Biological effects of 6 mT static magnetic fields: A comparative study in different cell types

2006 ◽  
Vol 27 (7) ◽  
pp. 560-577 ◽  
Author(s):  
Bernadette Tenuzzo ◽  
Alfonsina Chionna ◽  
Elisa Panzarini ◽  
Remigio Lanubile ◽  
Patrizia Tarantino ◽  
...  
Keyword(s):  
Magnetic Fields ◽  
Comparative Study ◽  
Biological Effects ◽  
Cell Types ◽  
Static Magnetic Fields ◽  
Different Cell Types

scholarly journals Comparative Analysis of Biological Effects Induced on Different Cell Types by Magnetic Fields with Magnetic Flux Densities in the Range of 1–60 mT and Frequencies up to 50 Hz

2018 ◽  
Vol 10 (8) ◽  
pp. 2776 ◽  
Author(s):  
Cristian Vergallo ◽  
Luciana Dini
Keyword(s):  
Magnetic Fields ◽  
Magnetic Flux ◽  
Transmission Lines ◽  
Bystander Effect ◽  
Human Peripheral Blood ◽  
Biological Effects ◽  
Low Frequency ◽  
Cell Types ◽  
Peripheral Blood Lymphocytes ◽  
Different Cell Types

Moderate static magnetic fields (SMFs) are generated from sources such as new-generation electric trams and trains, electric arc welding, and magnetic resonance imaging (MRI) devices, as well as during the industrial production of aluminium, while extremely low frequency pulsed magnetic fields (ELF-PMFs) are produced by house power installations, household appliances, and high voltages transmission lines. Moderate SMFs and ELF-PMFs with magnetic flux densities (B) in the range of 1–60 mT and frequencies (f) up to 50 Hz are common MF exposure sources for the population. Even though humans are continually exposed to these MFs, to date no definitive endpoint has been drawn about their safety. In this review, the state of knowledge about the biological effects induced by these MFs on different cell types will be addressed. In our own observation, the putative modulation of Ca2+/H+ and Na+/H+ plasma membrane antiporters of human peripheral blood lymphocytes (PBLs) was found to occur after a 24 h exposure to a 6 mT SMF, and the bystander effect observed on U937 cells cultivated for up to 6 h in the conditioned medium harvested from human PBLs previously exposed for 24 h to the same MF (secondary necrosis induction) will be also herein discussed.

scholarly journals Cell shape and plasma membrane alterations after static magnetic fields exposure

10.4081/840 ◽  
2009 ◽  
Vol 47 (4) ◽  
pp. 299 ◽  
Author(s):  
A Chionna ◽  
M Dwikat ◽  
E Panzarini ◽  
B Tenuzzo ◽  
EC Carlà ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Magnetic Fields ◽  
Cell Surface ◽  
Cell Shape ◽  
Biological Effects ◽  
Human Lymphocytes ◽  
Cell Types ◽  
Continuous Exposure ◽  
U937 Cells ◽  
Static Magnetic Fields

The biological effects of static magnetic fields (MFs) with intensity of 6 mT were investigated in lymphocytes and U937 cells in the presence or absence of apoptosis-inducing drugs by transmission (TEM) and scanning (SEM) electron microscopy. Lectin cytochemistry of ConA-FITC conjugates was used to analyze plasma membrane structural modifications. Static MFs modified cell shape, plasma membrane and increased the level of intracellular [Ca++] which plays an antiapoptotic role in both cell types. Modifications induced by the exposure to static MFs were irrespective of the presence or absence of apoptotic drugs or the cell type. Abundant lamellar-shaped microvilli were observed upon 24 hrs of continuous exposure to static MFs in contrast to the normally rough surface of U937 cells having numerous short microvilli. Conversely, lymphocytes lost their round shape and became irregularly elongated; lamellar shaped microvilli were found when cells were simultaneosly exposed to static MFs and apoptosis-inducing drugs. In our experiments, static MFs reduced the smoothness of the cell surface and partially impeded changes in distribution of cell surface glycans, both features being typical of apoptotic cells. Cell shape and plasma membrane structure modifications upon static MFs exposure were time-dependent. Lamellar microvilli were clearly observed before the distortion of cell shape, which was found at long times of exposure. MFs exposure promoted the rearrangement of F-actin filaments which, in turn, could be responsible for the cell surface modifications. Here we report data that support biological effects of static MFs on U937 cells and human lymphocytes. However, the involvement of these modifications in the onset of diseases needs to be further elucidated.

Transcriptional activation by the human Hsp70-associating protein Hap50

2001 ◽  
Vol 114 (10) ◽  
pp. 1839-1845
Author(s):  
Y. Niyaz ◽  
M. Zeiner ◽  
U. Gehring
Keyword(s):  
Transcriptional Activation ◽  
Fluorescent Protein ◽  
Biological Effects ◽  
Cell Types ◽  
Mrna Stabilization ◽  
Activation Of Transcription ◽  
Enhanced Expression ◽  
Endogenous Genes ◽  
Green Fluorescent ◽  
Different Cell Types

We investigated human Hap50, the large isoform of the previously characterized Hsp70/Hsc70-associating protein Hap46, also called BAG-1, for effects on transcriptional activities. Overproduction by transient transfection led to enhanced expression of reporter gene constructs in various cell types using different promoters, suggesting independence of promoter type. Similarly, overexpression of Hap50 resulted in increased levels of poly(A)(+)mRNAs in HeLa, COS-7, 3T3 and HTC cells. Concomitantly, the expression of some selected endogenous genes, such as those coding for c-Jun and the glucocorticoid receptor, was enhanced significantly relative to actin. Nuclear runoff transcription assays using HeLa cells showed that the effect is caused by increased transcription rates rather than mRNA stabilization. Activation of transcription by Hap50 occurred at 37 degrees C and did not require prior thermal stress, as is the case for Hap46. In accordance with these biological effects, Hap50 is localized exclusively in the nuclear compartment of different cell types, whereas Hap46 is mostly cytoplasmic in unstressed cells, as revealed by use of fusion constructs with green fluorescent protein. High cellular levels of Hap50 were found to make cells less susceptible to adverse environmental effects such as heat stress. Our data suggest that Hap50 is a nuclear protein that acts in cells to increase the transcription of various genes.

Biological effects of static magnetic fields and zinc isotopes onE. colibacteria

2018 ◽  
Vol 40 (1) ◽  
pp. 62-73 ◽  
Author(s):  
Ulyana G. Letuta ◽  
Vitaly L. Berdinskiy
Keyword(s):  
Magnetic Fields ◽  
Biological Effects ◽  
Static Magnetic Fields ◽  
Zinc Isotopes

Model study of biological effects of weak static magnetic fields at the organismic and subcellular levels

2015 ◽  
Vol 461 (1) ◽  
pp. 116-119 ◽  
Author(s):  
V. E. Stefanov ◽  
B. F. Shchegolev ◽  
O. V. Kriyachko ◽  
N. V. Kuzmenko ◽  
S. V. Surma ◽  
...  
Keyword(s):  
Magnetic Fields ◽  
Biological Effects ◽  
Static Magnetic Fields ◽  
Model Study
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