Accumulation of nitrite and sulfite in yeast cells and synergistic depletion of the intracellular ATP content

1985 ◽  
Vol 180 (2) ◽  
pp. 117-120 ◽  
Author(s):  
Helga Hinze ◽  
Helmut Holzer
Keyword(s):  
Yeast Cells ◽  
Atp Content ◽  
Intracellular Atp

Decreased insulin binding and degradation associated with depressed intracellular ATP content

Diabetes ◽  
1980 ◽  
Vol 29 (3) ◽  
pp. 221-226 ◽  
Author(s):  
B. Draznin ◽  
C. C. Solomons ◽  
C. A. Emler ◽  
D. S. Schalch ◽  
K. E. Sussman
Keyword(s):  
Insulin Binding ◽  
Atp Content ◽  
Intracellular Atp

Selenium promotes proliferation of chondrogenic cell ATDC5 by increment of intracellular ATP content under serum deprivation

2012 ◽  
Vol 30 (8) ◽  
pp. 657-663 ◽  
Author(s):  
Jidong Yan ◽  
Juan Tian ◽  
Yuewen Zheng ◽  
Yan Han ◽  
Shemin Lu
Keyword(s):  
Serum Deprivation ◽  
Atp Content ◽  
Intracellular Atp

Glycolytic component of rat spermatid energy and acid-base metabolism

1990 ◽  
Vol 259 (4) ◽  
pp. C660-C667 ◽  
Author(s):  
J. G. Reyes ◽  
M. V. Velarde ◽  
R. Ugarte ◽  
D. J. Benos
Keyword(s):  
Lactate Production ◽  
Co2 Production ◽  
Simultaneous Occurrence ◽  
Atp Content ◽  
Extracellular Medium ◽  
Glucose Addition ◽  
Intracellular Atp ◽  
Acid Diffusion ◽  
Glucose Analogues ◽  
The Impact

The impact of glycolysis on rat spermatid energy metabolism is made apparent by the simultaneous occurrence of the following three events upon glucose addition to the extracellular medium of a rat spermatid cell suspension: decrease in ATP content, exit of acid equivalents, and increased lactate production and efflux. In this work, we have studied the interrelations between these three phenomena. By measuring ATP content, net acid transport, lactate exit, oxygen consumption, intracellular pH, CO2 production, and glycolytic intermediates in the presence of glucose and glucose analogues, we conclude that 1) lactate production, decrease in ATP content, and acid equivalent exit are dependent on the metabolism of glucose up to different stages in glycolysis. 2) The decrease in ATP content is not directly related to the exit of acid equivalents from rat spermatids. 3) Glucose metabolism is a net ATP-consuming process at high intracellular ATP content but is a net ATP-producing process at low intracellular ATP concentration in rat spermatids. 4) Acid equivalent production arises from the metabolism of glucose beyond glyceraldehyde 3-phosphate dehydrogenase. 5) Lactic acid diffusion and/or lactate transport and CO2 production and exit could account for the glucose-dependent acid equivalent efflux in rat spermatids.

Cell de-energization prevents plasmid transformation of yeast Saccharomyces cerevisiae: evidence for the requirement of ATP

2010 ◽  
Vol 5 (1) ◽  
pp. 78-82 ◽  
Author(s):  
Larisa Chaustova ◽  
Valė Miliukienė ◽  
Aurelijus Zimkus
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transformation Efficiency ◽  
Energy Requirement ◽  
Yeast Cells ◽  
Sodium Arsenate ◽  
Yeast Saccharomyces Cerevisiae ◽  
Plasmid Transformation ◽  
Substrate Level Phosphorylation ◽  
Intracellular Atp ◽  
Substrate Level

AbstractThe dependence of the yeast Saccharomyces cerevisiae transformation on energy requirement was studied. The inhibitory effect of sodium arsenate, used for the depletion of the intracellular ATP pool, was determined. Incubation of the yeast cells in 5 mM sodium arsenate diminished ATP accumulation by 50% and the transformation efficiency decreased by 65%. To discriminate between ATP produced by substrate level phosphorylation and oxidative phosphorylation, the inhibitory analysis of a mutant with defective mitochondria was performed. Sodium fluoride (10–50 mM), as inhibitor of glycolysis, elicited a concentration-dependent decrease in intracellular ATP levels in both parental and mutant cells. The equal transformation efficiency of the mitochondrial mutant and parental strain, in addition to experiments with oligomycin, demonstrated the independence of plasmid transformation on mitochondrial ATP synthesis. This is consistent with our hypothesis that yeast transformation efficiency is associated with ATP produced by substrate level phosphorylation.

scholarly journals Changes of intracellular ATP content and purine compounds in ischemic warm rat lung.

