scholarly journals Complete Sequence, Analysis and Organization of the Orgyia leucostigma Nucleopolyhedrovirus Genome

Viruses ◽  
2011 ◽  
Vol 3 (11) ◽  
pp. 2301-2327 ◽  
Author(s):  
David K. Thumbi ◽  
Robert J. M. Eveleigh ◽  
Christopher J. Lucarotti ◽  
Renée Lapointe ◽  
Robert I. Graham ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Complete Sequence ◽  
Orgyia Leucostigma

scholarly journals Genetic Mechanisms behind the Spread of Reduced Susceptibility to Azithromycin in Shigella Strains Isolated from Men Who Have Sex with Men in Québec, Canada

2018 ◽  
Vol 63 (2) ◽  
pp. e01679-18 ◽  
Author(s):  
Khadidja Yousfi ◽  
Christiane Gaudreau ◽  
Pierre A. Pilon ◽  
Brigitte Lefebvre ◽  
Matthew Walker ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Shigella Flexneri ◽  
Complete Sequence ◽  
Shigella Sonnei ◽  
Content Type ◽  
Genetic Mechanisms ◽  
Sex With Men

ABSTRACT We analyzed 254 Shigella species isolates collected in Québec, Canada, during 2013 and 2014. Overall, 23.6% of isolates showed reduced susceptibility to azithromycin (RSA) encoded by mphA (11.6%), ermB (1.7%), or both genes (86.7%). Shigella strains with RSA were mostly isolated from men who have sex with men (68.8% or higher) from the Montreal region. A complete sequence analysis of six selected plasmids from Shigella sonnei and different serotypes of Shigella flexneri emphasized the role of IS26 in the dissemination of RSA.

scholarly journals Sequence Analysis of Inducible Prophage phIS3501 Integrated into the Haemolysin II Gene of Bacillus thuringiensis var israelensis ATCC35646

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Bouziane Moumen ◽  
Christophe Nguen-The ◽  
Alexei Sorokin
Keyword(s):  
Staphylococcus Aureus ◽  
Sequence Analysis ◽  
Bacillus Thuringiensis ◽  
Bacillus Cereus ◽  
Genomic Organization ◽  
Food Poisoning ◽  
Complete Sequence ◽  
Bacterial Host ◽  
Regulation Of Synthesis ◽  
Selection For

Diarrheic food poisoning by bacteria of the Bacillus cereus group is mostly due to several toxins encoded in the genomes. One of them, cytotoxin K, was recently identified as responsible for severe necrotic syndromes. Cytotoxin K is similar to a class of proteins encoded by genes usually annotated as haemolysin II (hlyII) in the majority of genomes of the B. cereus group. The partially sequenced genome of Bacillus thuringiensis var israelensis ATCC35646 contains several potentially induced prophages, one of them integrated into the hlyII gene. We determined the complete sequence and established the genomic organization of this prophage-designated phIS3501. During induction of excision of this prophage with mitomycin C, intact hlyII gene is formed, thus providing to cells a genetic ability to synthesize the active toxin. Therefore, this prophage, upon its excision, can be implicated in the regulation of synthesis of the active toxin and thus in the virulence of bacterial host. A generality of selection for such systems in bacterial pathogens is indicated by the similarity of this genetic arrangement to that of Staphylococcus aureus  β-haemolysin.

Complete sequence analysis reveals two distinct poleroviruses infecting cucurbits in China

2008 ◽  
Vol 153 (6) ◽  
pp. 1155-1160 ◽  
Author(s):  
Hai-ying Xiang ◽  
Qiao-xia Shang ◽  
Cheng-gui Han ◽  
Da-wei Li ◽  
Jia-lin Yu
Keyword(s):  
Sequence Analysis ◽  
Complete Sequence

scholarly journals Complete sequence analysis of mitochondrial DNA and telomere length in aplastic anemia

2014 ◽  
Vol 34 (5) ◽  
pp. 1309-1314 ◽  
Author(s):  
XING CUI ◽  
JUNQIANG WANG ◽  
ZHIGUO CAI ◽  
JINGYI WANG ◽  
KUI LIU ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Sequence Analysis ◽  
Aplastic Anemia ◽  
Telomere Length ◽  
Complete Sequence

Differences in cultivar response and complete sequence analysis of two isolates of wheat yellow mosaic bymovirus in China

2000 ◽  
Vol 49 (3) ◽  
pp. 370-374 ◽  
Author(s):  
J. Chen ◽  
J.-P. Chen ◽  
J.-P. Yang ◽  
Y. Cheng ◽  
A. Diao ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Complete Sequence

scholarly journals Purification of enzymes of the kallikrein gene family (rK8 and rK9) from the rat prostate

