scholarly journals Comparison of Antibacterial Effects of Photodynamic Therapy and an Irrigation Activation System on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

2020 ◽  
Vol 11 (3) ◽  
pp. 243-248
Author(s):  
Mohammad Asnaashari ◽  
Niloufar Kooshki ◽  
Mohammad Mahdi Salehi ◽  
Saranaz Azari-Marhabi ◽  
Hedieh Amin Moghadassi
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
New Technologies ◽  
Irrigation System ◽  
Bacterial Colony ◽  
Root Canals ◽  
Infected Root ◽  
Activation System

Introduction: Enterococcus faecalis is a resistant bacterium which is the most abundant species in infected root canals. Photodynamic therapy (PDT) is a method for killing the bacteria with active Oxygen radicals generated in a photosensitizer when exposed to centralized light. Furthermore, as a new method of canal disinfection, a variety of irrigation activation systems have been introduced, one of which is GentleFile (GF) with rotary movements and spiral effects for antibacterial action. This study aimed to compare the effectiveness of the two mentioned methods when used with and without Sodium Hypochlorite in eliminating E. faecalis from infected root canals. Methods: Fifty-eight uniradicular teeth were randomly divided into 4 experimental groups of 14. Two specimens were selected for later scanning electron microscopy in order to screen the procedure steps. In each experimental group, 10 samples were selected to be treated with GF or PDT; 3 of them were selected as positive controls and the other one sample was chosen as a negative control. Experimental groups were as follows: (1) Irrigation activation system, (2) Irrigation activation system + sodium hypochlorite, (3) PDT, and (4) PDT+ sodium hypochlorite. The specimens were then cultured for a bacterial colony count. Results: The decrease in the bacterial count after the treatment with the irrigation activation system was 99.8% (P=0.011) and when the system was used with sodium hypochlorite, it was 100% (P=0.001). The antibacterial effect of PDT was 90.08% (P=0.011) and it was 99.7% when PDT was combined with sodium hypochlorite (P=0.011). Conclusion: All four methods can be administered as complementary methods in root canal disinfection. According to the results of disinfection in the experimental groups of current study it is concluded that integration of new technologies such as activation irrigation system or PDT in Combination with NaOCl ameliorates disinfection of root canal and can provide several advantages in the endodontic outcome.

scholarly journals COMPARATIVE EVALUATION OF ROOT CANAL DISINFECTION USING 3% SODIUM HYPOCHLORITE, 940NM DIODE LASER AND PHOTODYNAMIC THERAPY IN SINGLE SITTING PULPECTOMY: AN IN-VIVO STUDY

2020 ◽  
pp. 1-3
Author(s):  
Thomas T Parakkal ◽  
Pulkit Jhingan ◽  
Shivani Mathur ◽  
Vinod Sachdev
Keyword(s):  
Photodynamic Therapy ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Bacterial Species ◽  
Root Canals ◽  
Root Canal Disinfection ◽  
Infected Root ◽  
Colony Forming Units

Introduction: Enterococcus faecalis is a common bacterial species in resistant and recurrent infections. It has an active proton pump which prevents its easy inhibition leading to endodontic treatment failure. LASER and photodynamic therapy have been employed lately in improving antimicrobial chemotherapy of localized infections. Aim: To compare disinfection efficacy of 940nm Diode LASER & Photodynamic therapy(PDT) to Conventional method using 3% Sodium hypochlorite in canals of endodontically treated primary teeth. Method: Single sitting Pulpectomy was performed on 60 mandibular first primary molars, divided into 3 groups, disinfected using mentioned modalities. Disinfection potential was compared collecting samples at 3 stages using paper points, cultured and incubated for 24hrs to check change in Colony Forming Units of the bacteria. Results: Data analysis with SPSS version 20 software using Kruskal-Wallis and Mann Whitney-U test. Conclusion: The three groups showed effective disinfection as revealed by the reduction in the colony forming units of the bacteria at baseline and after intervention. However, diode laser when used in conjunction with sodium hypochlorite as a disinfecting modality was found to be the most effective for the eradication of Enterococcus faecalis from infected root canals.