1996 ◽  
Vol 10 (1) ◽  
pp. 8-13
Author(s):  
Akinori Akashi ◽  
Kazuya Nakahara ◽  
Yoshitaka Fujii ◽  
Akihide Matsumura ◽  
Hikaru Matsuda
Keyword(s):  
Rat Lung ◽  
Atp Content ◽  
Intracellular Atp

Decreased Insulin Binding and Degradation Associated with Depressed Intracellular ATP Content

Diabetes ◽  
1980 ◽  
Vol 29 (3) ◽  
pp. 221-226 ◽  
Author(s):  
B. Draznin ◽  
C. C. Solomons ◽  
C. A. Emler ◽  
D. S. Schalch ◽  
K. E. Sussman
Keyword(s):  
Insulin Binding ◽  
Atp Content ◽  
Intracellular Atp

scholarly journals Complex I and ATP Content Deficiency in Lymphocytes from Friedreich's Ataxia

2009 ◽  
Vol 36 (1) ◽  
pp. 26-31 ◽  
Author(s):  
Mohammad Mehdi Heidari ◽  
Massoud Houshmand ◽  
Saman Hosseinkhani ◽  
Shahriar Nafissi ◽  
Mehri Khatami
Keyword(s):  
Respiratory Chain ◽  
Complex I ◽  
Age Of Onset ◽  
Friedreich’S Ataxia ◽  
Friedreich's Ataxia ◽  
Iron Accumulation ◽  
Atp Content ◽  
Respiratory Chain Complexes ◽  
Intracellular Atp ◽  
Complex I Activity

Background:Friedreich's ataxia (FRDA) is an inherited recessive disorder characterized by progressive neurological disability and heart abnormalities. A deficiency in the protein frataxin causes this disease. Frataxin deficiency leads to progressive iron accumulation in mitochondria, excessive free radical production and dysfunction of respiratory chain complexes. The expansion (GAA) repeat in the first intron causes decreased frataxin expression by interfering with transcription.Methods:Activity of mitochondrial respiratory chain complex I (measured as NADH ferricyanide reductase) and intracellular ATP measurement was performed on lymphocyte of FRDA patients (n=12) and control subjects (n=25).Results:Our findings showed that complex I activity and intracellular ATP were significantly reduced (P=0.001) in patients compared with controls and we found strong correlation between complex I activity and intracellular ATP content in FRDA patients (r = 0.93; P<0.002). 8.6 and 9.0 kb deletion in mtDNA was detected in 9 patients out of 12 (75%) by multiplex polymerase chain reaction (PCR) and Southern blot analysis.Conclusions:This study suggested that a biochemical defect in complex I activity and ATP production, which may be due to iron accumulation in mitochondria, can be involved in age of onset of FRDA.

The intracellular ATP content of fresh and cultured human islets isolated from different donors

1997 ◽  
Vol 29 (4) ◽  
pp. 1979 ◽  
Author(s):  
D. Brandhorst ◽  
H. Brandhorst ◽  
B.J. Hering ◽  
K. Federlin ◽  
R.G. Bretzel
Keyword(s):  
Human Islets ◽  
Atp Content ◽  
Intracellular Atp

scholarly journals Decreased Intracellular ATP Content and Intact Mitochondrial Energy Generating Capacity in Human Cystinotic Fibroblasts

2006 ◽  
Vol 59 (2) ◽  
pp. 287-292 ◽  
Author(s):  
Elena N Levtchenko ◽  
Martijn J G Wilmer ◽  
Antoon J M Janssen ◽  
Jan B Koenderink ◽  
Henk-Jan Visch ◽  
...  
Keyword(s):  
Atp Content ◽  
Intracellular Atp ◽  
Generating Capacity
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