scholarly journals New Insights into the Evolutionary and Genomic Landscape of Molluscum Contagiosum Virus (MCV) based on Nine MCV1 and Six MCV2 Complete Genome Sequences

Viruses ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 586 ◽  
Author(s):  
Tomaž Zorec ◽  
Denis Kutnjak ◽  
Lea Hošnjak ◽  
Blanka Kušar ◽  
Katarina Trčko ◽  
...  
Keyword(s):  
Evolutionary History ◽  
Large Scale ◽  
Nk Cell ◽  
Molluscum Contagiosum ◽  
Molluscum Contagiosum Virus ◽  
Genome Sequences ◽  
Viral Interference ◽  
Genomic Landscape ◽  
Genetic Landscape ◽  
Common Skin

Molluscum contagiosum virus (MCV) is the sole member of the Molluscipoxvirus genus and the causative agent of molluscum contagiosum (MC), a common skin disease. Although it is an important and frequent human pathogen, its genetic landscape and evolutionary history remain largely unknown. In this study, ten novel complete MCV genome sequences of the two most common MCV genotypes were determined (five MCV1 and five MCV2 sequences) and analyzed together with all MCV complete genomes previously deposited in freely accessible sequence repositories (four MCV1 and a single MCV2). In comparison to MCV1, a higher degree of nucleotide sequence conservation was observed among MCV2 genomes. Large-scale recombination events were identified in two newly assembled MCV1 genomes and one MCV2 genome. One recombination event was located in a newly identified recombinant region of the viral genome, and all previously described recombinant regions were re-identified in at least one novel MCV genome. MCV genes comprising the identified recombinant segments have been previously associated with viral interference with host T-cell and NK-cell immune responses. In conclusion, the two most common MCV genotypes emerged along divergent evolutionary pathways from a common ancestor, and the differences in the heterogeneity of MCV1 and MCV2 populations may be attributed to the strictness of the constraints imposed by the host immune response.

scholarly journals Downregulation of HLA-I by the molluscum contagiosum virus mc080 impacts NK-cell recognition and promotes CD8+ T-cell evasion

2020 ◽  
Vol 101 (8) ◽  
pp. 863-872
Author(s):  
Hana Elasifer ◽  
Eddie C.Y. Wang ◽  
Virginie Prod’homme ◽  
James Davies ◽  
Simone Forbes ◽  
...  
Keyword(s):  
T Cells ◽  
Antigen Processing ◽  
Nk Cell ◽  
Molluscum Contagiosum ◽  
Class I ◽  
Productive Infection ◽  
Cell Surface Expression ◽  
Host Immune System ◽  
Molluscum Contagiosum Virus ◽  
Infected Cells

Molluscum contagiosum virus (MCV) is a common cause of benign skin lesions in young children and currently the only endemic human poxvirus. Following the infection of primary keratinocytes in the epidermis, MCV induces the proliferation of infected cells and this results in the production of wart-like growths. Full productive infection is observed only after the infected cells differentiate. During this prolonged replication cycle the virus must avoid elimination by the host immune system. We therefore sought to investigate the function of the two major histocompatibility complex class-I-related genes encoded by the MCV genes mc033 and mc080. Following insertion into a replication-deficient adenovirus vector, codon-optimized versions of mc033 and mc080 were expressed as endoglycosidase-sensitive glycoproteins that localized primarily in the endoplasmic reticulum. MC080, but not MC033, downregulated cell-surface expression of endogenous classical human leucocyte antigen (HLA) class I and non-classical HLA-E by a transporter associated with antigen processing (TAP)-independent mechanism. MC080 exhibited a capacity to inhibit or activate NK cells in autologous assays in a donor-specific manner. MC080 consistently inhibited antigen-specific T cells being activated by peptide-pulsed targets. We therefore propose that MC080 acts to promote evasion of HLA-I-restricted cytotoxic T cells.

scholarly journals Genetic landscape of recessive diseases in the Vietnamese population from large‐scale clinical exome sequencing

2021 ◽  
Author(s):  
Ngoc Hieu Tran ◽  
Thanh‐Huong Nguyen Thi ◽  
Hung‐Sang Tang ◽  
Le‐Phuc Hoang ◽  
Trung‐Hieu Le Nguyen ◽  
...  
Keyword(s):  
Exome Sequencing ◽  
Large Scale ◽  
Clinical Exome Sequencing ◽  
Genetic Landscape ◽  
Vietnamese Population

scholarly journals Growth of Molluscum Contagiosum Virus in a Human Foreskin Xenograft Model