Antimicrobial Effectiveness of Photodynamic Therapy, 5% Sodium Hypochlorite and 2% Chlorhexidine Gluconate in Root Canal Treated Teeth: A Clinical Study

2021 ◽  
pp. 232020682110189
Author(s):  
Ramesh Bharti ◽  
Aseem Prakash Tikku ◽  
Anil Chandra ◽  
Prashant Gupta
Keyword(s):  
Photodynamic Therapy ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Chlorhexidine Gluconate ◽  
Total Bacterial Count ◽  
Root Canals ◽  
Anterior Teeth ◽  
Infected Root

Objective: To evaluate the effectiveness of photodynamic therapy (PDT), sodium hypochlorite (NaOCl) and chlorhexidine gluconate (CHO) in the endodontic re-treatment in the removing of microorganisms from previously filled infected root canals. Materials and Methods: The study samples consist of 54 root canal treated anterior teeth with apical periodontitis were randomly divided into three groups according to the disinfection protocol. Microbiological samples from the root canals were collected after removing the gutta-percha (S1) and following the endodontic re-treatment (S2) with either 5% NaOCl, 2% CHO or PDT procedure for 1 minute. Microbiological samples were cultivated on selective plates to assess the presence or absence of microbial growth and determine the average reduction of viable microorganisms. Results: Significant reduction in the total bacterial count in all the groups at post treatment ( P < .001). PDT provided statistically higher (99.74%) elimination of Enterococcus faecalis in comparison to NaOCl (95%) and CHO (88%). Conclusions: The use of PDT in endodontic re-treatment led to the significant reduction of the remaining bacterial species. So, PDT can be used for routine endodontic disinfection in failed root canal cases.

scholarly journals Antibacterial Effects of Erbium Chromium Laser along with/without Silver Nanoparticles in Root Canals Infected by Enterococcus faecalis

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Seyedeh Sareh Hendi ◽  
Negin Amiri ◽  
Banafsheh Poormoradi ◽  
Mohammad Yousef Alikhani ◽  
Saeid Afshar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Silver Nanoparticle ◽  
Colony Count ◽  
Antibacterial Effects ◽  
Bacterial Colony ◽  
Root Canals ◽  
Significant Difference ◽  
Root Canal System

This study investigates the antibacterial effects of erbium chromium laser at 2780 nm, silver nanoparticles, and erbium chromium along with silver nanoparticles on Enterococcus faecalis in comparison with sodium hypochlorite. In the present study, 90 extracted human single-rooted teeth were selected and standardized to a length of 15 mm. The canals were prepared by V-taper Gold rotary files and then incubated with E. faecalis for 21 days. The samples were divided into four experimental groups including hypochlorite sodium, silver nanoparticle, erbium chromium laser, and erbium chromium laser along with silver nanoparticle groups. Results showed that there was a significant reduction in colony count for all groups after interventions. Moreover, there was a significant reduction in the colony count for sodium hypochlorite group in comparison with another groups, and this group showed the highest reduction of colony count. There was a significant difference between silver nanoparticles and erbium chromium laser groups in colony count. According to the results, the silver nanoparticles offered strong antibacterial effects on E. faecalis and therefore can decrease bacterial colonies, while the use of the laser, despite the reduction of the bacterial colony, could not be sufficiently used for disinfection of root canal system.

scholarly journals Antibacterial Potential of 2.5% Sodium Hypochlorite in Distinct Irrigation Protocols on Enterococcus faecalis Biofilm

2015 ◽  
Vol 16 (5) ◽  
pp. 340-346
Author(s):  
Cyntia Rodrigues de Araújo Estrela ◽  
Ana Helena Gonçalves de Alencar ◽  
Carlos Estrela ◽  
Denise Ramos Silveira Alves ◽  
Rodrigo Sanches Cunha ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Negative Pressure ◽  
Uv Spectrophotometry ◽  
Sem Images ◽  
Root Canals ◽  
Irrigation Methods ◽  
Infected Root ◽  
The Mean ◽  
Antibacterial Potential

ABSTRACT Objective The aim of this study was to evaluate the effect of irrigation methods on antibacterial potential of 2.5% NaOCl on Enterococcus faecalis biofilm. Materials and methods Enterococcus faecalis biofilms were prepared during 60 days on 48 human root canals and randomized into control and experimental groups using positive and negative pressure irrigation. Bacterial growth was analyzed using turbidity of culture medium followed by UV spectrophotometry, and scanning electron microscopy (SEM) analyses were performed. Mean and standard deviations were used for evaluate the mean optical densities associated to the number of bacteria present culture, and Scheirer-Ray-Hare (an extension of the Kruskal-Wallis test) and Tamhane test to analyze the SEM images in the groups and thirds. Significance was set at 5%. Results Enterococcus faecalis was still present after root canal cleaning regardless of irrigation methods or bacterial identification methods. Conclusion Positive and negative pressure irrigation protocols using 2.5% NaOCl show a similar capacity to reduce E. faecalis in infected root canals. How to cite this article Alves DRS, Cunha RS, da Silveira Bueno CE, de Alencar AHG, de Araújo Estrela CR, dos Santos TO, Estrela C. Antibacterial Potential of 2.5% Sodium Hypochlorite in Distinct Irrigation Protocols on Enterococcus faecalis Biofilm. J Contemp Dent Pract 2015;16(5):340-346.

