Identification of ginsenoside markers from dry purified extract of Panax ginseng by a dereplication approach and UPLC–QTOF/MS analysis

2015 ◽  
Vol 109 ◽  
pp. 91-104 ◽  
Author(s):  
Heejung Yang ◽  
Dong Young Lee ◽  
Kyo Bin Kang ◽  
Jeom Yong Kim ◽  
Sun Ok Kim ◽  
...  
Keyword(s):  
Separations ◽  
2021 ◽  
Vol 8 (4) ◽  
pp. 53
Author(s):  
Clarissa Braccia ◽  
Bhakti Prinsi ◽  
Mara Colzani ◽  
Alessandra A. Altomare ◽  
Luca Espen ◽  
...  

The benefits of ginseng have been mainly attributed to its triterpenoids, called ginsenosides. Recent genome sequencing of the Panax ginseng has paved the way for in-depth proteomic studies of this medicinal plant. The current study was conducted to deepen the proteomic information on the root proteome of Korean ginseng. Proteomic workflow was optimized by testing two different strategies, characterized by the phenol extraction procedure, the presence or the absence of SDS-PAGE fractionation step, and nano-scale liquid chromatographic tandem mass spectrometry (nLC-MS/MS) analysis. The results highlighted an evident improvement of proteome extraction by the combination of phenol extraction with SDS-PAGE before the nLC-MS/MS analysis. In addition, a dramatic impact of the steaming process (the treatment to produce red ginseng from ginseng) on protein properties was observed. Overall, the analyses of Korean ginseng permitted the characterization of a total of 2412 proteins. A large number of identified proteins belonged to the functional categories of protein and carbon/energy metabolism (22.4% and 14.6%, respectively). The primary and secondary metabolisms are major metabolic pathways, which emerged from the proteomic analysis. In addition, a large number of proteins known to play an important role in response to (a)biotic stresses were also identified. The current proteomic study not only confirmed the previous transcriptomic and proteomic reports but also extended proteomic information, including the main metabolic pathways involved in Korean ginseng.


2020 ◽  
Vol 150 ◽  
pp. 695-704
Author(s):  
Xiaoxue Fang ◽  
Difu Zhu ◽  
Yinghong Chen ◽  
Lianlian Song ◽  
Ruizhi Jiang ◽  
...  

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
JH Lee ◽  
JH Lee ◽  
YM Lee ◽  
PN Kim ◽  
CS Jeong

2011 ◽  
Vol 49 (05) ◽  
Author(s):  
S Szabó ◽  
L Márk ◽  
S Kiss ◽  
É Polyák ◽  
A Kisbenedek ◽  
...  
Keyword(s):  

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
N Fabre ◽  
E Deharo ◽  
HL Le ◽  
C Girardi ◽  
A Valentin ◽  
...  

Planta Medica ◽  
2013 ◽  
Vol 79 (10) ◽  
Author(s):  
S Dobreniecki ◽  
JR Porter
Keyword(s):  

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
HN Jeong ◽  
KJ Lee ◽  
JS Jeong ◽  
YM Mo ◽  
SJ Lee ◽  
...  
Keyword(s):  

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
HH Kim ◽  
BJ Seong ◽  
KS Lee ◽  
SI Kim ◽  
JM Geun ◽  
...  

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