scholarly journals Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons

Water ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 66
Author(s):  
Rafaela Perdigão ◽  
C. Marisa R. Almeida ◽  
Filipa Santos ◽  
Maria F. Carvalho ◽  
Ana P. Mucha
Keyword(s):  
Petroleum Hydrocarbons ◽  
Oil Spills ◽  
Carbon Sources ◽  
Bacterial Biomass ◽  
Bacterial Consortium ◽  
Growth Conditions ◽  
Natural Seawater ◽  
Bacterial Strains ◽  
Beach Sediments ◽  
Insight Into

Oil spill pollution remains a serious concern in marine environments and the development of effective oil bioremediation techniques are vital. This work is aimed at developing an autochthonous hydrocarbon-degrading consortium with bacterial strains with high potential for hydrocarbons degradation, optimizing first the growth conditions for the consortium, and then testing its hydrocarbon-degrading performance in microcosm bioremediation experiments. Bacterial strains, previously isolated from a sediment and cryopreserved in a georeferenced microbial bank, belonged to the genera Pseudomonas, Rhodococcus and Acinetobacter. Microcosms were assembled with natural seawater and petroleum, for testing: natural attenuation (NA); biostimulation (BS) (nutrients addition); bioaugmentation with inoculum pre-grown in petroleum (BA/P) and bioaugmentation with inoculum pre-grown in acetate (BA/A). After 15 days, a clear blending of petroleum with seawater was observed in BS, BA/P and BA/A but not in NA. Acetate was the best substrate for consortium growth. BA/A showed the highest hydrocarbons degradation (66%). All bacterial strains added as inoculum were recovered at the end of the experiment. This study provides an insight into the capacity of autochthonous communities to degrade hydrocarbons and on the use of alternative carbon sources for bacterial biomass growth for the development of bioremediation products to respond to oil spills.

scholarly journals Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria

2021 ◽  
Vol 9 (11) ◽  
pp. 2285
Author(s):  
Rafaela Perdigão ◽  
C. Marisa R. Almeida ◽  
Catarina Magalhães ◽  
Sandra Ramos ◽  
Ana L. Carolas ◽  
...  
Keyword(s):  
Petroleum Hydrocarbons ◽  
Carbon Sources ◽  
Rhodococcus Erythropolis ◽  
Water Layer ◽  
Degradation Process ◽  
Bacterial Consortium ◽  
Total Petroleum Hydrocarbons ◽  
Natural Seawater ◽  
Bacterial Strains ◽  
Degrading Bacteria

This work aimed to develop a bioremediation product of lyophilized native bacteria to respond to marine oil spills. Three oil-degrading bacterial strains (two strains of Rhodococcus erythropolis and one Pseudomonas sp.), isolated from the NW Portuguese coast, were selected for lyophilization after biomass growth optimization (tested with alternative carbon sources). Results indicated that the bacterial strains remained viable after the lyophilization process, without losing their biodegradation potential. The biomass/petroleum ratio was optimized, and the bioremediation efficiency of the lyophilized bacterial consortium was tested in microcosms with natural seawater and petroleum. An acceleration of the natural oil degradation process was observed, with an increased abundance of oil-degraders after 24 h, an emulsion of the oil/water layer after 7 days, and an increased removal of total petroleum hydrocarbons (47%) after 15 days. This study provides an insight into the formulation and optimization of lyophilized bacterial agents for application in autochthonous oil bioremediation.

scholarly journals Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium

2020 ◽  
Vol 7 (2) ◽  
pp. 127-133
Author(s):  
Yalda Basim ◽  
Ghasemali Mohebali ◽  
Sahand Jorfi ◽  
Ramin Nabizadeh ◽  
Mehdi Ahmadi Moghadam ◽  
...  
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Environmental Concern ◽  
Bacterial Consortium ◽  
Oil Field ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Strains ◽  
Flame Ionization ◽  
And Control ◽  
Similar Texture

Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains present in the contaminated and non-contaminated soils were identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were determined using a gas chromatograph equipped with a flame ionization detector (GC-FID). Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae (84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial strains was less in oil-contaminated soils and varied significantly between case and control samples. Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is one of the innovative aspects of this research. Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency of 28.1% was obtained.

Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica

2020 ◽  
Vol 32 (5) ◽  
pp. 341-353 ◽  
Author(s):  
Mansur Abdulrasheed ◽  
Nur Nadhirah Zakaria ◽  
Ahmad Fareez Ahmad Roslee ◽  
Mohd Yunus Shukor ◽  
Azham Zulkharnain ◽  
...  
Keyword(s):  
Oil Spills ◽  
Growth Conditions ◽  
Diesel Oil ◽  
Bacterial Strains ◽  
Antarctic Soils ◽  
Cold Tolerant ◽  
Antarctic Treaty ◽  
Degradation Activity ◽  
The Antarctic ◽  
Diesel Degradation

AbstractBioremediation has been proposed as a means of dealing with oil spills on the continent. However, the introduction of non-native organisms, including microbes, even for this purpose would appear to breach the terms of the Environmental Protocol to the Antarctic Treaty. This study therefore aimed to optimize the growth conditions and diesel degradation activity of the Antarctic native bacteria Arthrobacter spp. strains AQ5-05 and AQ5-06 through the application of a one-factor-at-a-time (OFAT) approach. Both strains were psychrotolerant, with the optimum temperature supporting diesel degradation being 10–15°C. Both strains were also screened for biosurfactant production and biofilm formation. Their diesel degradation potential was assessed using Bushnell–Haas medium supplemented with 0.5% (v/v) diesel as the sole carbon source and determined using both gravimetric and gas chromatography and mass spectrophotometry analysis. Strain AQ5-06 achieved 37.5% diesel degradation, while strain AQ5-05 achieved 34.5% diesel degradation. Both strains produced biosurfactants and showed high biofilm adherence. Strains AQ5-05 and AQ5-06 showed high cellular hydrophobicity rates of 73.0% and 81.5%, respectively, in hexadecane, with somewhat lower values of 60.5% and 70.5%, respectively, in tetrahexadecane. Optimized conditions identified via OFAT increased diesel degradation to 41.0% and 47.5% for strains AQ5-05 and AQ5-06, respectively. Both strains also demonstrated the ability to degrade diesel in the presence of heavy metal co-pollutants. This study therefore confirms the potential use of these cold-tolerant bacterial strains in the biodegradation of diesel-polluted Antarctic soils at low environmental temperatures.

scholarly journals Biodegradation of Total Petroleum Hydrocarbons in Soil: Isolation and Characterization of Bacterial Strains from Oil Contaminated Soil

2020 ◽  
Vol 10 (12) ◽  
pp. 4173 ◽  
Author(s):  
Runkai Wang ◽  
Baichun Wu ◽  
Jin Zheng ◽  
Hongkun Chen ◽  
Pinhua Rao ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Ph Value ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Total Petroleum Hydrocarbons ◽  
Impact Factors ◽  
Growth Media ◽  
Bacterial Strains ◽  
Isolation And Characterization

In this study, we isolated seven strains (termed BY1–7) from polluted soil at an oil station and evaluated their abilities to degrade total petroleum hydrocarbons (TPHs). Following 16 rRNA sequence analysis, the strains were identified as belonging to the genera Bacillus, Acinetobacter, Sphingobium, Rhodococcus, and Pseudomonas, respectively. Growth characterization studies indicated that the optimal growth conditions for the majority of the strains was at 30 °C, with a pH value of approximately 7. Under these conditions, the strains showed a high TPH removal efficiency (50%) after incubation in beef extract peptone medium for seven days. Additionally, we investigated the effect of different growth media on growth impact factors that could potentially affect the strains’ biodegradation rates. Our results suggest a potential application for these strains to facilitate the biodegradation of TPH-contaminated soil.

