scholarly journals Novel IM‐associated protein Tim54 plays a role in the mitochondrial import of internal signal‐containing proteins in Trypanosoma brucei

2020 ◽  
Author(s):  
Ujjal K. Singha ◽  
Anuj Tripathi ◽  
Joseph T. Smith ◽  
Linda Quinones ◽  
Aparajita Saha ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Mitochondrial Import ◽  
Internal Signal

scholarly journals tRNAs in Trypanosoma brucei: Genomic Organization, Expression, and Mitochondrial Import

2002 ◽  
Vol 22 (11) ◽  
pp. 3707-3717 ◽  
Author(s):  
Timothy H. P. Tan ◽  
Roland Pach ◽  
Anne Crausaz ◽  
Al Ivens ◽  
André Schneider
Keyword(s):  
Trypanosoma Brucei ◽  
Genomic Organization ◽  
Northern Analysis ◽  
Trna Genes ◽  
Mitochondrial Localization ◽  
Genomic Context ◽  
Absolute Abundance ◽  
Mitochondrial Import ◽  
Transgenic Cell

ABSTRACT The mitochondrial genome of Trypanosoma brucei does not encode tRNAs. Consequently, all mitochondrial tRNAs are imported from the cytosol and originate from nucleus-encoded genes. Analysis of all currently available T. brucei sequences revealed that its genome carries 50 tRNA genes representing 40 different isoacceptors. The identified set is expected to be nearly complete since all but four codons are accounted for. The number of tRNA genes in T. brucei is very low for a eukaryote and lower than those of many prokaryotes. Using quantitative Northern analysis we have determined the absolute abundance in the cell and the mitochondrion of a group of 15 tRNAs specific for 12 amino acids. Except for the initiator type tRNAMet, which is cytosol specific, the cytosolic and the mitochondrial sets of tRNAs were qualitatively identical. However, the extent of mitochondrial localization was variable for the different tRNAs, ranging from 1 to 7.5% per cell. Finally, by using transgenic cell lines in combination with quantitative Northern analysis it was shown that import of tRNALeu(CAA) is independent of its 5′-genomic context, suggesting that the in vivo import substrate corresponds to the mature, fully processed tRNA.

Screening of some Asteraceae to discover new active compounds against Trypanosoma brucei by metabolite profiling and PLS analysis

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
MS Nogueira ◽  
FB da Costa ◽  
MA Magenta ◽  
M Kaiser ◽  
R Brun ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Metabolite Profiling ◽  
Active Compounds ◽  
Pls Analysis
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