scholarly journals Molecular Characterization of Divergent Closterovirus Isolates Infecting Ribes Species

Viruses ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 369 ◽  
Author(s):  
Igor Koloniuk ◽  
Thanuja Thekke-Veetil ◽  
Jean-Sébastien Reynard ◽  
Irena Mavrič Pleško ◽  
Jaroslava Přibylová ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 90 ◽  
Open Reading Frames ◽  
Coat Proteins ◽  
Rna Dependent Rna Polymerase ◽  
The Family ◽  
Host Cell Wall ◽  
Reading Frames

Five isolates of a new member of the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in the currant. The 17-kb-long genome codes for 10 putative proteins. The replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code for a small protein predicted to integrate into the host cell wall, a heat-shock protein 70 homolog, a heat-shock protein 90 homolog, two coat proteins, and three proteins of unknown functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus, whereas recombination analysis provided evidence of intraspecies recombination.

scholarly journals Molecular characterization of divergent closterovirus isolates infecting Ribes species

2018 ◽  
Author(s):  
Igor Koloniuk ◽  
Thanuja Thekke-Veetil ◽  
Jean-Sébastien Reynard ◽  
Irena Mavrič Pleško ◽  
Jaroslava Přibylová ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 90 ◽  
Open Reading Frames ◽  
Accessory Proteins ◽  
Rna Dependent Rna Polymerase ◽  
Polymerase Domain ◽  
Putative Member ◽  
Reading Frames

Five isolates of a new putative member of the genus Closterovirus, tentatively named blackcurrant leafroll associated virus 1 (BcLRaV-1), were identified in currant. The 17 kb long genome of BcLRaV-1 contained 10 open reading frames (ORFs). The replication associated polyprotein has two papain-like leader proteases, a methyltransferase, a helicase and an RNA-dependent RNA polymerase domain. Additional ORFs coded for heat shock protein 70 homolog, heat shock protein 90 homolog, two divergent copies of coat protein, and three accessory proteins without identifiable functions. Phylogenetic analysis showed that BcLRaV-1 is related to members of the genus Closterovirus and recombination analysis of the isolates showed clear evidences of intraspecies recombination.

scholarly journals Molecular Characterization of A Novel Victorivirus Infecting Corynespora Cassiicola

2021 ◽  
Author(s):  
Mingming Liu ◽  
Yunxia Ni ◽  
Hui Zhao ◽  
Xintao Liu ◽  
Min Jia ◽  
...  
Keyword(s):  
Genomic Sequence ◽  
Open Reading Frames ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Corynespora Cassiicola ◽  
Rna Dependent Rna Polymerase ◽  
The Family ◽  
Fungal Viruses ◽  
Reading Frames

Abstract One victorivirus was detected in the isolate of Corynespora cassiicola strains 20180909-03, which was named Corynespora cassiicola victorivirus 1 (CcVV1). The whole-genome sequence of the virus was sequenced and identified. The CcVV1 genome is 5140 nt and contains 56.87%GC with two large open reading frames (ORFs) overlapping at the tetranucleotide AUGA. The two ORFs were predicted to encode coat protein (CP) and RNA-dependent RNA polymerase (RdRp) respectively, which were conservative in dsRNA fungal viruses of the family Totiviridae. Conservative domains comparison and phylogenetic analysis of the deduced amino acid sequence of RdRp and CP showed that CcVV1 was a new virus of the Victorivirus genus. As far as we know, it is the first report of a genomic sequence of the genus Victorivirus infecting Corynespora cassiicola.

scholarly journals Complete genome sequence of a novel fusarivirus from the phytopathogenic fungus Corynespora cassiicola

2021 ◽  
Author(s):  
Mingming Liu ◽  
Xintao Liu ◽  
Hui Zhao ◽  
Yunxia Ni ◽  
Min Jia ◽  
...  
Keyword(s):  
Chromosome Segregation ◽  
Open Reading Frames ◽  
Phytopathogenic Fungus ◽  
Corynespora Cassiicola ◽  
Rna Dependent Rna Polymerase ◽  
Rdrp Domain ◽  
Putative Protein ◽  
The Family ◽  
Reading Frames

Abstract Corynespora cassiicola is an important phytopathogenic fungus and it has severely impaired the production of crops. In this study, we report on the molecular characterization of a novel (+) ssRNA mycovirus, Corynespora cassiicola fusarivirus 1 (CcFV1) isolated from C. cassiicola strain 20200826-3-1. Excluding a poly (A) tail, the genome of the virus is 6491 nt containing three putative open reading frames. The large ORF1 encodes a polypeptide of 1524aa with a conserved RNA-dependent RNA polymerase (RdRp) domain, a helicase (Hel) domain, and a Phage-holin-3-6 (Phage-holin) domain. ORF2 encodes a polypeptide with a conserved Chromosome segregation ATPase (Smc) domain. The smallest ORF3 encodes a putative protein with an unknown function. Phylogenetic analysis based on the ORF1 and ORF2 of CcFV1 encoded polypeptide showed that CcFV1 is phylogenetically related to the newly proposed family Fusariviridae. Thus, we suggest that CcFV1 might be a novel member of the family Fusariviridae and is also the first discovered in C. cassiicola.

