scholarly journals Novel Mammalian orthorubulavirus 5 Discovered as Accidental Cell Culture Contaminant

Viruses ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 777
Author(s):  
Brandi J. Feehan ◽  
Aleksey A. Penin ◽  
Alexey N. Mukhin ◽  
Deepak Kumar ◽  
Anna S. Moskvina ◽  
...  
Keyword(s):  
Cell Culture ◽  
Respiratory Symptoms ◽  
Cytopathic Effect ◽  
Divergent Strain ◽  
Lack Of Knowledge

A distinct Russian Mammalian orthorubulavirus 5 (PIV5) was detected in cell culture exhibiting cytopathic effect and hypothesized to be contaminated by a scientist with respiratory symptoms. The identification of the divergent strain indicated a lack of knowledge on the diversity of PIV5 strains and calls for surveillance of global PIV5 strains.

scholarly journals Classical Swine Fever Virus Leader Proteinase Npro Is Not Required for Viral Replication in Cell Culture

1998 ◽  
Vol 72 (9) ◽  
pp. 7681-7684 ◽  
Author(s):  
Jon-Duri Tratschin ◽  
Christian Moser ◽  
Nicolas Ruggli ◽  
Martin A. Hofmann
Keyword(s):  
Cell Culture ◽  
Classical Swine Fever Virus ◽  
Recombinant Virus ◽  
Cytopathic Effect ◽  
Classical Swine Fever ◽  
Growth Characteristics ◽  
Fever Virus ◽  
Subgenomic Rna ◽  
Viral Genes ◽  
Sequence Encoding

ABSTRACT The sequence encoding the viral leader proteinase Nprowas replaced by the murine ubiquitin gene in a full-length cDNA clone of the classical swine fever virus (CSFV) strain Alfort/187. The recombinant virus vA187-Ubi showed growth characteristics similar to those of the parent vA187-1 virus. At two occasions cells infected with vA187-Ubi exhibited a cytopathic effect and were found to contain a subgenomic viral RNA. This RNA lacked the same viral genes as the subgenomic RNA which has been found in all cytopathogenic CSFV strains analyzed so far, but it maintained the ubiquitin sequence.

Characteristics of a Mycoplasma hyorhinis strain contaminating a human fetal diploid cell line

1969 ◽  
Vol 15 (5) ◽  
pp. 451-454 ◽  
Author(s):  
J. Joncas ◽  
A. Chagnon ◽  
G. Lussier ◽  
V. Pavilanis
Keyword(s):  
Cell Culture ◽  
Kidney Cell ◽  
Cytopathic Effect ◽  
Fetal Lung ◽  
Monkey Kidney ◽  
Direct Immunofluorescence ◽  
Monkey Kidney Cell ◽  
Acridine Orange Staining ◽  
Kidney Cell Culture ◽  
Accessory Factor

A human fetal lung cell culture contaminant possessing most of the characteristics of that described by Girardi et al. (5) was shown to belong to the M. hyorhinis-GDL group of cytopathic mycoplasma by accessory factor neutralization test. The homotypic antiserum in this test neutralized the contaminant as well as three closely related strains while it could inhibit the growth of the contaminant only on agar. The mycoplasma could be detected in tissue cultures by the acridine orange staining technique and by direct immunofluorescence. These appeared as minute coccobacillary bodies either isolated or in clumps, predominantly extracellular in the original tissue culture but apparently intracellular in monkey kidney cell culture. A titer of 106 TCID50 per 0.1 ml was obtained in monkey kidney cell culture and the cytopathic effect induced in this culture was inhibited by pretreatment of the agent with 20% ether for 18 hours at 4 C, with tris buffer at pH 3 for 3 hours, or by heating at 56 C for 3 minutes. The cytopathic effect was suppressed by chlortetracycline (50 μg/ml) and neutralized with accessory factor by specific antiserum produced in rabbit up to a titer of 8192, whereas a variety of normal human and animal sera failed to do so. The source of the contaminant could not be determined.

Culicoides Oxystoma a Potential Vector for Transmission of Bluetongue Virus 16 in Southern India

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
Minakshi Prasad ◽  
Koushlesh Ranjan ◽  
Basanti Brar ◽  
Gaya Prasad
Keyword(s):  
Cell Culture ◽  
Nucleic Acid ◽  
Bluetongue Virus ◽  
Cytopathic Effect ◽  
Migration Pattern ◽  
Rt Pcr ◽  
Viral Nucleic Acid ◽  
Vp2 Gene ◽  
Potential Vector ◽  
Culicoides Oxystoma

Bluetongue (BT) is a Culicoides vector borne disease of domestic and wild ruminants. It is caused by bluetongue virus (BTV). BT is infectious but non-contagious disease. The head–thorax region of Culicoides Oxystoma vector trapped on animal farm in TN was triturated and inoculated in BHK- 21 cell culture. After few blind passages it showed cytopathic effect (CPE) in cell culture. After appearance of 75% cytopathic effect in BHK 21 cell culture, viral nucleic acid was extracted. The RNA-PAGE analysis showed BTV specific characteristics migration pattern of 3:3:3:1. Viral nucleic acid was allowed for cDNA synthesis followed by NS1 and VP2 gene based RT-PCR. The NS1 gene RT-PCR, CPE and specific migration pattern in RNA-PAGE confirmed the sample as BTV. The VP2 gene based serotype specific RT-PCR identified the isolate as BTV serotype 16. The study suggested that Culicoides oxystoma could be a potential vector for transmission of BTV in southern India.

