scholarly journals Synthesis of Ag-NPs from extracts of Persea americana and its antimicrobial effects in human pathogens

2020 ◽  
Vol 1 (1) ◽  
pp. 10-17
Author(s):  
Selvam K.A ◽  
◽  
Suriyakumar M ◽  
Devanathan J ◽  
Kolanjinathan K ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Streptococcus Mutans ◽  
Persea Americana ◽  
Human Beings ◽  
Human Pathogens ◽  
Zone Of Inhibition ◽  
Ag Nps ◽  
Leaf Extracts ◽  
Agar Disc ◽  
Vis Spectroscopy

Medicines of plant origin have been used to combat human diseases since time immemorial even before the history of their documentation. They have been used by human beings since time immemorial for curing health. The phytochemical screening of leaf extracts of Persea americanashows the presence of alkaloids, flavanoids, carbohydrates, saponins, protein, tannins and glycoside. Also the synthesis of silver nanoparticles was done using ethanolic Persea americana leaf extract. The synthesized silver nanoparticles were characterized by using Ultraviolet- visible (UV-Vis) spectroscopy, X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) analysis. The antimicrobial potential of synthesized nanoparticles was determined on the basis of mean diameter of zone of inhibition around the disc in millimeters. The antibacterial activity was evaluated qualitatively through agar disc diffusion towards Lactobacillus sp, Staphylococcus aureus, Streptococcus mutans, Klebsiella pneumoniae, Serratia marcescens, and Escherichia coli. The highest zone of inhibition value (15.0 mm) in Streptococcus mutans 15 ± 0.6. The antifungal activity against Aspergillus niger, Aspergillus flaves ,Candida albicans and Fusarium sp. From the results, excellent and eco-friendly green source for production of potential bio antimicrobial silver nanoparticles.

scholarly journals Green Synthesis, Characterization, and Antibacterial Properties of Silver Nanoparticles Obtained by Using Diverse Varieties of Cannabis sativa Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4041
Author(s):  
Adriana Cecilia Csakvari ◽  
Cristian Moisa ◽  
Dana G. Radu ◽  
Leonard M. Olariu ◽  
Andreea I. Lupitu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antioxidant Capacity ◽  
Cannabis Sativa ◽  
Antibacterial Properties ◽  
Green Building ◽  
Human Pathogens ◽  
Leaf Extracts ◽  
Vis Spectroscopy ◽  
Ft Ir

Cannabis sativa L. (hemp) is a plant used in the textile industry and green building material industry, as well as for the phytoremediation of soil, medical treatments, and supplementary food products. The synergistic effect of terpenes, flavonoids, and cannabinoids in hemp extracts may mediate the biogenic synthesis of metal nanoparticles. In this study, the chemical composition of aqueous leaf extracts of three varieties of Romanian hemp (two monoecious, and one dioecious) have been determined by Fourier-Transformed Infrared spectroscopy (FT-IR), high-performance liquid chromatography, and mass spectrometry (UHPLC-DAD-MS). Then, their capability to mediate the green synthesis of silver nanoparticles (AgNPs) and their pottential antibacterial applications were evaluated. The average antioxidant capacity of the extracts had 18.4 ± 3.9% inhibition determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 78.2 ± 4.1% determined by 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS™) assays. The total polyphenolic content of the extracts was 1642 ± 32 mg gallic acid equivalent (GAE) L−1. After this, these extracts were reacted with an aqueous solution of AgNO3 resulting in AgNPs, which were characterized by UV−VIS spectroscopy, FT-IR, scanning electron microscopy (SEM-EDX), and dynamic light scattering (DLS). The results demonstrated obtaining spherical, stable AgNPs with a diameter of less than 69 nm and an absorbance peak at 435 nm. The mixture of extracts and AgNPs showed a superior antioxidant capacity of 2.3 ± 0.4% inhibition determined by the DPPH• assay, 88.5 ± 0.9% inhibition as determined by the ABTS•+ assay, and a good antibacterial activity against several human pathogens: Escherichia coli, Klebsiella pneumoniae, Pseudomonas fluorescens, and Staphylococcus aureus.

scholarly journals Biogenic silver nanoparticles from Trichodesma indicum aqueous leaf extract against Mythimna separata and evaluation of its larvicidal efficacy

