Animal feeding stuffs: Methods of sampling and analysis - Detection and enumeration of Lactobacillus spp. used as feed additive

2021 ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 1511-1514
Author(s):  
Sergey Yu. Smolentsev ◽  
Galiya R Yusupova ◽  
Nikita V Nikolaev ◽  
Zaira F Aukhadieva ◽  
Renat A Volkov ◽  
...  

The effectiveness of dairy cattle breeding largely depends on the intensity of use of the breeding stock. At the same time, the factor of the duration of economic use of animals is gaining importance, which affects not only the production economy, but also the improvement of stocks and breeds. The lifelong productivity, the quantitative and qualitative growth of the stock, the amount of capital investment in its formation and the efficiency of operation depend on the term for the productive use of cows. The terms of breeding and production use of animals are direct linked with the biologically possible longevity of each animal, in turn, the duration of the productive period of cattle is in the range of 12-17 lactations. The influence of feed on production is associated primarily with the level of digestion and assimilation. The digestive capacity of the digestive tract of animals can be improved by selecting the optimal ratio in the diet of rough, juicy and concentrated feeds, i.e. optimization of animal feeding types. We conducted an analysis of milk productivity in lactating cows, when using AVMC feed additive in the diet. The experiment was conducted in the conditions of the dairy farm of Biryuli CJSC of the Vysokogorsky District of the Republic of Tatarstan on three groups of lactating cows. For the experiment, 30 cows were selected, 10 in each Holstein black-and-white breed according to the principle of analogues. Studies have shown that AVMC with rapeseed oil seeds and AVMC with fus in the diet of lactating cows provided higher animal productivity and positively affected the chemical composition of milk.


2018 ◽  
Vol 16 (1) ◽  
pp. 689-701 ◽  
Author(s):  
Katarzyna Godlewska ◽  
Krzysztof Marycz ◽  
Izabela Michalak

AbstractThe research was conducted to evaluate the possibility of using algae enriched with Cr(III) ions as a feed additive for horses. As a sorbent, macroalgaCladophora glomeratawas chosen. The results of the kinetic and equilibrium experiments on biosorption of Cr(III) ions are presented. The pseudo-second order model was used for the description of kinetics. Equilibrium of biosorption process was described by Langmuir model. The effect of biosorbent dose: 0.1–1.0 g·L-1, initial metal ions concentration: 100–300 mg·L-1and pH: 3–5 on the biosorption capacity in a batch system was evaluated. These factors played a significant role in affecting the biosorption capacity of biosorbent and the rate constant. Optimal pH for biosorption was 5, biosorbent dose 0.1 g·L-1, initial concentration of Cr(III) ions 300 mg·L-1. The maximum biosorption capacity determined from Langmuir equation was 107.5 mg·g-1(forCS1.0 g·L-1, pH 5). The experiments were also performed in a column system and they showed that almost 100% of Cr(III) ions were absorbed after 200 minutes. The FTIR and SEM-EDX technique confirmed binding of Cr(III) ions by the algal biomass. Due to very good biosorption properties,Cladophora glomeratacan be considered as a carrier of microelement ions in animal feeding.


2020 ◽  
Vol 8 (3) ◽  
pp. 322
Author(s):  
Eva-Maria Saliu ◽  
Hao Ren ◽  
Farshad Goodarzi Boroojeni ◽  
Jürgen Zentek ◽  
Wilfried Vahjen

Poultry frequently account for the highest prevalence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in livestock. To investigate the impact of direct-fed microbials (DFM) and phytobiotic feed additives on prevalence and conjugation of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae, an animal trial was conducted. Lactobacillus agilis LA73 and Lactobacillus salivarius LS1 and two commercial phytogenic feed additives (consisting of carvacrol, cinnamaldehyde, and eugenol) were used as feed additives either alone or as a combination of DFM and phytogenic feed additive. An ESBL-producing E. coli donor and a potentially pathogenic Salmonella Typhimurium recipient were inoculated at 5 × 109 cells/mL in cecal contents from 2-week-old broilers. Conjugation frequencies were determined after 4 h aerobic co-incubation at 37 °C and corrected for the impact of the sample matrix on bacterial growth of donor and recipient. Surprisingly, indigenous Enterobacteriaceae acted as recipients instead of the anticipated Salmonella recipient. The observed increase in conjugation frequency was most obvious in the groups fed the combinations of DFM and phytogenic product, but merely up to 0.6 log units. Further, cecal samples were examined for ESBL-producing Enterobacteriaceae on five consecutive days in broilers aged 27–31 days. All samples derived from animals fed the experimental diet showed lower ESBL-prevalence than the control. It is concluded that Lactobacillus spp. and essential oils may help to reduce the prevalence of ESBL-harboring plasmids in broilers, while the effect on horizontal gene transfer is less obvious.


2021 ◽  
Vol 33 ◽  
pp. 06012
Author(s):  
Dini Dwi Ludfiani ◽  
Widya Asmara ◽  
Agnesia Endang Tri Hastuti Wahyuni ◽  
Pudji Astuti

The use of antibiotic growth promoter as a feed additive for animals in Indonesia has been prohibited and regulated in law, starting from January 1, 2018. Alternative replacement for antibiotic was investigatedand introduced to farms such as probiotic. The commonly used probiotic was from the genus Lactobacillus, Bifidobacterium, and Bacillus. The aim of this study was to identify the Lactobacillus strains isolates from Jawa Super chicken excreta. Identification was based on the morphological, physiological, and biochemical characteristics. The sample was collected from Jawa Super chicken in Kalasan, Sleman, Yogyakarta. Each sample was diluted in 0.9% sterile saline and plated on MRS agar containing bromocresol purple. All plates were incubated at 37°C under anaerobic condition. Isolates were identified morphology, physiology, and biochemistry based on fermentation of carbohydrate by using Analytical Profile Index (API 50 CHL). This study showed that isolates belong to 2 categories of fermentation, facultatively heterofermentative and obligately heterofermentative. Not all isolates can grow at 10°C, but survive to low pH. Based on biochemical analysis isolates identify as L. brevis and L. pentosus. L. brevis and L. pentosus were strains of the Lactobacillus found in the excreta.


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