scholarly journals Molecular Characterization and Geographic Distribution of a Mymonavirus in the Population of Botrytis cinerea

Viruses ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 432 ◽  
Author(s):  
Fangmin Hao ◽  
Mingde Wu ◽  
Guoqing Li
Keyword(s):  
Botrytis Cinerea ◽  
Sclerotinia Sclerotiorum ◽  
Cdna Sequence ◽  
Rna Virus ◽  
Open Reading Frames ◽  
Phytopathogenic Fungus ◽  
Rna Dependent Rna Polymerase ◽  
Rdrp Domain ◽  
The Family ◽  
Reading Frames

Here, we characterized a negative single-stranded (−ss)RNA mycovirus, Botrytis cinerea mymonavirus 1 (BcMyV1), isolated from the phytopathogenic fungus Botrytis cinerea. The genome of BcMyV1 is 7863 nt in length, possessing three open reading frames (ORF1–3). The ORF1 encodes a large polypeptide containing a conserved mononegaviral RNA-dependent RNA polymerase (RdRp) domain showing homology to the protein L of mymonaviruses, whereas the possible functions of the remaining two ORFs are still unknown. The internal cDNA sequence (10-7829) of BcMyV1 was 97.9% identical to the full-length cDNA sequence of Sclerotinia sclerotiorum negative stranded RNA virus 7 (SsNSRV7), a virus-like contig obtained from Sclerotinia sclerotiorum metatranscriptomes, indicating BcMyV1 should be a strain of SsNSRV7. Phylogenetic analysis based on RdRp domains showed that BcMyV1 was clustered with the viruses in the family Mymonaviridae, suggesting it is a member of Mymonaviridae. BcMyV1 may be widely distributed in regions where B. cinerea occurs in China and even over the world, although it infected only 0.8% of tested B. cinerea strains.

scholarly journals Complete genome sequence of a novel fusarivirus from the phytopathogenic fungus Corynespora cassiicola

2021 ◽  
Author(s):  
Mingming Liu ◽  
Xintao Liu ◽  
Hui Zhao ◽  
Yunxia Ni ◽  
Min Jia ◽  
...  
Keyword(s):  
Chromosome Segregation ◽  
Open Reading Frames ◽  
Phytopathogenic Fungus ◽  
Corynespora Cassiicola ◽  
Rna Dependent Rna Polymerase ◽  
Rdrp Domain ◽  
Putative Protein ◽  
The Family ◽  
Reading Frames

Abstract Corynespora cassiicola is an important phytopathogenic fungus and it has severely impaired the production of crops. In this study, we report on the molecular characterization of a novel (+) ssRNA mycovirus, Corynespora cassiicola fusarivirus 1 (CcFV1) isolated from C. cassiicola strain 20200826-3-1. Excluding a poly (A) tail, the genome of the virus is 6491 nt containing three putative open reading frames. The large ORF1 encodes a polypeptide of 1524aa with a conserved RNA-dependent RNA polymerase (RdRp) domain, a helicase (Hel) domain, and a Phage-holin-3-6 (Phage-holin) domain. ORF2 encodes a polypeptide with a conserved Chromosome segregation ATPase (Smc) domain. The smallest ORF3 encodes a putative protein with an unknown function. Phylogenetic analysis based on the ORF1 and ORF2 of CcFV1 encoded polypeptide showed that CcFV1 is phylogenetically related to the newly proposed family Fusariviridae. Thus, we suggest that CcFV1 might be a novel member of the family Fusariviridae and is also the first discovered in C. cassiicola.

Molecular characterization of a novel mycovirus isolated from Rhizoctonia solani AG-1 IA 9-11

2021 ◽  
Author(s):  
Yang Sun ◽  
Yan qiong Li ◽  
Wen han Dong ◽  
Ai li Sun ◽  
Ning wei Chen ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Rna Virus ◽  
Dsrna Virus ◽  
Open Reading Frames ◽  
The Family ◽  
Significant Amino Acid Sequence ◽  
Overlapping Open Reading Frames ◽  
Significant Amino Acid ◽  
Reading Frames

Abstract The complete genome of the dsRNA virus isolated from Rhizoctonia solani AG-1 IA 9–11 (designated as Rhizoctonia solani dsRNA virus 11, RsRV11 ) were determined. The RsRV11 genome was 9,555 bp in length, contained three conserved domains, SMC, PRK and RT-like super family, and encoded two non-overlapping open reading frames (ORFs). ORF1 potentially coded for a 204.12 kDa predicted protein, which shared low but significant amino acid sequence identities with the putative protein encoded by Rhizoctonia solani RNA virus HN008 (RsRV-HN008) ORF1. ORF2 potentially coded for a 132.41 kDa protein which contained the conserved motifs of the RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis indicated that RsRV11 was clustered with RsRV-HN008 in a separate clade independent of other virus families. It implies that RsRV11, along with RsRV-HN008 possibly a new fungal virus taxa closed to the family Megabirnaviridae, and RsRV11 is a new member of mycoviruses.

scholarly journals Genome Sequence of Grapevine Virus T, a Novel Foveavirus Infecting Grapevine