scholarly journals Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis

2016 ◽  
Vol 17 (8) ◽  
pp. 650-653 ◽  
Author(s):  
Jain Mathew ◽  
Reshmi George ◽  
Robin Theruvil ◽  
Tobin C Padavil ◽  
Lincy Tomy ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Annona Muricata ◽  
Root Canals ◽  
Root Canal Irrigants ◽  
Simarouba Glauca

ABSTRACT Aim To determine the antimicrobial effect of water extracts of leaves of Annona muricata and Simarouba glauca on Enterococcus faecalis using agar diffusion method. Materials and methods Dried leaves of A. muricata and S. glauca were powdered and extracted in a soxhlet apparatus. Enterococcus faecalis was grown overnight in Trypticase soy agar plates. About 10 μL of each extract was placed on agar plates and incubated overnight. The zone of inhibition was measured after 24 hours. About 1% sodium hypochlorite and distilled water were used as positive and negative controls. Results The leaf extract of A. muricata showed similar effectiveness as that of sodium hypochlorite, whereas the leaf extract of S. glauca showed only a slight reduction in growth of E. faecalis. Conclusion Leaf extract of A. muricata can be developed as an alternative to sodium hypochlorite for root canal irrigants. Clinical significance Success of endodontic treatment depends on complete disinfection of the root canals. Root canal irrigants have a major role in complete disinfection of the root canals. Chemical root canal irrigants are more or less toxic to the oral environment. In this study, naturally derived leaf extracts of A. muricata and S. glauca are compared with sodium hypochlorite for its effectiveness against E. faecalis – the most common pathogen found in the root canals. How to cite this article Mathew J, George R, Theruvil R, Padavil TC, Tomy L, Kurian A. Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis. J Contemp Dent Pract 2016;17(8):650-653.

scholarly journals Evaluation of 4% Sodium Hypochlorite in eliminating Enterococcus faecalis from the Root Canal when Used with Three Irrigation Methods: An in vitro Study

2017 ◽  
Vol 18 (3) ◽  
pp. 214-217
Author(s):  
Vinisha Pandey ◽  
Harsh Priyank ◽  
Abhishek Bagul ◽  
Kishore Kumar Majety ◽  
Parul Verma ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Saline Solution ◽  
Brain Heart Infusion ◽  
Root Canals ◽  
Irrigation Methods ◽  
Group I ◽  
Significant Difference

ABSTRACT Introduction Endodontic treatment removes all pathogens, such as Enterococcus faecalis from pulp and root canals. The aim of this study is to assess the usefulness of sodium hypochlorite (NaOCl) in removing E. faecalis from the root canal used with three different irrigation methods. Materials and methods This study was conducted on freshly extracted maxillary incisors. After biomechanical preparation, root canals were injected with E. faecalis. Three groups were made which contained 30 teeth in each group; 2 mL of NaOCl solution was used for irrigation followed by agitation with K-files in group I; 2 mL of NaOCl solution was used for irrigation and ultrasonic agitation was done in group II. In group III, an alternate irrigation with NaOCl and 3% hydrogen peroxide was done. The fourth group (control) was irrigated with sterile saline solution. E. faecalis bacteria were sampled to the root canals with paper points and were transferred to tubes that contained 5 mL of brain heart infusion broth. Tubes were incubated and the presence of broth turbidity was suggestive of bacteria remaining in the root canal. Results All three groups showed no statistically significant difference. However, difference existed between experimental groups and control groups. Conclusion The author concluded that all three methods of application of NaOCl were effective in disinfecting the root canal than the saline solution. Clinical significance No single irrigant has 100% efficiency. Thus by this study, a best irrigating solution with maximum properties can be established. How to cite this article Priyank H, Pandey V, Bagul A, Majety KK, Verma P, Choudhury BK. Evaluation of 4% Sodium Hypochlorite in eliminating Enterococcus faecalis from the Root Canal when Used with Three Irrigation Methods: An in vitro Study. J Contemp Dent Pract 2017;18(3):214-217.

scholarly journals Comparison of Antibacterial Effects of Photodynamic Therapy, Modified Triple Antibiotic Paste and Calcium Hydroxide on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

