scholarly journals A Design of Experiment Approach to Optimize Spray-Dried Powders Containing Pseudomonas aeruginosaPodoviridae and Myoviridae Bacteriophages

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1926
Author(s):  
Emilie Tabare ◽  
Tea Glonti ◽  
Christel Cochez ◽  
Cyrille Ngassam ◽  
Jean-Paul Pirnay ◽  
...  
Keyword(s):  
Spray Drying ◽  
Flow Rate ◽  
Gas Flow ◽  
Negative Impact ◽  
Positive Impact ◽  
Inlet Temperature ◽  
Spray Dryer ◽  
Gas Flow Rate ◽  
Residual Moisture ◽  
Spray Dried

In the present study, we evaluated the effect of spray-drying formulations and operating parameters of a laboratory-scale spray-dryer on the characteristics of spray-dried powders containing two Pseudomonas aeruginosa bacteriophages exhibiting different morphotypes: a podovirus (LUZ19) and a myovirus (14-1). We optimized the production process for bacteriophage-loaded powders, with an emphasis on long-term storage under ICH (international conference on harmonization) conditions. D-trehalose-/L-isoleucine-containing bacteriophage mixtures were spray-dried from aqueous solutions using a Büchi Mini Spray-dryer B-290 (Flawil, Switzerland). A response surface methodology was used for the optimization of the spray-drying process, with the following as-evaluated parameters: Inlet temperature, spray gas flow rate, and the D-trehalose/L-isoleucine ratio. The dried powders were characterized in terms of yield, residual moisture content, and bacteriophage lytic activity. L-isoleucine has demonstrated a positive impact on the activity of LUZ19, but a negative impact on 14-1. We observed a negligible impact of the inlet temperature and a positive correlation of the spray gas flow rate with bacteriophage activity. After optimization, we were able to obtain dry powder preparations of both bacteriophages, which were stable for a minimum of one year under different ICH storage conditions (up to and including 40 °C and 75% relative humidity).

scholarly journals Optimization of Spray Drying Condition of Asparagus Powder Using Response Surface Methodology (P17-003-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Jiseon Park ◽  
Soon Bae Kwon ◽  
Hye Jeong Kwon
Keyword(s):  
Spray Drying ◽  
Flow Rate ◽  
Air Flow ◽  
Absorption Index ◽  
Water Extraction ◽  
Inlet Temperature ◽  
Air Flow Rate ◽  
Independent Variables ◽  
Drying Conditions ◽  
Spray Dried

Abstract Objectives The purpose of this study was to investigate optimization of spray drying conditions for water-soluble powder using response surface methodology that is a statistical procedure used for optimization studies. Methods First, conditions of the extract used for spray drying were set. We compared heat water extraction (60–100 °C) with ethanol extraction (10–50%). After final selection of the method of extract used for spray drying, spray drying conditions were set. Independent variables included the additive contents of maltodextrin (X1), inlet temperature (X2), and air flow rate (X3). The dependent variables were yield, water absorption index (WAI) and total phenolic compounds. Results The yield was highest in 100 °C heat water extraction. The content of rutin was 29.77 mg/100 g in 90 °C heat water extraction, 28.07 mg/100 g in 100 °C heat water extraction and 24.24 mg/100 g in 10% ethanol extraction. The heat water extraction method at 100 °C was selected as an extract of the spray dryer. Statistical analysis revealed that independent variables significantly affected all the responses. A maximum yield was obtained at 15.55% of X1, 167.87 °C of X2 and 50.00 mL/min of X3. The water absorption index of asparagus increased with increasing MD ratio (X1), higher inlet temperature (X2) and higher air flow rate (X3). The total polyphenol contents of asparagus were higher when the MD addition ratio (X1) was 16.56%, the inlet temperature (X2) was higher and the air flow rate (X3) was higher. Conclusions In this study, extracts of asparagus using different extraction methods were compared for yield and spray-dried asparagus powders were investigated for their physicochemical characteristics. We were vary the range of the temperature, air flow rate, dextrin rate and set the best method for the functionality content of asparagus. Asparagus was spray - dried using 100 °C water extraction with high yield and high rutin content. The maximum spray drying yield was obtained at 15.55% of MD ratio, 167.87 °C of inlet temperature and 50.00 mL/min of air flow rate. There will be additional processed goods development made with what we have found. Funding Sources This study was supported by 2018 Regional Specialized Technology Development Project, Rural Development Administration, Republic of Korea. Supporting Tables, Images and/or Graphs

