Motility Parameters and Intracellular ATP Content of Rabbit Spermatozoa Stored for 3 Days at 15°C

2013 ◽  
Vol 61 (1) ◽  
pp. 87-91 ◽  
Author(s):  
Piotr Gogol
Keyword(s):  
Atp Content ◽  
Intracellular Atp ◽  
Rabbit Spermatozoa ◽  
Motility Parameters

Motility, mitochondrial membrane potential and ATP content of rabbit spermatozoa stored in extender supplemented with GnRH analogue [des-Gly10, D-Ala6]-LH-RH ethylamide

2014 ◽  
Vol 17 (4) ◽  
pp. 571-575 ◽  
Author(s):  
P. Gogol ◽  
M. Trzcińska ◽  
M. Bryła
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Sperm Motility ◽  
Mitochondrial Membrane ◽  
Gnrh Analogue ◽  
Atp Content ◽  
Straight Line ◽  
Lh Rh ◽  
Rabbit Spermatozoa ◽  
Motility Parameters

Abstract The present study was aimed to determine the effect of GnRH analogue [des-Gly10, D-Ala6]-LH-RH ethylamide on the quality of rabbit spermatozoa stored at 17°C for 3 days. Semen from 5 bucks (13 ejaculates) was used in the experiment. Ejaculates were divided and diluted at a 1:10 ratio with rabbit semen extender Galap (IMV, France) (Control) or with Galap extender supplemented with GnRH analogue [des-Gly10, D-Ala6]-LH-RH ethylamide (50 μg/ml) and stored for 3 days. Sperm motility parameters, mitochondrial membrane potential (MMP) and ATP content were assessed on each day of the experiment. Motility analysis was performed using a computer-assisted sperm analysis (CASA) system. The following sperm motility parameters were recorded: total motile spermatozoa, progressively motile spermatozoa, curvilinear velocity, straight-line velocity, average path velocity, linearity, straightness and amplitude of lateral head displacement. MMP was evaluated using JC-1 fluorescent dye. ATP content was assessed using a bioluminescence method. The addition of GnRH analogue [des-Gly10, D-Ala6]-LH-RH ethylamide to Galap extender did not affect any of the quality parameters studied. However, in both groups (Control and GnRH), significant changes in motility parameters (except straight-line velocity) and proportion of spermatozoa showing high MMP and ATP content were observed throughout 3 days of storage.

Decreased insulin binding and degradation associated with depressed intracellular ATP content

Diabetes ◽  
1980 ◽  
Vol 29 (3) ◽  
pp. 221-226 ◽  
Author(s):  
B. Draznin ◽  
C. C. Solomons ◽  
C. A. Emler ◽  
D. S. Schalch ◽  
K. E. Sussman
Keyword(s):  
Insulin Binding ◽  
Atp Content ◽  
Intracellular Atp

Selenium promotes proliferation of chondrogenic cell ATDC5 by increment of intracellular ATP content under serum deprivation

2012 ◽  
Vol 30 (8) ◽  
pp. 657-663 ◽  
Author(s):  
Jidong Yan ◽  
Juan Tian ◽  
Yuewen Zheng ◽  
Yan Han ◽  
Shemin Lu
Keyword(s):  
Serum Deprivation ◽  
Atp Content ◽  
Intracellular Atp

Glycolytic component of rat spermatid energy and acid-base metabolism

1990 ◽  
Vol 259 (4) ◽  
pp. C660-C667 ◽  
Author(s):  
J. G. Reyes ◽  
M. V. Velarde ◽  
R. Ugarte ◽  
D. J. Benos
Keyword(s):  
Lactate Production ◽  
Co2 Production ◽  
Simultaneous Occurrence ◽  
Atp Content ◽  
Extracellular Medium ◽  
Glucose Addition ◽  
Intracellular Atp ◽  
Acid Diffusion ◽  
Glucose Analogues ◽  
The Impact