2019 ◽  
Vol 10 (5) ◽  
pp. S23-S29 ◽  
Author(s):  
Mohammad Asnaashari ◽  
Mohammad Jafar Eghbal ◽  
Amirali Sahba Yaghmayi ◽  
Mehdi Shokri ◽  
Saranaz Azari-Marhabi
Keyword(s):  
Photodynamic Therapy ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Control Group ◽  
Root Canals ◽  
Anterior Teeth ◽  
Triple Antibiotic Paste ◽  
Working Length

Introduction: A pivotal issue to achieve success in the treatment of the root canal is root canal disinfection. One of the most important bacteria that infect the root canal is Enterococcus faecalis. This study seeks to examine the effectiveness of 3 methods for disinfecting the root canal: photodynamic therapy, modified triple antibiotic paste (MTAP), and calcium hydroxide. Methods: Sixty-two single-rooted extracted anterior teeth were collected. After cleaning and disinfecting the teeth, their crowns were cut at the CEJ point. The root canals were shaped to the working length up to file F3 ProTaper (F1, F2, F3). EDTA 17% and sodium hypochlorite 2.5% were used for 5 minutes to wash and remove the smear layer, and then the apical foramen was sealed using composite. After that, the teeth were sterilized in an autoclave at 121°C for 15 minutes. Then 10 samples were taken randomly as the negative control. The remaining samples were immersed and cultivated in a suspension containing E. faecalis for 21 days. Then the samples were divided into 5 groups: 2 positive control groups, 1 group treated with the antibiotic paste with the concentration of 1 mg/mL, 1 group treated with calcium hydroxide, and 1 group treated with photodynamic therapy. Then, to collect the biofilm, the ProTaper file F4 was used. After that, the microbial suspension was provided and counting the colonies was carried out to compare the groups. Results: The findings indicated that the amount of CFU/mg of MTAP samples, including clindamycin, metronidazole, ciprofloxacin in the concentration of 1 mg/mL and photodynamic therapy and calcium hydroxide was lower than that in the control group. Antibiotic paste cleansed the root canal up to 99.9%. Photodynamic therapy reduced the amount of CFU/mg to 98.8%, and calcium hydroxide reduced the amount of CFU/mg to 94.13%. Conclusion: Using photodynamic therapy causes a reduction in the biofilm and inhibits the growth of the E. faecalis bacterium. In addition, in this study, MTAP with a concentration of 1 mg/mL was used, which expunged the bacteria completely. Meanwhile, calcium hydroxide had the weakest effect of all on the E. faecalis bacterium.

Antimicrobial Activity of Calcium Hydroxide Synthesized from Indonesian Limestone against Enterococcus faecalis

2021 ◽  
Vol 1044 ◽  
pp. 171-177
Author(s):  
Myrna Nurlatifah Zakaria ◽  
Ida Rodiyah Siti Halimah ◽  
Atia Nurul Sidiqa ◽  
Ira Artilia ◽  
Arief Cahyanto
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antimicrobial Property ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Negative Control ◽  
Root Canals ◽  
Infected Root

Recurrent endodontic infections of treated teeth are mainly linked to microbial persistency in the root canal system. Enterococcus faecalis is a bacterium that is often identified in these recurrent infections. Calcium hydroxide (Ca(OH)2) is a widely used root canal medicament and is now being developed to be produced from natural materials. Ca(OH)2, which is synthesized from natural limestone from Palimanan, Indonesia, has a structure similar to Ca(OH)2, which is often used as a root canal medicament. The purpose of this study was to determine the antimicrobial property of Ca(OH)2 paste synthesized from natural limestone compared to conventional Ca(OH)2 paste. This study is an in vitro experimental study consists of 3 groups (n=24, namely, conventional Ca(OH)2 paste, limestone synthesized Ca(OH)2 paste, and saline as the negative control, for 2 intervals of time and evaluated by Total Plate Counting (TPC) method. Samples were mandibular first premolar root canal prepared by root canal preparation and contaminated with E. faecalis (ATCC 29212). The infected root canals of each group were treated by the medicaments, and samples from the root canals were taken on day 7 (n=4) and 14 (n=4). Results showed that both Ca(OH)2 groups had antimicrobial activities against E. faecalis. The limestone Ca(OH)2 paste group had the least amount of bacterial colonies on the 7th day compared to other groups. In contrast, on the 14th day, the conventional Ca(OH)2 paste had the least bacterial colonies. It can be concluded that the Ca(OH)2 paste synthesized from natural limestone has an antimicrobial effect on E. faecalis, the antimicrobial effect was higher on the 7th day but decreases on the 14th day, whereas the conventional Ca(OH)2 had a higher antimicrobial effect on day 14 compared to day 7. Further studies are expected to improve the long-term antimicrobial effectiveness and sustainability of the natural limestone synthesized Ca(OH)2 paste.

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