scholarly journals Viral Genomic Characterization and Replication Pattern of Human Polyomaviruses in Kidney Transplant Recipients

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1280
Author(s):  
Lucia Signorini ◽  
Maria Dolci ◽  
Evaldo Favi ◽  
Caterina Colico ◽  
Mariano Ferraresso ◽  
...  
Keyword(s):  
Kidney Transplant ◽  
Viral Reactivation ◽  
Human Polyomavirus ◽  
Replication Pattern ◽  
Kidney Transplant Recipients ◽  
Genotype I ◽  
Bk Polyomavirus ◽  
Genomic Characterization ◽  
Sequences Analysis

Human Polyomavirus (HPyV) infections are common, ranging from 60% to 100%. In kidney transplant (KTx) recipients, HPyVs have been associated with allograft nephropathy, progressive multifocal leukoencephalopathy, and skin cancer. Whether such complications are caused by viral reactivation or primary infection transmitted by the donor remains debated. This study aimed to investigate the replication pattern and genomic characterization of BK Polyomavirus (BKPyV), JC Polyomavirus (JCPyV), and Merkel Cell Polyomavirus (MCPyV) infections in KTx. Urine samples from 57 KTx donor/recipient pairs were collected immediately before organ retrieval/transplant and periodically up to post-operative day 540. Specimens were tested for the presence of BKPyV, JCPyV, and MCPyV genome by virus-specific Real-Time PCR and molecularly characterized. HPyVs genome was detected in 49.1% of donors and 77.2% of recipients. Sequences analysis revealed the archetypal strain for JCPyV, TU and Dunlop strains for BKPyV, and IIa-2 strain for MCPyV. VP1 genotyping showed a high frequency for JCPyV genotype 1 and BKPyV genotype I. Our experience demonstrates that after KTx, HPyVs genome remains stable over time with no emergence of quasi-species. HPyVs strains isolated in donor/recipient pairs are mostly identical, suggesting that viruses detected in the recipient may be transmitted by the allograft.

scholarly journals BK Polyomavirus Micro-RNAs: Time Course and Clinical Relevance in Kidney Transplant Recipients

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 351
Author(s):  
Baptiste Demey ◽  
Véronique Descamps ◽  
Claire Presne ◽  
Francois Helle ◽  
Catherine Francois ◽  
...  
Keyword(s):  
Viral Replication ◽  
Kidney Transplant ◽  
Clinical Relevance ◽  
Graft Loss ◽  
First Year ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Post Transplantation ◽  
Bk Polyomavirus ◽  
Micro Rnas

Background: Kidney transplant recipients (KTRs) are exposed to a high risk of BK polyomavirus (BKPyV) replication, which in turn may lead to graft loss. Although the microRNAs (miRNAs) bkv-miR-B1-3p and bkv-miR-B1-5p are produced during the viral cycle, their putative value as markers of viral replication has yet to be established. In KTRs, the clinical relevance of the changes over time in BKPyV miRNA levels has not been determined. Methods: In a retrospective study, we analyzed 186 urine samples and 120 plasma samples collected from 67 KTRs during the first year post-transplantation. Using a reproducible, standardized, quantitative RT-PCR assay, we measured the levels of bkv-miR-B1-3p and bkv-miR-B1-5p (relative to the BKPyV DNA load). Results: Detection of the two miRNAs had low diagnostic value for identifying patients with DNAemia or for predicting DNAuria during follow-up. Seven of the 14 KTRs with a sustained BKPyV infection within the first year post-transplantation showed a progressive reduction in the DNA load and then a rapid disappearance of the miRNAs. DNA and miRNA loads were stable in the other seven KTRs. Conclusions: After the DNA-based diagnosis of BKPyV infection in KTRs, bkv-miR-B1-3p and bkv-miR-B1-5p levels in the urine might be valuable markers for viral replication monitoring and thus might help physicians to avoid an excessive reduction in the immunosuppressive regimen.

scholarly journals Introduction of the Grayscale Median for Ultrasound Tissue Characterization of the Transplanted Kidney