Molecular characterization of a novel mycovirus isolated from Rhizoctonia solani AG-1 IA 9-11

2021 ◽  
Author(s):  
Yang Sun ◽  
Yan qiong Li ◽  
Wen han Dong ◽  
Ai li Sun ◽  
Ning wei Chen ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Rna Virus ◽  
Dsrna Virus ◽  
Open Reading Frames ◽  
The Family ◽  
Significant Amino Acid Sequence ◽  
Overlapping Open Reading Frames ◽  
Significant Amino Acid ◽  
Reading Frames

Abstract The complete genome of the dsRNA virus isolated from Rhizoctonia solani AG-1 IA 9–11 (designated as Rhizoctonia solani dsRNA virus 11, RsRV11 ) were determined. The RsRV11 genome was 9,555 bp in length, contained three conserved domains, SMC, PRK and RT-like super family, and encoded two non-overlapping open reading frames (ORFs). ORF1 potentially coded for a 204.12 kDa predicted protein, which shared low but significant amino acid sequence identities with the putative protein encoded by Rhizoctonia solani RNA virus HN008 (RsRV-HN008) ORF1. ORF2 potentially coded for a 132.41 kDa protein which contained the conserved motifs of the RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis indicated that RsRV11 was clustered with RsRV-HN008 in a separate clade independent of other virus families. It implies that RsRV11, along with RsRV-HN008 possibly a new fungal virus taxa closed to the family Megabirnaviridae, and RsRV11 is a new member of mycoviruses.

scholarly journals Molecular Characterization and Geographic Distribution of a Mymonavirus in the Population of Botrytis cinerea

Viruses ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 432 ◽  
Author(s):  
Fangmin Hao ◽  
Mingde Wu ◽  
Guoqing Li
Keyword(s):  
Botrytis Cinerea ◽  
Sclerotinia Sclerotiorum ◽  
Cdna Sequence ◽  
Rna Virus ◽  
Open Reading Frames ◽  
Phytopathogenic Fungus ◽  
Rna Dependent Rna Polymerase ◽  
Rdrp Domain ◽  
The Family ◽  
Reading Frames

Here, we characterized a negative single-stranded (−ss)RNA mycovirus, Botrytis cinerea mymonavirus 1 (BcMyV1), isolated from the phytopathogenic fungus Botrytis cinerea. The genome of BcMyV1 is 7863 nt in length, possessing three open reading frames (ORF1–3). The ORF1 encodes a large polypeptide containing a conserved mononegaviral RNA-dependent RNA polymerase (RdRp) domain showing homology to the protein L of mymonaviruses, whereas the possible functions of the remaining two ORFs are still unknown. The internal cDNA sequence (10-7829) of BcMyV1 was 97.9% identical to the full-length cDNA sequence of Sclerotinia sclerotiorum negative stranded RNA virus 7 (SsNSRV7), a virus-like contig obtained from Sclerotinia sclerotiorum metatranscriptomes, indicating BcMyV1 should be a strain of SsNSRV7. Phylogenetic analysis based on RdRp domains showed that BcMyV1 was clustered with the viruses in the family Mymonaviridae, suggesting it is a member of Mymonaviridae. BcMyV1 may be widely distributed in regions where B. cinerea occurs in China and even over the world, although it infected only 0.8% of tested B. cinerea strains.

Purification of heat shock protein 90 from calf uterus and rat liver and characterization of the highly hydrophobic region

1989 ◽  
Vol 992 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Masaomi Iwasaki ◽  
Hiroyuki Saito ◽  
Masahide Yamamoto ◽  
Kenneth S. Korach ◽  
Tsuneyoshi Hirogome ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Rat Liver ◽  
Heat Shock Protein 90 ◽  
Hydrophobic Region ◽  
Highly Hydrophobic

scholarly journals Molecular Characterization of a Novel Virga-like Virus Isolated From Wheat

2021 ◽  
Author(s):  
Hua Li ◽  
Jun Guo ◽  
ZhongHua Zhao ◽  
Zhuangxin Ye ◽  
Jianping Chen ◽  
...  
Keyword(s):  
Rna Virus ◽  
Open Reading Frames ◽  
Polya Tail ◽  
Rna Dependent Rna Polymerase ◽  
Future Studies ◽  
Putative Protein ◽  
Host Growth ◽  
Positive Sense ◽  
Reading Frames

Abstract In this work, we report the isolation of a novel positive-sense single strand RNA virus from wheat, tentatively named Triticum aestivum-associated virga-like virus 1 (TaAVLV1). Further characterization revealed that the complete genome of TaAVLV1 was divided into two segments, RNA1 and RNA2, which were 3530 and 3466 nt long, excluding the polyA tail. These segments contained two open reading frames (ORFs). The ORF in RNA1 encoded an RNA-dependent RNA polymerase (RdRp), while the ORF in RNA2 encoded a putative protein carrying MET and HEL domains. Phylogenetic analysis based on the RdRp protein of each representative genus of Virgaviridae placed TaAVLV1 in the unclassified Virgaviridae clade of the Virgaviridae family. To our knowledge, this is the first report of virga-like virus isolated from wheat. Future studies will be conducted to examine its effect on host growth and development.

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