scholarly journals Interactions of Arbuscular Mycorrhizal Fungi with Hyphosphere Microbial Communities in a Saline Soil: Impacts on Phosphorus Availability and Alkaline Phosphatase Gene Abundance

Soil Systems ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 63
Author(s):  
Abdurrahman Masrahi ◽  
Anil Somenahally ◽  
Terry Gentry
Keyword(s):  
Alkaline Phosphatase ◽  
Microbial Community ◽  
Root Colonization ◽  
Saline Soil ◽  
Saline Soils ◽  
P Availability ◽  
Community Interactions ◽  
Gene Abundance ◽  
Alp Activity ◽  
Plant P Uptake

The limited availability of soil phosphorus to plants under salinity stress is a major constraint for crop production in saline soils, which could be alleviated by improving mycorrhizal and soil microbial interactions. This study investigated the effects of Funneliformis mosseae (Fm) inoculation on phosphorus (P) availability to Sorghum bicolor, and alkaline phosphatase (ALP) activity and gene abundance (phoD) in a P-deficient naturally saline soil. A greenhouse study was conducted in order to compare the experimental treatments of Fm inoculated vs. control plants grown in saline soil with and without (sterilized soil) native microbial community. A separate hyphosphere (root-free) compartment was constructed within the mycorrhizosphere and amended with phosphate. After four weeks of transplanting, shoot, roots, mycorrhizosphere, and hyphosphere samples were collected and analyzed for soil and plant P concentrations, root colonization, and abundance of ALP and phoD. The results showed significantly higher colonization in Fm-inoculated treatments compared to uninoculated. Plant available P concentrations, phoD gene abundance and ALP activity were significantly reduced (p < 0.05) in sterilized-hyphosphere as compared to unsterilized in both Fm-inoculated and uninoculated treatments. Inoculation with Fm significantly increased the plant P uptake (p < 0.05) when compared to uninoculated treatments, but only in the plants gown in unsterile mycorrhizosphere. It can be concluded that inoculation of Fm increased root colonization and the uptake of P by sorghum plant in saline soil and native microbial community interactions were critical for increasing bioavailable P concentrations. These beneficial interactions between plants, mycorrhizae, and native microbes should be considered for soil fertility management in saline soils.

scholarly journals Bioprocess Optimization for the Production of Arthrospira (Spirulina) platensis Biomass Enriched in the Enzyme Alkaline Phosphatase

2021 ◽  
Vol 8 (10) ◽  
pp. 142
Author(s):  
Giorgos Markou
Keyword(s):  
Alkaline Phosphatase ◽  
Single Cell ◽  
Protein Content ◽  
Fold Increase ◽  
Arthrospira Platensis ◽  
P Availability ◽  
Alp Activity ◽  
P Limitation ◽  
Protein Rich ◽  
Enzyme Alkaline Phosphatase

The enzyme alkaline phosphatase (ALP) is gaining interest because it exerts bioactive properties and may be a potentially important therapeutic agent for many disorders and diseases. Microalgae are considered an important novel source for the production of diverse bio-compounds and are gaining momentum as functional foods/feeds supplements. So far, studies for the production of ALP are limited to mammalian and partly to some heterotrophic microbial sources after its extraction and/or purification. Methods: Arthrospira was cultivated under P-limitation bioprocess and the effect of the P-limitation degree on the ALP enrichment was studied. The aim of this work was to optimize the cultivation of the edible and generally-recognized-as-safe (GRAS) cyanobacterium Arthrospira platensis for the production of single-cell (SC) biomass enriched in ALP as a potential novel functional diet supplement. Results: The results revealed that the relationship between intracellular-P and single-cell alkaline phosphatase (SC-ALP) activity was inverse; SC-ALP activity was the highest (around 50 U g−1) when intracellular-P was the lowest possible (around 1.7 mg-P g−1) and decreased gradually as P availability increased reaching around 0.5 U g−1 in the control cultures. Under the strongest P-limited conditions, a more than 100-fold increase in SC-ALP activity was obtained; however, protein content of A. platensis decreased significantly (around 22–23% from 58%). Under a moderate P-limitation degree (at intracellular-P of 3.6 mg-P g−1), there was a relatively high SC-ALP activity (>28 U g−1) while simultaneously, a relative high protein content (46%) was attained, which reflects the possibility to produce A. platensis enriched in ALP retaining though its nutritional value as a protein rich biomass source. The paper presents also results on how several parameters of the ALP activity assay, such as pH, temperature etc., and post-harvest treatment (hydrothermal treatment and biomass drying), influence the SC-ALP activity.