2017 ◽  
Vol 57 (2) ◽  
pp. 194-200 ◽  
Author(s):  
Abdul A. Buhroo ◽  
Gousul Nisa ◽  
Syed Asrafuzzaman ◽  
Ram Prasad ◽  
Razia Rasheed ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Average Particle ◽  
Particle Sizes ◽  
Mythimna Separata ◽  
Ag Nps ◽  
Leaf Extracts ◽  
Tem Analysis ◽  
Vis Spectroscopy ◽  
Aqueous Leaf Extract

AbstractThe present exploration is focused on the bio-fabrication of silver nanoparticles (Ag NPs) usingTrichodesma indicumaqueous leaf extract as a reducing agent. The synthesized Ag NPs were productively characterized by UV-vis spectroscopy, XRD, and TEM studies. The photosynthesis of Ag NPs was done at room temperature for 24 h and at 60°C. The green synthesis of spherical-shaped Ag NPs bio-fabricated fromT. indicumwith a face centred cubic structure showed average particle sizes of 20–50 nm, which is inconsistent with the particle size calculated by the XRD Scherer equation and TEM analysis. We further explored the larvicidal efficacy of biosynthesized Ag NPs with leaf extracts ofT. indicumagainstMythimna separata. The results showed that Ag NPs (20–50 nm) ofT. indicumpossess good larvicidal activity againstM. separatawith an LC50of 500 ppm. Thus, we can advocate that Ag NPs of 20–50 nm size extracted fromT. indicummay be considered in the pest management programme ofM. separatain future.

scholarly journals Green Synthesis of Silver Nanoparticles and Evaluation of Their Antibacterial Activity against Multidrug-Resistant Bacteria and Wound Healing Efficacy Using a Murine Model

Antibiotics ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 902
Author(s):  
Vajravathi Lakkim ◽  
Madhava C. Reddy ◽  
Roja Rani Pallavali ◽  
Kakarla Raghava Reddy ◽  
Ch Venkata Reddy ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Multidrug Resistant ◽  
Biomedical Science ◽  
Resistant Bacteria ◽  
Zone Of Inhibition ◽  
Ag Nps ◽  
Bacteriolytic Activity ◽  
Vis Spectroscopy

Green nanotechnology has significant applications in various biomedical science fields. In this study, green-synthesized silver nanoparticles, prepared by using Catharanthus roseus and Azadirachta indica extracts, were characterized using UV–Vis spectroscopy, dynamic light scattering, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. Silver nanoparticles (Ag NPs) synthesized from leaf extracts of C. roseus and A. indica effectively inhibited the growth of multidrug-resistant (MDR) bacteria isolated from patients with septic wound infections. The maximum bacteriolytic activity of the green-synthesized Ag NPs of C. roseus and A. indica against the MDR bacterium K. Pneumoniae was shown by a zone of inhibition of 19 and 16 mm, respectively. C. roseus Ag NPs exhibited more bacteriolytic activity than A. indica Ag NPs in terms of the zone of inhibition. Moreover, these particles were effective in healing wounds in BALB/c mice. Ag NPs of C. roseus and A. indica enhanced wound healing by 94% ± 1% and 87% ± 1%, respectively. Our data suggest that Ag NPs from C. roseus and A. indicia ameliorate excision wounds, and wound healing could be due to their effective antimicrobial activity against MDR bacteria. Hence, these Ag NPs could be potential therapeutic agents for the treatment of wounds.

scholarly journals Potential antibacterial activity of crude extracts and silver nanoparticles synthesized from Sargassum wightii

2014 ◽  
Vol 3 (10) ◽  
pp. 322-325 ◽  
Author(s):  
Vinoth Kumar Thirumalairaj ◽  
Mahitha Puthanpurayal Vijayan ◽  
Geetharamani Durairaj ◽  
Lakshmanasenthil Shanmugaasokan ◽  
Rincy Yesudas ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Nitrate Solution ◽  
Silver Nitrate Solution ◽  
Zone Of Inhibition ◽  
Ag Nps ◽  
Sargassum Wightii ◽  
Solvent Extract ◽  
Vis Spectroscopy

