scholarly journals Evaluation of Symbiotic Association between Various Rhizobia, Capable of Producing Plant-Growth-Promoting Biomolecules and Mung Bean for Sustainable Production

2021 ◽  
Vol 13 (24) ◽  
pp. 13832
Author(s):  
Abid Mahmood ◽  
Tanvir Shahzad ◽  
Sabir Hussain ◽  
Qasim Ali ◽  
Hayssam M. Ali ◽  
...  
Keyword(s):  
Plant Growth ◽  
Mung Bean ◽  
Total Phenolics ◽  
Fresh Mass ◽  
Symbiotic Association ◽  
Rhizobial Strain ◽  
Plant Growth Promoting ◽  
Uninoculated Control ◽  
Growth Promoting ◽  
Rhizobial Isolates

To feed the increased world population, sustainability in the production of crops is the need of the hour, and exploration of an effective symbiotic association of rhizobia with legumes may serve the purpose. A laboratory-scale experiment was conducted to evaluate the symbiotic effectiveness of twenty wild rhizobial isolates (MR1–MR20) on the growth, physiology, biochemical traits, and nodulation of mung bean to predict better crop production with higher yields. Rhizobial strain MR4 resulted in a 52% increase in shoot length and 49% increase in shoot fresh mass, while MR5 showed a 30% increase in root length, with 67% and 65% improvement in root fresh mass by MR4 and MR5, respectively, compared to uninoculated control. Total dry matter of mung bean was enhanced by 73% and 68% with strains MR4 and MR5 followed by MR1 and MR3 with 60% increase in comparison to control. Rhizobial strain MR5 produced a maximum (25 nodules) number of nodules followed by MR4, MR3, and MR1 which produced 24, 23, and 21 nodules per plant. Results related to physiological parameters showed the best performance of MR4 and MR5 compared to control among all treatments. MR4 strain helped the plants to produce the lowest values of total soluble protein (TSP) (38% less), flavonoids contents (44% less), and malondialdehyde (MDA) contents (52% less) among all treatments compared to uninoculated control plants. Total phenolics contents of mung bean plants also showed significantly variable results, with the highest value of 54.79 mg kg−1 in MR—inoculated plants, followed by MR5- and MR1-inoculated plants, while the minimum concentration of total phenolics was recorded in uninoculated control plants of mung bean. Based on the results of growth promotion, nodulation ability, and physiological and biochemical characteristics recorded in an experimental trial conducted under gnotobiotic conditions, four rhizobial isolates (MR1, MR3, MR4, and MR5) were selected using cluster and principal component analysis. Selected strains were also tested for a variety of plant-growth-promoting molecules to develop a correlation with the results of plant-based parameters, and it was concluded that these wild rhizobial strains were effective in improving sustainable production of mung bean.

scholarly journals Significance of Plant Growth Promoting Rhizobacteria in Grain Legumes: Growth Promotion and Crop Production

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1596
Author(s):  
Karivaradharajan Swarnalakshmi ◽  
Vandana Yadav ◽  
Deepti Tyagi ◽  
Dolly Wattal Dhar ◽  
Annapurna Kannepalli ◽  
...  
Keyword(s):  
Plant Growth ◽  
Mycorrhizal Fungi ◽  
Plant Growth Promoting Rhizobacteria ◽  
Grain Legumes ◽  
Growth Stages ◽  
Symbiotic Association ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Leguminous Crops ◽  
And Function

Grain legumes are an important component of sustainable agri-food systems. They establish symbiotic association with rhizobia and arbuscular mycorrhizal fungi, thus reducing the use of chemical fertilizers. Several other free-living microbial communities (PGPR—plant growth promoting rhizobacteria) residing in the soil-root interface are also known to influence biogeochemical cycles and improve legume productivity. The growth and function of these microorganisms are affected by root exudate molecules secreted in the rhizosphere region. PGPRs produce the chemicals which stimulate growth and functions of leguminous crops at different growth stages. They promote plant growth by nitrogen fixation, solubilization as well as mineralization of phosphorus, and production of phytohormone(s). The co-inoculation of PGPRs along with rhizobia has shown to enhance nodulation and symbiotic interaction. The recent molecular tools are helpful to understand and predict the establishment and function of PGPRs and plant response. In this review, we provide an overview of various growth promoting mechanisms of PGPR inoculations in the production of leguminous crops.

