scholarly journals Structural analysis of allosteric regulation of the regulatory GAF domains of cone photoreceptor phosphodiesterase-6 (PDE6) by cGMP and the inhibitory γ-subunit

2019 ◽  
Author(s):  
Richa Gupta ◽  
Yong Liu ◽  
Huanchen Wang ◽  
Christopher T. Nordyke ◽  
Ryan Z. Puterbaugh ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Allosteric Regulation ◽  
Cone Photoreceptor ◽  
Γ Subunit ◽  
Photoreceptor Phosphodiesterase

scholarly journals Direct Allosteric Regulation between the GAF Domain and Catalytic Domain of Photoreceptor Phosphodiesterase PDE6

2008 ◽  
Vol 283 (44) ◽  
pp. 29699-29705 ◽  
Author(s):  
Xiu-Jun Zhang ◽  
Karyn B. Cahill ◽  
Arye Elfenbein ◽  
Vadim Y. Arshavsky ◽  
Rick H. Cote
Keyword(s):  
Catalytic Domain ◽  
Allosteric Regulation ◽  
Photoreceptor Phosphodiesterase

scholarly journals Molecular Determinants of cGMP Binding to Chicken Cone Photoreceptor Phosphodiesterase

2004 ◽  
Vol 279 (46) ◽  
pp. 48143-48151 ◽  
Author(s):  
Daming Huang ◽  
Thomas R. Hinds ◽  
Sergio E. Martinez ◽  
Catalin Doneanu ◽  
Joseph A. Beavo
Keyword(s):  
Cone Photoreceptor ◽  
Molecular Determinants ◽  
Photoreceptor Phosphodiesterase

scholarly journals Structural Analysis of the Bacterial HPr Kinase/Phosphorylase V267F Mutant Gives Insights into the Allosteric Regulation Mechanism of This Bifunctional Enzyme

2007 ◽  
Vol 282 (48) ◽  
pp. 34952-34957 ◽  
Author(s):  
Vincent Chaptal ◽  
Fanny Vincent ◽  
Virginie Gueguen-Chaignon ◽  
Vicente Monedero ◽  
Sandrine Poncet ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Allosteric Regulation ◽  
Regulation Mechanism ◽  
Bifunctional Enzyme

scholarly journals Mutations in the gene PDE6C encoding the catalytic subunit of the cone photoreceptor phosphodiesterase in patients with achromatopsia

2018 ◽  
Vol 39 (10) ◽  
pp. 1366-1371 ◽  
Author(s):  
Nicole Weisschuh ◽  
Katarina Stingl ◽  
Isabelle Audo ◽  
Saskia Biskup ◽  
Béatrice Bocquet ◽  
...  
Keyword(s):  
Catalytic Subunit ◽  
Cone Photoreceptor ◽  
Photoreceptor Phosphodiesterase

scholarly journals Regulation of photoreceptor phosphodiesterase catalysis by its non-catalytic cGMP-binding sites

1999 ◽  
Vol 340 (3) ◽  
pp. 863-869 ◽  
Author(s):  
Marc R. D'AMOURS ◽  
Rick H. COTE
Keyword(s):  
Binding Sites ◽  
Outer Segment ◽  
Catalytic Domain ◽  
Hydrolytic Activity ◽  
Rod Photoreceptors ◽  
High Affinity ◽  
Γ Subunit ◽  
C Terminus ◽  
Catalytic Sites ◽  
Photoreceptor Phosphodiesterase

The photoreceptor 3ʹ:5ʹ-cyclic nucleotide phosphodiesterase (PDE) is the central enzyme of visual excitation in rod photoreceptors. The hydrolytic activity of PDE is precisely regulated by its inhibitory γ subunit (Pγ), which binds directly to the catalytic site. We examined the inhibition of frog rod outer segment PDE by endogenous Pγ, as well as by synthetic peptides corresponding to its central and C-terminal domains, to determine whether the non-catalytic cGMP-binding sites on the catalytic αβ dimer of PDE allosterically regulate PDE activity. We found that the apparent binding affinity of Pγ for PDE was 28 pM when cGMP occupied the non-catalytic sites, whereas Pγ had an apparent affinity only 1/16 of this when the sites were empty. The elevated basal activity of PDE with empty non-catalytic sites can be decreased by the addition of nanomolar levels of cGMP, demonstrating that the high-affinity non-catalytic sites on the PDE catalytic dimer mediate this effect. No evidence for a direct allosteric effect of the non-catalytic sites on catalysis could be detected for the activated enzyme lacking bound Pγ. The intrinsic affinity of a synthetic C-terminal (residues 63-87) Pγ peptide to bind and to inhibit the hydrolytic activity of activated PDE was enhanced 300-fold in the presence of cGMP compared with cAMP. We conclude that the binding of cGMP to the non-catalytic sites of PDE induces an allosteric change in the structure of the catalytic domain that greatly enhances the interaction of the C-terminus of Pγ with the catalytic domain.

scholarly journals Structural Analysis of the Regulatory Dithiol-containing Domain of the Chloroplast ATP Synthase γ Subunit

2006 ◽  
Vol 281 (41) ◽  
pp. 31041-31049 ◽  
Author(s):  
Hardeep S. Samra ◽  
Fei Gao ◽  
Feng He ◽  
Etter Hoang ◽  
Zugen Chen ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Atp Synthase ◽  
Chloroplast Atp Synthase ◽  
Γ Subunit
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