The present work investigates the antibacterial activity of silver nanoparticles (Ag-NPs) synthesized by biological method using Sargassum wightii. The fresh live seaweed was collected from the Mandapam coast of Tamilnadu, India. Solvent extract was prepared using acetone, petroleum ether and methanol. Aqueous extract of the seaweed was also used for the synthesis of silver Ag-NPs. Seaweed extract is used as a reducing agent of 2mM silver nitrate solution for the synthesis of Ag-NPs. Periodical monitoring of reaction mixture was done using UV-vis spectroscopy at 300-750 nm. The scanning electron microscopy (SEM) of the sample confirms the presence of Ag-NPs. The antibacterial activity of solvent extract was done by Minimal inhibitory concentration (MIC) assay. The methanol extract of the seaweed at a concentration of 250µg/ml exhibited potent antimicrobial activity against the test microorganism. The zone of inhibition ranging from 8-14 mm was observed with different extracts. The antibacterial activity of the synthesized Ag-NPs against the organism was also done by MIC test. The MIC of Ag-NPs was found to be 130µg/ml for all pathogenic microorganisms selected for the study. The zone of inhibition against Bacillus cereus, Bacillus anhtracis, Staphylococcus aureus and Vibrio alginoyticus were found to be 10, 8, 10 and 9 mm, respectively. The synthesized Ag-NPs exhibited significant antimicrobial activity against the selected microorganisms than the solvent extract of seaweed.DOI: http://dx.doi.org/10.3329/icpj.v3i10.20337 International Current Pharmaceutical Journal, September 2014, 3(10): 322-325

scholarly journals Green synthesis of silver nanoparticles: Characterization and its potential biomedical applications

2021 ◽  
Vol 10 (1) ◽  
pp. 412-420
Author(s):  
Mona S. Alwhibi ◽  
Dina A. Soliman ◽  
Manal A. Awad ◽  
Asma B. Alangery ◽  
Horiah Al Dehaish ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Aloe Vera ◽  
Inhibition Zone ◽  
Biologically Active ◽  
Noble Metal Nanoparticles ◽  
Biological Synthesis ◽  
Ag Nps ◽  
Mouse Melanoma ◽  
Therapeutic Benefits ◽  
Vis Spectroscopy

Abstract In recent times, research on the synthesis of noble metal nanoparticles (NPs) has developed rapidly and attracted considerable attention. The use of plant extracts is the preferred mode for the biological synthesis of NPs due to the presence of biologically active constituents. Aloe vera is a plant endowed with therapeutic benefits especially in skincare due to its unique curative properties. The present study focused on an environmental friendly and rapid method of phytosynthesis of silver nanoparticles (Ag-NPs) using A. vera gel extract as a reductant. The synthesized Ag-NPs were characterized by transmission electron microscopy (TEM), UV-Vis spectroscopy, Fourier transform infrared (FTIR), and dynamic light scattering (DLS). TEM micrographs showed spherical-shaped synthesized Ag-NPs with a diameter of 50–100 nm. The UV-Vis spectrum displayed a broad absorption peak of surface plasmon resonance (SPR) at 450 nm. The mean size and size distribution of the formed Ag-NPs were investigated using the DLS technique. Antibacterial studies revealed zones of inhibition by Ag-NPs of A. vera (9 and 7 mm) against Pseudomonas aeruginosa and Escherichia coli, respectively. Furthermore, the antifungal activity was screened, based on the diameter of the growth inhibition zone using the synthesized Ag-NPs for different fungal strains. Anticancer activity of the synthesized Ag-NPs against the mouse melanoma F10B16 cell line revealed 100% inhibition with Ag-NPs at a concentration of 100 µg mL−1. The phytosynthesized Ag-NPs demonstrated a marked antimicrobial activity and also exhibited a potent cytotoxic effect against mouse melanoma F10B16 cells. The key findings of this study indicate that synthesized Ag-NPs exhibit profound therapeutic activity and could be potentially ideal alternatives in medicinal applications.

scholarly journals Optimization of Green Synthesis of Silver Nanoparticles from Leaf Extracts ofPimenta dioica(Allspice)

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Akshay Rajeev Geetha ◽  
Elizabeth George ◽  
Akshay Srinivasan ◽  
Jameel Shaik
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Synthesis Method ◽  
Leaf Extracts ◽  
Synthesis Of Nanoparticles ◽  
Hot Air ◽  
Vis Spectroscopy ◽  
Pimenta Dioica ◽  
First Time

