scholarly journals Effects of Dentifrices Containing Nanohydroxyapatite on Dentinal Tubule Occlusion—A Scanning Electron Microscopy and EDX Study

2020 ◽  
Vol 10 (18) ◽  
pp. 6513
Author(s):  
Emilia Bologa ◽  
Simona Stoleriu ◽  
Gianina Iovan ◽  
Cristina Angela Ghiorghe ◽  
Irina Nica ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Study Groups ◽  
Control Group ◽  
Dentinal Tubule ◽  
Human Teeth ◽  
Dentinal Tubules ◽  
Mineral Deposition ◽  
Tubule Occlusion ◽  
Scanning Electron

This in vitro study evaluated the effects of dentifrices containing nano-hydroxyapatite (n-HAp) on dentinal tubule occlusion and on mineral deposition. Dentin specimens of ten human teeth were submersed for 30 s in 40% citric acid and then randomly divided into four groups (three study groups and one control group). In the study groups, the dentin samples were exposed to three different n-HAp toothpastes: Karex (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany), Biorepair Plus Sensitive (Coswell SpA, Bologna, Italy), and Dr. Wolff’s Biorepair (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany); in the control group no toothpaste was applied. All of the samples were evaluated using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis. In the control group all of the samples showed a frank and wide opening of the dentinal tubules, whereas in the study groups different degrees of tubule closure by mineral depositions were observed. Toothpastes containing n-HAp determined a significant occlusion of dentinal tubules and a significant increase of mineral deposition on the dentin surface. All three tested toothpastes showed similar results regarding the degree of dentinal tubule closure. Varying degrees of differences in calcium, phosphate, carbon, and oxygen ion concentrations among the three tested toothpastes were obtained.

scholarly journals Perbedaan efektifitas penutupan tubulus dentin antara pasta gigi yang mengandung bioaktif glass (novamin) dan strontium chloride

2019 ◽  
Vol 8 (2) ◽  
pp. 96
Author(s):  
Profilia Shinta ◽  
Ketut Suardita ◽  
Moch. Mudjiono
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bioactive Glass ◽  
Strontium Chloride ◽  
Dentinal Tubule ◽  
Two Samples ◽  
Dentinal Hypersensitivity ◽  
Tubule Occlusion ◽  
Scanning Electron ◽  
At Home

Background : Dentinal hypersensitivity (DH) is a painful clinical condition and is characterized by pain arising from exposed dentin in response to various stimuli. Various treatment modalities are available to treat dentinal hypersensitivity which include at-home and in-office treatment. At home treatment generally consists of a variety of dentrifices containing different constituents like strontium chloride and bioactive glass (Novamin). These agents cause occlusion of dentinal tubules thereby reducing hypersensitivity.Purpose: This study  was  to evaluate the effects of different desensitizing dentifrices on dentinal tubule occlusion by scanning electron microscopy (SEM) of strontium chloride and bioactive glass (Novamin) pastes in the treatment of dentinehypersensitivity (DH) Methods: sixteen extractedspecimens from bovine incisors teeth with randomized into 2 groups (n=8). The crowns were removed from the root and the crown  were sectioned longitudinally into two parts (in a mesiodistaldirection). The cervical toothwere resulting in two samples per tooth.Dentinal tubules were exposedand thesamples provided a 4 mm x 4 mm area of exposed dentinal tubules.Group 1 (treated with strontium chloride), Group 2 (treated with  bioactive glass (novamin))After each treatment for seven days, tubule occlusion on dentin were analyzed by scanning electron microscopy  (SEM).The data were analyzed using “mann-whitneytest” (p<0.05).Result: Groups bioactive glass (novamin) showed tubule occlusion highest when compared with groups strontium chloride.Conclusion: bioactive glass (novamin) paste showed tubule occlusion highest with strontium chloride

The Evaluation of Dentinal Tubule Occlusion by Desensitizing Agents: A Real-time Measurement of Dentinal Fluid Flow Rate and Scanning Electron Microscopy