scholarly journals Deciphering the Potential of Rhizobium pusense MB-17a, a Plant Growth-Promoting Root Endophyte, and Functional Annotation of the Genes Involved in the Metabolic Pathway

2021 ◽  
Vol 8 ◽  
Author(s):  
Twinkle Chaudhary ◽  
Rajesh Gera ◽  
Pratyoosh Shukla
Keyword(s):  
Plant Growth ◽  
Mung Bean ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Endophytic Bacterium ◽  
Fresh Weight ◽  
Root Endophyte ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

Plant growth-promoting rhizobacteria (PGPR) are root endophytic bacteria used for growth promotion, and they have broader applications in enhancing specific crop yield as a whole. In the present study, we have explored the potential of Rhizobium pusense MB-17a as an endophytic bacterium isolated from the roots of the mung bean (Vigna radiata) plant. Furthermore, this bacterium was sequenced and assembled to reveal its genomic potential associated with plant growth-promoting traits. Interestingly, the root endophyte R. pusense MB-17a showed all essential PGPR traits which were determined by biochemical and PGPR tests. It was noted that this root endophytic bacterium significantly produced siderophores, indole acetic acid (IAA), ammonia, and ACC deaminase and efficiently solubilized phosphate. The maximum IAA and ammonia produced were observed to be 110.5 and 81 μg/ml, respectively. Moreover, the PGPR potential of this endophytic bacterium was also confirmed by a pot experiment for mung bean (V. radiata), whose results show a substantial increase in the plant's fresh weight by 76.1% and dry weight by 76.5% on the 60th day after inoculation of R. pusense MB-17a. Also, there is a significant enhancement in the nodule number by 66.1% and nodule fresh weight by 162% at 45th day after inoculation with 100% field capacity after the inoculation of R. pusense MB-17a. Besides this, the functional genomic annotation of R. pusense MB-17a determined the presence of different proteins and transporters that are responsible for its stress tolerance and its plant growth-promoting properties. It was concluded that the unique presence of genes like rpoH, otsAB, and clpB enhances the symbiosis process during adverse conditions in this endophyte. Through Rapid Annotation using Subsystem Technology (RAST) analysis, the key genes involved in the production of siderophores, volatile compounds, indoles, nitrogenases, and amino acids were also predicted. In conclusion, the strain described in this study gives a novel idea of using such type of endophytes for improving plant growth-promoting traits under different stress conditions for sustainable agriculture.

Evaluation of plant growth promoting rhizobacteria (PGPR) for the control of charcoal rot of Mung bean

2021 ◽  
Vol 54 (1) ◽  
Author(s):  
Amjid Khan ◽  
Shehzad Asad ◽  
Asghari Bano ◽  
Rashid Abbas Khan ◽  
Tauqeer Ahmed Qadri
Keyword(s):  
Plant Growth ◽  
Mung Bean ◽  
Plant Growth Promoting Rhizobacteria ◽  
Charcoal Rot ◽  
Plant Growth Promoting ◽  
Growth Promoting

Growth and Nutrient Uptake of Orchardgrass (Dactylis glomerata L.) and Meadow Fescue (Festuca pratensis Huds.) as Affected by Rhizobacteria

2016 ◽  
Vol 44 (1) ◽  
pp. 296-301 ◽  
Author(s):  
Olivera STAJKOVIĆ-SRBINOVIĆ ◽  
Dušica DELIĆ ◽  
Djordje KUZMANOVIĆ ◽  
Biljana SIKIRIĆ ◽  
Nataša RASULIĆ ◽  
...  
Keyword(s):  
Plant Growth ◽  
Dactylis Glomerata ◽  
Grass Species ◽  
Festuca Pratensis ◽  
Meadow Fescue ◽  
Total N ◽  
Plant Growth Promoting ◽  
Uninoculated Control ◽  
Growth Promoting ◽  
Dactylis Glomerata L