scholarly journals In Vitro Study of Butyric Acid Deodorization Potential by Indigenously Constructed Bacterial Consortia and Pure Cultures from Pit Latrine Fecal Sludge

2020 ◽  
Vol 12 (12) ◽  
pp. 5156
Author(s):  
John Bright Joseph Njalam’mano ◽  
Evans Martin Nkhalambayausi Chirwa ◽  
Refilwe Lesego Seabi
Keyword(s):  
Butyric Acid ◽  
In Vitro Study ◽  
Bacterial Consortium ◽  
Growth Conditions ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
Fecal Sludge ◽  
Pure Cultures ◽  
Pit Latrine

The present study aims at developing an efficient bacterial consortium to biodegrade butyric acid, one of the odor-causing compounds that contribute significantly to pit latrine malodors. Six bacterial strains isolated from pit latrine fecal sludge were selected for the study. Nineteen bacterial consortia of different combinations were artificially constructed. The individual bacterial strains and bacterial consortia were compared by culturing in mineral salt medium supplemented with 1000 mg/L butyric acid as a sole carbon and energy source at pH 7, 30 °C, and 110 rpm under aerobic growth conditions. A co-culture of Serratia marcescens and Bacillus cereus was an effective bacterial consortium compared to individual component bacterial strains and other bacterial consortia, in which 1000 mg/L butyric acid was completely degraded within 16 h of incubation. A temperature of 30 °C and pH 7 were found to be optimum for the maximum degradation for both S. marcescens and B. cereus. The inoculation sizes of 2.0 and 2.5 were optimal for the maximum degradation for B. cereus and S. marcescens, respectively. The study provides insights that will be of substantial help in the development of effective biological treatment technologies for pit latrine odor to change the pit latrine user community’s and would be users’ perception of pit latrines.

Optimization of poly-β-hydroxybutyrate production by halotolerant bacterial strains isolated from saline environment

2016 ◽  
Vol 5 (08) ◽  
pp. 4775 ◽  
Author(s):  
Nabila Fathima ◽  
Veena Gayathri Krishnaswamy*
Keyword(s):  
Carbon Sources ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Bacterial Strains ◽  
Saline Environment ◽  
Carbon And Nitrogen ◽  
Nacl Concentration ◽  
Phb Production ◽  
Optimum Ph ◽  
Nitrogen Ratio

PHB is a biodegradable plastic which is becoming an environmentally friendly substitute to the synthetic plastics that are persistent and accumulate in large amounts and are non-degradable. PHB is a class of Polyhydroxyalkanoate which are similar to commercial plastics like polypropylene but with an added advantage of being biodegradable. To overcome the problem of commercializing PHB production by microorganisms because of the high cost involved, Halotolerant organisms can be used as they are easier to cultivate and do not require strict sterile conditions. In this present study PHB producing halotolerant bacterial strains were isolated from a marine environment and cultivated under saline conditions. The growth conditions of the bacterial strains were optimized for maximum production of PHB. The parameters such as pH, temperature, NaCl concentration, carbon sources, nitrogen sources and carbon and nitrogen ratio were optimized and studied.  The growth conditions for each of the parameter were optimized and the PHB production was estimated for the bacterial strains under saline conditions. The optimum pH and temperature range yielded maximum PHB production of about 42 – 45 mg/100ml and 30 – 40 mg/100ml respectively. The perspective application of PHB could be in the medicinal field for manufacturing medical devices as implants for various surgeries such as dental, cranio – maxillofacial and dental surgeries. 

scholarly journals BIOREMEDIATION OF CRUDE OIL CONTAMINATED SEAWATER WITH THE APPLICATION OF BIOSURFACTANT AND BIOSTIMULATION

