drought resistance – the length of the upper internode and the removal of the ear, are better expressed in the standard of Omskaya 36, varieties of local selection and varietals - Laban and GN 06600 (Norway). In arid conditions, these varieties have a more pronounced yield. The best in this respect is Kazakhstan one - Astana (2.27 t/ha). The intensity was highlighted by the variety - GN 06600 (Norway) - 5.05 t/ha. The revealed conjugate relationships in the studied traits allow to purposefully conduct breeding work.

scholarly journals Ionocidium ‘Cerrado 101’: intergeneric orchid hybrid with high quality of blooming

2017 ◽  
Vol 23 (3) ◽  
pp. 351 ◽  
Author(s):  
Jean Carlos Cardoso
Keyword(s):  
Wild Species ◽  
Female Parent ◽  
Cut Flower ◽  
Vegetative Development ◽  
High Quality ◽  
The World ◽  
Flower Type ◽  
Hybrid Cultivar

Orchids are considered one of the most important potted-flowering in the world. Oncidium genus, as well their hybrids group (OHGs) has used for the flower market as pot or cut flower.  However, some horticultural characteristics require improvements, e.g. the production of precocious cultivars, independent-season blooming or easy flowering induction, high quality and durability of flowers and variations in color of flowers, to compete with Phalaenopsis and Dendrobium orchids, as other flower groups. Aiming this purpose there were used hand-crossing pollination between the Oncidium ‘Sweet Sugar’ and Ionopsis utricularioides, a Brazilian wild species, for evaluate the capacity of crossing and to select progenies with interest of use in floriculture as new cultivar. The seeds obtained from this cross were seeded on in vitro conditions, followed by acclimatization and cultivation on greenhouse conditions until de flowering time. One of the plants obtained presented interesting characteristics, as good and rapid vegetative development and high quality of blooming. This hybrid obtained from an intergeneric crossing resulted in a plant with vegetative and flower type characteristics and color similar of Oncidium female parent, but with larger number of ramifications in inflorescence and number of flowers (60% and 219,4%, respectively) than Oncidium parent (♀), and with more similarity with Ionopsis (♂). This hybrid cultivar, called Ionocidium ‘Cerrado 101’ is one more option of OHGs in this competitive market and can be used either for pot and also for cut orchid flowers production, main because it longer inflorescence (83 cm).

scholarly journals Pre-incubation of porcine semen reduces the incidence of polyspermy on embryos derived from low quality oocytes

2016 ◽  
Vol 46 (6) ◽  
pp. 1113-1118 ◽  
Author(s):  
Cláudio Francisco Brogni ◽  
Lain Uriel Ohlweiler ◽  
Norton Klein ◽  
Joana Claudia Mezzalira ◽  
Jose Cristani ◽  
...  
Keyword(s):  
Cell Density ◽  
Embryo Development ◽  
High Quality ◽  
Chi Square ◽  
Fertilization Rates ◽  
Tukey Test ◽  
Chi Square Test ◽  
Low Efficiency

ABSTRACT: The main cause of low efficiency of in vitro produced porcine embryos is the high polyspermic penetration rates at fertilization, which is aggravated in low quality oocytes. Experiment 1 evaluated the embryo development in high and low quality oocytes. Experiment 2 evaluated the embryo development and quality of low quality oocytes fertilized with sperm pre-incubated during 0h (control), 0.5h, 1h and 1.5h. Experiment 3 investigated fertilization and monospermic rates of the same groups of Experiment 2. Experiment 4 evaluated embryo development, cell density, fertilization and monospermic rates of high quality oocytes using semen pre incubated during the best time observed in the previous experiments. Cleavage and blastocyst rates were analyzed by chi-square test, and remaining data by ANOVA and Tukey test (P≤0.05). The cleavage (74.8 vs 51.7%) and blastocyst (33.7 vs 9.8%) rates were greater in oocytes of high versus low quality, with no differences in cell density. Fertilization rates (65.6 to 79.5%) were not influenced by pre-incubation time. However, semen pre-incubation during 1.5h increased monospermic penetration (53.3%) and cleavage rates (92.5%) in low quality oocytes. Blastocyst rate was improved with 1.5h of semen pre incubation; however they were still lower than that observed with high quality control oocytes. Ultimately, pre-incubation did not influence fertilization, monospermic penetration, embryo development rates, nor cell density in oocytes of high quality. Low-quality porcine oocytes resulted in better rates of embryo development if in vitro fertilized with sperm pre-incubated for 1.5 hour.

scholarly journals Genealogy of Source Material for Its Use in Apple Breeding in Southern Russia

2017 ◽  
Vol 71 (3) ◽  
pp. 150-155
Author(s):  
Irina Dubravina ◽  
Igor Vasilenko ◽  
Irina Chepinoga ◽  
Sergey Gorlov
Keyword(s):  
Gene Pool ◽  
Gold Rush ◽  
Source Material ◽  
High Quality ◽  
Apple Trees ◽  
Breeding Station ◽  
Genealogical Analysis ◽  
Southern Russia ◽  
Apple Cultivars