Evaluation of Spectrophotometric and Fluorometric Methods for Alkaline Phosphatase Activity Determination in Ewe's Milk

2000 ◽  
Vol 63 (9) ◽  
pp. 1258-1261 ◽  
Author(s):  
M. F. SCINTU ◽  
E. DAGA ◽  
A. LEDDA
Keyword(s):  
Alkaline Phosphatase ◽  
Standard Deviation ◽  
Raw Milk ◽  
Cow’S Milk ◽  
Official Method ◽  
Cow's Milk ◽  
Alp Activity ◽  
Relative Standard ◽  
Ewe's Milk ◽  
European Method

The alkaline phosphatase (ALP) activity test has been used since 1935 to assess the effectiveness of pasteurization. Different analytical methods exist for detecting ALP in milk. Unfortunately, there is little information about ALP activity in ewe's milk. The aim of this study was to assess and compare the official European method (spectrophotometric method) and the Fluorophos method (fluorometric method) regarding their use in ewe's milk. Bulk ewe's milk samples were taken from a flock and from three different dairies. A portion of the original sample was pasteurized at 63°C for 30 min in a circulating bath; another portion was heated to and kept at 95°C for about 2 min, and 0.1% (vol/vol) of raw milk was added. The samples obtained were analyzed in duplicate using the spectrophotometric and fluorometric methods. The relation between ALP activity determined by the two methods was characterized by the following equation: Y = 1.34 + 0.0039X (where Y = ALP in μg of phenol per ml of milk and X = ALP in mU/liter; R2 = 91.5%). Precision parameters (repeatability [r], standard deviation of repeatability [sr], and relative standard deviation of repeatability [RSDr]) for both methods were calculated. The values of RSDr for the Fluorophos method were 4.30 for pasteurized milk and 2.96 for 0.1% raw milk, close to the value indicated by Rocco in whole cow's milk (RSDr = 4.4). The repeatability for the official method (r = 2.16) was close to that indicated for whole cow's milk (r = 2).

Residual Alkaline Phosphatase Activity in Milks Subjected to Various Time-Temperature Treatments

1997 ◽  
Vol 60 (5) ◽  
pp. 525-530 ◽  
Author(s):  
C. J. PAINTER ◽  
R. L. BRADLEY
Keyword(s):  
Alkaline Phosphatase ◽  
Activity Levels ◽  
Milk Product ◽  
Milk Products ◽  
Alp Activity ◽  
High Temperature Short Time ◽  
Thermally Processed ◽  
Long Time ◽  
The Mean ◽  
Fluid Milk

Milk is routinely tested for proper pasteurization. The Scharer and Fluorophos methods, among others, test for residual alkaline phosphatase (ALP) activity to assure proper pasteurization. Until recently there were no tests available to accurately detect residual ALP activity levels below the U.S. legal limit of 1 μg of phenol or 350 mU of ALP per liter of milk. The new Fluorophos method can detect accurately residual ALP activity levels as low as 10 mU/liter. The Fluorophos method was used to investigate residual ALP activity levels in several fluid milk products. The milk products were thermally processed under various time and temperature protocols below, at, and above current U.S. Food and Drug Administration-mandated heat treatments for fluid milk and milk products. The data established values for residual ALP activity in milks pasteurized under high-temperature short-time (HTST) and low-temperature long-time (LTLT) treatments. The mean ALP activities for whole, 2% lowfat, 1% lowfat, skim, half and half, and chocolate-flavored milks thermally processed at the legal minimum HTST pasteurization treatment are 169.7 ± 12.3, 145.2 ± 9.3, 98.6 ± 8.9, 72.5 ± 4.2, 38.4 ± 4.6 and 157.3 ± 6.5 mU/liter, respectively. The mean ALP activities generated at the legal minimum LTLT pasteurization treatment are 81.8 ± 4.8, 66.4 ± 5.9, 56.4 ± 2.1, 39.1 ± 3.9, 35.0 ± 1.2 and 91.3 ± 7.7 mU/liter, respectively. The values for all milks pasteurized at the legal minimum heat treatment were significantly below the current legal cutoff for residual ALP activity of 350 mU/liter of milk or milk product.

Alkaline Phosphatase Isoenzyme Patterns in Malignant Disease

1992 ◽  
Vol 38 (12) ◽  
pp. 2546-2551 ◽  
Author(s):  
V O Van Hoof ◽  
A T Van Oosterom ◽  
L G Lepoutre ◽  
M E De Broe
Keyword(s):  
Alkaline Phosphatase ◽  
Liver Disease ◽  
Liver Metastasis ◽  
Bone Metastasis ◽  
Positive Predictive Value ◽  
Bone Disease ◽  
Predictive Value ◽  
Malignant Disease ◽  
Alp Activity ◽  
Isoenzyme Patterns

