scholarly journals Biodegradation of Total Petroleum Hydrocarbons in Soil: Isolation and Characterization of Bacterial Strains from Oil Contaminated Soil

2020 ◽  
Vol 10 (12) ◽  
pp. 4173 ◽  
Author(s):  
Runkai Wang ◽  
Baichun Wu ◽  
Jin Zheng ◽  
Hongkun Chen ◽  
Pinhua Rao ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Ph Value ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Total Petroleum Hydrocarbons ◽  
Impact Factors ◽  
Growth Media ◽  
Bacterial Strains ◽  
Isolation And Characterization

In this study, we isolated seven strains (termed BY1–7) from polluted soil at an oil station and evaluated their abilities to degrade total petroleum hydrocarbons (TPHs). Following 16 rRNA sequence analysis, the strains were identified as belonging to the genera Bacillus, Acinetobacter, Sphingobium, Rhodococcus, and Pseudomonas, respectively. Growth characterization studies indicated that the optimal growth conditions for the majority of the strains was at 30 °C, with a pH value of approximately 7. Under these conditions, the strains showed a high TPH removal efficiency (50%) after incubation in beef extract peptone medium for seven days. Additionally, we investigated the effect of different growth media on growth impact factors that could potentially affect the strains’ biodegradation rates. Our results suggest a potential application for these strains to facilitate the biodegradation of TPH-contaminated soil.

scholarly journals Bioremediation of Petroleum Hydrocarbons Contaminated Soil using Bio piles System

2019 ◽  
Vol 16 (1) ◽  
pp. 0185 ◽  
Author(s):  
Jabbar Et al.
Keyword(s):  
Surface Tension ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Bacterial Culture ◽  
Pilot Scale ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Strains ◽  
Mixed Bacterial Culture ◽  
Surface Tension Reduction

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. Four bacterial strains were isolated from diesel contaminated soil samples. The isolates were identified by the Vitek 2 system, as Sphingomonas paucimobilis, Pentoae species, Staphylococcus aureus, and Enterobacter cloacae. The potential of biological surfactant production was tested using the Sigma 703D stand-alone tensiometer showed that these isolates are biological surfactant producers. The better results of the surface tension reduction test were obtained using the mixed bacterial culture which reduced the surface tension of the medium from 66mN/m to 33.89mN/m. For further evidence of the biodegradation effect of these isolates individually and as a mixed culture, which was supported by the use of Gas-Chromatography technology confirming the occurrence of biodegradation. The capability of mixed bacterial culture was examined to remediate the diesel contaminated soil in bio piles system. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). Both systems were equipped with oxygen to provide aerobic conditions, incubated at ambient temperature and weekly sampling within 35 days (during summer season). Overall 75.71 % of the total petroleum hydrocarbons were removed from the amended soil and 33.18 % of the control soil at the end of study period. The study concluded that the ex-situ bioremediation (bio piles) is a good option for treating the soil contaminated with diesel as economical and environmentally friendly.

Bioremediation of Petroleum Hydrocarbons Contaminated Soil using Bio piles System

2019 ◽  
Vol 16 (1(Suppl.)) ◽  
pp. 0185
Author(s):  
Jabbar Et al.
Keyword(s):  
Surface Tension ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Bacterial Culture ◽  
Pilot Scale ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Strains ◽  
Mixed Bacterial Culture ◽  
Surface Tension Reduction

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. Four bacterial strains were isolated from diesel contaminated soil samples. The isolates were identified by the Vitek 2 system, as Sphingomonas paucimobilis, Pentoae species, Staphylococcus aureus, and Enterobacter cloacae. The potential of biological surfactant production was tested using the Sigma 703D stand-alone tensiometer showed that these isolates are biological surfactant producers. The better results of the surface tension reduction test were obtained using the mixed bacterial culture which reduced the surface tension of the medium from 66mN/m to 33.89mN/m. For further evidence of the biodegradation effect of these isolates individually and as a mixed culture, which was supported by the use of Gas-Chromatography technology confirming the occurrence of biodegradation. The capability of mixed bacterial culture was examined to remediate the diesel contaminated soil in bio piles system. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). Both systems were equipped with oxygen to provide aerobic conditions, incubated at ambient temperature and weekly sampling within 35 days (during summer season). Overall 75.71 % of the total petroleum hydrocarbons were removed from the amended soil and 33.18 % of the control soil at the end of study period. The study concluded that the ex-situ bioremediation (bio piles) is a good option for treating the soil contaminated with diesel as economical and environmentally friendly.

