scholarly journals Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola

Viruses ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 231 ◽  
Author(s):  
Jia Zhou ◽  
Yuhua Wang ◽  
Xiaofei Liang ◽  
Changping Xie ◽  
Wenbo Liu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Rna Polymerase ◽  
Molecular Characterization ◽  
Open Reading Frame ◽  
Single Open Reading Frame ◽  
Rna Dependent Rna Polymerase ◽  
Reading Frame ◽  
The Family

Here, we report a novel (+) ssRNA mycovirus, Phoma matteucciicola ourmia-like virus 1 (PmOLV1), isolated from Phoma matteucciicola strain LG915-1. The genome of PmOLV1 was 2603 nucleotides long and contained a single open reading frame (ORF), which could be translated into a product of RNA-dependent RNA polymerase (RdRp) by both standard and mitochondrial genetic codons. Cellular fractionation assay indicated that PmOLV1 RNAs are likely more enriched in mitochondria than in cytoplasm. Phylogenetic analysis indicated that PmOLV1 is a new member of the genus Penoulivirus (recently proposed) within the family Botourmiaviridae.

scholarly journals Evidence for a Novel Partitivirus Isolated From Entomopathogenic Nematode Steinernema Ceratophorum

2021 ◽  
Author(s):  
Shuangchao Wang ◽  
Irfan Ahmed ◽  
Xianhui Li ◽  
Lihua Guo
Keyword(s):  
Phylogenetic Analysis ◽  
Entomopathogenic Nematode ◽  
Plasmopara Viticola ◽  
Open Reading Frame ◽  
Sequence Alignments ◽  
Rna Dependent Rna Polymerase ◽  
Multiple Sequence ◽  
Reading Frame ◽  
Multiple Sequence Alignments ◽  
The Family

Abstract Nematodes are abundant, yet little is known about their viruses. In this study, we report a novel partitivirus isolated from Entomopathogenic nematode specie “Steinernema ceratophorum, named as Steinernema ceratophorum Partiti-like Virus 1 (ScPV1). The complete genome of ScPV1 is comprised of two dsRNA segments, dsRNA1 (2352 bp) and dsRNA2 (2196 bp) in length. Both dsRNAs contained a single open reading frame (ORF), encoding a putative RNA-dependent RNA polymerase (RdRp) and a coat protein (CP), respectively. The sequences of the RdRp and CP showed the highest similarity (47% and 33% identity, respectively) to Plasmopara viticola associated Partitivirus 7. Multiple sequence alignments and phylogenetic analysis of RdRp of ScPV1 with other selected viruses indicated that ScPV1 is the new member of genus Betapartitivirus in the family Partitiviridae.

scholarly journals Complete Nucleotide Sequence of a Partitivirus from Rhizoctonia solani AG-1 IA Strain C24

Viruses ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 703 ◽  
Author(s):  
Chen Liu ◽  
Miaolin Zeng ◽  
Meiling Zhang ◽  
Canwei Shu ◽  
Erxun Zhou
Keyword(s):  
Phylogenetic Analysis ◽  
Amino Acid ◽  
Nucleotide Sequence ◽  
Rhizoctonia Solani ◽  
Sheath Blight ◽  
Open Reading Frame ◽  
Rice Sheath Blight ◽  
Rna Dependent Rna Polymerase ◽  
Reading Frame ◽  
The Family

The complete genome of a novel double-stranded (ds) RNA mycovirus, named as Rhizoctonia solani partitivirus 5 (RsPV5), isolated from rice sheath blight fungus R. solani AG-1 IA strain C24, was sequenced and analysed. RsPV5 consists of two segments, dsRNA-1 (1899 nucleotides) and dsRNA-2 (1787 nucleotides). DsRNA-1 has an open reading frame (ORF) 1 that potentially codes for a protein of 584 amino acid (aa) containing the conserved motifs of a RNA-dependent RNA polymerase (RdRp), and dsRNA-2 also contains a ORF 2, encoding a putative capsid protein (CP) of 513 aa. Phylogenetic analysis revealed that RsPV5 clustered together with six other viruses in an independent clade of the genus Alphapartitivirus, indicating that RsPV5 was a new member of the genus Alphapartitivirus, within the family Partitiviridae.

