Faculty Opinions recommendation of tRNAs in Trypanosoma brucei: genomic organization, expression, and mitochondrial import.

2002 ◽  
Author(s):  
C Graham Clark
Keyword(s):  
Trypanosoma Brucei ◽  
Genomic Organization ◽  
Mitochondrial Import

scholarly journals tRNAs in Trypanosoma brucei: Genomic Organization, Expression, and Mitochondrial Import

2002 ◽  
Vol 22 (11) ◽  
pp. 3707-3717 ◽  
Author(s):  
Timothy H. P. Tan ◽  
Roland Pach ◽  
Anne Crausaz ◽  
Al Ivens ◽  
André Schneider
Keyword(s):  
Trypanosoma Brucei ◽  
Genomic Organization ◽  
Northern Analysis ◽  
Trna Genes ◽  
Mitochondrial Localization ◽  
Genomic Context ◽  
Absolute Abundance ◽  
Mitochondrial Import ◽  
Transgenic Cell

ABSTRACT The mitochondrial genome of Trypanosoma brucei does not encode tRNAs. Consequently, all mitochondrial tRNAs are imported from the cytosol and originate from nucleus-encoded genes. Analysis of all currently available T. brucei sequences revealed that its genome carries 50 tRNA genes representing 40 different isoacceptors. The identified set is expected to be nearly complete since all but four codons are accounted for. The number of tRNA genes in T. brucei is very low for a eukaryote and lower than those of many prokaryotes. Using quantitative Northern analysis we have determined the absolute abundance in the cell and the mitochondrion of a group of 15 tRNAs specific for 12 amino acids. Except for the initiator type tRNAMet, which is cytosol specific, the cytosolic and the mitochondrial sets of tRNAs were qualitatively identical. However, the extent of mitochondrial localization was variable for the different tRNAs, ranging from 1 to 7.5% per cell. Finally, by using transgenic cell lines in combination with quantitative Northern analysis it was shown that import of tRNALeu(CAA) is independent of its 5′-genomic context, suggesting that the in vivo import substrate corresponds to the mature, fully processed tRNA.

Genomic organization and expression of elongation factor-1α genes in Trypanosoma brucei

1996 ◽  
Vol 79 (1) ◽  
pp. 119-123 ◽  
Author(s):  
Evelyn L. Ridgley ◽  
Zhao-hui Xiong ◽  
Kiran J. Kaur ◽  
Larry Ruben
Keyword(s):  
Trypanosoma Brucei ◽  
Genomic Organization ◽  
Elongation Factor ◽  
Elongation Factor 1Α

Genomic organization of Trypanosoma brucei variant antigen gene families in sequential parasitemias

1983 ◽  
Vol 9 (3) ◽  
pp. 255-269 ◽  
Author(s):  
Marilyn Parsons ◽  
Richard G. Nelson ◽  
George Newport ◽  
Michael Milhausen ◽  
Kenneth Stuart ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Genomic Organization ◽  
Gene Families ◽  
Antigen Gene ◽  
Variant Antigen

scholarly journals Novel IM‐associated protein Tim54 plays a role in the mitochondrial import of internal signal‐containing proteins in Trypanosoma brucei

2020 ◽  
Author(s):  
Ujjal K. Singha ◽  
Anuj Tripathi ◽  
Joseph T. Smith ◽  
Linda Quinones ◽  
Aparajita Saha ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Mitochondrial Import ◽  
Internal Signal

Genomic organization of variant surface glycoprotein genes in Trypanosoma brucei procyclic culture forms

1983 ◽  
Vol 23 (1-4) ◽  
pp. 27-33 ◽  
Author(s):  
Marilyn Parsons ◽  
Richard G. Nelson ◽  
Kenneth Stuart ◽  
Nina Agabian
Keyword(s):  
Trypanosoma Brucei ◽  
Genomic Organization ◽  
Surface Glycoprotein ◽  
Variant Surface Glycoprotein

Screening of some Asteraceae to discover new active compounds against Trypanosoma brucei by metabolite profiling and PLS analysis

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
MS Nogueira ◽  
FB da Costa ◽  
MA Magenta ◽  
M Kaiser ◽  
R Brun ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Metabolite Profiling ◽  
Active Compounds ◽  
Pls Analysis

Localization of the Br Polymorphism on a 144 bp Exon of the GPIa Gene and Its Application in Platelet DNA Typing

1994 ◽  
Vol 71 (05) ◽  
pp. 651-654 ◽  
Author(s):  
Rainer Kalb ◽  
Sentot Santoso ◽  
Katja Unkelbach ◽  
Volker Kiefel ◽  
Christian Mueller-Eckhardt
Keyword(s):  
Genomic Organization ◽  
Fragment Length Polymorphism ◽  
Human Platelet ◽  
Dna Typing ◽  
Rflp Analysis ◽  
Serological Typing ◽  
Allele Specific ◽  
Polymerase Chain ◽  
Alloimmune Thrombocytopenia ◽  
Rflp Typing

SummaryAlloimmunization against the human platelet alloantigen system Br (HPA-5) is the second most common cause of neonatal alloimmune thrombocytopenia (NAIT) in Caucasian populations. We have recently shown that a single base polymorphism at position 1648 on platelet mRNA coding for GPIa results in an aminoacid substitution at position 505 on the mature GPIa which is associated with the two serological defined Br phenotypes.Since DNA-typing of platelet alloantigens offers possibilities for useful clinical applications, we designed genomic DNA-based restriction fragment length polymorphism (RFLP) typing for Br alloantigens. To establish this technique we analyzed the genomic organization of GPIa adjacent to the polymorphic base. Using the polymerase chain reaction (PCR) of blood cell DNA we have identified two introns (approximately 1.7 and 1.9 kb) flanking a 144 bp coding sequence of the GPIa gene encompassing the polymorphic base 1648. Based on the in- tron sequence, a PCR primer was constructed to amplify a 274 bp fragment which was used for allele-specific RFLP to determine the Br genotypes. The results of RFLP analysis using Mnll endonuclease obtained from 15 donors (2 Br37*, 2 Br^ and 11 Brb/b) correlate perfectly with serological typing by monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay.

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