scholarly journals Fatty acid profile, antioxidant and antibacterial effect of the ethyl acatate extract of cleistopholis patens

2021 ◽  
Vol 3 (1) ◽  
pp. 21-31
Author(s):  
Daniels A.O ◽  
Taye Temikotan

Cleistopholis patens is a tropical plant that is used in the treatment of many bacterial and fungal infections. In this study, the antibacterial action against some human pathogens and the fatty acid profile of the plant were investigated. The plant was found to be active against Salmonella typhi Streptococcus pyogenes, Staphylococcus aureus while Shigella dysenteriae was resistant. The comparative antibiotic test revealed that only Staphylococcus aureus was sensitive to chloramphenicol and gentamycin. The purified extract showed lesser activity than the crude extract.  Phytochemical components include; glycosides, steroids, Phenol, tanins and saponins. Anticonstituents include tanin (2.32mg/g), phenol (2.50 mg/g), phytate (15.65 mg/g), oxalate (6.57 mg/g), saponin (9.71 mg/g) and flavonoids (6.49 mg/g). The GCMS profile of the ethyl acetate extract of the plant revealed 23 fatty acid including 9-Hexadecanoic acid, (25.11%), 6 octadecanoic acid (21.98%), n Hexadecanoic (4.62%), Cyclopentaneundecanoic acid, methyl ester (2.05%). Heptacosanoic acid, methyl ester (2.05%), Decanoic acid, methyl ester (1.74%), Oleic acid (4.38%), 16-Octadecenoic acid, methyl ester (3.55%) amongst others.

2003 ◽  
Vol 58 (7-8) ◽  
pp. 502-504 ◽  
Author(s):  
Ahmet C. Gören ◽  
Gökhan Bilsel ◽  
Mehmet Altun ◽  
Fatih Satıl

Abstract The chemical composition of fatty acid methyl esters (FAMEs) from seeds of S. thymbra and S. cuneifolia were analyzed by GC/MS. 7 FAMEs were identified from the seeds of S. thymbra mainly as 9-octadecenoic acid methyl ester (43.9%), hexadecanoic acid methyl ester (11.4%), 9,12,15-octadecatrienoic acid methyl ester (Z,Z,Z) (30.2%), and octadecanoic acid methyl ester (14.1%), while from the seed of S. cuneifolia 10 FAMEs were obtained with the main components, similar to S. thymbra. These were identified as 9-octadecenoic acid methyl ester (10.1%), hexadecanoic acid methyl ester (methyl palmitate, 34.6%), 9,12,15-octadecatrienoic acid methyl ester (Z,Z,Z) (6.3%) and octadecanoic acid methyl ester (1.8%).


KOVALEN ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 206-211
Author(s):  
Sumarni ◽  
Erwin Abdul Rahim ◽  
Ni Ketut Sumarni ◽  
Ruslan ◽  
Hardi Ys. ◽  
...  

Research on the manufacture of methyl esters from avocado seeds (Parsea americana Mill) with eugenol-based catalysts has been conducted. The aim is to determine the catalyst concentration used to produce methyl esters with the highest rendement and determine the composition of fatty acid methyl ester in avocado seeds. This study was used variations in concentrations of 0.25%, 1%, 1.75%, 2.25%, and 3%. The results of this study showed that the best concentration is 2.25% with the calculation of the results of 24.8% methyl esters in avocado seeds, namely lignoceric and octadecenoic acid methyl ester. Keywords: Avocado seeds, fatty acid methyl esters


Author(s):  
Suwaiba Muhammad Ladan ◽  
Yusuf Wada ◽  
Mustapha Mohammed ◽  
Sani Ibrahim ◽  
Kabir Musa Yusuf ◽  
...  

Senna alata (Linn) Roxb. plant is widely used to manage various infections in folkloric medicine. Methicillin-resistant Staphylococcus aureus (MRSA) infection continues to be a major global public health problem. This study aims to investigate the bioactive components of S. alata leaves active against MRSA. The leaves of S. alata were sequentially extracted and fractionated using standard methods and screened for activities against MRSA. The diethyl ether active thin layer chromatography (TLC) spot was subjected to infrared (IR) and gas chromatography-mass spectroscopic (GC-MS) studies. The aqueous extract and diethyl ether fraction of S. alata leaves elicited the highest activity against the MRSA. The GC-MS analysis of the fraction produced 15 eluates; only the sub-fraction 13 was effective. The TLC analysis of the sub-fraction 13 revealed three spots; only the second spot produced activity. The GC-MS result of the spot showed six peaks. The spectral results for peak 3 match the data from the IR study suggestive of 9-octadecenoic acid methyl ester. Senna alata leaves possess bioactive compounds closely related to 9-octadecenoic acid methyl ester with potent antibacterial activity against MRSA.


2021 ◽  
Vol 11 (1) ◽  
pp. 25
Author(s):  
Horasdia Saragih

<p><span>Fatty acid methyl esters (FAME, biodiesel) have been synthesized using a mixture of calcium hydroxide [Ca(OH)<sub>2</sub>] and calcite [CaCO<sub>3</sub>] catalyst. The fabrication was carried out by transesterifying triglycerides from palm oil using methanol at temperature of 60<sup>o</sup>C. The mixture of calcium hydroxide [Ca(OH)<sub>2</sub>] and calcite [CaCO<sub>3</sub>] catalyst was synthesized from CaO by exposed CaO to the air at room temperature. Various transesterification times have been involved in transesterification process. Based on the results obtained, it was found that the mixture of Ca(OH)<sub>2</sub> and CaCO<sub>3</sub> has catalytic characteristics, so that it can transesterify triglycerides and produce the FAME. There are 10 types of FAME produced from the palm oil triglycerides in this transesterification. Five of this types were saturated FAME and others were unsaturated FAME. The highest concentration of FAME is cis-9-octadecenoic acid methyl ester. The longer transesterification process, the more FAME is produced. Referring to the results of this study, it shows that the mixture of Ca(OH)<sub>2</sub> and CaCO<sub>3</sub> has the potential to be used as a catalyst for synthesizing biodiesel in the future.</span></p>


2021 ◽  
Vol 1 (3) ◽  
pp. 520-536
Author(s):  
Suwaiba Ladan Muhammad ◽  
Yusuf Wada ◽  
Mustapha Mohammed ◽  
Sani Ibrahim ◽  
Kabir Yusuf Musa ◽  
...  

Senna alata (Linn) Roxb. plant is widely used to manage various infections in folkloric medicine. Methicillin-resistant Staphylococcus aureus (MRSA) infection continues to be a major global public health problem. This study aims to investigate the bioactive components of S. alata leaves active against MRSA. The leaves of S. alata were sequentially extracted and fractionated using standard methods and screened for activities against MRSA. The diethyl ether active thin layer chromatography (TLC) spot was subjected to infrared (IR) and gas chromatography-mass spectroscopic (GC-MS) studies. The aqueous extract and diethyl ether fraction of S. alata leaves elicited the highest activity against the MRSA. The GC-MS analysis of the fraction produced 15 eluates; only the sub-fraction 13 was effective. The TLC analysis of the sub-fraction 13 revealed three spots; only the second spot produced activity. The GC-MS result of the spot showed six peaks. The spectral results for peak 3 match the data from the IR study, suggestive of 9-octadecenoic acid methyl ester. Senna alata leaves possess bioactive compounds closely related to 9-octadecenoic acid methyl ester with potent antibacterial activity against MRSA.


2013 ◽  
Author(s):  
Jr Morris ◽  
Shardo Robert W. ◽  
Higgins James ◽  
Cook Kim ◽  
Tanner Rhonda ◽  
...  

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