2017 ◽  
Vol 5 (37) ◽  
Author(s):  
Yeonhwa Jo ◽  
Myung-Kyu Song ◽  
Hoseong Choi ◽  
Jae-Seong Park ◽  
Jae-Wung Lee ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Rna Virus ◽  
Open Reading Frames ◽  
Grapevine Virus ◽  
The Family ◽  
Putative Member ◽  
Reading Frames

ABSTRACT Here, we report the genome sequence of grapevine virus T (GVT), a novel single-stranded RNA virus identified from a transcriptome of grapevine. The genome of GVT is 8,701 nucleotides in length and encodes five open reading frames. GVT is a putative member of the genus Foveavirus in the family Betaflexiviridae.

scholarly journals First Report of a Mesonivirus and Its Derived Small RNAs in an Aphid Species Aphis citricidus (Hemiptera: Aphididae), Implying Viral Infection Activity

2020 ◽  
Vol 20 (2) ◽  
Author(s):  
Tengyu Chang ◽  
Mengmeng Guo ◽  
Wei Zhang ◽  
Jinzhi Niu ◽  
Jin-Jun Wang
Keyword(s):  
Viral Infection ◽  
Small Rnas ◽  
Rna Virus ◽  
Aphid Species ◽  
Open Reading Frames ◽  
First Report ◽  
The Family ◽  
Interfering Rna ◽  
Unassigned Genus ◽  
Reading Frames

Abstract We report a new positive-sense single-stranded RNA (ss RNA+) virus from the brown citrus aphid Aphis citricidus. The 20,300 nucleotide (nt)-long viral genome contains five open-reading frames and encodes six conserved domains (TM2, 3CLpro, TM3, RdRp, Zm, and HEL1). Phylogenetic analysis and amino acid sequence analysis revealed this virus might belong to an unassigned genus in the family Mesoniviridae. The presence of the virus was also confirmed in the field population. Importantly, analysis of the virus-derived small RNAs showed a 22-nt peak, implying that viral infection triggers the small interfering RNA pathway as antiviral immunity in aphids. This is the first report of a mesonivirus in invertebrates other than mosquitoes.

scholarly journals Molecular Characterization of a Novel Virga-like Virus Isolated From Wheat

2021 ◽  
Author(s):  
Hua Li ◽  
Jun Guo ◽  
ZhongHua Zhao ◽  
Zhuangxin Ye ◽  
Jianping Chen ◽  
...  
Keyword(s):  
Rna Virus ◽  
Open Reading Frames ◽  
Polya Tail ◽  
Rna Dependent Rna Polymerase ◽  
Future Studies ◽  
Putative Protein ◽  
Host Growth ◽  
Positive Sense ◽  
Reading Frames

Abstract In this work, we report the isolation of a novel positive-sense single strand RNA virus from wheat, tentatively named Triticum aestivum-associated virga-like virus 1 (TaAVLV1). Further characterization revealed that the complete genome of TaAVLV1 was divided into two segments, RNA1 and RNA2, which were 3530 and 3466 nt long, excluding the polyA tail. These segments contained two open reading frames (ORFs). The ORF in RNA1 encoded an RNA-dependent RNA polymerase (RdRp), while the ORF in RNA2 encoded a putative protein carrying MET and HEL domains. Phylogenetic analysis based on the RdRp protein of each representative genus of Virgaviridae placed TaAVLV1 in the unclassified Virgaviridae clade of the Virgaviridae family. To our knowledge, this is the first report of virga-like virus isolated from wheat. Future studies will be conducted to examine its effect on host growth and development.

scholarly journals Molecular Characterization of A Novel Victorivirus Infecting Corynespora Cassiicola

2021 ◽  
Author(s):  
Mingming Liu ◽  
Yunxia Ni ◽  
Hui Zhao ◽  
Xintao Liu ◽  
Min Jia ◽  
...  
Keyword(s):  
Genomic Sequence ◽  
Open Reading Frames ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Corynespora Cassiicola ◽  
Rna Dependent Rna Polymerase ◽  
The Family ◽  
Fungal Viruses ◽  
Reading Frames

Abstract One victorivirus was detected in the isolate of Corynespora cassiicola strains 20180909-03, which was named Corynespora cassiicola victorivirus 1 (CcVV1). The whole-genome sequence of the virus was sequenced and identified. The CcVV1 genome is 5140 nt and contains 56.87%GC with two large open reading frames (ORFs) overlapping at the tetranucleotide AUGA. The two ORFs were predicted to encode coat protein (CP) and RNA-dependent RNA polymerase (RdRp) respectively, which were conservative in dsRNA fungal viruses of the family Totiviridae. Conservative domains comparison and phylogenetic analysis of the deduced amino acid sequence of RdRp and CP showed that CcVV1 was a new virus of the Victorivirus genus. As far as we know, it is the first report of a genomic sequence of the genus Victorivirus infecting Corynespora cassiicola.