Virology ◽  
1996 ◽  
Vol 226 (1) ◽  
pp. 95-101 ◽  
Author(s):  
Kenneth H. Fife ◽  
Margot Whitfeld ◽  
Holly Faust ◽  
Michael P. Goheen ◽  
Janine Bryan ◽  
...  
Keyword(s):  
Xenograft Model ◽  
Molluscum Contagiosum ◽  
Molluscum Contagiosum Virus

scholarly journals Abortive Infection and Transformation of Human Embryonic Fibroblasts by Molluscum contagiosum Virus

1974 ◽  
Vol 24 (2) ◽  
pp. 237-246 ◽  
Author(s):  
G. Barbanti-Brodano ◽  
A. Mannini-Palenzona ◽  
O. Varoli ◽  
M. Portolani ◽  
M. La Placa
Keyword(s):  
Molluscum Contagiosum ◽  
Abortive Infection ◽  
Molluscum Contagiosum Virus ◽  
Embryonic Fibroblasts ◽  
Human Embryonic Fibroblasts

Molluscum-contagiosum-Virus in einer Epidermalzyste

2018 ◽  
Vol 16 (9) ◽  
pp. 1144-1146
Author(s):  
Cornelia Sigrid Lissi Müller ◽  
Michael Laue ◽  
Kim Kremer ◽  
Stephanie Becker ◽  
Thomas Vogt ◽  
...  
Keyword(s):  
Molluscum Contagiosum ◽  
Molluscum Contagiosum Virus

PCR assay fails to detect molluscum contagiosum virus-related sequences in AIDS-related Kaposi's sarcoma

1998 ◽  
Vol 73 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Thierry Simonart ◽  
Jean-Christophe Noël ◽  
Jean-Paul Van Vooren ◽  
Philippe Hermans ◽  
Corine Liesnard ◽  
...  
Keyword(s):  
Kaposi's Sarcoma ◽  
Kaposi’S Sarcoma ◽  
Molluscum Contagiosum ◽  
Molluscum Contagiosum Virus ◽  
Pcr Assay

Molluscum contagiosum virus types in genital and non-genital lesions

1989 ◽  
Vol 120 (1) ◽  
pp. 37-41 ◽  
Author(s):  
C.D. PORTER ◽  
N.W. BLAKE ◽  
L.C. ARCHARD ◽  
M.F. MUHLEMANN ◽  
N. ROSEDALE ◽  
...  
Keyword(s):  
Molluscum Contagiosum ◽  
Molluscum Contagiosum Virus

scholarly journals Genomic Landscape and Immunological Profile of Glioblastoma in East Asian Patients

2021 ◽  
Author(s):  
Jean Zhao ◽  
Sheng Zhong ◽  
Bo Wu ◽  
Frank Dubois ◽  
Shanshan Jiang ◽  
...  
Keyword(s):  
East Asian ◽  
Marker Gene ◽  
European Ancestry ◽  
Asian Patients ◽  
Molecular Features ◽  
Immunological Profile ◽  
Genomic Landscape ◽  
Genetic Landscape ◽  
Genetic Features ◽  
East Asian Patients

Abstract While it is well known that Glioblastoma (GBM) shows profound inter- and intra-tumoral heterogeneity, disparities in molecular features across ancestry groups have been largely overlooked. We collected a large cohort of GBM samples from East Asian patients (EAS-GBM) and performed genomic and transcriptomic analyses of these samples (EAS-GBM, n=443). Further characterization and comparative analysis of the EAS-GBM with the predominantly European-ancestry TCGA GBM dataset (EUR-GBM, n=383) revealed differential genomic and genetic landscape and immunological profile of EAS-GBM from EUR-GBM. EAS-GBM showed an enrichment for NF1, H3F3A, TP53 and ATRX mutations compared to EUR-GBM. Transcriptomic clustering revealed distinct EAS-GBM specific subtypes, namely Proliferative (PL), Neuron-Synaptic (NS), Metabolic (MB) and Immunomodulatory (IM). Notably, the classic subtype of EUR-GBMs with EGFR as its main marker gene was absent in EAS-GBMs, Moreover, the IM subgroup in EAS-GBM with an expression profile that has been previously associated with response to immunotherapy in cancers. Together, our comprehensive characterization revealed the unique genomic and genetic features of EAS-GBMs, providing mechanistic rationale in patient stratification for molecular targeted therapy and drug development in the era of personalized medicine.

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