scholarly journals Cell Culture, Cytopathic Effect and Immunofluorescence Diagnosis of Viral Infection

2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Dilnessa T ◽  
Zeleke H
Keyword(s):  
Cell Culture ◽  
Viral Infection ◽  
Cytopathic Effect

scholarly journals Classical Swine Fever Virus Leader Proteinase Npro Is Not Required for Viral Replication in Cell Culture

1998 ◽  
Vol 72 (9) ◽  
pp. 7681-7684 ◽  
Author(s):  
Jon-Duri Tratschin ◽  
Christian Moser ◽  
Nicolas Ruggli ◽  
Martin A. Hofmann
Keyword(s):  
Cell Culture ◽  
Classical Swine Fever Virus ◽  
Recombinant Virus ◽  
Cytopathic Effect ◽  
Classical Swine Fever ◽  
Growth Characteristics ◽  
Fever Virus ◽  
Subgenomic Rna ◽  
Viral Genes ◽  
Sequence Encoding

ABSTRACT The sequence encoding the viral leader proteinase Nprowas replaced by the murine ubiquitin gene in a full-length cDNA clone of the classical swine fever virus (CSFV) strain Alfort/187. The recombinant virus vA187-Ubi showed growth characteristics similar to those of the parent vA187-1 virus. At two occasions cells infected with vA187-Ubi exhibited a cytopathic effect and were found to contain a subgenomic viral RNA. This RNA lacked the same viral genes as the subgenomic RNA which has been found in all cytopathogenic CSFV strains analyzed so far, but it maintained the ubiquitin sequence.

scholarly journals Identification and Semisynthesis of (–)-Anisomelic Acid as an Oral Antiviral Agent against SARS-CoV-2 in Mice

2021 ◽  
Author(s):  
Hai-Xin Yu ◽  
Nan Zheng ◽  
Chi-Tai Yeh ◽  
Chien-Ming Lee ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Oral Administration ◽  
Viral Infection ◽  
Lung Tissue ◽  
Cytopathic Effect ◽  
Antiviral Agent ◽  
Starting Material ◽  
Viral Titers

(–)-Anisomelic acid (AA), isolated from Anisomeles indica (L.) Kuntze (Labiatae) leaves, is a macrocyclic cembranolide with a trans-fused α-methylene-β-lactone motif. Cytopathic effect assays showed that the anti-SARS-CoV-2 effect of AA (IC50 = 4.3 μM) is comparable to that of remdesivir (IC50 = 2.1 μM), and more potent than that of molnupiravir (IC50 = 27.8 μM). Challenge studies in SARS-CoV-2-infected K18-hACE2 mice showed that oral administration of AA and remdesivir can both reduce the viral titers in the lung tissue at the same level. To facilitate drug discovery, we used a semisynthetic approach to shorten the project timelines. The enantioselective semisynthesis of AA from the naturally enriched and commercially available starting material (+)-costunolide was achieved in five steps with a 27% overall yield. The developed chemistry provides opportunities for developing AA-based novel ligands for selectively targeting proteins involved in viral infection.

Immunofluorescence and cell culture techniques in the diagnosis of viral infection of aborted bovine fetuses

1986 ◽  
Vol 118 (9) ◽  
pp. 242-243 ◽  
Author(s):  
M. Lucas ◽  
D. Westcott ◽  
S. Edwards ◽  
R. Newman ◽  
C. Swallow
Keyword(s):  
Cell Culture ◽  
Viral Infection ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Bovine Fetuses

scholarly journals Novel Mammalian orthorubulavirus 5 Discovered as Accidental Cell Culture Contaminant

Viruses ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 777
Author(s):  
Brandi J. Feehan ◽  
Aleksey A. Penin ◽  
Alexey N. Mukhin ◽  
Deepak Kumar ◽  
Anna S. Moskvina ◽  
...  
Keyword(s):  
Cell Culture ◽  
Respiratory Symptoms ◽  
Cytopathic Effect ◽  
Divergent Strain ◽  
Lack Of Knowledge

A distinct Russian Mammalian orthorubulavirus 5 (PIV5) was detected in cell culture exhibiting cytopathic effect and hypothesized to be contaminated by a scientist with respiratory symptoms. The identification of the divergent strain indicated a lack of knowledge on the diversity of PIV5 strains and calls for surveillance of global PIV5 strains.

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