scholarly journals Comparative Analysis of Biological Effects Induced on Different Cell Types by Magnetic Fields with Magnetic Flux Densities in the Range of 1–60 mT and Frequencies up to 50 Hz

2018 ◽  
Vol 10 (8) ◽  
pp. 2776 ◽  
Author(s):  
Cristian Vergallo ◽  
Luciana Dini
Keyword(s):  
Magnetic Fields ◽  
Magnetic Flux ◽  
Transmission Lines ◽  
Bystander Effect ◽  
Human Peripheral Blood ◽  
Biological Effects ◽  
Low Frequency ◽  
Cell Types ◽  
Peripheral Blood Lymphocytes ◽  
Different Cell Types

Moderate static magnetic fields (SMFs) are generated from sources such as new-generation electric trams and trains, electric arc welding, and magnetic resonance imaging (MRI) devices, as well as during the industrial production of aluminium, while extremely low frequency pulsed magnetic fields (ELF-PMFs) are produced by house power installations, household appliances, and high voltages transmission lines. Moderate SMFs and ELF-PMFs with magnetic flux densities (B) in the range of 1–60 mT and frequencies (f) up to 50 Hz are common MF exposure sources for the population. Even though humans are continually exposed to these MFs, to date no definitive endpoint has been drawn about their safety. In this review, the state of knowledge about the biological effects induced by these MFs on different cell types will be addressed. In our own observation, the putative modulation of Ca2+/H+ and Na+/H+ plasma membrane antiporters of human peripheral blood lymphocytes (PBLs) was found to occur after a 24 h exposure to a 6 mT SMF, and the bystander effect observed on U937 cells cultivated for up to 6 h in the conditioned medium harvested from human PBLs previously exposed for 24 h to the same MF (secondary necrosis induction) will be also herein discussed.

Biological effects of 6 mT static magnetic fields: A comparative study in different cell types

2006 ◽  
Vol 27 (7) ◽  
pp. 560-577 ◽  
Author(s):  
Bernadette Tenuzzo ◽  
Alfonsina Chionna ◽  
Elisa Panzarini ◽  
Remigio Lanubile ◽  
Patrizia Tarantino ◽  
...  
Keyword(s):  
Magnetic Fields ◽  
Comparative Study ◽  
Biological Effects ◽  
Cell Types ◽  
Static Magnetic Fields ◽  
Different Cell Types

scholarly journals Biological Effects of a Low-Frequency Electromagnetic Field on Yeast Cells of the Genus Saccharomyces Cerevisiae

2021 ◽  
Vol 21 (2) ◽  
pp. 34-41
Author(s):  
K Sladicekova ◽  
M Bereta ◽  
J Misek ◽  
D Parizek ◽  
J Jakus
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Electromagnetic Field ◽  
Magnetic Flux ◽  
Flux Density ◽  
Biological Effects ◽  
Low Frequency ◽  
Growth Dynamics ◽  
Yeast Cells ◽  
Magnetic Flux Density ◽  
Time Points

Abstract Background: Although the scientific community is extensively concerned with the effects of the EMF, the unambiguous explanation of its effects on living structures is still lacking. Goals: The goal of the study was to evaluate the effect of a low-frequency (LF) electromagnetic field (EMF) on the growth and multiplication of the yeast Saccharomyces cerevisiae. Methods: Yeast cells were exposed to a frequency of 900 Hz and a magnetic flux density of 2.3 mT. The duration of each experiment was 8 hours, in the beginning of the measurement the value of frequency, rms (root mean square) value of electric current (2 A), and magnetic flux density were fixed set on the exposure device. A paired experiment was performed, a sample exposed to EMF, and a sample shielded from the field. Subsequently, samples were taken every two hours, the number of cells was recorded, and then the concentration of the yeast cells was evaluated at time points. The time points reflected the exposure time of the samples exposed to EMF. Results: The results indicate that LF EMF at given parameters has an inhibitory effect on the growth and multiplication of yeast cells. Conclusion: Exposure to EMF can cause the differences in growth dynamics between cells exposed to the field and the unexposed ones.

scholarly journals Methods for Obtaining Purified Lymphocytes, Glass-adherent Mononuclear Cells, and a Population Containing Both Cell Types From Human Peripheral Blood

Blood ◽  
1972 ◽  
Vol 40 (1) ◽  
pp. 77-89 ◽  
Author(s):  
William R. Levis ◽  
Jay H. Robbins
Keyword(s):  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Human Leukocyte ◽  
Culture Fluid ◽  
Cell Types ◽  
Peripheral Blood Lymphocytes ◽  
Limiting Factor ◽  
Lymphocyte Populations ◽  
Two Populations

Abstract Methods are presented for obtaining simultaneously or separately two populations of cells from human peripheral blood, lymphocytes and monocytes, both of which are required to obtain blastogenesis and DNA synthesis in human leukocyte cultures. A simple 5-min centrifugation of heparinized blood yields a mononuclear leukocyte culture fluid containing 70-90% lymphocytes with few granulocytes but with sufficient numbers of monocytes so that the latter cell is not a limiting factor in the blastogenic reaction. A method is also presented for removing both granulocytes and monocytes from lymphocyte populations in a manner that permits monitoring and choice of the degree of lymphocyte purification. A method is also presented for obtaining glass-adherent mononuclear cells that do not undergo blastogenesis but will enable suitably stimulated "purified" lymphocytes to undergo blastogenesis. Studies of the function and morphology of these different cell populations are presented.

scholarly journals Induction of micronuclei and superoxide production in neuroblastoma and glioma cell lines exposed to weak 50 Hz magnetic fields

2016 ◽  
Vol 13 (114) ◽  
pp. 20150995 ◽  
Author(s):  
Kavindra Kumar Kesari ◽  
Jukka Juutilainen ◽  
Jukka Luukkonen ◽  
Jonne Naarala
Keyword(s):  
Magnetic Fields ◽  
Cell Lines ◽  
Biological Effects ◽  
Low Frequency ◽  
Epidemiological Studies ◽  
Dna Integrity ◽  
C6 Cells ◽  
Adverse Health Effects ◽  
Mitochondrial Superoxide ◽  
Exposure Response

Extremely low-frequency (ELF) magnetic fields (MF) have been associated with adverse health effects in epidemiological studies. However, there is no known mechanism for biological effects of weak environmental MFs. Previous studies indicate MF effects on DNA integrity and reactive oxygen species, but such evidence is limited to MFs higher (greater than or equal to 100 µT) than those generally found in the environment. Effects of 10 and 30 µT fields were studied in SH-SY5Y and C6 cells exposed to 50-Hz MFs for 24 h. Based on earlier findings, menadione (MQ) was used as a cofactor. Responses to MF were observed in both cell lines, but the effects differed between the cell lines. Micronuclei were significantly increased in SH-SY5Y cells at 30 µT. This effect was largest at the highest MQ dose used. Increased cytosolic and mitochondrial superoxide levels were observed in C6 cells. The effects on superoxide levels were independent of MQ, enabling further mechanistic studies without co-exposure to MQ. The micronucleus and mitochondrial superoxide data were consistent with a conventional rising exposure–response relationship. For cytosolic superoxide, the effect size was unexpectedly large at 10 µT. The results indicate that the threshold for biological effects of ELF MFs is 10 µT or less.

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