1994 ◽  
Vol 302 (1) ◽  
pp. 229-235 ◽  
Author(s):  
H Schøyen ◽  
I Wassdal ◽  
K Toft ◽  
M Almendingen ◽  
T Berg
Keyword(s):  
Sequence Analysis ◽  
Submandibular Gland ◽  
Serine Proteases ◽  
Biochemical Characterization ◽  
Sequence Similarity ◽  
Complete Sequence ◽  
Polyclonal Antiserum ◽  
Bean Trypsin Inhibitor ◽  
Rat Prostate ◽  
Ph Range

The rat kallikrein family consists of multiple closely related proteins. A method for demonstration and identification of kallikrein-like proteins has been developed based on their differences in isoelectric point and their immunological similarity. The method, which involved separation in flat-bed isoelectro-focusing gels (pH range 3-9) and detection by immunoblotting using polyclonal antiserum against one of the family members, has been used in the present study to detect kallikrein-like proteins in the rat prostate. Nine immunoreactive kallikrein-like protein bands were detected with pI ranging from 5.30 to 8.35. Of these, six were completely purified and three were partially purified. Two proteins (pI 5.30 and 6.75-6.90) corresponded to protein bands in gels of rat submandibular-gland extracts, and were identified by partial amino acid sequence analysis as rK8 and rK9 respectively. In addition, sequence analysis revealed complete sequence similarity between rK9 and the immunoreactive prostate proteins with pI 7.15, 7.25, 7.50 and 8.27. On the basis of this finding and immunological and biochemical characterization, we concluded that all the kallikrein-like proteins detected, except for rK8, represented isoenzymes of rK9. The molecular masses of the prostate rK9 isoenzymes (24,600-29,300 Da) were close to that of submandibular-gland rK9 (24,600 Da), although differences were observed after reduction with mercaptoethanol. The prostate rK9 isoenzymes were, like submandibular-gland rK9, inhibited by soya-bean trypsin inhibitor but not by aprotinin, and were classified as serine proteases as they were inhibited by phenylmethanesulphonyl fluoride. rK8 (28,700 Da) showed no activity with any of the substrates tested, and its inhibitory profile could therefore not be studied. No other enzymes of the kallikrein family were found in the rat prostate.

scholarly journals Molecular and Evolutionary Characterization of the cp32/18 Family of Supercoiled Plasmids in Borrelia burgdorferi297

2000 ◽  
Vol 68 (3) ◽  
pp. 1574-1586 ◽  
Author(s):  
Melissa J. Caimano ◽  
Xiaofeng Yang ◽  
Taissia G. Popova ◽  
Michael L. Clawson ◽  
Darrin R. Akins ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Genomic Sequence ◽  
Complete Sequence ◽  
Open Reading Frames ◽  
Exogenous Dna ◽  
Variable Regions ◽  
Different Types ◽  
Sequence Relatedness ◽  
Reading Frames

ABSTRACT In this study, we characterized seven members of the cp32/18 family of supercoiled plasmids in Borrelia burgdorferi297. Complete sequence analysis of a 21-kb plasmid (cp18-2) confirmed that the strain 297 plasmids are similar in overall content and organization to their B31 counterparts. Of the 31 open reading frames (ORFs) in cp18-2, only three showed sequence relatedness to proteins with known functions, and only one, a ParA/SopA ortholog, was related to nonborrelial polypeptides. Besides the lipoproteins, none of the ORFs appeared likely to encode a surface-exposed protein. Comparison with the B31 genomic sequence indicated that paralogs for most of the ORFs in cp18-2 can be identified on other genetic elements. cp18-2 was found to lack a 9- to 10-kb fragment present in the 32-kb homologs which, by extrapolation from the B31 cp32 sequences, contains at least 15 genes presumed to be unnecessary for plasmid maintenance. Sequence analysis of the lipoprotein-encoding variable loci provided evidence that recombinatorial processes within these regions may result in the acquisition of exogenous DNA. Pairwise analysis with random shuffling revealed that the multiple lipoproteins (Mlp; formerly designated 2.9 LPs) fall into two distinct homology groups which appear to have arisen by gene fusion events similar to those recently proposed to have generated the three OspE, OspF, and Elp lipoprotein families (D. R. Akins, M. J. Caimano, X. Yang, F. Cerna, M. V. Norgard, and J. D. Radolf, Infect. Immun. 67:1526–1532, 1999). Comparative analysis of the variable regions also indicated that recombination within the loci of each plasmid may occur independently. Last, comparison of variable loci revealed that the cp32/18 plasmid complements of the B31 and 297 isolates differ substantially, indicating that the two strains have been subject to divergent adaptive pressures. In addition to providing evidence for two different types of recombinatorial events involving cp32/18 plasmids, these findings underscore the need for genetic analysis of diverse borrelial isolates in order to elucidate the Lyme disease spirochete's complex parasitic strategies.

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