Effect of compost in phytoremediation of diesel-contaminated soils

2001 ◽  
Vol 43 (2) ◽  
pp. 291-295 ◽  
Author(s):  
J. Vouillamoz ◽  
M. W. Milke
Keyword(s):  
Moisture Content ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Dilution Effect ◽  
Screening Tests ◽  
Total Petroleum Hydrocarbons ◽  
Controlled Environment ◽  
Grass Growth ◽  
The Impact

The effect of compost on phytoremediation of diesel-contaminated soils was investigated using 130 small (200 g) containers in two screening tests. The experiments were conducted in a controlled environment using ryegrass from seed. Containers were destructively sampled at various times and analyzed for plant mass and total petroleum hydrocarbons. The results indicate that the presence of diesel reduces grass growth, and that compost helps reduced the impact of diesel on grass growth. The addition of compost helps increase diesel loss from the soils both with and without grass, though the addition of grass leads to lower diesel levels compared with controls. A second set of experiments indicates that the compost helps in phytoremediation of diesel-contaminated soil independent of the dilution effect that compost addition has. The results indicate that the compost addition allowed diesel loss down to 200 mg TPH/kg even though the compost would be expected to hold the diesel more tightly in the soil/compost mixture. The simplicity of the screening tests led to difficulties in controlling moisture content and germination rates. The conclusion of the research is that the tilling of compost into soils combined with grass seeding appears to be a valuable option for treating petroleum-contaminated soils.

scholarly journals Soil Microcosms for Bioaugmentation With Fungal Isolates to Remediate Petroleum Hydrocarbon-contaminated Soil

2021 ◽  
Author(s):  
Dalel Daâssi ◽  
Fatimah Qabil Almaghribi
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Fungal Growth ◽  
Total Petroleum Hydrocarbons ◽  
Fungal Culture ◽  
Soil Microcosms ◽  
Fungal Isolates ◽  
Petroleum Contaminated Soil ◽  
Decaying Wood ◽  
Native Strains

Abstract The aim of this work was to isolate indigenous PAH degrading-fungi from petroleum contaminated soil and exogenous ligninolytic strains from decaying-wood, with the ability to secrete diverse enzyme activity. A total of ten ligninolytic fungal isolates and two native strains, has been successfully isolated, screened and identified. The phylogenetic analysis revealed that the indigenous fungi (KBR1 and KB8) belong to the genus Aspergillus niger and tubingensis. While the ligninolytic exogenous PAH-degrading strains namely KBR1-1, KB4, KB2 and LB3 were affiliated to different genera like Syncephalastrum sp, Paecilomyces formosus, Fusarium chlamydosporum, and Coniochaeta sp., respectively. Basis on the taxonomic analysis, enzymatic activities and the hydrocarbons removal rates, single fungal culture employing the strain LB3, KB4, KBR1 and the mixed culture (LB3+KB4) were selected to be used in soil microcosms treatments. The Total petroleum hydrocarbons (TPH), fungal growth rates, BOD5/COD ratios and GC-MS analysis, were determined in all soil microcosmos treatments (SMT) and compared with those of the control (SMU). After 60 days of culture incubation, the highest rate of TPH degradation was recorded in SMT[KB4] by approximately 92±2.35% followed by SMT[KBR1] then SMT[LB3+KB4] with 86.66±1.83% and 85.14±2.21%, respectively.

scholarly journals USE OF THE BIOSTIMULATION OF INDIGENOUS MICROBIAL COMMUNITIES TO DEGRADE TOTAL PETROLEUM HYDROCARBONS IN CONTAMINATED SOIL