scholarly journals Microencapsulation of Copper(II) Sulfate in Ionically Cross-Linked Chitosan by Spray Drying for the Development of Irreversible Moisture Indicators in Paper Packaging

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2039 ◽  
Author(s):  
Sandra Rojas-Lema ◽  
Jorge Terol ◽  
Eduardo Fages ◽  
Rafael Balart ◽  
Luis Quiles-Carrillo ◽  
...  
Keyword(s):  
Spray Drying ◽  
Flow Rate ◽  
Gas Flow ◽  
Sodium Tripolyphosphate ◽  
Great Promise ◽  
Cross Linking ◽  
Inlet Temperature ◽  
Chitosan Microparticles ◽  
Cellulose Substrates

Copper(II) sulfate-loaded chitosan microparticles were herein prepared using ionic cross-linking with sodium tripolyphosphate (STPP) followed by spray drying. The microencapsulation process was optimal using an inlet temperature of 180 °C, a liquid flow-rate of 290 mL/h, an aspiration rate of 90%, and an atomizing gas flow-rate of 667 nL/h. Chitosan particles containing copper(II) sulfate of approximately 4 µm with a shrunken-type morphology were efficiently attained and, thereafter, fixated on a paper substrate either via cross-linking with STPP or using a chitosan hydrogel. The latter method led to the most promising system since it was performed at milder conditions and the original paper quality was preserved. The developed cellulose substrates were reduced and then exposed to different humidity conditions and characterized using colorimetric measurements in order to ascertain their potential as irreversible indicators for moisture detection. The results showed that the papers coated with the copper(II) sulfate-containing chitosan microparticles were successfully able to detect ambient moisture shown by the color changes of the coatings from dark brown to blue, which can be easily seen with the naked eye. Furthermore, the chitosan microparticles yielded no cytotoxicity in an in vitro cell culture experiment. Therefore, the cellulose substrates herein developed hold great promise in paper packaging as on-package colorimetric indicators for monitoring moisture in real time.

scholarly journals Process Optimization and Upscaling of Spray-Dried Drug-Amino acid Co-Amorphous Formulations

Pharmaceutics ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 24 ◽  
Author(s):  
Georgia Kasten ◽  
Íris Duarte ◽  
Maria Paisana ◽  
Korbinian Löbmann ◽  
Thomas Rades ◽  
...  
Keyword(s):  
Particle Size ◽  
Bulk Density ◽  
Spray Drying ◽  
Flow Rate ◽  
Gas Flow ◽  
Pilot Scale ◽  
High Yield ◽  
Outlet Temperature ◽  
Gas Flow Rate ◽  
Drying Chamber

The feasibility of upscaling the formulation of co-amorphous indomethacin-lysine from lab-scale to pilot-scale spray drying was investigated. A 22 full factorial design of experiments (DoE) was employed at lab scale. The atomization gas flow rate (Fatom, from 0.5 to 1.4 kg/h) and outlet temperature (Tout, from 55 to 75 °C) were chosen as the critical process parameters. The obtained amorphization, glass transition temperature, bulk density, yield, and particle size distribution were chosen as the critical quality attributes. In general, the model showed low Fatom and high Tout to be beneficial for the desired product characteristics (a co-amorphous formulation with a low bulk density, high yield, and small particle size). In addition, only a low Fatom and high Tout led to the desired complete co-amorphization, while a minor residual crystallinity was observed with the other combinations of Fatom and Tout. Finally, upscaling to a pilot scale spray dryer was carried out based on the DoE results; however, the drying gas flow rate and the feed flow rate were adjusted to account for the different drying chamber geometries. An increased likelihood to achieve complete amorphization, because of the extended drying chamber, and hence an increased residence time of the droplets in the drying gas, was found in the pilot scale, confirming the feasibility of upscaling spray drying as a production technique for co-amorphous systems.

