scholarly journals Evaluation of the Effects of 810 nm Diode Laser Alone and in Combination With Gluma© and Chromophore on Dentinal Tubule Occlusion: A Scanning Electron Microscopic Analysis

2020 ◽  
Vol 11 (3) ◽  
pp. 268-273
Author(s):  
Zahra Khoubrouypak ◽  
Masoumeh Hasani Tabatabaei ◽  
Nasim Chiniforush ◽  
Zohreh Moradi
Keyword(s):  
Laser Irradiation ◽  
Diode Laser ◽  
Smear Layer ◽  
Dentin Hypersensitivity ◽  
Pairwise Comparisons ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Layer Removal ◽  
Irradiation Energy ◽  
Scanning Electron

Introduction: Tooth hypersensitivity is among the most common patient complaints caused by the response of exposed dentin to external stimuli. No definite treatment has been suggested so far for dentin hypersensitivity (DH). This study aimed to compare the efficacy of the diode laser alone and in combination with Gluma and chromophore in occluding opened dentine tubules and the treatment of DH. Methods: This in vitro study was conducted on 30 extracted human third molars kept in 0.1% thymol solution. The teeth were mounted in transparent acrylic resin and the buccal enamel was cut to expose the outer third of dentin. The samples were then divided into 6 groups of negative control (no smear layer removal), positive control (smear layer removal with 6% citric acid application), 810 nm diode laser irradiation (energy density 55.55 J/cm2 , 1 W for 20 seconds in a continuous mode), chromophore (1 mg/mL indocyanine green, ICG) plus diode laser irradiation (1 W, 20 seconds), Gluma plus diode laser irradiation, and Gluma + chromophore + diode laser. Dentinal tubules were evaluated under a scanning electron microscope at x2000 magnification. The mean percentage of the obstruction of dentinal tubules was reported as mean and standard deviation. Considering the normal distribution of the data, two-way ANOVA was applied to compare the efficacy of treatments, and an independent-samples t test was used for pairwise comparisons at P<0.05 level of significance. Results: The highest mean percentage of the obstruction of dentinal tubules was noted in the diode laser/chromophore/Gluma group (65.68±12.31%) while the lowest value was noted in the diode laser/Gluma group (24.33±5.90%). Pairwise comparisons revealed significant differences between all groups (P<0.05) except for the difference between the laser/Gluma/chromophore and laser/ chromophore groups (P=0.20). Conclusion: It seems that chromophore increases the efficacy of the diode laser for the obstruction of dentinal tubules.

scholarly journals EFFICACY OF NOVEL ROOT CANAL IRRTIGANT-MTAD AGAINST ROOT BIOMODIFIERS ON PERIODONTALLY INVOVLED TEETH IN PERIODONTAL REGENRATION- AN IN VITRO SCANNING ELECTRON MICROSCOPY STUDY.

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Dasarapu Srujan ◽  
Gaurav Malhotra ◽  
Pradeep Shukla ◽  
Prerna Kataria ◽  
Mona Dagar
Keyword(s):  
Electron Microscope ◽  
Citric Acid ◽  
Scanning Electron Microscope ◽  
Ethylenediaminetetraacetic Acid ◽  
Smear Layer ◽  
Human Teeth ◽  
Dentinal Tubules ◽  
Layer Removal ◽  
Scanning Electron

Background: Smear layer removal and collagen fiber exposure may improve regeneration which can be accomplished by use of root biomodifiers. These enhance the degree of connective tissue attachment to denuded roots. The objective of this in vitro study was to evaluate and compare novel root canal irrigant and other root biomodifiers for smear layer removal on periodontally involved human teeth. Materials and Methods: Forty human teeth were collected and stored in saline. After scaling and root planing, two samples were obtained from each tooth. A total of 100 dentin blocks were randomly divided into four groups: Mixture of tetracycline, acid and detergent (MTAD), tetracycline hydrochloride (TTC HCl), citric acid (CA), and ethylenediaminetetraacetic acid (EDTA). The agents were applied for 3 min by active burnishing. Immediately following treatment, the specimens were rinsed, dehydrated, fixed and prepared for scanning electron microscope and were examined at × 3500 magnification. Sampaio’s index was evaluated by the previously trained blind examiner using photomicrographs.  Groups  were  compared  using  analysis  of  variance  followed  by  Tukey’s  post?hoc  test. Results: Mixture of tetracycline, acid, and detergent is most efficacious in removing the smear layer and showed statistically significant dentinal tubules opening, followed by EDTA, TTC HCl, and CA. Conclusion: Mixture of tetracycline, acid and detergent and conventional root biomodifiers used in the study alters the dentin surface by smear layer removal and exposure of dentinal tubules. Hence, MTAD as a root biomodifier may have a significant role in periodontal regeneration. Key words: Citric acid, ethylenediaminetetraacetic acid, MTAD, root biomodifier, scanning electron microscope study, tetracycline hydrochloride