Production of silver nanoparticles from the leaf extracts ofPimenta dioicais reported for the first time in this paper. Three different sets of leaves were utilized for the synthesis of nanoparticles—fresh, hot-air oven dried, and sun-dried. These nanoparticles were characterized using UV-Vis spectroscopy and AFM. The results were diverse in that different sizes were seen for different leaf conditions. Nanoparticles synthesized using sun-dried leaves (produced using a particular ratio (1 : 0.5) of the leaf extract sample and silver nitrate (1 mM), resp.) possessed the smallest sizes. We believe that further optimization of the current green-synthesis method would help in the production of monodispersed silver nanoparticles having great potential in treating several diseases.

scholarly journals Nanocatalytic Activity of Silver Nanoparticles (Ag-Nps) Fabricated using Camellia sinensis (Linn) Tender Leaf Extract and their Characterization

2020 ◽  
Vol 2 (1) ◽  
pp. 24
Keyword(s):  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Pancreatic Enzyme ◽  
Alpha Amylase ◽  
Assay Method ◽  
Ag Nps ◽  
Visible Absorption ◽  
Biological Reduction ◽  
Vis Spectroscopy ◽  
Uv Visible

Silver nanoparticles (Ag-NPs) were prepared by the biological reduction method. Green tea extract was taken as a reducing and stabilizing agent and silver nitrate as the metal precursor for nanoparticle synthesis. The formation of the silver nanoparticles was monitored visually and using UV-Visible absorption spectroscopy. The synthesized silver nanoparticles were characterized by UV-visible spectroscopy, FTIR, Zeta sizer, Zeta potential, and antimicrobial studies. Silver nanoparticles were also subjected to investigate nanocatalytic activity with standard pancreatic alpha-amylase and bacterial amylase enzyme by the DNS assay method. UV-Vis spectroscopy revealed the formation of silver nanoparticles by exhibiting the typical surface plasmon absorption maxima at 430 nm. Four major functional groups of bio-molecules such as phenol, carboxylic acid, protein, and alkyl group were recorded in FTIR spectra. The size of the nanoparticles ranges between 5nm and 150nm. The average size and size distribution of silver nanoparticles is 59.66nm. The zeta potential of the silver nanoparticle is negatively charged and rendered as a sharp peak at -31.7mV. Antimicrobial activity of silver nanoparticles exhibited the highest inhibition against Gram-negative bacteria than Gram-positive bacteria and yeast pathogens. Starch hydrolysis of Ag-NPs was studied with pancreatic alpha-amylase (tailor made), crude and purified bacterial amylase enzyme. The formation of reducing sugar was increased about 40-fold for a purified enzyme, 11-fold for the pancreatic enzyme, and 6-fold for crude bacterial enzyme incorporated with Ag-NPs over control. The present studies recommended that Ag-NPs have a significant role in the degradation of starch into reducing sugars by acting as a nanocatalyst.

scholarly journals Microwave-Assisted Green Synthesis of Silver Nanoparticles Using Juglans regia Leaf Extract and Evaluation of Their Physico-Chemical and Antibacterial Properties

Antibiotics ◽  
2018 ◽  
Vol 7 (3) ◽  
pp. 68 ◽  
Author(s):  
Mahsa Eshghi ◽  
Hamideh Vaghari ◽  
Yahya Najian ◽  
Mohammad Najian ◽  
Hoda Jafarizadeh-Malmiri ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Juglans Regia ◽  
Antibacterial Properties ◽  
Infrared Analysis ◽  
Ag Nps ◽  
Stabilizing Agents ◽  
Agno3 Solution ◽  
Transmission Electron ◽  
Vis Spectroscopy

Silver nanoparticles (Ag NPs) were synthesized using Juglans regia (J. regia) leaf extract, as both reducing and stabilizing agents through microwave irradiation method. The effects of a 1% (w/v) amount of leaf extract (0.1–0.9 mL) and an amount of 1 mM AgNO3 solution (15–25 mL) on the broad emission peak (λmax) and concentration of the synthesized Ag NPs solution were investigated using response surface methodology (RSM). Fourier transform infrared analysis indicated the main functional groups existing in the J. regia leaf extract. Dynamic light scattering, UV-Vis spectroscopy and transmission electron microscopy were used to characterize the synthesized Ag NPs. Fabricated Ag NPs with the mean particle size and polydispersity index and maximum concentration and zeta potential of 168 nm, 0.419, 135.16 ppm and −15.6 mV, respectively, were obtained using 0.1 mL of J. regia leaf extract and 15 mL of AgNO3. The antibacterial activity of the fabricated Ag NPs was assessed against both Gram negative (Escherichia coli) and positive (Staphylococcus aureus) bacteria and was found to possess high bactericidal effects.