scholarly journals Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species

Viruses ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 369 ◽  
Author(s):  
Igor Koloniuk ◽  
Thanuja Thekke-Veetil ◽  
Jean-Sébastien Reynard ◽  
Irena Mavrič Pleško ◽  
Jaroslava Přibylová ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 90 ◽  
Open Reading Frames ◽  
Coat Proteins ◽  
Rna Dependent Rna Polymerase ◽  
The Family ◽  
Host Cell Wall ◽  
Reading Frames

Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.

scholarly journals Genome Sequence of Grapevine Virus K, a Novel Vitivirus Infecting Grapevine

2017 ◽  
Vol 5 (37) ◽  
Author(s):  
Yeonhwa Jo ◽  
Myung-Kyu Song ◽  
Hoseong Choi ◽  
Jae-Seong Park ◽  
Jae-Wung Lee ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Rna Virus ◽  
Open Reading Frames ◽  
Grapevine Virus ◽  
The Family ◽  
Putative Member ◽  
Reading Frames

ABSTRACT Here, we report the genome sequence of grapevine virus K (GVK), a novel single-stranded RNA virus identified from a transcriptome of grapevine. The genome of GVK is 7,476 nucleotides in length and encodes 5 open reading frames. GVK is a putative member of the genus Vitivirus in the family Betaflexiviridae.

scholarly journals Molecular Characterization of a Novel Single-stranded RNA Virus, ChRV1, Isolated From the Plant Pathogenic Fungus Colletotrichum Higginsianum

2021 ◽  
Author(s):  
Hong Liu ◽  
Hui Wang ◽  
Xun Lu ◽  
Qian Zhou
Keyword(s):  
Rna Virus ◽  
Pathogenic Fungus ◽  
Gc Content ◽  
Plant Viruses ◽  
Open Reading Frames ◽  
Phytopathogenic Fungus ◽  
Plant Pathogenic Fungus ◽  
Rdrp Domain ◽  
Colletotrichum Higginsianum ◽  
Ssrna Virus

Abstract In this study, a novel single-stranded RNA virus was isolated from the plant pathogenic fungus, Colletotrichum higginsianum strain HTC-5, named “Colletotrichum higginsianum ssRNA virus 1” (ChRV1). The complete genome of ChRV1 is 3850 bp in length with a GC content of 52 % and encodes two in-frame open reading frames (ORFs): ORF1 (smaller) and ORF2 (larger). ORF1 encodes a protein with highest similarity to proteins encoded by Phoma matteucciicola RNA virus 1 (PmRV1, 47.99% identity) and Periconia macrospinosa ambiguivirus 1 (PmAV1, 50.73% identity). ORF2 encodes a protein with a conserved RNA-dependent RNA polymerase (RdRp) domain with similarity to RdRps of PmRV1 (61.41% identity) and PmAV1 (60.61% identity), which are unclassified (+)ssRNA mycoviruses reported recently. Phylogenetic analysis of the RdRp domain suggested that ChRV1 grouped together with PmRV1, PmAV1 and other unclassified (+)ssRNA mycoviruses, and had a distant relationship to invertebrate viruses and plant viruses of the family Tombusviridae. This is the first report of a novel (+)ssRNA virus infecting the phytopathogenic fungus C. higginsianum.

scholarly journals RNA-Dependent RNA Polymerase from Heterobasidion RNA Virus 6 Is an Active Replicase In Vitro

Viruses ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1738
Author(s):  
Alesia A. Levanova ◽  
Eeva J. Vainio ◽  
Jarkko Hantula ◽  
Minna M. Poranen
Keyword(s):  
Rna Polymerase ◽  
Rna Synthesis ◽  
Rna Virus ◽  
Polymerization Reaction ◽  
Rna Dependent Rna Polymerase ◽  
Independent Manner ◽  
Nucleotidyl Transferase ◽  
Rna Polymerization ◽  
The Family

Heterobasidion RNA virus 6 (HetRV6) is a double-stranded (ds)RNA mycovirus and a member of the recently established genus Orthocurvulavirus within the family Orthocurvulaviridae. The purpose of the study was to determine the biochemical requirements for RNA synthesis catalyzed by HetRV6 RNA-dependent RNA polymerase (RdRp). HetRV6 RdRp was expressed in Escherichia coli and isolated to near homogeneity using liquid chromatography. The enzyme activities were studied in vitro using radiolabeled UTP. The HetRV6 RdRp was able to initiate RNA synthesis in a primer-independent manner using both virus-related and heterologous single-stranded (ss)RNA templates, with a polymerization rate of about 46 nt/min under optimal NTP concentration and temperature. NTPs with 2′-fluoro modifications were also accepted as substrates in the HetRV6 RdRp-catalyzed RNA polymerization reaction. HetRV6 RdRp transcribed viral RNA genome via semi-conservative mechanism. Furthermore, the enzyme demonstrated terminal nucleotidyl transferase (TNTase) activity. Presence of Mn2+ was required for the HetRV6 RdRp catalyzed enzymatic activities. In summary, our study shows that HetRV6 RdRp is an active replicase in vitro that can be potentially used in biotechnological applications, molecular biology, and biomedicine.

Sign in / Sign up

Export Citation Format

Share Document