scholarly journals CFD Simulation of Hydrogen Generation and Methane Combustion Inside a Water Splitting Membrane Reactor

Energies ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 7175
Author(s):  
Te Zhao ◽  
Chusheng Chen ◽  
Hong Ye
Keyword(s):  
Water Splitting ◽  
Flow Rate ◽  
Membrane Reactor ◽  
Gas Flow ◽  
Cfd Simulation ◽  
Hydrogen Generation ◽  
Methane Combustion ◽  
Inlet Temperature ◽  
Hydrogen Yield ◽  
Gas Flow Rate

Hydrogen production from water splitting remains difficult due to the low equilibrium constant (e.g., Kp ≈ 2 × 10−8 at 900 °C). The coupling of methane combustion with water splitting in an oxygen transport membrane reactor can shift the water splitting equilibrium toward dissociation by instantaneously removing O2 from the product, enabling the continuous process of water splitting and continuous generation of hydrogen, and the heat required for water splitting can be largely compensated for by methane combustion. In this work, a CFD simulation model for the coupled membrane reactor was developed and validated. The effects of the sweep gas flow rate, methane content and inlet temperature on the reactor performance were investigated. It was found that coupling of methane combustion with water splitting could significantly improve the hydrogen generation capacity of the membrane reactor. Under certain conditions, the average hydrogen yield with methane combustion could increase threefold compared to methods that used no coupling of combustion. The methane conversion decreases while the hydrogen yield increases with the increase in sweep gas flow rate or methane content. Excessive methane is required to ensure the hydrogen yield of the reactor. Increasing the inlet temperature can increase the membrane temperature, methane conversion, oxygen permeation rate and hydrogen yield.

scholarly journals Numerical Investigation on the Evaporation Performance of Desulfurization Wastewater in a Spray Drying Tower without Deflectors

Coatings ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1022
Author(s):  
Debo Li ◽  
Ning Zhao ◽  
Yongxin Feng ◽  
Zhiwen Xie
Keyword(s):  
Spray Drying ◽  
Droplet Size ◽  
Flow Rate ◽  
Flue Gas ◽  
Gas Flow ◽  
Initial Temperature ◽  
Gas Temperature ◽  
Gas Flow Rate ◽  
Complete Evaporation ◽  
Desulfurization Wastewater

The desulfurization wastewater evaporation technology with flue gas has been widely applied to dispose of desulfurization wastewater. This paper investigates the effect of flue gas flow rate and temperature, wastewater flow rate and initial temperature, and droplet size on the evaporation performance of the desulfurization wastewater in a spray drying tower without deflectors. The results show that the flue gas flow rate and temperature affect the evaporation performance of desulfurization wastewater. The larger flow rate and higher temperature of flue gas correspond to the faster evaporation speed and the shorter complete evaporation distance of the wastewater droplet. Decreasing the flow rate and increasing the initial temperature of the desulfurization wastewater is advantageous to enhance the evaporation speed and shorten the complete evaporation distance of the wastewater droplet. Reducing the droplet size is beneficial to improve the evaporation performance of the desulfurization wastewater. The orthogonal test results show that the factors affecting droplet evaporation performance are ranked as follows: flue gas flow rate > wastewater flow rate > flue gas temperature > wastewater initial temperature > droplet size. Considering the evaporation ratio and the complete evaporation distance, the optimal setting is 14.470 kg/s for flue gas flow rate, 385 °C for flue gas temperature, 0.582 kg/s for wastewater flow rate, 25 °C for wastewater initial temperature, and 60 μm for droplet size. These studied results can provide valuable information to improve the operational performance of the desulfurization wastewater evaporation technology with flue gas.

