scholarly journals The Diversity and Distribution of Viruses Associated with Culex annulirostris Mosquitoes from the Kimberley Region of Western Australia

Viruses ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 717 ◽  
Author(s):  
Simon H. Williams ◽  
Avram Levy ◽  
Rachel A. Yates ◽  
Nilusha Somaweera ◽  
Peter J. Neville ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
High Throughput ◽  
Western Australia ◽  
High Throughput Sequencing ◽  
Marked Variation ◽  
Chain Reaction ◽  
Virus Prevalence ◽  
Culex Annulirostris ◽  
Polymerase Chain ◽  
Viral Sequences

Metagenomics revealed an impressive breadth of previously unrecognized viruses. Here, we report the virome of the Culex annulirostris Skuse mosquito, an important vector of pathogenic arboviruses in Australia. Mosquitoes were collected from three sites in the Kimberley region of Western Australia. Unbiased high-throughput sequencing (HTS) revealed the presence of 16 novel viral sequences that share less than 90% identity with known viruses. None were closely related to pathogenic arboviruses. Viruses were distributed unevenly across sites, indicating a heterogeneous Cx. annulirostris virome. Polymerase chain reaction assays confirmed HTS data and identified marked variation between the virus prevalence identified at each site.

scholarly journals Sustained high-throughput polymerase chain reaction diagnostics during the European epidemic of Bluetongue virus serotype 8

2012 ◽  
Vol 24 (3) ◽  
pp. 469-478 ◽  
Author(s):  
Piet A. van Rijn ◽  
René G. Heutink ◽  
Jan Boonstra ◽  
Hans A. Kramps ◽  
René G. P. van Gennip
Keyword(s):  
Polymerase Chain Reaction ◽  
High Throughput ◽  
Bluetongue Virus ◽  
Chain Reaction ◽  
Virus Serotype ◽  
Polymerase Chain

Detection of Genomic Sequences of Respiratory Syncytial Virus in Otitis Media with Effusion in Children

1992 ◽  
Vol 101 (10_suppl) ◽  
pp. 7-10 ◽  
Author(s):  
Yoshitaka Okamoto ◽  
Kazuo Kudo ◽  
Koji Shirotori ◽  
Misao Nakazawa ◽  
Eiko Ito ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Respiratory Syncytial Virus ◽  
Otitis Media ◽  
Otitis Media With Effusion ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Rsv Infection ◽  
Polymerase Chain ◽  
Syncytial Virus ◽  
Viral Sequences

The reverse transcriptase—polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.

scholarly journals Discovery of Cocirculating Ross River Virus and Barmah Forest Virus At Wide Bay Military Training Area, Northeastern Australia

2019 ◽  
Vol 35 (3) ◽  
pp. 220-223
Author(s):  
Jo Kizu ◽  
Christina Neuman ◽  
Luke Le Grand ◽  
Wenjun Liu
Keyword(s):  
Polymerase Chain Reaction ◽  
Phylogenetic Analysis ◽  
Military Training ◽  
Ross River Virus ◽  
Chain Reaction ◽  
Culex Annulirostris ◽  
Australian Defence Force ◽  
Polymerase Chain ◽  
Human Infections ◽  
Training Area

ABSTRACT An arbovirus surveillance military exercise was conducted to assess the risk of Ross River virus (RRV) and Barmah Forest virus (BFV) in the Australian Defence Force (ADF) Wide Bay training area (WBTA), northeastern Australia, in April 2018. Of the 5,540 female mosquitoes collected, 3,702 were screened for RRV and BFV by quantitative reverse transcription–polymerase chain reaction in a field laboratory. One pool of Verrallina funerea was positive for RRV and 8 pools (7 pools of Aedes vigilax and 1 pool of Culex annulirostris) were positive for BFV. Phylogenetic analysis of the complete nucleotide sequence of the E2 protein subgrouped both RRV and BFV with viruses previously isolated from human infections, indicating the potential risk of RRV and BFV infection to ADF personnel while training in WBTA. This is the 1st time that both RRV and BFV have been detected in a military training area.

Quantum dots for a high-throughput Pfu polymerase based multi-round polymerase chain reaction (PCR)

The Analyst ◽  
2018 ◽  
Vol 143 (5) ◽  
pp. 1259-1267 ◽  
Author(s):  
Fuming Sang ◽  
Zhizhou Zhang ◽  
Lin Yuan ◽  
Deli Liu
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantum Dots ◽  
High Throughput ◽  
Chain Reaction ◽  
Round Polymerase Chain Reaction ◽  
Polymerase Chain

We developed a Pfu polymerase based multi-round PCR technique assisted by quantum dots (QDs).

scholarly journals Updating the Quarantine Status of Prunus Infecting Viruses in Australia

Viruses ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 246 ◽  
Author(s):  
Wycliff M. Kinoti ◽  
Narelle Nancarrow ◽  
Alison Dann ◽  
Brendan C. Rodoni ◽  
Fiona E. Constable
Keyword(s):  
Polymerase Chain Reaction ◽  
High Throughput Sequencing ◽  
Mottle Virus ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Virus Family ◽  
First Report ◽  
Hop Stunt Viroid ◽  
Polymerase Chain ◽  
Stem Pitting

One hundred Prunus trees, including almond (P. dulcis), apricot (P. armeniaca), nectarine (P. persica var. nucipersica), peach (P. persica), plum (P. domestica), purple leaf plum (P. cerasifera) and sweet cherry (P. avium), were selected from growing regions Australia-wide and tested for the presence of 34 viruses and three viroids using species-specific reverse transcription-polymerase chain reaction (RT-PCR) or polymerase chain reaction (PCR) tests. In addition, the samples were tested using some virus family or genus-based RT-PCR tests. The following viruses were detected: Apple chlorotic leaf spot virus (ACLSV) (13/100), Apple mosaic virus (ApMV) (1/100), Cherry green ring mottle virus (CGRMV) (4/100), Cherry necrotic rusty mottle virus (CNRMV) (2/100), Cherry virus A (CVA) (14/100), Little cherry virus 2 (LChV2) (3/100), Plum bark necrosis stem pitting associated virus (PBNSPaV) (4/100), Prune dwarf virus (PDV) (3/100), Prunus necrotic ringspot virus (PNRSV) (52/100), Hop stunt viroid (HSVd) (9/100) and Peach latent mosaic viroid (PLMVd) (6/100). The results showed that PNRSV is widespread in Prunus trees in Australia. Metagenomic high-throughput sequencing (HTS) and bioinformatics analysis were used to characterise the genomes of some viruses that were detected by RT-PCR tests and Apricot latent virus (ApLV), Apricot vein clearing associated virus (AVCaV), Asian Prunus Virus 2 (APV2) and Nectarine stem pitting-associated virus (NSPaV) were also detected. This is the first report of ApLV, APV2, CGRMV, CNRNV, LChV1, LChV2, NSPaV and PBNSPaV occurring in Australia. It is also the first report of ASGV infecting Prunus species in Australia, although it is known to infect other plant species including pome fruit and citrus.

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