scholarly journals Functional Mapping of Interacting Regions of the Photoreceptor Phosphodiesterase (PDE6) γ-Subunit with PDE6 Catalytic Dimer, Transducin, and Regulator of G-protein Signaling9–1 (RGS9–1)

2012 ◽  
Vol 287 (31) ◽  
pp. 26312-26320 ◽  
Author(s):  
Xiu-Jun Zhang ◽  
Xiong-Zhuo Gao ◽  
Wei Yao ◽  
Rick H. Cote
Keyword(s):  
G Protein ◽  
Functional Mapping ◽  
Γ Subunit ◽  
Photoreceptor Phosphodiesterase

scholarly journals Mice with Deficiency of G Protein γ3 Are Lean and Have Seizures

2004 ◽  
Vol 24 (17) ◽  
pp. 7758-7768 ◽  
Author(s):  
William F. Schwindinger ◽  
Kathryn E. Giger ◽  
Kelly S. Betz ◽  
Anna M. Stauffer ◽  
Elaine M. Sunderlin ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
G Protein ◽  
Gene Targeting ◽  
Wild Type ◽  
Phenotypic Changes ◽  
Reduced Body ◽  
Γ Subunit ◽  
Body Weights ◽  
The Brain ◽  
Increased Susceptibility

ABSTRACT Emerging evidence suggests that the γ subunit composition of an individual G protein contributes to the specificity of the hundreds of known receptor signaling pathways. Among the twelve γ subtypes, γ3 is abundantly and widely expressed in the brain. To identify specific functions and associations for γ3, a gene-targeting approach was used to produce mice lacking the Gng3 gene (Gng3 −/−). Confirming the efficacy and specificity of gene targeting, Gng3 −/− mice show no detectable expression of the Gng3 gene, but expression of the divergently transcribed Bscl2 gene is not affected. Suggesting unique roles for γ3 in the brain, Gng3 −/− mice display increased susceptibility to seizures, reduced body weights, and decreased adiposity compared to their wild-type littermates. Predicting possible associations for γ3, these phenotypic changes are associated with significant reductions in β2 and αi3 subunit levels in certain regions of the brain. The finding that the Gng3 −/− mice and the previously reported Gng7 −/− mice display distinct phenotypes and different αβγ subunit associations supports the notion that even closely related γ subtypes, such as γ3 and γ7, perform unique functions in the context of the organism.

Stimulation by G Protein βγ Subunits of Phospholipase C β Isoforms in Human Platelets

1998 ◽  
Vol 79 (05) ◽  
pp. 1008-1013 ◽  
Author(s):  
Yoshiko Banno ◽  
Tomiko Asano ◽  
Yoshinori Nozawa
Keyword(s):  
Phospholipase C ◽  
G Protein ◽  
G Proteins ◽  
Human Platelet ◽  
Minor Component ◽  
Similar Efficacy ◽  
Human Platelets ◽  
Bovine Brain ◽  
Γ Subunit ◽  
A Minor

SummaryDifferent phospholipase C (PLC) isoforms were located in human platelet cytosol and membranes. PLCγ2 and PLCβ3b were mainly located in the cytosol and PLCβ2 and PLCβ3a were in both cytosol and membranes by using specific antibodies against PLC isozymes (Banno Y, Nakashima S, Ohzawa M, Nozawa Y. J Biol Chem 1996; 271: 14989-94). Three PLC fractions activated by G protein βγ subunits were purified from human platelet cytosol and membrane fractions. Two PLC fractions from membranes were identified as PLCβ2 and PLCβ3a, and one from cytosol was PLCβ3b. These PLCβ isoforms were activated by the purified βγ subunits of brain G proteins in the order PLCβ3b > PLCβ3a > PLCβ2. Western blot analysis of γ subunits of the purified platelet G proteins with antibodies against various standard γ subunits revealed that the major component of the γ subunit of Gi2 and Gq was γ5, and that γ7 was a minor component. Studies using various subtypes of βγ subunits, βγ2, βγ3, and βγ7 purified from bovine brain, βγ5 from bovine lung, or βγ12 from bovine spleen, failed to show differences in their ability to stimulate the isolated platelet PLCβ isoforms. These results suggest that the βγ subunits of Gi2 and Gq have similar efficacy in regulation of effectors in human platelets.

Identification of G‐protein binding sites of the human interleukin‐8 receptors by functional mapping of the intracellular loops

1996 ◽  
Vol 10 (12) ◽  
pp. 1426-1434 ◽  
Author(s):  
Bassam B. Damaj ◽  
Shaun R. McColl ◽  
Kuldeep Neote ◽  
Na Songqing ◽  
Kevin T. Ogborn ◽  
...  
Keyword(s):  
Protein Binding ◽  
G Protein ◽  
Binding Sites ◽  
Functional Mapping ◽  
Interleukin 8 ◽  
Protein Binding Sites ◽  
Intracellular Loops

scholarly journals GPC-1, a G Protein γ-Subunit, Regulates Olfactory Adaptation in Caenorhabditis elegans

Genetics ◽  
2009 ◽  
Vol 181 (4) ◽  
pp. 1347-1357 ◽  
Author(s):  
Koji Yamada ◽  
Takaaki Hirotsu ◽  
Masahiro Matsuki ◽  
Hirofumi Kunitomo ◽  
Yuichi Iino
Keyword(s):  
Caenorhabditis Elegans ◽  
G Protein ◽  
Γ Subunit ◽  
Olfactory Adaptation

The plant-specific G protein γ subunit AGG3 influences organ size and shape inArabidopsis thaliana

2012 ◽  
Vol 194 (3) ◽  
pp. 690-703 ◽  
Author(s):  
Shengjun Li ◽  
Yaju Liu ◽  
Leiying Zheng ◽  
Liangliang Chen ◽  
Na Li ◽  
...  
Keyword(s):  
G Protein ◽  
Organ Size ◽  
Size And Shape ◽  
Γ Subunit

scholarly journals A Novel Gγ Isolated fromDrosophilaConstitutes a Visual G Protein γ Subunit of the Fly Compound Eye

1999 ◽  
Vol 274 (53) ◽  
pp. 37605-37610 ◽  
Author(s):  
Simone Schulz ◽  
Armin Huber ◽  
Karin Schwab ◽  
Reinhard Paulsen
Keyword(s):  
G Protein ◽  
Compound Eye ◽  
Γ Subunit
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