scholarly journals Molecular Epidemiology. Molecular Typing of Pathogenic Bacteria Using Pulsed-field Gel Electrophoresis.

2000 ◽  
Vol 55 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Satoru ICHIYAMA
Keyword(s):  
Molecular Epidemiology ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pathogenic Bacteria ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan

1999 ◽  
Vol 122 (2) ◽  
pp. 337-341 ◽  
Author(s):  
M. AKIBA ◽  
T. MASUDA ◽  
T. SAMESHIMA ◽  
K. KATSUDA ◽  
M. NAKAZAWA
Keyword(s):  
Escherichia Coli ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Outbreak Strain ◽  
Pulsed Field ◽  
E Coli ◽  
Biological Methods ◽  
Pfge Profiles

A total of 77 Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157[ratio ]H7 infection in Japan.

Donor-to-Recipient Transmission of Bacteria as an Unusual Cause of Mediastinitis in a Heart Transplant Recipient

1999 ◽  
Vol 20 (02) ◽  
pp. 132-133 ◽  
Author(s):  
Jeffrey S. Burket ◽  
Carol E. Chenoweth ◽  
Thomas L. Meyer ◽  
Neil L. Barg
Keyword(s):  
Transplant Recipient ◽  
Heart Transplant ◽  
Gel Electrophoresis ◽  
Pathogenic Bacteria ◽  
Klebsiella Oxytoca ◽  
Heart Transplant Recipient ◽  
Pulsed Field Gel Electrophoresis ◽  
Banding Patterns ◽  
Pulsed Field

AbstractWe present a 54-year-old male heart transplant recipient who developed mediastinitis caused byKlebsiella oxytocaandVeillonellaspecies. Culture of the donor's bronchus also grewK oxytocaand aVeillonellaspecies. Pulsed-field gel electrophoresis revealed that theK oxytocaisolates had identical banding patterns. This case illustrates transmission of pathogenic bacteria via a contaminated organ.

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