scholarly journals Characterization of Novel Rhabdoviruses in Chinese Bats

Viruses ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 64
Author(s):  
Dong-Sheng Luo ◽  
Bei Li ◽  
Xu-Rui Shen ◽  
Ren-Di Jiang ◽  
Yan Zhu ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Retrospective Study ◽  
Rna Viruses ◽  
Ecological Niches ◽  
The Novel ◽  
Chain Reaction ◽  
Bat Virus ◽  
Complete Genomes ◽  
Polymerase Chain

Bats, the second largest order of mammals worldwide, harbor specific characteristics such as sustaining flight, a special immune system, unique habits, and ecological niches. In addition, they are the natural reservoirs of a variety of emerging or re-emerging zoonotic pathogens. Rhabdoviridae is one of the most diverse families of RNA viruses, which consists of 20 ecologically diverse genera, infecting plants, mammals, birds, reptiles, and fish. To date, three bat-related genera are described, named Lyssavirus, Vesiculovirus, and Ledantevirus. However, the prevalence and the distribution of these bat-related rhabdoviruses remain largely unknown, especially in China. To fill this gap, we performed a large molecular retrospective study based on the real-time reverse transcription polymerase chain reaction (RT-qPCR) detection of lyssavirus in bat samples (1044 brain and 3532 saliva samples, from 63 different bat species) originating from 21 provinces of China during 2006–2018. None of them were positive for lyssavirus, but six bat brains (0.6%) of Rhinolophus bat species, originating from Hubei and Hainan provinces, were positive for vesiculoviruses or ledanteviruses. Based on complete genomes, these viruses were phylogenetically classified into three putative new species, tentatively named Yinshui bat virus (YSBV), Taiyi bat virus (TYBV), and Qiongzhong bat virus (QZBV). These results indicate the novel rhabdoviruses circulated in different Chinese bat populations.

Characterization of the novel HLA‐A*31:01:34 allele by polymerase chain reaction sequencing‐based typing

HLA ◽  
2020 ◽  
Vol 96 (4) ◽  
pp. 502-504 ◽  
Author(s):  
Li Wang ◽  
Fang Wang ◽  
Wei Zhang ◽  
Ji He ◽  
Faming Zhu
Keyword(s):  
Polymerase Chain Reaction ◽  
The Novel ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Reaction Sequencing

scholarly journals Detection and characterization of human pathogenic viruses circulating in community wastewater using multi target microarrays and polymerase chain reaction

2013 ◽  
Vol 11 (4) ◽  
pp. 659-670 ◽  
Author(s):  
Mark V. M. Wong ◽  
Syed A. Hashsham ◽  
Erdogan Gulari ◽  
Jean-Marie Rouillard ◽  
Tiong Gim Aw ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Transmission ◽  
Rna Viruses ◽  
Enteric Viruses ◽  
Sewage Pollution ◽  
Chain Reaction ◽  
Viral Pathogens ◽  
Pathogenic Viruses ◽  
Polymerase Chain

Sewage pollution remains the most significant source of human waterborne pathogens. This study describes the detection and characterization of human enteric viruses in community wastewaters using cell culture coupled with multiple target microarrays (with a total of 780 unique probes targeting 27 different groups of both DNA and RNA viruses) and polymerase chain reaction (PCR) assays. Over a 13-month sampling period, RNA viruses (astroviruses and enteroviruses) were more frequently detected compared to DNA viruses (adenoviruses, particularly type 41 and BK polyomavirus). Overall, many more viruses were shed during the winter months (December–February) compared to the summer months. Exploration of the multiple types of enteric viruses particularly in winter months identified much more significant prevalence of key viral pathogens associated with sewage pollution of the water environment than previously realized and seasonal disinfection used in some parts of the world may lead to a seeding of ambient waters. Molecular characterization of pathogenic viruses in community wastewater will improve the understanding of the potential risk of waterborne disease transmission of viral pathogens.

Characterization of the novel HLA‐C*01:154 allele by polymerase chain reaction sequencing‐based typing

HLA ◽  
2020 ◽  
Vol 95 (5) ◽  
pp. 498-499 ◽  
Author(s):  
Xuan You ◽  
Wei Wang ◽  
Wei Zhang ◽  
Ji He ◽  
Faming Zhu
Keyword(s):  
Polymerase Chain Reaction ◽  
The Novel ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Reaction Sequencing

Characterization of Giardia duodenalis by polymerase-chain-reaction fingerprinting

1998 ◽  
Vol 84 (9) ◽  
pp. 707-714 ◽  
Author(s):  
Wieger L. Homan ◽  
Margriet Gilsing ◽  
Hafida Bentala ◽  
Louis Limper ◽  
Frans van Knapen
Keyword(s):  
Polymerase Chain Reaction ◽  
Giardia Duodenalis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Rhodococcus equi Infections in Goats: Characterization of Virulence Plasmids

2017 ◽  
Vol 55 (2) ◽  
pp. 273-276 ◽  
Author(s):  
Lauren W. Stranahan ◽  
Quinci D. Plumlee ◽  
Sara D. Lawhon ◽  
Noah D. Cohen ◽  
Laura K. Bryan
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Nodes ◽  
Rhodococcus Equi ◽  
Caseous Lymphadenitis ◽  
Chain Reaction ◽  
Disseminated Infection ◽  
Virulence Plasmids ◽  
Visceral Organs ◽  
Polymerase Chain

Rhodococcus equi is an uncommon cause of systemic pyogranulomatous infections in goats with macroscopic similarities to caseous lymphadenitis caused by Corynebacterium pseudotuberculosis. Caprine cases have previously been reported to be caused by avirulent R. equi strains. Six cases of R. equi infection in goats yielding 8 R. equi isolates were identified from 2000 to 2017. Lesions varied from bronchopneumonia, vertebral and humeral osteomyelitis, and subcutaneous abscesses, to disseminated infection involving the lungs, lymph nodes, and multiple visceral organs. Isolates of R. equi from infected goats were analyzed by polymerase chain reaction for R. equi virulence-associated plasmid ( vap) genes. Seven of 8 isolates carried the VapN plasmid, originally characterized in bovine isolates, while 1 isolate lacked virulence plasmids and was classified as avirulent. The VapN plasmid has not been described in isolates cultured from goats.

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