2019 ◽  
Vol 17 (3) ◽  
Author(s):  
E J GUTIÉRREZ-ALCÁNTARA ◽  
D TIRADO-TORRES ◽  
G VÁZQUEZ-RODRÍGUEZ ◽  
E DELGADILLO-RUÍZ ◽  
M SALAZAR-HERNÁNDEZ ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Total Petroleum Hydrocarbons

Hydrocarbon biodegradation in oxygen-limited sequential batch reactors by consortium from weathered, oil-contaminated soil

2005 ◽  
Vol 51 (3) ◽  
pp. 231-239 ◽  
Author(s):  
S A Medina-Moreno ◽  
S Huerta-Ochoa ◽  
M Gutiérrez-Rojas
Keyword(s):  
Contaminated Soil ◽  
Batch Reactor ◽  
Oxygen Limitation ◽  
Total Petroleum Hydrocarbons ◽  
Hydrocarbon Biodegradation ◽  
Batch Reactors ◽  
Native Plant ◽  
Low Oxygen ◽  
Bacterial Strains ◽  
Transfer Coefficients

We studied the use of sequential batch reactors under oxygen limitation to improve and maintain consortium ability to biodegrade hydrocarbons. Air-agitated tubular reactors (2.5 L) were operated for 20 sequential 21-day cycles. Maya crude oil – paraffin mixture (13 000 mg/L) was used as the sole carbon source. The reactors were inoculated with a consortium from the rhizosphere of Cyperus laxus, a native plant that grows naturally in weathered, contaminated soil. Oxygen limitation was induced in the tubular reactor by maintaining low oxygen transfer coefficients (kLa < 20.6 h–1). The extent and biodegradation rates increased significantly up to the fourth cycle, maintaining values of about 66.33% and 460 mg·L–1·d–1, respectively. Thereafter, sequential batch reactor operation exhibited a pattern with a constant general trend of biodegradation. The effect of oxygen limitation on consortium activity led to a low biomass yield and non-soluble metabolite (0.45 g SS/g hydrocarbons consumed). The average number of hydrocarbon-degrading microorganisms increased from 6.5 × 107 (cycles 1–3) to 2.2 × 108 (cycles 4–20). Five bacterial strains were identified: Achromobacter (Alcaligenes) xylosoxidans, Bacillus cereus, Bacillus subtilis, Brevibacterium luteum, and Pseudomonas pseudoalcaligenes. Asphaltene-free total petroleum hydrocarbons, extracted from a weathered, contaminated soil, were also biodegraded (97.1 mg·L–1·d–1) and mineralized (210.48 mg CO2·L–1·d–1) by the enriched consortium without inhibition. Our results indicate that sequential batch reactors under oxygen limitation can be used to produce consortia with high and constant biodegradation ability for industrial applications of bioremediation.Key words: sequential batch reactors, oxygen limitation, consortium, hydrocarbon biodegradation.

Production of Novel Exopolysaccharide with Emulsifying Ability from Marine Microorganism, Alteromonas sp. Strain 00SS11568

2005 ◽  
Vol 277-279 ◽  
pp. 155-161 ◽  
Author(s):  
Joung Han Yim ◽  
Se Hun Ahn ◽  
Sung Jin Kim ◽  
Yoo Kyung Lee ◽  
Kyu Jin Park ◽  
...  
Keyword(s):  
Inorganic Compounds ◽  
Gel Filtration ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Biochemical Properties ◽  
Medium Composition ◽  
Molar Ratio ◽  
Bacterial Strains ◽  
Average Molecular Mass ◽  
Emulsifying Ability