Influence of inoculum size, incubation temperature, and cell culture density on virus detection in environmental samples

1985 ◽  
Vol 31 (11) ◽  
pp. 977-980 ◽  
Author(s):  
Pierre Payment ◽  
Michel Trudel
Keyword(s):  
Cell Culture ◽  
Cytopathic Effect ◽  
Incubation Temperature ◽  
Plaque Assay ◽  
Virus Titer ◽  
Inoculum Size ◽  
Most Probable Number ◽  
Probable Number ◽  
Culture Density ◽  
Coxsackievirus B

The influence of inoculum size and cell culture density on virus titer by cytopathic effect or plaque assay was studied using poliovirus type 1 and BGM (Buffalo green monkey) cells as a model for this evaluation. With a plaque assay system, a linear relationship was observed for an inoculum size of up 1 mL/25 cm2; a marked decrease in the number of plaques was observed when over 1 mL of sample was inoculated on this surface area. Cell culture density also affected virus titer; maximal titers were observed when cells were seeded at 25 000 to 75 000 cells/mL and incubated for 6 days before infection with the virus. Viral density, evaluated as most-probable-number and measured by cytopathic effect under liquid overlay, revealed that the viral titer was similar up to 1 mL inoculum and increased only when over 1 mL was inoculated. Cell density had no significant effect on the viral titer measured by the most-probable-number method and cytopathic effect. Inactivation of inoculum due to an incubation temperature of 37 °C for a short period was shown to be minimal for poliovirus type 1, reovirus type 2, coxsackievirus B-5, and the simian rotavirus SA-11. Longer inactivation time led to a 2 logs reduction of the infectious titer of coxsackievirus B-5 (in 48 h) while the other viruses showed a significant reduction in titer only after 96 h.

scholarly journals The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture

2002 ◽  
Vol 45 (2) ◽  
pp. 125-135 ◽  
Author(s):  
Beatriz S. Hasenack ◽  
Maria Valéria J. Botelho ◽  
Flávio Lauretti ◽  
Fernando L. de Melo ◽  
Janaina M. Orlandi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Cell Cultures ◽  
Concanavalin A ◽  
Gel Electrophoresis ◽  
Cytopathic Effect ◽  
Polyacrylamide Gel Electrophoresis ◽  
Plaque Assay ◽  
Virus Growth ◽  
Kinetics Of

Rotavirus (RV) strain SA-11 was studied with respect to its infectivity in MA-104 cell cultures and the effect of concanavalin A (ConA). Receptors for ConA at the surface of MA-104 cell were determined by fluorescence assay and specifically inhibited by D-mannose. The kinetics of virus growth was carried out by plaque assay. Electron microscopy and polyacrylamide gel electrophoresis were used for monitoring the experiments. It was concluded that RV replication was not affected consistently by ConA, however it interfered with the development of cytopathic effect (CPE) without altering virus yields.

scholarly journals Experimental evaluation of the activity of the product meflochine against coronavirus SАRS-CoV-2

2020 ◽  
Author(s):  
KN Filin ◽  
IA Berzin ◽  
VN Bykov ◽  
VD Gladkikh ◽  
SYa Loginova ◽  
...  
Keyword(s):  
Cell Culture ◽  
Antiviral Activity ◽  
Kidney Cell ◽  
Cytopathic Effect ◽  
Antiviral Effect ◽  
In Vitro Experiments ◽  
Monkey Kidney Cell ◽  
African Green Monkey Kidney ◽  
Green Monkey

When evaluating the effectiveness of the drug Mefloquine against SARS-Cov-2 coronavirus, in vitro experiments examined its toxicity for African green monkey kidney cell culture - Vero C1008, as well as antiviral activity against SARS-Cov-2, which was evaluated by suppressing the cytopathic effect the virus. A study of the toxicity of the drug Mefloquine showed that the concentration at which the drug exerts a cytopathic effect against 50% of Vero C1008 cells is 4.5 μg / ml. The maximum tolerated concentration (MTD) of Mefloquine is 2.25 μg / ml. A study of the effectiveness showed that 1 day after infection, the antiviral effect of Mefloquine was recorded when the drug was added 24 hours and 1 hour before infection with SARS-CoV-2, as well as when it was added 1 hour after infection, the cell culture was already at a concentration of 0.5 μg / ml Mefloquine at a concentration of 2 μg / ml, added to the Vero C1008 cell culture 1 hour after the introduction of SARS-CoV-2, completely blocked the action of the virus for 2 days after infection.

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