Abstract Prebreeding research on Malus domestica Borkh. specimens was conducted to broaden the genetic diversity of cross breeding components for faster creation of cultivars of adaptive and technological orientation. Genealogical analysis has practical value in the study of the apple trees gene pool for accelerated development of cultivars with desired properties. Genealogical analysis of 55 apple cultivars from the gene pool of branch of the Department of Horticulture of Kuban State Agrarian University - Krymsk Experimental breeding station (Krymsk), complex donors were selected by traits of immunity to scab and high quality of fruits - ‘Gold Rush’, ‘Pristine’, ‘Enterprise’. Cultivar ‘Golden Delicious’ was selected as a “hidden” donor by the trait of medium (autumn) ripening time.

scholarly journals Recommendations for validation testing of home pregnancy tests (HPTs) in Europe

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Catharine Sturgeon ◽  
Stephen A. Butler ◽  
Fiona Gould ◽  
Sarah Johnson ◽  
Sam Rowlands ◽  
...  
Keyword(s):  
Best Practice ◽  
Clinical Sample ◽  
Laboratory Setting ◽  
High Quality ◽  
Validation Data ◽  
European Directive ◽  
Fit For Purpose ◽  
Validation Testing

Abstract Home pregnancy tests (HPTs) available in Europe include accuracy and other performance claims listed on their packaging. Due to the lack of guidance on the standardisation of such products, it is often difficult to replicate these claims when tested on a clinical sample, whether in a laboratory setting or by lay users. The In Vitro Diagnostic Regulation is a set of requirements that mandate comprehensive validation data on human pregnancy tests and other in vitro devices. It is due to replace the current European Directive (98/79/EC) and fully implemented in Europe by 2022. In June 2019, a panel of seven experts convened to discuss the validation studies required to provide the information needed to meet the new regulation for HPTs in Europe and proposed 15 recommendations for best practice. Defining best practice at all stages of validation of these important tests may ensure that tests marketed in Europe are fit for purpose, enabling lay users to be confident of the high quality of the HPT results they obtain. The panelists believe that the recommendations proposed here for the validation of HPTs may constructively contribute to improved standardisation of validation procedures in Europe.

scholarly journals Changes in fermentation pattern and quality of Italian ryegrass (Lolium multiflorum Lam.) silage by wilting and inoculant treatments

2021 ◽  
Vol 34 (1) ◽  
pp. 48-55
Author(s):  
Chang Liu ◽  
Guo Qiang Zhao ◽  
Sheng Nan Wei ◽  
Hak Jin Kim ◽  
Yan Fen Li ◽  
...  
Keyword(s):  
Dry Matter ◽  
Water Soluble ◽  
Italian Ryegrass ◽  
Neutral Detergent Fiber ◽  
High Quality ◽  
Dry Matter Digestibility ◽  
Fermentation Pattern ◽  
Additive Treatment

Objective: This study was conducted to investigate the effects of wilting and microbial inoculant treatment on the fermentation pattern and quality of Italian ryegrass silage.Methods: Italian ryegrass was harvested at heading stage and ensiled into vinyl bags (20 cm×30 cm) for 60d. Italian ryegrass was ensiled with 4 treatments (NWNA, no-wilting noadditive; NWA, no-wilting with additive; WNA, wilting no-additive; WA, wilting with additive) in 3 replications, wilting time was 5 hours and additives were treated with 10<sup>6</sup> cfu/g of <i>Lactobacillus plantarum</i>. The silages samples were collected at 1, 2, 3, 5, 10, 20, 30, 45, and 60 days after ensiling and analyzed for the ensiling quality and characteristics of fermentation patterns.Results: Wilting treatment resulted in lower crude protein and <i>in vitro</i> dry matter digestibility and there were no significant differences in acid detergent fiber (ADF), total digestible nutrient (TDN), water-soluble carbohydrate (WSC), ammonia content, and pH (p>0.05). However, wilting treatment resulted in higher ADF and neutral detergent fiber content of Italian ryegrass silage (p<0.05), and the WNA treatment showed the lowest TDN and <i>in vitro</i> dry matter digestibility. The pH of the silage was higher in the wilting group (WNA and WA) and lower in the additive treatment group. Meanwhile, the decrease in pH occurred sharply between the 3-5th day of storage. The ammonia nitrogen content was significantly lower in the additive treatment (p<0.05), and wilting had no effect. As fermentation progressed, the lactic and acetic acid contents were increased and showed the highest content at 30 days of storage.Conclusion: The wilting treatment did not significantly improve the silage fermentation, but the inoculant treatment improved the fermentation patterns and quality of the silage. So, inoculation before ensiling is recommended when preparing high quality of Italian ryegrass silage, and when wilting, it is recommended to combine inoculation for making high quality silage.

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