2013 ◽  
Vol 38 (4) ◽  
pp. 419-428 ◽  
Author(s):  
SY Kim ◽  
EJ Kim ◽  
DS Kim ◽  
IB Lee
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fluid Flow ◽  
Dentinal Tubule ◽  
Different Types ◽  
Dentinal Fluid Flow ◽  
Desensitizing Agents ◽  
Light Curing ◽  
Tubule Occlusion ◽  
Scanning Electron

SUMMARY The aims of this study were to examine changes in dentinal fluid flow (DFF) during the application of a desensitizing agent and to compare the permeability reduction levels among different types of desensitizing agents. A cervical cavity was prepared for the exposure of cervical dentin on an extracted human premolar connected to a subnanoliter fluid flow measuring device under 20 cm of water pressure. The cavity was acid-etched with 32% phosphoric acid to make dentin highly permeable. The different types of desensitizing agents that were applied on the cavity were Seal&Protect as the light-curing adhesive type, SuperSeal and BisBlock as oxalate types, Gluma Desensitizer as the protein-precipitation type, and Bi-Fluoride 12 as the fluoride type. DFF was measured from the time before the application of the desensitizing agent throughout the application procedure to five minutes after the application. The characteristics of dentinal tubule occlusion of each desensitizing agent were examined by scanning electron microscopy. The DFF rate after each desensitizing agent application was significantly reduced when compared to the initial DFF rate before application for all of the desensitizing agents (p&lt;0.05). Seal&Protect showed a greater reduction in the DFF rate when compared to Gluma Desensitizer and Bi-Fluoride 12 (p&lt;0.05). SuperSeal and BisBlock exhibited a greater reduction in DFF rate when compared to Bi-Fluoride 12 (p&lt;0.05). The dentin hypersensitivity treatment effects of the employed desensitizing agents in this study were confirmed through real-time measurements of DFF changes. The light-curing adhesive and oxalate types showed greater reduction in the DFF rate than did the protein-precipitation and fluoride types.

scholarly journals A scanning electron microscopy study of root surface smear layer removal after topical application of EDTA plus a detergent

2005 ◽  
Vol 13 (3) ◽  
pp. 247-252 ◽  
Author(s):  
José Eduardo Cezar Sampaio ◽  
Flávia Pavan Campos ◽  
Gibson Luiz Pilatti ◽  
Letícia Helena Theodoro ◽  
Fábio Renato Manzolli Leite
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Topical Application ◽  
Microscopy Study ◽  
Root Surface ◽  
Smear Layer ◽  
Control Group ◽  
Human Teeth ◽  
Layer Removal ◽  
Scanning Electron

The aim of the present study was to compare root surface smear layer removal following topical application of EDTA and EDTA-T (Texapon). Extracted human teeth had their cementum removed and were mechanically scaled. A total of 220 root specimens were obtained and were randomly assigned to the following groups: I-saline solution (control), II-EDTA; III-EDTA-T. Groups II and III specimens were assigned to different EDTA gel concentrations: 5%, 10%, 15%, 20% and 24%. Smear layer removal score was assessed for each specimen by scanning electron microscopy. The results demonstrated that EDTA and EDTA-T gel led to a higher root surface smear layer removal when compared to the control group. The 5% EDTA gel also showed a higher smear layer removal than the 15%, 20% and 24% EDTA gels (p<0.05). No difference could be found between the different concentrations of EDTA-T gels tested (p>0.05). EDTA gels had statistically significantly lower smear layer scores than the EDTA-T gels for the 5% and 10% concentrations. The results suggested that topical application of EDTA or EDTA-T gel led to significant smear layer removal of the mechanically treated root surfaces. The addition of a detergent to the EDTA gel formula did not improve smear layer removal of the root surface.

