scholarly journals Analysis of Asymmetric Cell Division Using Human Neuroblastoma Cell Lines as a Model System

Symmetry ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1907
Author(s):  
Hideki Izumi ◽  
Yasuhiko Kaneko ◽  
Akira Nakagawara
Keyword(s):  
Cell Division ◽  
Cell Lines ◽  
Asymmetric Cell Division ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cells ◽  
Model System ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Neuroblastoma Cell Lines

Neuroblastoma is one of the most common childhood solid tumors and develops from neural stem cells that normally comprise the embryonic structure termed the neural crest. Human neuroblastoma cell lines have special properties as they exhibit cell growth and are induced to become mature neurons by drugs such as retinoid. Therefore, we examined asymmetric cell division (ACD) using human neuroblastoma cells as an ACD model, and confirmed that ACD in human cancer cells is evolutionally conserved. Furthermore, we demonstrated that MYCN is involved in cell division fate. We introduce the brief history of ACD study using neuroblastoma cell lines and discuss why human neuroblastoma cells are an ideal model system for clarifying the mechanism of ACD.

scholarly journals Regulation of c-myb expression in human neuroblastoma cells during retinoic acid-induced differentiation.

1988 ◽  
Vol 8 (4) ◽  
pp. 1677-1683 ◽  
Author(s):  
C J Thiele ◽  
P S Cohen ◽  
M A Israel
Keyword(s):  
Retinoic Acid ◽  
Fetal Development ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Hematopoietic Lineage ◽  
Specific Manner ◽  
Neuroblastoma Cell Lines

We detected expression of the c-myb proto-oncogene, which was initially thought to be expressed in a tissue-specific manner in cells of hematopoietic lineage, in human tissues of neuronal origin. Since the level of c-myb expression declined during fetal development, we studied the regulation of its expression in human neuroblastoma cell lines induced to differentiate by retinoic acid. The expression of c-myb declined during the maturation of neuroblastoma cells, and this change was mediated by a decrease in c-myb transcription.

scholarly journals Cell Line-Dependent Variability of Coordinate Expression of p75NTR and CRABP1 and Modulation of Effects of Fenretinide on Neuroblastoma Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Yaoli Pu Yang ◽  
Simeng Wang ◽  
Xingguo Li ◽  
Nina F. Schor
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cells ◽  
Neurotrophin Receptor ◽  
Human Neuroblastoma Cell ◽  
P75 Neurotrophin Receptor ◽  
Human Neuroblastoma ◽  
Neuroblastoma Cell Lines

Neuroblastoma is a childhood neural crest tumor. Fenretinide, a retinoic acid analogue, induces accumulation of mitochondrial reactive oxygen species and consequent apoptosis in neuroblastoma cells. The p75 neurotrophin receptor (p75NTR) enhances the antineuroblastoma cell efficacy of fenretinidein vitro. We examined the role of the retinoid binding protein, CRABP1, in p75NTR-mediated potentiation of the efficacy of fenretinide. Knockdown and overexpression, respectively, of either p75NTR or CRABP1 were effected in neuroblastoma cell lines using standard techniques. Expression was determined by qRT-PCR and confirmed at the protein level by Western blot. Metabolic viability was determined by Alamar blue assay. While protein content of CRABP1 correlated roughly with that of p75NTR in the three neuroblastoid or epithelioid human neuroblastoma cell lines studied, manipulation of p75NTR expression resulted in cell line-dependent, variable change in CRABP1 expression. Furthermore, in some cell lines, induced expression of CRABP1 in the absence of p75NTR did not alter cell sensitivity to fenretinide treatment. The effects of manipulation of p75NTR expression on CRABP1 expression and the effects of CRABP1 expression on fenretinide efficacy are therefore neuroblastoma cell line-dependent. Potentiation of the antineuroblastoma cell effects of fenretinide by p75NTR is not mediated solely through CRABP1.

Regulation of c-myb expression in human neuroblastoma cells during retinoic acid-induced differentiation

1988 ◽  
Vol 8 (4) ◽  
pp. 1677-1683 ◽  
Author(s):  
C J Thiele ◽  
P S Cohen ◽  
M A Israel
Keyword(s):  
Retinoic Acid ◽  
Fetal Development ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Hematopoietic Lineage ◽  
Specific Manner ◽  
Neuroblastoma Cell Lines

We detected expression of the c-myb proto-oncogene, which was initially thought to be expressed in a tissue-specific manner in cells of hematopoietic lineage, in human tissues of neuronal origin. Since the level of c-myb expression declined during fetal development, we studied the regulation of its expression in human neuroblastoma cell lines induced to differentiate by retinoic acid. The expression of c-myb declined during the maturation of neuroblastoma cells, and this change was mediated by a decrease in c-myb transcription.

Delta opioid receptor in human neuroblastoma cell lines

1986 ◽  
Vol 382 (2) ◽  
pp. 327-331 ◽  
Author(s):  
Günther Hochhaus ◽  
Victor C. Yu ◽  
Wolfgang Sadée
Keyword(s):  
Opioid Receptor ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Delta Opioid Receptor ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma ◽  
Neuroblastoma Cell Lines

Ciliary neurotrophic factor-induced gene expression in human neuroblastoma cell lines

1995 ◽  
Vol 20 (6) ◽  
pp. 675-680 ◽  
Author(s):  
P. Rossino ◽  
G. Volpe ◽  
A. Negro ◽  
L. Callegaro ◽  
F. Altruda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Neurotrophic Factor ◽  
Neuroblastoma Cell ◽  
Ciliary Neurotrophic Factor ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma ◽  
Neuroblastoma Cell Lines

Betulinic acid induces apoptosis in human neuroblastoma cell lines

1997 ◽  
Vol 33 (12) ◽  
pp. 2007-2010 ◽  
Author(s):  
M.L Schmidt ◽  
K.L Kuzmanoff ◽  
L Ling-Indeck ◽  
J.M Pezzuto
Keyword(s):  
Cell Lines ◽  
Betulinic Acid ◽  
Neuroblastoma Cell ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma ◽  
Neuroblastoma Cell Lines
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