The impact of glycolysis on rat spermatid energy metabolism is made apparent by the simultaneous occurrence of the following three events upon glucose addition to the extracellular medium of a rat spermatid cell suspension: decrease in ATP content, exit of acid equivalents, and increased lactate production and efflux. In this work, we have studied the interrelations between these three phenomena. By measuring ATP content, net acid transport, lactate exit, oxygen consumption, intracellular pH, CO2 production, and glycolytic intermediates in the presence of glucose and glucose analogues, we conclude that 1) lactate production, decrease in ATP content, and acid equivalent exit are dependent on the metabolism of glucose up to different stages in glycolysis. 2) The decrease in ATP content is not directly related to the exit of acid equivalents from rat spermatids. 3) Glucose metabolism is a net ATP-consuming process at high intracellular ATP content but is a net ATP-producing process at low intracellular ATP concentration in rat spermatids. 4) Acid equivalent production arises from the metabolism of glucose beyond glyceraldehyde 3-phosphate dehydrogenase. 5) Lactic acid diffusion and/or lactate transport and CO2 production and exit could account for the glucose-dependent acid equivalent efflux in rat spermatids.

scholarly journals Changes of intracellular ATP content and purine compounds in ischemic warm rat lung.

1996 ◽  
Vol 10 (1) ◽  
pp. 8-13
Author(s):  
Akinori Akashi ◽  
Kazuya Nakahara ◽  
Yoshitaka Fujii ◽  
Akihide Matsumura ◽  
Hikaru Matsuda
Keyword(s):  
Rat Lung ◽  
Atp Content ◽  
Intracellular Atp

Decreased Insulin Binding and Degradation Associated with Depressed Intracellular ATP Content

Diabetes ◽  
1980 ◽  
Vol 29 (3) ◽  
pp. 221-226 ◽  
Author(s):  
B. Draznin ◽  
C. C. Solomons ◽  
C. A. Emler ◽  
D. S. Schalch ◽  
K. E. Sussman
Keyword(s):  
Insulin Binding ◽  
Atp Content ◽  
Intracellular Atp

scholarly journals Complex I and ATP Content Deficiency in Lymphocytes from Friedreich's Ataxia

2009 ◽  
Vol 36 (1) ◽  
pp. 26-31 ◽  
Author(s):  
Mohammad Mehdi Heidari ◽  
Massoud Houshmand ◽  
Saman Hosseinkhani ◽  
Shahriar Nafissi ◽  
Mehri Khatami
Keyword(s):  
Respiratory Chain ◽  
Complex I ◽  
Age Of Onset ◽  
Friedreich’S Ataxia ◽  
Friedreich's Ataxia ◽  
Iron Accumulation ◽  
Atp Content ◽  
Respiratory Chain Complexes ◽  
Intracellular Atp ◽  
Complex I Activity

Background:Friedreich's ataxia (FRDA) is an inherited recessive disorder characterized by progressive neurological disability and heart abnormalities. A deficiency in the protein frataxin causes this disease. Frataxin deficiency leads to progressive iron accumulation in mitochondria, excessive free radical production and dysfunction of respiratory chain complexes. The expansion (GAA) repeat in the first intron causes decreased frataxin expression by interfering with transcription.Methods:Activity of mitochondrial respiratory chain complex I (measured as NADH ferricyanide reductase) and intracellular ATP measurement was performed on lymphocyte of FRDA patients (n=12) and control subjects (n=25).Results:Our findings showed that complex I activity and intracellular ATP were significantly reduced (P=0.001) in patients compared with controls and we found strong correlation between complex I activity and intracellular ATP content in FRDA patients (r = 0.93; P<0.002). 8.6 and 9.0 kb deletion in mtDNA was detected in 9 patients out of 12 (75%) by multiplex polymerase chain reaction (PCR) and Southern blot analysis.Conclusions:This study suggested that a biochemical defect in complex I activity and ATP production, which may be due to iron accumulation in mitochondria, can be involved in age of onset of FRDA.

The intracellular ATP content of fresh and cultured human islets isolated from different donors

1997 ◽  
Vol 29 (4) ◽  
pp. 1979 ◽  
Author(s):  
D. Brandhorst ◽  
H. Brandhorst ◽  
B.J. Hering ◽  
K. Federlin ◽  
R.G. Bretzel
Keyword(s):  
Human Islets ◽  
Atp Content ◽  
Intracellular Atp

scholarly journals Decreased Intracellular ATP Content and Intact Mitochondrial Energy Generating Capacity in Human Cystinotic Fibroblasts

2006 ◽  
Vol 59 (2) ◽  
pp. 287-292 ◽  
Author(s):  
Elena N Levtchenko ◽  
Martijn J G Wilmer ◽  
Antoon J M Janssen ◽  
Jan B Koenderink ◽  
Henk-Jan Visch ◽  
...  
Keyword(s):  
Atp Content ◽  
Intracellular Atp ◽  
Generating Capacity
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