Penetration of Epiphany, Epiphany Self-Etch, and AH Plus into Dentinal Tubules: A Scanning Electron Microscopy Study

2011 ◽  
Vol 37 (9) ◽  
pp. 1316-1319 ◽  
Author(s):  
Noushin Shokouhinejad ◽  
Mohammad Sabeti ◽  
Hedayat Gorjestani ◽  
Mohammad Ali Saghiri ◽  
Mehrdad Lotfi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Dentinal Tubules ◽  
Scanning Electron Microscopy Study ◽  
Ah Plus ◽  
Self Etch ◽  
Scanning Electron

Scanning electron microscopy investigation of tooth/filling bonding

e-Polymers ◽  
2005 ◽  
Vol 5 (1) ◽  
Author(s):  
Joanna Siejka-Kulczyk ◽  
Małgorzata Lewandowska ◽  
Marta Raczyńska ◽  
Elżbieta Jodkowska ◽  
Krzysztof J. Kurzydłowski
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Teeth ◽  
Bonding Quality ◽  
Dental Fillings ◽  
Microscopy Investigation ◽  
Electron Microscopy Investigation ◽  
Scanning Electron Microscopy Investigation ◽  
Tissue Material ◽  
Scanning Electron

AbstractScanning electron microscopy (SEM) investigations have been conducted to determine the influence of selected material on tissue/material bonding. Extracted human teeth were used in this study. Several resin composites were tested as dental fillings. To reveal the structure of the tissue/filling interface, the teeth were cut parallel to the long axis. Bonding quality was determined by direct SEM observation and microanalysis with X-ray energy spectrometry (EDX). The investigated materials show good bonding with enamel.

scholarly journals Influence of Hydroxyapatite Nanospheres in Dentin Adhesive on the Dentin Bond Integrity and Degree of Conversion: A Scanning Electron Microscopy (SEM), Raman, Fourier Transform-Infrared (FTIR), and Microtensile Study

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2948
Author(s):  
Rana S Al-Hamdan ◽  
Basil Almutairi ◽  
Hiba F Kattan ◽  
Noura A. Alsuwailem ◽  
Imran Farooq ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Study Groups ◽  
Degree Of Conversion ◽  
Hybrid Layer ◽  
X Ray ◽  
And Control ◽  
Scanning Electron

An experimental adhesive incorporated with different nano-hydroxyapatite (n-HA) particle concentrations was synthesized and analyzed for dentin interaction, micro-tensile bond strength (μTBS), and degree of conversion (DC). n-HA powder (5 wt % and 10 wt %) were added in adhesive to yield three groups; gp-1: control experimental adhesive (CEA, 0 wt % HA), gp-2: 5 wt % n-HA (HAA-5%), and gp-3: 10 wt % n-HA (HAA-10%). The morphology of n-HA spheres was evaluated using Scanning Electron Microscopy (SEM). Their interaction in the adhesives was identified with SEM, Energy-Dispersive X-ray (EDX), and Micro-Raman spectroscopy. Teeth were sectioned, divided in study groups, and assessed for μTBS and failure mode. Employing Fourier Transform-Infrared (FTIR) spectroscopy, the DC of the adhesives was assessed. EDX mapping revealed the occurrence of oxygen, calcium, and phosphorus in the HAA-5% and HAA-10% groups. HAA-5% had the greatest μTBS values followed by HAA-10%. The presence of apatite was shown by FTIR spectra and Micro-Raman demonstrated phosphate and carbonate groups for n-HA spheres. The highest DC was observed for the CEA group followed by HAA-5%. n-HA spheres exhibited dentin interaction and formed a hybrid layer with resin tags. HAA-5% demonstrated superior μTBS compared with HAA-10% and control adhesive. The DC for HAA-5% was comparable to control adhesive.

Scanning Electron Microscopy of the Apical Structure of Human Teeth

2007 ◽  
Vol 31 (4) ◽  
pp. 321-325 ◽  
Author(s):  
Mário Roberto Leonardo ◽  
Marcos Antonio Rossi ◽  
Kleber Cortês Bonifácio ◽  
Léa Assed Bezerra da Silva ◽  
Sada Assed
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Teeth ◽  
Scanning Electron
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