scholarly journals Green Synthesis of Silver Nanoparticles in the Presence of Polysaccharide: Optimization and Characterization

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
Margarita I. Skiba ◽  
Victoria I. Vorobyova ◽  
Alexander Pivovarov ◽  
Natalya P. Makarshenko
Keyword(s):  
Silver Nanoparticles ◽  
Sodium Alginate ◽  
Xrd Analysis ◽  
Face Centered Cubic ◽  
Colloidal Solutions ◽  
Ag Nps ◽  
Sem Images ◽  
Spherical Powder ◽  
Stabilizing Agent ◽  
Vis Spectroscopy

The process of obtaining aqueous solutions of silver nanoparticles with the use of a low-temperature nonequilibrium contact plasma and stabilizing agent—polysaccharide (sodium alginate)—has been examined. The synthesized Ag NPs were characterized by using UV-Vis spectroscopy, dynamic light scattering (DLS), scanning electron microscope (SEM), and XRD analysis. The effect of concentration of Ag+, sodium alginate, duration of processing by plasma discharge, and pH of liquid on the production of silver nanoparticles has been studied. The results demonstrated that synthesis provides the formation of silver nanoparticles for investigated concentrations of Ag+ (0.3-3.0 mmol/l) and 5.0 g/l Na-Alg (pH=7–10) within 1–5 minutes. From the SEM images, the silver nanoparticles are found to be almost spherical. Powder XRD results reveal that Ag nanoparticles have a face-centered cubic crystal structure. Zeta potential of plasma-chemically obtained colloidal solutions at various concentrations of Ag+ ions and stabilizing agent varies from −32.8 to −39.3 mV, indicating the moderate stability of synthesized nanoparticles.

scholarly journals Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain ofPseudomonas aeruginosaand Activity as Broad Spectrum Clinical Antibacterial Agents

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Melisa A. Quinteros ◽  
Ivana M. Aiassa Martínez ◽  
Pablo R. Dalmasso ◽  
Paulina L. Páez
Keyword(s):  
Silver Nanoparticles ◽  
Broad Spectrum ◽  
Nitrate Solution ◽  
Human Neutrophils ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Ag Nps ◽  
Tem Analysis ◽  
Vis Spectroscopy

Currently, the biosynthesis of silver-based nanomaterials attracts enormous attention owing to the documented antimicrobial properties of these ones. This study reports the extracellular biosynthesis of silver nanoparticles (Ag-NPs) using aPseudomonas aeruginosastrain from a reference culture collection. A greenish culture supernatant ofP. aeruginosaincubated at 37°C with a silver nitrate solution for 24 h changed to a yellowish brown color, indicating the formation of Ag-NPs, which was confirmed by UV-vis spectroscopy, transmission electron microscopy, and X-ray diffraction. TEM analysis showed spherical and pseudospherical nanoparticles with a distributed size mainly between 25 and 45 nm, and the XRD pattern revealed the crystalline nature of Ag-NPs. Also it provides an evaluation of the antimicrobial activity of the biosynthesized Ag-NPs against human pathogenic and opportunistic microorganisms, namely,Staphylococcus aureus,Staphylococcus epidermidis,Enterococcus faecalis,Proteus mirabilis,Acinetobacter baumannii,Escherichia coli,P. aeruginosa, andKlebsiella pneumonia. Ag-NPs were found to be bioactive at picomolar concentration levels showing bactericidal effects against both Gram-positive and Gram-negative bacterial strains. This work demonstrates the first helpful use of biosynthesized Ag-NPs as broad spectrum bactericidal agents for clinical strains of pathogenic multidrug-resistant bacteria such as methicillin-resistantS. aureus,A. baumannii, andE. coli. In addition, these Ag-NPs showed negligible cytotoxic effect in human neutrophils suggesting low toxicity to the host.

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