Quantum dots for a high-throughput Pfu polymerase based multi-round polymerase chain reaction (PCR)

The Analyst ◽  
2018 ◽  
Vol 143 (5) ◽  
pp. 1259-1267 ◽  
Author(s):  
Fuming Sang ◽  
Zhizhou Zhang ◽  
Lin Yuan ◽  
Deli Liu
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantum Dots ◽  
High Throughput ◽  
Chain Reaction ◽  
Round Polymerase Chain Reaction ◽  
Polymerase Chain

We developed a Pfu polymerase based multi-round PCR technique assisted by quantum dots (QDs).

scholarly journals Sustained high-throughput polymerase chain reaction diagnostics during the European epidemic of Bluetongue virus serotype 8

2012 ◽  
Vol 24 (3) ◽  
pp. 469-478 ◽  
Author(s):  
Piet A. van Rijn ◽  
René G. Heutink ◽  
Jan Boonstra ◽  
Hans A. Kramps ◽  
René G. P. van Gennip
Keyword(s):  
Polymerase Chain Reaction ◽  
High Throughput ◽  
Bluetongue Virus ◽  
Chain Reaction ◽  
Virus Serotype ◽  
Polymerase Chain

scholarly journals Evaluation of a 384–Well Format for High-Throughput Real-Time Reverse Transcription Polymerase Chain Reaction for Avian Influenza Testing

2009 ◽  
Vol 21 (5) ◽  
pp. 679-683 ◽  
Author(s):  
Pamela J. Ferro ◽  
Jason Osterstock ◽  
Bo Norby ◽  
Geoffrey T. Fosgate ◽  
Blanca Lupiani
Keyword(s):  
Polymerase Chain Reaction ◽  
Avian Influenza ◽  
Real Time ◽  
High Throughput ◽  
Reverse Transcription ◽  
Virus Isolation ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Response Planning ◽  
Polymerase Chain

As concerns over the global spread of highly pathogenic avian influenza H5N1 have heightened, more countries are faced with increased surveillance efforts and incident response planning for handling a potential outbreak. The incorporation of molecular techniques in most diagnostic laboratories has enabled fast and efficient testing of many agents of concern, including avian influenza. However, the need for high-throughput testing remains. In this study, the use of a 384–well format for high-throughput real-time reverse transcription polymerase chain reaction (real-time RT-PCR) testing for avian influenza is described. The analytical sensitivity of a real-time RT-PCR assay for avian influenza virus matrix gene with the use of both 96– and 384–well assay formats and serial dilutions of transcribed control RNA were comparable, resulting in similar limits of detection. Of 28 hunter-collected cloacal swabs that were positive by virus isolation, 26 (92.9%) and 27 (96.4%) were positive in the 96– and 384–well assays, respectively; of the 340 hunter-collected swabs that were negative by virus isolation, 45 (13.2%) and 23 (6.8%) were positive in the 96– and 384–well assays, respectively. The data presented herein supports the utility of the 384–well format in the event of an avian influenza outbreak for high-throughput real-time RT-PCR testing.

scholarly journals Amplification of SPPS150 andSalmonella typhiDNA with a high throughput oscillating flow polymerase chain reaction device

2010 ◽  
Vol 4 (2) ◽  
pp. 024103 ◽  
Author(s):  
D. Sugumar ◽  
Asma Ismail ◽  
Manickam Ravichandran ◽  
Ismail Aziah ◽  
L. X. Kong
Keyword(s):  
Polymerase Chain Reaction ◽  
High Throughput ◽  
Oscillating Flow ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Reaction Device
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