Diagnostics ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 390
Author(s):  
Camilo G. Sotomayor ◽  
Stan Benjamens ◽  
Hildebrand Dijkstra ◽  
Derya Yakar ◽  
Cyril Moers ◽  
...  
Keyword(s):  
Kidney Transplant ◽  
Tissue Characterization ◽  
Kidney Transplant Recipients ◽  
Systematic Assessment ◽  
Ultrasound Tissue Characterization ◽  
Adult Kidney ◽  
Mersenne Twister ◽  
History Of

Ultrasound examination is advised for early post-kidney transplant assessment. Grayscale median (GSM) quantification is novel in the kidney transplant field, with no systematic assessment previously reported. In this prospective cohort study, we measured the post-operative GSM in a large cohort of adult kidney transplant recipients (KTR) who consecutively underwent Doppler ultrasound directly after transplantation (within 24 h), compared it with GSM in nontransplanted patients, and investigated its association with baseline and follow-up characteristics. B-mode images were used to calculate the GSM in KTR and compared with GSM data in nontransplanted patients, as simulated from summary statistics of the literature using a Mersenne twister algorithm. The association of GSM with baseline and 1-year follow-up characteristics were studied by means of linear regression analyses. In 282 KTR (54 ± 15 years old, 60% male), the median (IQR) GSM was 55 (45–69), ranging from 22 to 124 (coefficient of variation = 7.4%), without differences by type of donation (p = 0.28). GSM in KTR was significantly higher than in nontransplanted patients (p < 0.001), and associated with systolic blood pressure, history of cardiovascular disease, and donor age (std. β = 0.12, −0.20, and 0.13, respectively; p < 0.05 for all). Higher early post-kidney transplant GSM was not associated with 1-year post-kidney transplant function parameters (e.g., measured and estimated glomerular filtration rate). The data provided in this study could be used as first step for further research on the application of early postoperative ultrasound in KTR.

BK polyomavirus viremia and antibody responses of pediatric kidney transplant recipients in Finland

2018 ◽  
Vol 23 (1) ◽  
pp. e13324 ◽  
Author(s):  
Jenni Miettinen ◽  
Irmeli Lautenschlager ◽  
Fabian Weissbach ◽  
Marion Wernli ◽  
Eeva Auvinen ◽  
...  
Keyword(s):  
Kidney Transplant ◽  
Antibody Responses ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Bk Polyomavirus

Clinical utility of leflunomide for BK polyomavirus associated nephropathy in kidney transplant recipients: A multicenter retrospective study

2019 ◽  
Vol 21 (2) ◽  
pp. e13058 ◽  
Author(s):  
Nicolas Keller ◽  
Simon Duquennoy ◽  
Anne Conrad ◽  
Samira Fafi‐Kremer ◽  
Emmanuel Morelon ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Kidney Transplant ◽  
Clinical Utility ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Bk Polyomavirus

Role of the Early Association of a mTOR Inhibitor and Tacrolimus in BK Polyomavirus Infection in Kidney Transplant Recipients

2018 ◽  
Vol 102 ◽  
pp. S566
Author(s):  
Veronica Lopéz ◽  
Teresa Vazquez ◽  
Cristina Jironda ◽  
Mercedes Cabello ◽  
Juana Alonso ◽  
...  
Keyword(s):  
Kidney Transplant ◽  
Mtor Inhibitor ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Bk Polyomavirus

CHARACTERIZATION OF B CELL ANTIBODIES IN KIDNEY TRANSPLANT RECIPIENTS

1983 ◽  
Vol 35 (5) ◽  
pp. 446-451 ◽  
Author(s):  
Gabriel Nunez ◽  
John J. McPhaul ◽  
Peter Stastny
Keyword(s):  
B Cell ◽  
Kidney Transplant ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients

scholarly journals Pre-transplant immune factors may be associated with BK polyomavirus reactivation in kidney transplant recipients

PLoS ONE ◽  
2017 ◽  
Vol 12 (5) ◽  
pp. e0177339 ◽  
Author(s):  
David DeWolfe ◽  
Jinal Gandhi ◽  
Matthew R. Mackenzie ◽  
Thomas A. Broge ◽  
Evelyn Bord ◽  
...  
Keyword(s):  
Kidney Transplant ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Bk Polyomavirus ◽  
Immune Factors
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