A diverse group of soil bacteria found in the rhizosphere which can colonize plant roots and improve plant growth are designated as plant growth promoting rhizobacteria. The aim of this study was isolation and screening of different rhizobacterial strains for plant growth promoting characteristics and their ability to improve growth of two grass species, orchardgrass (Dactylis glomerata L.) and meadow fescue (Festuca pratensis Huds.). The strains investigated, belonging to the genera Azotobacter, Bacillus, Pseudomonas and rhizobial bacteria, showed various plant growth promoting traits, such as phosphate solubilisation, siderophore production, and indole-3-acetic acid (IAA) production. Co-inoculation of meadow fescue with Azotobacter chroococcum A2 and Sinorhizobium meliloti or Pseudomonas sp., and A. chroococcum A5 with S. meliloti, significantly increased shoot dry weight (SDW)(25-33%), as well as total N (26-33%), P (24-31%) and K (26-28%) contents in plants (mg pot-1), compared to uninoculated control. In addition, inoculation of orchardgrass with A. chroococcum strain A1, as well as co-inoculation with B. megaterium and A. chroococcum A1 or A31, significantly increased SDW (51-59%) and total N (54-59%), P (51-74%) and K (49-55%) contents, compared to uninoculated control. Nitrogen percentage in SDW was slightly higher than sufficiency ranges, while K percentage was optimal in all treatments in both species. Phosphorous percentage was lower than sufficiency ranges as a consequence of very low soil P content. The results emphasize the potential of particular rhizobacteria to improve the growth of forage grasses.

scholarly journals Genome Sequencing of a Mung Bean Plant Growth Promoting Strain ofP. aeruginosawith Biocontrol Ability

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Devaraj Illakkiam ◽  
Manoharan Shankar ◽  
Paramasivan Ponraj ◽  
Jeyaprakash Rajendhran ◽  
Paramasamy Gunasekaran
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Genome Sequencing ◽  
Mung Bean ◽  
Draft Genome ◽  
Hydrolytic Enzymes ◽  
Personal Genome ◽  
Cellulose Synthesis ◽  
Plant Growth Promoting ◽  
Growth Promoting

Pseudomonas aeruginosaPGPR2 is a mung bean rhizosphere strain that produces secondary metabolites and hydrolytic enzymes contributing to excellent antifungal activity againstMacrophomina phaseolina, one of the prevalent fungal pathogens of mung bean. Genome sequencing was performed using the Ion Torrent Personal Genome Machine generating 1,354,732 reads (6,772,433 sequenced bases) achieving ~25-fold coverage of the genome. Reference genome assembly using MIRA 3.4.0 yielded 198 contigs. The draft genome of PGPR2 encoded 6803 open reading frames, of which 5314 were genes with predicted functions, 1489 were genes of known functions, and 80 were RNA-coding genes. Strain specific and core genes ofP. aeruginosaPGPR2 that are relevant to rhizospheric habitat were identified by pangenome analysis. Genes involved in plant growth promoting function such as synthesis of ACC deaminase, indole-3-acetic acid, trehalose, mineral scavenging siderophores, hydrogen cyanide, chitinases, acyl homoserine lactones, acetoin, 2,3-butanediol, and phytases were identified. In addition, niche-specific genes such as phosphate solubilising 3-phytase, adhesins, pathway-specific transcriptional regulators, a diguanylate cyclase involved in cellulose synthesis, a receptor for ferrienterochelin, a DEAD/DEAH-box helicase involved in stress tolerance, chemotaxis/motility determinants, an HtpX protease, and enzymes involved in the production of a chromanone derivative with potent antifungal activity were identified.

Sign in / Sign up

Export Citation Format

Share Document