scholarly journals Complex Virus–Host Interactions Involved in the Regulation of Classical Swine Fever Virus Replication: A Minireview

Viruses ◽  
2017 ◽  
Vol 9 (7) ◽  
pp. 171 ◽  
Author(s):  
Su Li ◽  
Jinghan Wang ◽  
Qian Yang ◽  
Muhammad Naveed Anwar ◽  
Shaoxiong Yu ◽  
...  
Keyword(s):  
Virus Replication ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Host Interactions

Analysis of classical swine fever virus replication kinetics allows differentiation of highly virulent from avirulent strains

2000 ◽  
Vol 74 (4) ◽  
pp. 293-308 ◽  
Author(s):  
Christian Mittelholzer ◽  
Christian Moser ◽  
Jon-Duri Tratschin ◽  
Martin A Hofmann
Keyword(s):  
Virus Replication ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Highly Virulent

scholarly journals Swine Host Protein Coiled-Coil Domain-Containing 115 (CCDC115) Interacts with Classical Swine Fever Virus Structural Glycoprotein E2 during Virus Replication

Viruses ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 388 ◽  
Author(s):  
Elizabeth A. Vuono ◽  
Elizabeth Ramirez-Medina ◽  
Keith Berggren ◽  
Ayushi Rai ◽  
Sarah Pruitt ◽  
...  
Keyword(s):  
Amino Acid ◽  
Virus Replication ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Host Protein ◽  
Yeast Two Hybrid ◽  
Host Proteins ◽  
E2 Protein ◽  
Two Hybrid

Interactions between the major structural glycoprotein E2 of classical swine fever virus (CSFV) with host proteins have been identified as important factors affecting virus replication and virulence. Previously, using the yeast two-hybrid system, we identified swine host proteins specifically interacting with CSFV E2. In this report, we use a proximity ligation assay to demonstrate that swine host protein CCDC115 interacts with E2 in CSFV-infected swine cells. Using a randomly mutated E2 library in the context of a yeast two-hybrid methodology, specific amino acid mutations in the CSFV E2 protein responsible for disrupting the interaction with CCDC115 were identified. A recombinant CSFV mutant (E2ΔCCDC115v) harboring amino acid changes disrupting the E2 protein interaction with CCDC115 was produced and used as a tool to assess the role of the E2–CCDC115 interaction in viral replication and virulence in swine. CSFV E2ΔCCDC115v showed a slightly decreased ability to replicate in the SK6 swine cell line and a greater replication defect in primary swine macrophage cultures. A decreased E2–CCDC115 interaction detected by PLA is observed in cells infected with E2ΔCCDC115v. Importantly, animals intranasally infected with 105 TCID50 of E2ΔCCDC115v experienced a significantly longer survival period when compared with those infected with the parental Brescia strain. This result would indicate that the ability of CSFV E2 to bind host CCDC115 protein during infection plays an important role in virus replication in swine macrophages and in virus virulence during the infection in domestic swine.

Curcumin inhibits classical swine fever virus replication by interfering with lipid metabolism

2021 ◽  
pp. 109152
Author(s):  
Ya Gao ◽  
Jia-Huan Hu ◽  
Xiao-Dong Liang ◽  
Jing Chen ◽  
Chun-Chun Liu ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Virus Replication ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus

In vitro inhibition of classical swine fever virus replication by siRNAs targeting Npro and NS5B genes

2008 ◽  
Vol 78 (3) ◽  
pp. 188-193 ◽  
Author(s):  
Xingran Xu ◽  
Huancheng Guo ◽  
Chang Xiao ◽  
Yunfeng Zha ◽  
Zixue Shi ◽  
...  
Keyword(s):  
Virus Replication ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
In Vitro Inhibition
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