2020 ◽  
Vol 41 (2) ◽  
pp. 109-115
Author(s):  
Syafrizal Syafrizal ◽  
Rendy Budi Prastiko ◽  
Tri Partono ◽  
Yanni Kussuryani
Keyword(s):  
Oil Content ◽  
Oil Spills ◽  
Carbon Sources ◽  
Bacterial Consortium ◽  
Marine Biota ◽  
Biodegradation Rate ◽  
The Media ◽  
Reduce Surface Tension ◽  
Marine Oil Spills ◽  
Hydrocarbon Compound

Marine oil spills have bad impacts on the marine biota. Oil spill mitigation that is currently safe, effi cient, relatively cheap and easy to implement is bioremediation, that is degradation of oil spills biologically using microorganisms. Petroleum will be more easily dispersed in water when surfactants are added. The surfactants have the ability to increase the bioavailability of petroleum to facilitate bacteria contact with carbon sources as their feed. This study was intended to test the effect of addition of diethanolamide (DEA) surfactants to improve the ability of bacteria to degrade hydrocarbon compound in the seawater media. The biodegradation experiment was conducted in 8-liter seawater media and the ability of DEA surfactants to reduce surface tension, oil content, pH and nutrients on days 0, 1, 3, 6 and 10 were observed. GC-MS analysis was conducted to detect chemical component changes in petroleum. A bacterial consortium of Enterobacter sp., Pseudomonas sp., and Raoultella sp. was utilized. The oil was degraded up to 65.52% with biodegradation rate k = -0.1054 t in the media added with DEA surfactants. The aliphatic fraction detected was C17-C31 n-alkane compound and after biodegradation it became C20- C31. The results showed that DEA surfactants were able to improve the ability of bacterial consortium to degrade petroleum.

scholarly journals Quantifying Microbial Utilization of Petroleum Hydrocarbons in Salt Marsh Sediments by Using the 13C Content of Bacterial rRNA

2007 ◽  
Vol 74 (4) ◽  
pp. 1157-1166 ◽  
Author(s):  
Ann Pearson ◽  
Kimberly S. Kraunz ◽  
Alex L. Sessions ◽  
Anne E. Dekas ◽  
William D. Leavitt ◽  
...  
Keyword(s):  
Salt Marsh ◽  
Petroleum Hydrocarbons ◽  
Oil Spills ◽  
Bacterial Biomass ◽  
Magnetic Bead ◽  
16S Rrna Genes ◽  
Microbial Consortia ◽  
Rrna Genes ◽  
Carbon Isotopic ◽  
Microbial Utilization

ABSTRACT Natural remediation of oil spills is catalyzed by complex microbial consortia. Here we took a whole-community approach to investigate bacterial incorporation of petroleum hydrocarbons from a simulated oil spill. We utilized the natural difference in carbon isotopic abundance between a salt marsh ecosystem supported by the 13C-enriched C4 grass Spartina alterniflora and 13C-depleted petroleum to monitor changes in the 13C content of biomass. Magnetic bead capture methods for selective recovery of bacterial RNA were used to monitor the 13C content of bacterial biomass during a 2-week experiment. The data show that by the end of the experiment, up to 26% of bacterial biomass was derived from consumption of the freshly spilled oil. The results contrast with the inertness of a nearby relict spill, which occurred in 1969 in West Falmouth, MA. Sequences of 16S rRNA genes from our experimental samples also were consistent with previous reports suggesting the importance of Gamma- and Deltaproteobacteria and Firmicutes in the remineralization of hydrocarbons. The magnetic bead capture approach makes it possible to quantify uptake of petroleum hydrocarbons by microbes in situ. Although employed here at the domain level, RNA capture procedures can be highly specific. The same strategy could be used with genus-level specificity, something which is not currently possible using the 13C content of biomarker lipids.