SCIENTIFIC AND ENGINEERING PRINCIPLES OF EFFICIENT FUEL USE AND ENVIRONMENTALLY FRIENDLY GAS COMBUSTION IN STOVE PLATES. PART 1. MODERN STATE-OF-THE-ART AND DIRECTIONS FOR IMPROVEMENT THE GAS BURNING IN DOMESTIC GAS COOKERS

2017 ◽  
pp. 3-18 ◽  
Author(s):  
B.S. Soroka ◽  
V.V. Horupa
Keyword(s):  
Natural Gas ◽  
Flow Rate ◽  
Gas Flow ◽  
Renewable Energy Sources ◽  
Combustion Process ◽  
Orifice Diameter ◽  
Firing Process ◽  
Gas Flow Rate ◽  
Gas Combustion ◽  
Gas Stoves

Natural gas NG consumption in industry and energy of Ukraine, in recent years falls down as a result of the crisis in the country’s economy, to a certain extent due to the introduction of renewable energy sources along with alternative technologies, while in the utility sector the consumption of fuel gas flow rate enhancing because of an increase the number of consumers. The natural gas is mostly using by domestic purpose for heating of premises and for cooking. These items of the gas utilization in Ukraine are already exceeding the NG consumption in industry. Cooking is proceeding directly in the living quarters, those usually do not meet the requirements of the Ukrainian norms DBN for the ventilation procedures. NG use in household gas stoves is of great importance from the standpoint of controlling the emissions of harmful components of combustion products along with maintenance the satisfactory energy efficiency characteristics of NG using. The main environment pollutants when burning the natural gas in gas stoves are including the nitrogen oxides NOx (to a greater extent — highly toxic NO2 component), carbon oxide CO, formaldehyde CH2O as well as hydrocarbons (unburned UHC and polyaromatic PAH). An overview of environmental documents to control CO and NOx emissions in comparison with the proper norms by USA, EU, Russian Federation, Australia and China, has been completed. The modern designs of the burners for gas stoves are considered along with defining the main characteristics: heat power, the natural gas flow rate, diameter of gas orifice, diameter and spacing the firing openings and other parameters. The modern physical and chemical principles of gas combustion by means of atmospheric ejection burners of gas cookers have been analyzed from the standpoints of combustion process stabilization and of ensuring the stability of flares. Among the factors of the firing process destabilization within the framework of analysis above mentioned, the following forms of unstable combustion/flame unstabilities have been considered: flashback, blow out or flame lifting, and the appearance of flame yellow tips. Bibl. 37, Fig. 11, Tab. 7.

On the Influence of Fluctuating Flow Rate Upon the Performance and Behaviour of Isothermal Reacting Systems

1998 ◽  
Vol 63 (6) ◽  
pp. 881-898
Author(s):  
Otakar Trnka ◽  
Miloslav Hartman
Keyword(s):  
Flow Rate ◽  
Gas Flow ◽  
Fluidized Bed Reactor ◽  
Computational Techniques ◽  
Continuous Stirred Tank Reactor ◽  
Gas Flow Rate ◽  
Solid Reaction ◽  
Continuous Stirred Tank ◽  
And Performance ◽  
Reacting Systems

Three simple computational techniques are proposed and employed to demonstrate the effect of fluctuating flow rate of feed on the behaviour and performance of an isothermal, continuous stirred tank reactor (CSTR). A fluidized bed reactor (FBR), in which a non-catalytic gas-solid reaction occurs, is also considered. The influence of amplitude and frequency of gas flow rate fluctuations on reactant concentrations at the exit of the CSTR is shown in four different situations.

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