Abstract Early treatment of patients with malignant disease and liver or bone metastasis may increase their survival time. We have used the activity patterns of liver and bone isoenzymes of alkaline phosphatase (ALP), separated by agarose gel electrophoresis, to detect early metastasis. We studied ALP isoenzyme patterns in a background population of 101 patients with no evidence of any disease that might influence this pattern; a healthy reference population (n = 330); and the following three groups of patients: 143 with malignant disease, 47 with nonmalignant liver disease, and 22 with nonmalignant bone disease. Cutoff and predictive values of liver ALP, high-molecular-mass (high-M(r)) ALP, and bone ALP were established for detecting liver and bone metastasis. The positive predictive value of liver and high-M(r) ALP was higher than that of total ALP in detecting liver metastasis, but liver and high-M(r) ALP did not enable us to differentiate between malignant and nonmalignant liver disease. Total ALP activity was of slightly more value than liver and high-M(r) ALP in enabling us to rule out liver metastasis. From bone ALP activity we could not distinguish between nonmalignant bone disease and bone metastasis. The negative predictive value of bone ALP in the diagnosis of bone metastasis was low, but its positive predictive value was high and superior to that of total ALP.

Development of Ca2+ homeostasis in epithelial cells from embryonic and neonatal intestine

1992 ◽  
Vol 263 (3) ◽  
pp. G371-G379
Author(s):  
B. L. Black ◽  
J. O. Rogers
Keyword(s):  
Alkaline Phosphatase ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Fluorescent Probe ◽  
Cell Suspensions ◽  
Fura 2 ◽  
Alp Activity ◽  
High Viability ◽  
Cytoplasmic Compartment ◽  
Highly Correlated

The fluorescent probe fura-2 was used to assay Ca2+ levels in epithelial cell suspensions from embryonic and neonatal chick duodenum. Cell preparations maintained high viability, completely hydrolyzed fura-2/AM to fura-2, retained 92% of cellular fura-2 within the cytoplasmic compartment, and gave low autofluorescence values during assay. Fura-2 leakage from loaded cells occurred at all ages, but could be corrected for in subsequent calculations of cellular Ca2+. Cytoplasmic Ca2+ concentration was 76-80 nM in cells from 14- to 16-day embryonic intestine, rose significantly to 92-98 nM at 17-20 days, and reached 209 nM at 1-day post-hatch when assayed in buffers containing 1.3 mM Ca2+. The developmental rise in cytoplasmic Ca2+ was accompanied by an enhanced ability of cells to maintain a constant Ca2+ concentration at increased levels of extracellular Ca2+ and by a highly correlated rise in alkaline phosphatase (ALP) activity. Epithelial Ca2+ subsequently decreased to the "adult" value of 133-142 nM and was constant along the crypt-villus axis of neonatal intestine. These results verify that fura-2 can be used to compare baseline cytoplasmic Ca2+ values of epithelial cells from developing intestine, reveal that significant changes in Ca2+ homeostasis occur during ontogeny, and suggest that epithelial Ca2+ may modulate ALP activity during the differentiation of embryonic enterocytes.

Interleukin-1β enhances retinoic acid-mediated expression of bone-type alkaline phosphatase in rat IEC-6 cells

2001 ◽  
Vol 280 (3) ◽  
pp. G510-G517 ◽  
Author(s):  
Takeshi Nikawa ◽  
Madoka Ikemoto ◽  
Kaori Tokuoka ◽  
Shigetada Teshima ◽  
David H. Alpers ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Retinoic Acid ◽  
Retinoic Acid Receptor ◽  
Transcript Level ◽  
Intestinal Epithelial ◽  
Alp Activity ◽  
Kinase C ◽  
Fetal Rat ◽  
Epidermal Growth

We previously showed that vitamin A upregulated the expression of bone-type alkaline phosphatase (ALP) in fetal rat small intestine and rat intestinal IEC-6 cells. In this study, we examined interactions between retinoic acid (RA) and several growth factors/cytokines on the isozyme expression in IEC-6 cells. Epidermal growth factor and interleukins (ILs)-2, -4, -5, and -6 completely blocked the RA-mediated increase in ALP activity. In contrast, IL-1β markedly increased the activity, protein, and mRNA of the bone-type ALP only when RA was present. IL-1β and/or RA did not change the type 1 IL-1 receptor transcript level, whereas IL-1β enhanced the RA-induced expressions of retinoic acid receptor-β (RAR-β) and retinoid X receptor-β (RXR-β) mRNAs and RA-mediated RXR response element binding. The synergism of IL-1β and RA on ALP activity was completely blocked by protein kinase C (PKC) inhibitors. Our results suggest that IL-1β may modify the ALP isozyme expression in small intestinal epithelial cells by stimulating PKC-dependent, RAR-β- and/or RXR-β-mediated signaling pathways.

Sign in / Sign up

Export Citation Format

Share Document