EFFECTS OF CHEMICAL DISPERSANTS AND MINERAL FINES ON PARTITIONING OF PETROLEUM HYDROCARBONS IN NATURAL SEAWATER

2008 ◽  
Vol 2008 (1) ◽  
pp. 633-638 ◽  
Author(s):  
Kenneth Lee ◽  
Zhengkai Li ◽  
Thomas King ◽  
Paul Kepkay ◽  
Michel C Boufadel ◽  
...  
Keyword(s):  
Crude Oil ◽  
Water Column ◽  
Petroleum Hydrocarbons ◽  
Oil Spills ◽  
Suspended Sediments ◽  
Bulk Water ◽  
Natural Seawater ◽  
Dispersed Oil ◽  
Aliphatic And Aromatic Hydrocarbons ◽  
Mineral Aggregates

ABSTRACT The interaction of chemical dispersants and suspended sediments with crude oil influences the fate and transport of oil spills in coastal waters. Recent wave tank studies have shown that dispersants facilitate the dissipation of oil droplets into the water column and reduces the particle size distribution of oil-mineral aggregates (OMAs). In this work, baffled flasks were used to carry out a controlled laboratory experimental study to define the effects of chemical dispersants and mineral fines on the partitioning of crude oil, major fractions of oil, and petroleum hydrocarbons from the surface to the bulk water column and the sediment phases. The dissolved and dispersed oil in the aqueous phase and OMA was characterized using an Ultraviolet Fluorescence Spectroscopy (UVFS). The distribution of major fractions of crude oil (the alkanes, aromatics, resins, and asphaltenes) was analyzed by thin layer chromatography coupled to flame ionized detection (TLC/FID); aliphatic and aromatic hydrocarbons were analyzed by gas chromatography and mass spectrometry (GC/MS). The results suggest that chemical dispersants enhanced the transfer of oil from the surface to the water column as dispersed oil, and promoted the formation of oil-mineral aggregates in the water column. Interaction of chemically dispersed oil with suspended particular materials needs to be considered in order to accurately assess the environmental risk associated with chemical oil dispersant use in particle-rich nearshore and esturine waters. The results from this study indicate that there is not necessarily an increase in sedimentation of oil in particle rich water when dispersants are applied.

scholarly journals EFFECT OF DIETANOLAMIDE (DEA) SURFACTANT ADDITION AND DEEP-SEA BACTERIA ACTIVITIES ON THE BIODEGRADABILITY OF ARTIFICIAL OILY WASTEWATER IN SEAWATER MEDIA

2020 ◽  
Vol 41 (2) ◽  
pp. 95-108
Author(s):  
Syafrizal Syafrizal ◽  
Rendy Budi Prastiko ◽  
Tri Partono ◽  
Yanni Kussuryani
Keyword(s):  
Oil Spills ◽  
Carbon Sources ◽  
Bacterial Consortium ◽  
Marine Biota ◽  
Oily Wastewater ◽  
Biodegradation Rate ◽  
Deep Sea Bacteria ◽  
The Media ◽  
Reduce Surface Tension ◽  
Hydrocarbon Compound

Marine oil spills have bad impacts on the marine biota. Oil spill mitigation that is currently safe, effi cient, relatively cheap and easy to implement is bioremediation, that is degradation of oil spills biologically using microorganisms. Petroleum will be more easily dispersed in water when surfactants are added. The surfactants have the ability to increase the bioavailability of petroleum to facilitate bacteria contact with carbon sources as their feed. This study was intended to test the effect of addition of diethanolamide (DEA) surfactants to improve the ability of bacteria to degrade hydrocarbon compound in the seawater media. The biodegradation experiment was conducted in 8-liter seawater media and the ability of DEA surfactants to reduce surface tension, oil content, pH and nutrients on days 0, 1, 3, 6 and 10 were observed. GC-MS analysis was conducted to detect chemical component changes in petroleum. A bacterial consortium of Enterobacter sp., Pseudomonas sp., and Raoultella sp. was utilized. The oil was degraded up to 65.52% with biodegradation rate k = -0.1054 t in the media added with DEA surfactants. The aliphatic fraction detected was C17-C31 n-alkane compound and after biodegradation it became C20- C31. The results showed that DEA surfactants were able to improve the ability of bacterial consortium to degrade petroleum.

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