To find a novel exopolysaccharide, marine bacterial strains were isolated from coastal regions of Korea. Strain 00SS11568 was then selected as it produced a mucous exopolysaccharide during the stationary phase in a batch culture. The isolate was identified as Alteromonas sp. based on its 16S rDNA sequence, morphological, and biochemical properties. The exopolysaccharide, designated as p-11568, exhibited an emulsifying ability. The Emulsification Index (E24) of 0.1% p- 11568 was 77.4% with an emulsified kerosene content, and was higher than those of commercial polysaccharides, such as xanthan gum (26.1%), gellan gum (1.3%), and sodium alginate (2.0%). p- 11568 was found to be composed of glucose and galactose as the main natural sugars in a molar ratio of 1.3:1, along with uronic acid (18.9%, w/w) and sulfate groups (1.2% w/w). The average molecular mass was 4.4 x 105 daltons by gel filtration chromatography. The effects of pH, temperature, inorganic compounds, and C and N sources were tested to obtain the optimal medium composition for the production of p-11568. Under optimal growth conditions with the M-11568 medium, 14.9 g of crude p-11568 per liter was obtained.

Optimal growth temperature for the isolation of Plesiomonas shigelloides, using various selective and differential agars

1991 ◽  
Vol 37 (10) ◽  
pp. 800-802 ◽  
Author(s):  
Anwarul Huq ◽  
Anwari Akhtar ◽  
M. A. R. Chowdhury ◽  
David A. Sack
Keyword(s):  
Growth Temperature ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Aeromonas Species ◽  
Growth Media ◽  
Optimal Growth Temperature ◽  
Environmental Waters ◽  
Temperature Growth ◽  
Plesiomonas Shigelloides ◽  
Incubation Temperatures

The growth characteristics of known strains of Plesiomonas shigelloides were compared with those of Aeromonas species (the major competing species in environmental waters) on plesiomonas differential agar, inositol brilliant green bile salt, and modified salmonella–shigella agar at incubation temperatures of 37, 42, and 44 °C. Using local isolates from clinical and environmental sources, optimal growth conditions, as determined by colony counts and the colony characteristics, plesiomonas differential agar proved to be ideal when incubated at 44 °C. Contrary to earlier recommendations for 48 h incubation, the colonies could be recognized readily after an incubation of 24 h. Key words: Plesiomonas, growth temperature, growth media.

Isolation and Characterization of Rhodopseudomonas sp. S9-1 Capable of Degrading Pyrazosulfuron-Ethyl

2011 ◽  
Vol 356-360 ◽  
pp. 1152-1163 ◽  
Author(s):  
Le Bin Yin ◽  
Yong Liu ◽  
De Yong Zhang ◽  
Song Bai Zhang
Keyword(s):  
Contaminated Soil ◽  
Acetolactate Synthase ◽  
Optimal Growth ◽  
Degradation Process ◽  
Amino Acid Sequences ◽  
Rrna Gene ◽  
Photosynthetic Membrane ◽  
Physiological And Biochemical Characteristics ◽  
Isolation And Characterization

A bacterial strain S9-1capable of degrading sulfonylurea herbicide pyrazosulfuron-ethyl (PSE) was isolated from contaminated soil through the enrichment incubation method. Based on morphology, colony and cultural properties, physiological and biochemical characteristics, living-cell absorption spectra, internal photosynthetic membrane, and phylogenetics of its 16S rRNA gene sequence, S9-1was preliminarily identified as belonging to the genus Rhodopseudomonas, a group of photosynthetic bacteria (PSB). The effects of PSE concentration, pH, and temperature on biodegradation were examined. The degradation rate was found to decrease with increasing PSE concentration. Optimal growth pH and temperature were found to be 7.0 and 30°C, respectively. The strain was able to degrade 47.51% of PSE at a concentration of 100 mg ml-1after 7 days of incubation at 30°C and could tolerate 800 mg ml-1PSE. S9-1was also able to completely co-metabolically transform 100 mg ml-1PSE at 30°C, pH 7.0, and 7500 lux in 15 days. As the concentration of PSE increased, the degradation process took longer to complete. The fragment encoding acetolactate synthase (ALS) gene from S9-1was cloned and sequenced. Comparison of deduced amino acid sequences was implemented, and the conserved sites were analyzed. To our knowledge, this is the first report of PSB in PSE biodegradation. These results highlight the potential of this bacterium as a detoxifying agent for use with PSE-contaminated soil and wastewater.

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