scholarly journals Molecular characterization of infectious bronchitis virus based on RNA‐dependent RNA polymerase gene

2020 ◽  
Vol 64 (8) ◽  
pp. 556-562
Author(s):  
Mozafar Hajijafari Anaraki ◽  
Nariman Sheikhi ◽  
Hadi Haghbin Nazarpak ◽  
Gholamreza Nikbakht Brujeni
Keyword(s):  
Rna Polymerase ◽  
Molecular Characterization ◽  
Infectious Bronchitis Virus ◽  
Polymerase Gene ◽  
Rna Dependent Rna Polymerase ◽  
Infectious Bronchitis ◽  
Rna Polymerase Gene

scholarly journals Molecular Characterization of Theileria orientalis Piroplasm Protein Encoded by an Open Reading Frame (To ORF2) in a Genomic Fragment

2004 ◽  
Vol 66 (8) ◽  
pp. 957-963 ◽  
Author(s):  
Jung-Yeon KIM ◽  
Naoaki YOKOYAMA ◽  
Sanjay KUMAR ◽  
Noboru INOUE ◽  
Kozo FUJISAKI ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Open Reading Frame ◽  
Genomic Fragment ◽  
Theileria Orientalis ◽  
Reading Frame

scholarly journals Cryphonectria hypovirus 3, a Virus Species in the Family Hypoviridae with a Single Open Reading Frame

Virology ◽  
1999 ◽  
Vol 265 (1) ◽  
pp. 66-73 ◽  
Author(s):  
C.D. Smart ◽  
W. Yuan ◽  
R. Foglia ◽  
D.L. Nuss ◽  
D.W. Fulbright ◽  
...  
Keyword(s):  
Open Reading Frame ◽  
Single Open Reading Frame ◽  
Virus Species ◽  
Reading Frame ◽  
The Family ◽  
Cryphonectria Hypovirus

scholarly journals Phylogeny and Characterization of Three nifH-Homologous Genes from Paenibacillus azotofixans

2003 ◽  
Vol 69 (6) ◽  
pp. 3658-3662 ◽  
Author(s):  
Quok-Cheong Choo ◽  
Mohd-Razip Samian ◽  
Nazalan Najimudin
Keyword(s):  
Phylogenetic Analysis ◽  
Methanogenic Archaea ◽  
Gene Organization ◽  
Open Reading Frame ◽  
The Other ◽  
Sequencing Analysis ◽  
Reading Frame ◽  
Homologous Genes ◽  
Dna Fragment

ABSTRACT In this paper, we report the cloning and characterization of three Paenibacillus azotofixans DNA regions containing genes involved in nitrogen fixation. Sequencing analysis revealed the presence of nifB1H1D1K1 gene organization in the 4,607-bp SacI DNA fragment. This is the first report of linkage of a nifB open reading frame upstream of the structural nif genes. The second (nifB2H2) and third (nifH3) nif homologues are confined within the 6,350-bp HindIII and 2,840-bp EcoRI DNA fragments, respectively. Phylogenetic analysis demonstrated that NifH1 and NifH2 form a monophyletic group among cyanobacterial NifH proteins. NifH3, on the other hand, clusters among NifH proteins of the highly divergent methanogenic archaea.

scholarly journals Characterization of Moraxella(Branhamella) catarrhalis lbpB, lbpA, and Lactoferrin Receptor orf3 Isogenic Mutants

1999 ◽  
Vol 67 (3) ◽  
pp. 1517-1520 ◽  
Author(s):  
Robert A. Bonnah ◽  
Henry Wong ◽  
Sheena M. Loosmore ◽  
Anthony B. Schryvers
Keyword(s):  
Moraxella Catarrhalis ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Orf3 Protein ◽  
Lactoferrin Receptor ◽  
The Family ◽  
Specific Receptors ◽  
Isogenic Mutants ◽  
Branhamella Catarrhalis

ABSTRACT Pathogenic members of the family Neisseriaceae produce specific receptors to acquire iron from their host’s lactoferrin and transferrin. Recently, putative Moraxella catarrhalislactoferrin receptor genes and a third open reading frame (lbpB, lbpA, and orf3) were cloned and sequenced. We describe the preliminary characterization of isogenic mutants deficient in LbpB, LbpA, or Orf3 protein.

Sign in / Sign up

Export Citation Format

Share Document