Effects of Diode Laser Irradiation on Smear Layer Removal from Root Canal Walls and Apical Leakage after Obturation

2005 ◽  
Vol 23 (6) ◽  
pp. 575-581 ◽  
Author(s):  
Xiaogu Wang ◽  
Yichao Sun ◽  
Yuichi Kimura ◽  
Jun-Ichiro Kinoshita ◽  
Nelson Tatsunari Ishizaki ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Diode Laser ◽  
Root Canal ◽  
Smear Layer ◽  
Layer Removal ◽  
Apical Leakage

scholarly journals The Effectiveness of Three Different Plant Extracts Used as Irrigant in Removal of Smear Layer: A Scanning Electron Microscope Study

2015 ◽  
Vol 9 (1) ◽  
pp. 16-22 ◽  
Author(s):  
A Gupta ◽  
V Goyal ◽  
J Duhan ◽  
S Hans ◽  
P Sangwan
Keyword(s):  
Plant Extracts ◽  
Root Canal ◽  
Smear Layer ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Syzygium Aromaticum ◽  
Distilled Water ◽  
Scanning Electron Microscope Study ◽  
Layer Removal ◽  
Scanning Electron

ABSTRACT Aim In the present study, the role of three plant extracts as irrigant in root canal cleaning after instrumentation was evaluated. The effect of Syzygium aromaticum (S. Aromaticum), Ocimum sanctum (O. Sanctum) and Cinnamomum zeylanicum (C. zeylanicum) plant extracts was evaluated in smear layer removal. Methods The study was divided into different groups having 5 teeth each using various irrigating agents to evaluate smear layer removal. Group A: O. Sanctum extract; group A1: O. Sanctum extract with EDTA, group B: S. Aromaticum extract; group B1 S. Aromaticum extract with EDTA, group C: C. zeylanicum extract; group C1 C. zeylanicum extract with EDTA and two control group of 5 teeth each in group D: 3% NaOCl; group D1 3% NaOCl with EDTA (as positive control) and group E: Distilled water (as negative control); group E1 3% Distilled water with EDTA. Each tooth was split longitudinally and prepared for examination by scanning electron microscopy. Results The herbal extracts were effective in cleaning root canal walls when combine with EDTA with maximum activity of S. Aromaticum extract with EDTA group. Conclusion Under the condition of present study the three herbal plant extracts were ineffective in removal of smear layer when used alone.

scholarly journals Effects of Dentifrices Containing Nanohydroxyapatite on Dentinal Tubule Occlusion—A Scanning Electron Microscopy and EDX Study

2020 ◽  
Vol 10 (18) ◽  
pp. 6513
Author(s):  
Emilia Bologa ◽  
Simona Stoleriu ◽  
Gianina Iovan ◽  
Cristina Angela Ghiorghe ◽  
Irina Nica ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Study Groups ◽  
Control Group ◽  
Dentinal Tubule ◽  
Human Teeth ◽  
Dentinal Tubules ◽  
Mineral Deposition ◽  
Tubule Occlusion ◽  
Scanning Electron

This in vitro study evaluated the effects of dentifrices containing nano-hydroxyapatite (n-HAp) on dentinal tubule occlusion and on mineral deposition. Dentin specimens of ten human teeth were submersed for 30 s in 40% citric acid and then randomly divided into four groups (three study groups and one control group). In the study groups, the dentin samples were exposed to three different n-HAp toothpastes: Karex (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany), Biorepair Plus Sensitive (Coswell SpA, Bologna, Italy), and Dr. Wolff’s Biorepair (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany); in the control group no toothpaste was applied. All of the samples were evaluated using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis. In the control group all of the samples showed a frank and wide opening of the dentinal tubules, whereas in the study groups different degrees of tubule closure by mineral depositions were observed. Toothpastes containing n-HAp determined a significant occlusion of dentinal tubules and a significant increase of mineral deposition on the dentin surface. All three tested toothpastes showed similar results regarding the degree of dentinal tubule closure. Varying degrees of differences in calcium, phosphate, carbon, and oxygen ion concentrations among the three tested toothpastes were obtained.

Scanning Electron Microscopic Preliminary Study of the Efficacy of SmearClear and EDTA for Smear Layer Removal after Root Canal Instrumentation in Permanent Teeth

2008 ◽  
Vol 34 (12) ◽  
pp. 1541-1544 ◽  
Author(s):  
Lea Assed Bezerra da Silva ◽  
Ana Carolina Meng Sanguino ◽  
Cristiane Tomaz Rocha ◽  
Mario Roberto Leonardo ◽  
Raquel Assed Bezerra Silva
Keyword(s):  
Root Canal ◽  
Smear Layer ◽  
Permanent Teeth ◽  
Electron Microscopic ◽  
Layer Removal ◽  
Scanning Electron Microscopic ◽  
Preliminary Study ◽  
Root Canal Instrumentation ◽  
Scanning Electron
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