Faculty Opinions recommendation of Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation.

2009 ◽  
Author(s):  
Arnon Nagler
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Malignant Transformation ◽  
Human Mesenchymal Stem Cells

scholarly journals Long-term Cultures of Bone Marrow–Derived Human Mesenchymal Stem Cells Frequently Undergo Spontaneous Malignant Transformation

2009 ◽  
Vol 69 (13) ◽  
pp. 5331-5339 ◽  
Author(s):  
Gro Vatne Røsland ◽  
Agnete Svendsen ◽  
Anja Torsvik ◽  
Ewa Sobala ◽  
Emmet McCormack ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Malignant Transformation ◽  
Human Mesenchymal Stem Cells

scholarly journals Cytotoxic Effect of Hypoxic Environment in Mesenchymal Stem Cell

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1592
Author(s):  
Sevil Özer ◽  
H. Seda Vatansever ◽  
Feyzan Özdal-Kurt
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Mesenchymal Stem Cells ◽  
Hypoxic Condition ◽  
Immunoperoxidase Technique ◽  
Cellular Functions ◽  
Marrow Mesenchymal Stem Cells

Bone marrow mesenchymal stem cells (BM-MSCs) are used to repair hypoxic or ischemic tissue. After hypoxic the level of ATP is decreases, cellular functions do not continue and apoptosis or necrosis occur. Apoptosis is a progress of programmed cell death that occurs in normal or pathological conditions. In this study, we were investigated the hypoxic effect on apoptosis in mesenchymal stem cell. Bone marrow-derived stem cells were cultured in hypoxic (1% or 3%) or normoxic conditions 24, 96 well plates for 36 h. Cell viability was shown by MTT assay on 36 h. After fixation of cells with 4% paraformaldehyde, distributions of caspase-3, Bcl-2 and Bax with indirect immunoperoxidase technique, apoptotic cells with TUNEL assay were investigated. All staining results were evaluated using H-score analyses method with ANOVA, statistically. As a result, hypoxic condition was toxic for human mesenchymal stem cells and the number of death cell was higher in that than normoxic condition.

Long-term cultured mesenchymal stem cells frequently develop genomic mutations but do not undergo malignant transformation

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S75-S76
Author(s):  
Y. Wang ◽  
Z. Han ◽  
Z. Zhang ◽  
Y. Chi ◽  
Z. Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Malignant Transformation

Evaluation of Canine Bone Marrow-derived Mesenchymal Stem Cells After Long-term Cryopreservation

2013 ◽  
Vol 30 (12) ◽  
pp. 1032-1037 ◽  
Author(s):  
Xiaofeng Zhu ◽  
Fang Yuan ◽  
Houxuan Li ◽  
Yuqian Zheng ◽  
Yin Xiao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells

Malignant transformation of rat bone marrow-derived mesenchymal stem cells treated with 4-nitroquinoline 1-oxide

2010 ◽  
Vol 188 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Shujuan Zhang ◽  
Zhijuan Han ◽  
Qingfei Kong ◽  
Jinghua Wang ◽  
Bo Sun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Malignant Transformation ◽  
Rat Bone
Sign in / Sign up

Export Citation Format

Share Document